Annual (water year basis) geometric mean concentrations of total phosphorus (TP), soluble-reactive phosphorus (SRP), total nitrogen (TN), dissolved inorganic nitrogen (DIN; calculated as nitrate + nitrite + ammonia), chlorophyll-a (Chl-a), and total organic carbon (TOC) concentrations across Everglades National Park (ENP), Florida Keys National Marine Sanctuary (FKNMS), West Florida Shelf and Florida Bay. This dataset is composed of data from multiple sources including Florida Coastal Everglades, Florida International University Southeast Research Center (FIU SERC), South Florida Water Management District (SFWMD), and National Oceanic and Atmospheric Administration Atlantic Oceanographic and Meteorological Laboratory (NOAA AOML). All values reported less than the laboratory minimum detection limit (MDL) were set to one-half the MDL. Annual geometric mean concentrations were computed for monitoring locations with greater than five years of data and four samples per year with a minimum of one sample in the wet and dry seasons. This dataset was created to evaluate long-term spatial and temporal trends in nutrients, chlorophyll-a, and total organic carbon at the landscape scale relative to freshwater and marine ecosystems.