This data package contains biomass data for annual or perennial grasses and forbs harvested from quadrats along the Jornada Basin LTER (LTER-I) transects in southern New Mexico, USA. These transects are located in a livestock exclosure established in 1982 in the Chihuahuan Desert Rangeland Research Center (CDRRC) and run from the middle of the College Playa up to the foot of Mt. Summerford (2.7 km in length). Prior to the exclosure, the study site was moderately to heavily grazed for the past 100 years. The Treatment transect was treated annually with ammonium nitrate fertilizer (NH4NO3 at 10g N/m2/yr) until 1987. Along each transect, 91 stations, each with a plant intercept line, are spaced at 30 meter intervals. Each plant intercept line is perpendicular to the transect and is 30 meters in length broken into 5-meter segments. The included dataset includes biomass data for grasses and forbs clipped from 1 square meter quadrats directly downslope from some, but not all, of these segments. The harvest measurements occurred in September 1989. Data consists of the date, transect, station number, quad, JRN and USDA Plants species codes, species binomial, habit, form, and biomass. This study is complete.