This dataset contains plant and litter cover estimates derived from overhead photos collected from microplots in a long-term experiment (2013-present) of cross-scale interactions (CSIs) at the Jornada Basin LTER site in southern New Mexico, U.S.A. Experimental treatments were initiated in 2013 at 15 experimental blocks, each with 4 treatment plots: plant-scale herbicide of mesquite shrubs, patch-scale connectivity modifiers (ConMods), herbicide + ConMods, control without manipulations. Repeat, overhead (downward-looking) photographs of ten "microplots" in each plot were taken for estimation of litter, soil, and vegetation cover in the experimental treatment and control plots over time. Photographs were rotated and then cropped to provide standardized areas, and then were analyzed with USDA SamplePoint software to determine coverage by ~27 plant, litter or other cover classes on a 100 point grid. Raw and corrected cover estimates are provided. This study is ongoing and new data will be added annually. Cover estimates are derived from photos in EDI dataset knb-lter-jrn.210413004.