Monitoring of egg thiamine levels was initiated in California hatcheries in 2020 after thiamine deficiency complex (an insufficiency of vitamin B1) was first documented in this system resulting in widespread mortality of juveniles. Unfertilized eggs (~10g) from mature adult females were collected from populations of Chinook salmon (Oncorhynchus tshawytscha), Steelhead trout (Oncorhynchus mykiss), and coho salmon (Oncorhynchus kisutch) across California to assess thiamine concentrations. Concurrent fork length, weight, as well as coded wire tag recovery information was recorded alongside egg thiamine concentration levels. Additionally, laboratory investigations have been undertaken to uncover the relationship between egg thiamine concentration and the survival of offspring. Utilizing these findings, we have understood the potential population-level impacts of thiamine-dependent mortalities based on egg surveillance data. The annual monitoring of eggs from salmonid species in both Central Valley and coastal populations serves as a critical component in comprehending the prevalence, magnitude, and tends of this deficiency among California salmonids. This dataset is focused on supporting research and providing in season data to inform the public and research managers responding to this emerging threat. Given the value of this information to managing salmon resources and treatments, we expect this monitoring to continue into the future if funding allows.