These are amplicon sequencing data collected during Plumes and Blooms (PnB) cruises conducted from March, 2011, through September, 2014. The V9 hypervariable region of the 18S rRNA gene derived from microbial eukaryotic communities was amplified and sequenced from 345 discrete seawater samples. Sample collection and laboratory methods are described in Catlett et al. 2020 and Catlett et al. in review. Bioinformatic and data manipulation methods follow those employed in Catlett et al. in review. The data are provided in two tables: one includes amplicon sequence variant (ASV) sequences and relative sequence abundances for each sampling event, and the other includes ASV taxonomy predictions for each ASV sequence.
References:
Catlett, D., P. G. Matson, C. A. Carlson, E. G. Wilbanks, D. A. Siegel, and M. D. Iglesias‐Rodriguez. 2020. Evaluation of accuracy and precision in an amplicon sequencing workflow for marine protist communities. Limnol. Oceanogr.: Methods. 18(1): 20-40. https://doi.org/10.1002/lom3.10343.
Catlett, D., D. A. Siegel, P. G. Matson, E. K. Wear, C. A. Carlson, T. S. Lankiewicz, and M. D. Iglesias‐Rodriguez. In review. Integrating phytoplankton pigment and DNA meta-barcoding observations to determine phytoplankton community composition in the coastal ocean. Limnol. Oceanogr.