While there is strong evidence of diversity effects on temporal variability of productivity, whether this mechanism extends to variability across space remains elusive. Here, we present data from Nutrien Network (www.nutnet.org) that were used to determine the relationship between diferent scales of plant diversity and spatial variability of productivity in 83 grasslands worldwide, and to quantify the effect of experimentally increased spatial heterogeneity in environmental conditions on this relationship. There are two data sets, one for the pre-treatment (observational_data.csv) data, and other for the experimentally increased heterogeneity (increased heterogeneity.csv). In these data sets, study sites contained at least three replicates that originated from blocks each composed of ten 5 m × 5 m plots. In addition, pre-treatment data has a subset of sites in where soil conditions where measured (observational_data_soil.csv) and a version in which data for each site are sumarized and site-level climatic variables obtained from WorldClim (www.worldclim.org) are added. (observational_data_site_climate.csv). If you need any clarification or further information, please contact us.