Dissolved organic matter (DOM) is a complex mixture of organic compounds found in all natural waters. Its composition affects its reactivity towards numerous processes. Its composition is a function of both its source (e.g., allochthonous or autochthonous) as well as the extent of environmental processing it has undergone (e.g., chemical or biological degradation). Ultraviolet-visible (UV-vis) spectroscopy is an analytical technique commonly used to assess the composition of dissolved organic matter in water samples. Here, we present spectra from Lake Mendota samples collected from June - November in 2017 at the surface of Lake Mendota as well as at specific depths within the water column. All samples were collected near the NTL-LTER research buoy. Absorbance values are listed for wavelengths 200 - 800 nm for each sample.