Fish communities in desert streams are shaped by both the long-term selective pressures of streamflow regimes and the short-term effects of floods and droughts. Between spring 2016 and spring 2019 we conducted seasonal sampling of nine streams across the state of Arizona, USA on a gradient of flow regime variability. We calculated populations size for each species at all surveys to analyze seasonal and long-term variations in fish community composition. We additionally used the data to calculate annual rates of fish biomass production. This data set includes the raw catch data from three pass depletion electrofishing, population size estimates at each season, sampling locations, and the USGS stream gauges we used to obtain streamflow data.