These files contain microhabitat, soil, vegetation structure, and woody plant species data used in the paper "Grassland woody plant management rapidly changes woody vegetation persistence and abiotic habitat conditions but not herbaceous community composition". The project was conducted at seven publicly accessible remnant (i.e., unplowed or old-growth) tallgrass prairie within 100 miles of Madison, Wisconsin, United States starting in the 2020 growing season and commencing following the 2022 growing season. The goal was to assess the initial effects of different management interventions on woody vegetation persistence, abiotic habitat conditions, and herbaceous community composition, including physical and chemical management interventions and their combination.