Water-soluble organic matter (WSOM) represents organic matter that has the potential to be readily released from soils. WSOM has been understudied in urban, engineered soils relative to natural soils. To understand the potential for organic matter and nutrient release, we extracted WSOM from the soils of stormwater control measures (SCM) and urban wetlands. In February 2022, we sampled soils from 20 SCMs and natural wetlands in the Rappahannock River watershed of the mid-Atlantic United States. The SCMs reflected a variety of design configurations including bioretention, rain gardens, wet ponds, and swales. We also sampled naturally occurring floodplain wetlands that are located in this urban watershed. Soils were sampled to a depth of approximately 40 cm. If there was standing water present in the SCMs and wetlands at the time of sampling, we also collected surface water samples. If present, grab samples of leaf litter or biomass were collected. Soil characteristics, such as pH, bulk density, soil moisture, soil organic matter, and cation exchange capacity were also determined for each site. WSOM was extracted from soils and biomass in the laboratory and analyzed for organic matter concentration (dissolved organic carbon) and composition (absorbance and fluorescence metrics), along with dissolved nutrient concentrations (total dissolved nitrogen, total dissolved phosphorus, nitrate, ammonium, and orthophosphate). In addition to the 20 sites in the Rappahannock watershed, soils from 2 additional bioretention SCMs on the Virginia Tech campus were sampled on a monthly basis from February 2022 to February 2023 to explore temporal variability in WSOM. To characterize changes in soil hydrologic conditions during monthly SCM sampling, we applied a Thornthwaite-type monthly water balance model. Finally, we performed a simple scaling exercise to WSOM results based on SCM area, sample depth, and soil bulk density to estimate potential SCM contributions of organic matter.