The information includes biotic and ecological index data of benthic macroinvertebrates collected in the Ecuadorian Amazon Region. The biotic indexes are taxonomic abundance, richness, evenness, diversity, and dominance. The ecological indexes refer to the ecological water quality as determined by pollution-tolerant macroinvertebrates. The datasets also include physicochemical parameters for water quality determination. The dataset has been completed,; however, it may be updated if new information is generated.