The Interagency Ecological Program’s (IEP) Summer Townet Survey (STN) and Fall Midwater Trawl (FMWT) are two long-term monitoring projects conducted by the California Department of Fish and Wildlife (CDFW) to monitor fish abundance and distribution trends in the San Francisco Estuary (SFE) since 1959 and 1967, respectively. Starting in 2005, zooplankton monitoring was added and paired with fish tows to investigate food availability for young fishes. Food limitation has been a long-term issue and a focus of the Pelagic Organism Decline (POD) studies that began in 2005. By 2011, STN routinely conducted zooplankton monitoring at 40 stations, and FMWT at 32 stations in the upper SFE from Carquinez Strait to the Sacramento Deep Water Ship Channel and into the South Delta. STN samples every other week from June to August and FMWT samples once monthly from September to December. Both projects collect mesozooplankton samples using a modified Clarke-Bumpus (CB) net to target copepods and cladocerans, and FMWT also samples macrozooplankton (i.e. mysids and amphipods) using a mysid net. Flowmeters are used to measure the volume sampled to determine zooplankton catch per unit effort. Environmental variables such as water temperature, turbidity, secchi, and electrical conductivity are collected with each zooplankton sample. Concurrent fish and zooplankton tows conducted by STN and FMWT have allowed for comparisons of fish diet to the available zooplankton prey at the time of collection.