Rapid change in climate and land use are having substantial impacts on lakes around the world. Here, we synthesized time-series data for dissolved oxygen, temperature, chlorophyll-a, total phosphorus, total nitrogen, and dissolved organic carbon at multiple depths in 827 lakes to facilitate analyses of these interconnected changes. The dataset extends from 1938–2022, with a median data duration of 29 years (range 2–80) and a median of XXX profiles per year at each lake. Lakes in the dataset have a median depth of 14.0 m (range 6.4–370 m), median surface area of 95.1 ha (range: 1.1–126909 ha) and median elevation of 242 m (range: -215–2804). The lakes are located in 20 countries across 5 continents, with latitudes ranging from -42.6 to 68.3. To facilitate interoperability with other large-scale datasets, each lake is linked to a unique hydroLAKES lake ID when possible.