This data set includes spatially explicit mark-recapture data of the Northern Spring Salamander (Gyrinophilus porphyriticus) collected during the summer months (June – August) from downstream and upstream reaches in multiple streams in the Hubbard Brook Experimental Forest. Downstream reaches begin at the confluence with the Main Hubbard and extend upstream 500 meters and upstream reaches begin at the weir and extend downstream 500 meters. Downstream reaches contain brook trout and upstream reaches do not. We used a robust design framework with approximately 9 surveys per reach each summer (3 primary occasions with 3 secondary occasions each). Salamanders were captured by hand and marked with either Visual Implant Elastomer and/or a PIT tag.