Rapid changes in climate and land use are having substantial impacts on lakes around the world. Here, we synthesized time-series data for dissolved oxygen, temperature, chlorophyll-a, total phosphorus, total nitrogen, and dissolved organic carbon at multiple depths in 827 lakes to facilitate analyses of these interconnected changes. The dataset extends from 1921–2022, with a median data duration of 29 years (range 5-102) and a median of 5 unique sampling dates per year at each lake. Lakes in the dataset have a median depth of 12.5 m (range 1.5–480 m), median surface area of 85.4 ha (range: 0.5–237000 ha) and median elevation of 264 m (range: -215–2804). The lakes are located in 18 countries across 5 continents, with latitudes ranging from -42.6 to 68.3. To facilitate interoperability with other large-scale datasets, each lake is linked to a unique hydroLAKES lake ID when possible (n = 683).