A pressure transducer (CS450, Campbell Scientific Inc., Logan, UT, USA) and a multi-parameter water quality sonde (YSI Series 6:6920 v2, YSI Inc., Yellow Springs, OH, USA) were deployed by the Virginia Tech Biological Systems Engineering (BSE) Stream Research, Education, and Management (StREAM) Lab (vtstreamlab.weebly.com/) from December 2012 to December 2018 to assess stage and water quality, and calculate discharge in Stroubles Creek, Blacksburg, VA (4118326.215 N, 549236.02 W). Stroubles Creek is a 19 km tributary of the New River in Blacksburg, Virginia, USA. Stroubles Creek begins immediately below the Virginia Tech campus and flows north into the Kanawha River in the Valley and Ridge physiographic province. Stroubles Creek was a historic water supply for the town of Blacksburg and remains a highly visible waterway in the town, as it traverses both the downtown area and much of the Virginia Tech campus. In 2002, Stroubles Creek was placed on Virginia’s 303(d) list of impaired water bodies with a benthic impairment by the Virginia Department of Environmental Quality (VADEQ). A total maximum daily load (TMDL) plan was subsequently developed in 2003 for the stream in collaboration with Virginia Tech’s BSE department, the Virginia Department of Environmental Quality, and the Virginia Department of Conservation and Recreation. During 2008-2010, a 2.1 km reach of Stroubles Creek, mostly upstream of the StREAM Lab, was restored by means of cattle exclusion, natural revegetation, bank reshaping, and creating an inset floodplain. The water quality dataset provided here is associated with the upper Stroubles Creek watershed which drains approximately 14.5 km2. Currently, data from the StREAM Lab are used by 8-10 faculty and their graduate students in 7 departments across 5 colleges at Virginia Tech for research and at least 16 classes for field labs and/or assignments.