This data package contains nitrogen mineralization data from soils collected along the Jornada Basin LTER (LTER-I) transects in southern New Mexico, USA. These transects are located in a livestock exclosure established in 1982 in the Chihuahuan Desert Rangeland Research Center (CDRRC) and run from the middle of the College Playa up to the foot of Mt. Summerford (2.7 km in length). Prior to the exclosure, the study site was moderately to heavily grazed for the past 100 years. The Treatment transect was treated annually with ammonium nitrate fertilizer (NH4NO3 at 10g N/m2/yr) until 1987. Along each transect, 91 stations, each with a plant intercept line, are spaced at 30 meter intervals. For this dataset, 60 soil samples (total) were collected along the control and fertilized treatment transects and mixed with potassium chloride solution (KCl) on Nov 27, 1989, then filter extracted the following day. The dataset contains a soil moisture correction factor, sample weights, total inorganic nitrogen (NO3+NO2-N), and nitrogen in ammonium (NH4-N) for Week F (field) of nitrogen mineralization potentials. The soil mineralization data complements the biomass harvest measurements that occurred in September 1989 (dataset knb-lter-jrn.210015001). This study is complete.