This data package, LAGOS-US LANDSAT, is one of the extension data modules of the LAGOS-US platform that provides six water quality estimates (chlorophyll, Secchi depth, dissolved organic carbon, total suspended solids, turbidity, and true water color) from remote sensing for lakes ≥ 4 ha in the conterminous U.S. (48 states plus the District of Columbia) for the years 1984-2020. These estimates are generated through machine learning models on in-lake water quality matchups from LAGOS-US LIMNO with Landsat 5, 7, and 8 whole lake median reflectance values and pixel-wise band ratios that are subsequently used to make predictions across the U.S. The LANDSAT module contains remotely sensed reflectance values for 136,977 of the 137,465 lakes ≥ 4 ha from the LAGOS-US research platform. Within the module are a total of 45,867,023 sets of reflectance values, a matchup dataset with a window of up to 7 calendar days with in situ data, and associated water quality parameter predictions for each reflectance set. Additional quality control flags are provided for predictions indicating whether reflectance extractions included negative values, the percent of the maximum pixels ever retrieved for that lake that the predictions are based on, and whether there are shared calendar day predictions due to scene overlap.