This data set is a record since 1986 of chemistry for surface water draining the S2 catchment at the Marcell Experimental Forest (MEF) in Itasca County, Minnesota. Unfiltered water is usually collected every one or two weeks as part of the long-term monitoring program of the S2 catchment. Some samples were collected more often for various other studies and are included in this data set. Samples are routinely measured for pH, specific conductivity, anions (chloride, sulfate), cations (calcium, magnesium, potassium, sodium, aluminum, iron, manganese, strontium), silicon, nutrients (ammonium, nitrate+nitrite, soluble reactive phosphorus, total nitrogen, total phosphorus), and total organic carbon. Occasionally, stable water and mercury isotopes as well as concentrations of dissolved organic carbon (DOC), bacterial respiration of dissolved organic matter, biodegradable DOC (BDOC), ferrous and ferric iron, total mercury (filtered or unfiltered), methylmercury (filtered or unfiltered), and lead were measured. Ultraviolet (UV) absorbance, a measure of water color or dissolved organic matter optical properties, was also measured for some samples. More solutes and values will be added as additional metadata are documented (pre-1986 to 1992), water samples are collected and analyzed (concentrations and isotopes), or archived water samples are analyzed for stable water isotopes. The MEF is operated and maintained by the USDA Forest Service, Northern Research Station.