This dataset contains information on soil physical and chemical characteristics across twenty urban reforestation sites planted as part of the Reforest the Bluegrass program in Lexington, Kentucky, USA. At least three plots (and up to nine plots) were established in each site, with additional plots added if forested patches were sufficiently large. Soil samples were collected from each plot in summer 2020. Samples were composited from five subsamples collected from plot center and approximately 1 m from plot center in the four cardinal directions. Subsamples were collected to a depth of approximately 10 cm using a sampling spade. Composite samples were air-dried and passed through a 2cm sieve, and then sent to the University of Kentucky Regulatory Services soils lab for analysis for pH, P, K, Ca, Mg, Zn, particle size distribution, total C, and total N.