University of the Ozarks stream ecology students collected biotic (macroinvertebrate and fish community) and abiotic (temperature, pH, conductivity, dissolved oxygen, discharge) data in the spring 2024 semester. Macroinvertebrate data was collected using kick nets, D-nets, and visual inspection, and fish were collected using seine nets. Temperature is being logged in perpetuity with a HOBO, MX2201 data logger, and pH, conductivity, and dissolved oxygen were recorded using a YSI Quatro handheld multiparameter probe. River discharge was calculated manually using the surface float method, and a qualitative habitat assessment was conducted. This data package is a long-term, temporal, student-led data generation effort designed to be shared with the freshwater community.