To examine BSC (biological soil crust) biotic and abiotic compositions, three BSC types (cyanolichen, dark algal, light algal) were collected during the summer 2017, fall 2017, and spring 2018 seasons to discern differences between two vegetation states (grass and tarbush). A total of 51 biocrusts were collected from 3 tarbush sites and 3 grass sites. Phospholipid fatty acid analysis was used to characterize the microfloral community, while a modified extraction method was employed to observe direct counts of microfauna (multicellular microbes and protists). Soil chemical analysis was also used to obtain nutrient concentrations of each biocrust.