A full understanding of the contemporary status of lake dissolved oxygen and thermal habitat in the face of global change is enhanced by high-frequency monitoring that goes beyond periodic water column profiles of temperature and dissolved oxygen. The data contained herein were used to examine the relationship between both concurrent warming and increasing dissolved organic carbon (DOC) concentrations, and oxythermal habitat status for cold-water species. To obtain high resolution oxythermal habitat characteristics, fifteen lakes in the Adirondack Park of northern New York state were monitored from mid-June to mid-October of 2021. Lakes were outfitted with high-frequency temperature and dissolved oxygen logger chains located in most cases at the deepest point of each lake. Temperature loggers were placed throughout the water column while dissolved oxygen loggers were positioned to best capture low-oxygen portions of the water column based on data from previous years. To enhance vertical resolution, high-frequency data were supplemented with periodic water column profiles. In addition, to provide a comprehensive regional perspective, we include historical DOC and depth data from more than 1,400 Adirondack lakes. Finally, to enhance understanding of fish response to temperature, we include body temperature data from tagged brook trout for two of our intensively monitored lakes during part of the 2023 stratified period.