Chlorophyll is measured at our permanent sampling station in the deepest part of the lake. Chlorophyll samples are collected from the four primary study lakes in the Madison area (Lakes Mendota, Monona, and Wingra and Fish Lake) at integrated depths and discrete depths for spectrophotometric analysis and fluorometric analysis. Due to a change in instruments starting in 2002 and lasting through 2007, chlorophyll analyses for the southern lakes had an uncorrectable bias, and are not included in this dataset. Analyses since then (2008 onward) have been determined to not have this bias. Sampling Frequency: bi-weekly during ice-free season from late March or early April through early September, then every 4 weeks through late November; sampling is conducted usually once during the winter (depending on ice conditions). Number of sites: 4