Laboratory experiments were conducted to investigate impacts of pH, alkalinity, and presence of particles on Mn removal in freshwater. The dataset includes monitoring data from: 1) a 14-day experiment in Mn(II) solutions in nanopure water, 2) a 24-hour experiment in Mn(II) solutions in nanopure water, and 3) a 10-day experiment in water from two drinking water reservoirs. The 14-day pH and alkalinity laboratory experiment was conducted starting October 30, 2022 and included sample collection and pH monitoring on day 0, 1, 4, 7, 10, and 14. The 24-hour pH and alkalinity laboratory experiment was conducted starting February 20, 2023 and included sample collection and pH monitoring at 0, 1, 2, 6, 12, and 24 hours. The reservoir water laboratory experiment was conducted starting March 22, 2023 and included sample collection and pH monitoring on day 0, 1, 4, 7, and 10. This experiment tested Mn removal in water collected from the lower water column of Falling Creek Reservoir (FCR) and Carvins Cove Reservoir (CCR), located in Vinton, Virginia, USA and Roanoke, Virginia, USA respectively. Both reservoirs are owned and operated by the Western Virginia Water Authority and are managed as drinking-water sources for the city of Roanoke, VA, USA.