We identified variables controlling stream nitrogen concentrations and fluxes, and how they have changed over time , by synthesizing 20 time series ranging from 5 to 51 years of data collected from forest and grassland dominated watersheds across Europe, North America, and East Asia and across four climate types (tropical, temperate, Mediterranean, and boreal) using the International Long-Term Ecological Research Network. We found declining trends in bulk ammonium and nitrate deposition, with ammonium contributing significantly more to atmospheric nitrogen deposition over time. Among sites, there were significant positive relationships between (1) precipitation and stream ammonium and nitrate fluxes and (2) atmospheric nitrogen inputs and stream nitrogen concentrations and fluxes. There were no significant relationships between air temperature and stream nitrogen export. Our long-term data shows that although nitrogen deposition is declining over time, atmospheric nitrogen inputs and precipitation remain the main predictors for nitrogen exported from forested and grassland watersheds. Overall, we also demonstrate that long-term monitoring provides understanding of ecosystems and biogeochemical cycling that would not be possible with short-term studies alone.