Fatty Acid profiles were obtained for various periphyton sources (food for consumers) accross the freshwater Everglades in June-July 2016, the peak of the wet season, and in January 2017, the peak of the dry season. These data include specific fatty acid markers for each periphyton type (floating mat, benthic mat, epiphyton, and/or filamentous green) collected. Nutritional measurements for various periphyton sources (food for consumers) were also obtained during the same intervals. These measurements include periphyton type (floating mat, benthic mat, epiphyton, and/or filamentous green), periphyton availability (volume, organic content and mineral content), stoichiometric data (TP, C:N, C:P, and N:P), and macronutrient composition (protein, carbohydrate, lipid). These measurements form the basis for the data discussed in the dissertation chapter, "Freshwater herbivores dominate over omnivores in suboptimal habitats because of their ability to exploit low quality resources" by Jessica L Sanchez and Joel C Trexler.