Data package associated with Handler et al. (2024) "Nitrate loads from land to stream are balanced by in-stream nitrate uptake across season in a dryland stream". The study describes the nitrate dynamics in Oak Creek watershed. Data include measurements from four seasonal synoptic sampling campaigns, nine seasonal stream nitrate uptake experiments on the main stem and tributaries, and the results of a network model that estimates the lateral load of nitrate from surrounding landscape to the stream network as well as network-level stream nitrate uptake and retention.