Identifying the scaling rules describing ecological patterns across time and space is a central challenge in ecology. Taylor’s Law of fluctuation scaling, which states that the variance of a population is proportional to a positive power of the mean, has been widely observed in population dynamics and characterizes variability in multiple scientific domains. However, it is unclear if this phenomenon accurately describes ecological patterns across many orders of magnitude in time, and therefore links otherwise disparate observations. This dataset uses light attenuation observations from 10,531 days of high-frequency measurements in 35 globally distributed lakes to test this unknown. We focus on water clarity as an integrative ecological characteristic that responds to both biotic and abiotic drivers. We provide documentation that variations in ecological measurements across diverse sites and temporal scales exhibit variance patterns consistent with Taylor’s Law, and that model coefficients increase in a predictable yet non-linear manner with decreasing observation frequency.