Most research on stream metabolism uses a single reach to characterize a stream. We aimed to characterize variation in stream metabolism rates and the controls on metabolism along a 75-km agricultural stream. We continuously measured dissolved oxygen, discharge, conductivity, temperature, light, and turbidity using a variety of sensors deployed at 6 locations along Marsh Creek, a stream in southeastern Idaho. Monthly grab samples were collected for water chemistry throughout the study period, and aquatic vegetation was surveyed from May through September. Data were collected from October 2016 through October 2017.