Data Package Metadata   View Summary

Interlaboratory testing of CuSO4 toxicity in the “Standardized Aquatic Microcosm” protocol consisting of multiple phytoplankton and animals in a chemically defined medium.

General Information
Data Package:
Local Identifier:edi.1083.1
Title:Interlaboratory testing of CuSO4 toxicity in the “Standardized Aquatic Microcosm” protocol consisting of multiple phytoplankton and animals in a chemically defined medium.
Alternate Identifier:DOI PLACE HOLDER
Abstract:

Four different laboratories conducted a total of ten experiments of the “Standardized Aquatic Microcosm” to test the reproducibility of results to control, low, medium, and high concentrations of CuSO4. In nine experiments, treatments consisted of six replicates of 0, 500, 1000, and 2000 ppb Cu++. One experiment, ME74, used 0, 127, 255, 509 ppb. The purpose was to test a chemically defined medium (thus negating differences due to local water supplies) and the same 10 species of phytoplankton and 5 animals, including Daphnia. Microbes were undefined. The protocol included the weekly re-introduction of small numbers of each species to allow potential recovery from toxicity. Control microcosms had a “spring algal bloom” terminated by zooplankton grazing and multiple competitive interactions. The copper inhibited some phytoplankton more than others and killed many grazers, especially Daphnia.

The data set presented several interesting statistical properties that would yield new insights.

  • (a) The results were very similar, but the timing varied— the higher the concentration of copper, the longer the inhibition and mortality of organisms, so those at 500 ppb recovered earlier, the 1000 ppb recovered later, and at 2000 ppb most never recovered. But if compared on each sampling day, e.g., 10, 14, … to 64, results appear highly variable.
  • (b) In at least one experiment, the toxicity of copper was challenging to demonstrate statistically because high variability in the timing of recovery of the intermediate concentration increased pooled variances.
  • (c) The elimination of highly-sensitive dominant organisms allowed less-sensitive organisms to increase in abundance. Within natural environments, the observation that some species increase in the presence of toxic substances has been used to discredit toxicity testing without considering the relative sensitivities of competing or predatory species.
  • (d) The competitive interactions among organisms, e.g., cyanobacteria and green algae, may provide new insights.

More data are available than fit the format, and those interested should contact taub@uw.edu.

Related papers

Conquest, L. L., and F. B. Taub. 1989. Repeatability and reproducibility of the standardized aquatic microcosm: Statistical properties. Pages 159-177 in A. S. 1027, editor. Aquatic Toxicology and Hazard Assessment: 12th Volume. American Society for Testing and Materials, Philadelphia.

Harrass, M. C., and F. B. Taub. 1985. Comparison of laboratory microcosms and field responses to copper. Pages 57-74 in T. P. Boyle, editor. Validation and Predictability of Laboratory Methods for Assessing the Fate and Effects of Contaminants in Aquatic Ecosystems, ASTM STP 865. American Society for Testing and Materials, Philadelphia.

Kindig, A. C., F. B. Taub, and L. L. Conquest. 1984. Interlaboratory repeatability of experimental effects in laboratory microcosms.in 8th ASTM Symposium on Aquatic Toxicology, Fort Mitchell, Kentucky.

Meador, J. P. 1988. The Chemical and Biological Factors Associated with Ecosystem Recovery from Copper Stress as Exemplified by Microcosms. University of Washington https://www.proquest.com/dissertations-theses/chemical-biological-factors-associated-with/docview/303592933/se-2?accountid=14784

Meador, J. P., T. H. Sibley, G. L. Swartzman, and F. B. Taub. 1998. Resistance to copper toxicity by the freshwater algal species Oocystis pusilla and its ability to alter free ion copper. Aquatic Toxicology 44:69-82. https://doi.org/10.1016/S0166-445X(98)00067-8

Meador, J. P., F. B. Taub, and T. H. Sibley. 1993. Copper dynamics and the mechanism of ecosystem level recovery in a standardized aquatic microcosm. Ecological Applications 3:139-155. https://doi.org/1941797

Rose, K. A., G. L. Swartzman, A. C. Kindig, and F. B. Taub. 1988. Stepwise iterative calibration of a multispecies phytoplankton-zooplankton simulation model using laboratory data. Ecological Modelling 42:1-32. https://doi.org/10.1016/0304-3800(88)90089-0

Swartzman, G. L., F. B. Taub, J. Meador, C. Huang, and A. C. Kindig. 1990. Modeling the effect of algal biomass on multispecies aquatic microcosms response to copper toxicity. Aquatic Toxicology 17:93-118. https://doi.org/10.1016/0166-445X(90)90025-K

Taub, F. 1989. Standardized aquatic microcosm development and testing. Pages 47-94 in A. Boudou and F. Ribeyre, editors. Aquatic Ecotoxicology. CRC Press, Inc. https://www.taylorfrancis.com/chapters/edit/10.1201/9781351069854-3/standardized-aquatic-microcosm-development-testing-taub

Taub, F. 1983. Direct and indirect effects of test chemicals displayed by synthetic, aquatic microcosms. Presented at the Society of Enivronmental Toxicology and Chemistry (SETAC), 2nd Annual Meeting, Arlington, Virginia.

Taub, F. B. 1985. Toward interlaboratory (round-robin) testing of a standardized aquatic microcosm. Pages 165-186 in J. Cairns Jr, editor. Multispecies Toxicity Testing. Pergamon Press, U.K.

Taub, F. B. 1993. Standardizing an Aquatic Microcosm Test. Pages 159-188 in A. Soares and P. Calow, editors. Progress in Standardization of Aquatic Toxicity Tests. Pergamon Press.

Taub, F. B. 1997a. Are ecological studies relevant to pesticide registration decisions? Ecological Applications 7:1083-1085. https://www.jstor.org/stable/2641194

Taub, F. B. 1997b. Unique information contributed by multispecies systems: Examples from the standardized aquatic microcosm. Ecological Applications 7:1103-1110. https://doi.org/2641198

Taub, F. B., A. C. Kindig, and L. L. Conquest. 1986. Preliminary results of interlaboratory testing of a standardized aquatic microcosm. Pages 93-120 in J. Cairns Jr, editor. Community Toxicity Testing. American Society for Testing Materials, Philadelphia.

Taub, F. B., A. C. Kindig, and L. L. Conquest. 1988a. Interlaboratory testing of a standardized aquatic microcosm. Pages 384-405 in W. J. Adams, G. A. Chapman, and W. G. Landis, editors. Aquatic Toxicology and Hazard Assessment. American Society for Testing Materials, Philadelphia.

Taub, F. B., A. C. Kindig, L. L. Conquest, and J. P. Meador. 1988b. STANDARDIZED AQUATIC MICROCOSMS - ECOLOGICAL RESPONSES TO TOXICANTS. Aquatic Toxicology 11:422-423. https://doi.org/10.1016/0166-445X(88)90124-5

Taub, F. B., A. C. Kindig, L. L. Conquest, and J. P. Meador. 1989. Results of interlaboratory testing of the standardized aquatic microcosm protocol. Pages 368-394 in G. W. Suter II and M. A. Lewis, editors. Aquatic Toxicology and Hazard Assessment, ASTM STP 1007. American Society for Testing Materials, Philadelphia.

Taub, F. B., A. C. Kindig, J. P. Meador, and G. L. Swartzman. 1991. Effects of â seasonal successionâ nand grazing on copper toxicity in aquatic microcosms. SIL Proceedings, 1922-2010 24:2205-2214. https://doi.org/10.1080/03680770.1989.11899926

Taub, F. B., P. L. Read, A. C. Kindig, M. C. Harrass, H. J. Hartmann, L. L. Conquest, F. J. Hardy, and P. T. Munro. 1983. Demonstration of the ecological effects of streptomycin and malathion on synthetic aquatic microcosms. Pages 5-25 in W. E. Bishop, R. D. Cardwell, and B. B. Heidolph, editors. Aquatic Toxicology and Hazard Assessment: Sixth Symposium. American Society for Testing and Materials, Philadelphia, PA.

Publication Date:2022-02-17
For more information:
Visit: DOI PLACE HOLDER

Time Period
Begin:
1983-12-02
End:
1986-07-18

People and Organizations
Contact:Taub, Frieda B (School of Aquatic and Fishery Sciences - University of Washington, Professor Emeritus) [  email ]
Contact:Matheson, John (US FDA retired) [  email ]
Contact:Meador, James (NOAA Fisheries, Aquatic Toxicologist) [  email ]
Creator:Taub, Frieda B (University of Washington, Professor Emeritus)

Data Entities
Data Table Name:
Experiment Specific Metadata
Description:
Experiment specific data such as location, personal, start and end data, and lab notes.
Data Table Name:
ME64
Description:
All data for experiment 64 from the University of Washington in Seattle with six replicates of 0, 500, 1000, and 2000 ppb Cu++.
Data Table Name:
ME65
Description:
All data for experiment 65 from EPA Duluth Laboratory in Minnesota with six replicates of 0, 500, 1000, and 2000 ppb Cu++.
Data Table Name:
ME67
Description:
All data for experiment 67 from EPA Duluth Laboratory in Minnesota with six replicates of 0, 500, 1000, and 2000 ppb Cu++.
Data Table Name:
ME69
Description:
All data for experiment 69 from the University of Washington in Seattle with six replicates of 0, 500, 1000, and 2000 ppb Cu++.
Data Table Name:
ME72
Description:
All data for experiment 72 from the University of Washington in Seattle with six replicates of 0, 500, 1000, and 2000 ppb Cu++.
Data Table Name:
ME74
Description:
All data for experiment 74 from the Aberdeen-Army lab in Maryland with six replicates of 0, 127, 255, and 509 ppb Cu++.
Data Table Name:
ME79
Description:
All data for experiment 79 from the Marine Bioassay Laboratories, Inc. in California with six replicates of 0, 500, 1000, and 2000 ppb Cu++.
Data Table Name:
ME82
Description:
All data for experiment 82 from the University of Washington in Seattle with six replicates of 0, 500, 1000, and 2000 ppb Cu++.
Data Table Name:
ME87
Description:
All data for experiment 87 from the Army Aberdeen lab in Maryland with six replicates of 0, 500, 1000, and 2000 ppb Cu++. This experiment does not contain nutrient data.
Data Table Name:
ME89
Description:
All data for experiment 89 from the University of Washington in Seattle with six replicates of 0, 500, 1000, and 2000 ppb Cu++.
Detailed Metadata

Data Entities


Data Table

Data:https://pasta-s.lternet.edu/package/data/eml/edi/1083/1/a035923ae7674314e001853634ae30a3
Name:Experiment Specific Metadata
Description:Experiment specific data such as location, personal, start and end data, and lab notes.
Number of Records:10
Number of Columns:7

Table Structure
Object Name:Metadata by Experiment.csv
Size:12702 byte
Authentication:3a5b25f65a75fbf959fbaf5b1ee52afa Calculated By MD5
Text Format:
Number of Header Lines:1
Record Delimiter:\r\n
Orientation:column
Simple Delimited:
Field Delimiter:,
Quote Character:"

Table Column Descriptions
 Experiment NumberTreatmentStart DateEnd DatePersonnelLocationLab Notes
Column Name:Experiment Number  
Treatment  
Start Date  
End Date  
Personnel  
Location  
Lab Notes  
Definition:Assigned experiment number as labeled.Description of treatments used on each experiment and if the experiment was replaced with another.Day zero of the experiment.Day 63 of the experiment.A list of personnel who worked on a given experiment.Location of the lab were the experiment was done.Notes taken during the experiment.
Storage Type:float  
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dateTime  
dateTime  
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Measurement Type:nominalnominaldateTimedateTimenominalnominalnominal
Measurement Values Domain:
DefinitionAssigned experiment number as labeled.
DefinitionDescription of treatments used on each experiment and if the experiment was replaced with another.
FormatYYYY-MM-DD
Precision
FormatYYYY-MM-DD
Precision
DefinitionA list of personnel who worked on a given experiment.
DefinitionLocation of the lab were the experiment was done.
DefinitionNotes taken during the experiment.
Missing Value Code:              
Accuracy Report:              
Accuracy Assessment:              
Coverage:              
Methods:              

Data Table

Data:https://pasta-s.lternet.edu/package/data/eml/edi/1083/1/deaea71fee9891a3dace23ca37013d90
Name:ME64
Description:All data for experiment 64 from the University of Washington in Seattle with six replicates of 0, 500, 1000, and 2000 ppb Cu++.
Number of Records:456
Number of Columns:56

Table Structure
Object Name:ME64.csv
Size:188794 byte
Authentication:8b442f4e5b52f9235eb4622383b9a2f5 Calculated By MD5
Text Format:
Number of Header Lines:1
Record Delimiter:\r\n
Orientation:column
Simple Delimited:
Field Delimiter:,
Quote Character:"

Table Column Descriptions
 DayTreatmentMicrocosm NumberSelenastrum DensityChlamydomonas DensityScenedesmus DensityAnkistrodesmus DensityChlorella DensityNitzschia Kutzigiana DensityAnabaena Cell DensityLyngbya Cell DesntiyStigeoclonium DensityUlothrix DensityUnidentified Green Alga DensityAvailable AlgaeAlgal BiovolumeDaphnia Fecundity RatioShannon-Weaver Weighted Algal DiversityShannon-Weaver Algal Species DiversitySmall Daphnia ConcentrationMedium Daphnia ConcentrationLarge Daphnia ConcentrationDaphnia ConcentrationSmall to Total Daphnia Concentration RatioDaphnia BiomassSmall Amphipods ConcentrationLarge Amphipods ConcentrationAmphipods ConcentrationOstracods ConcentrationEphippia PresenceRotifer ConcentrationSmall Rotifer ConcentrationHypotrichs ConcentrationMiscellaneous Protozoan ConcentrationVorticella CocentrationNemertines ConcentrationFirst Dissolved Oxygen MeasurementSecond Dissolved Oxygen MeasurementThird Dissolved Oxygen MeasurementDissolved Oxygen GainDissolved Oxygen LossDissolved Oxygen Change Over 24 HoursNet Photosynthesis-Respiration RatioIn vivo FluorescencepH Immediately After Lights OnpH at 1130-1200 HoursPhosphate ConcentrationSilicate ConcentrationNitrate ConcentrationNitrite ConcentrationAmmonia ConcentrationNitrate to Phosphate ratio(Nitrate + Nitrite) to Phosphate RatioTotal Nitrogen to Phosphate Ratio
Column Name:DAY  
TRT  
MIC  
SELAN  
CHLAMY  
SCENED  
ANKIS  
CHLOR  
D216  
ANABENA  
LYNGBYA  
STIG  
ULOTH  
UNKALG1  
AVALGAE  
ABIOVOL  
DFECUND  
DVRSFD  
DVRSALG  
SDAPHNIA  
MDAPHNIA  
LDAPHNIA  
DAPHNIA  
PSDAPHN  
MDSIZE  
DBIOMAS  
SAMPH  
LAMPH  
AMPH  
OSTRACOD  
EPHIPPIA  
PHILODIN  
ROTIFER  
HYPO  
MISCEL  
VORT  
NEMER  
TURBAB  
DO1  
DO2  
DO3  
DDOAM  
DDOPM  
DELDO  
RATIOPR  
FLUOR  
PH1  
PH2  
PO4  
SIO4  
NO3  
NO2  
NH3  
RATIO1  
RATIO2  
RATIO3  
Definition:Day since experiment started the sample was takenThe treatment number assigned to microcosm the data was collected fromMicrocosm number (identifier)Selenastrum abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Chlamydomonas abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Scenedesmus abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Ankistrodesmus abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Chlorella abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Nitzschia Kutzigiana abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Anabaena abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Lyngbya abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Stigeoclonium abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Ulothrix abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Unidentified green alga abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.A measurement of the algae available for Daphnia to feed on based on the biomass of algae and their feeding availability.The measurement of the total algal biovolume of the algae in each microcosm determined by algae count and cell volume.Daphnia Fecundity Ratio. Calculated by SDAPHNIA/LDAPHNIA.An estimate of the diversity of algal cells that are available to the Daphnia for feedingDiversity measurements of all algal species from the Shannon-Weaver equation.Small Daphnia(~0.7mm) count per 100mLMedium Daphnia(0.7mm-1.8mm) count per 100mLLarge Daphnia(>1.8mm) count per 100mLDaphnia count per 100mLThe ratio of small to total daphniaMean Daphnia LengthDaphnia Biomass per 100MLSmall Amphipods(<4mm) per 100 mLLarge Amphipods(>4mm) per 100 mLAmphipods per 100 mLOstracods per 100mLEphippia presence in DaphniaPhilodina acuticornis (rotifer) per mLSmall Rotifers per mLHypotrichs (protozoa) counts per mLMiscellaneous Protozoan per mLVorticella(Protozoa) counts per mLNemertines per mLAbsorbance at 440nM in Spectrophotometer Model 20, which provides a measure of turbidity.First measurement of dissolved oxygen taken in the a.m. before the lights are turned on.Second measurement of dissolved oxygen taken in the p.m. before the lights are turned off.Third measurement of dissolved oxygen taken in the a.m. before the lights are turned on.The amount of gained dissolved oxygen during the light period. This is calculated by subtracting DO1 from DO2.The amount of lost dissolved oxygen during the dark period. This is calculated by subtracting DO3 from DO2.The change in dissolved oxygen over 24 hours. This is calculated by subtracting DO1 from DO3.The ratio of photosynthesis(gain of oxygen) and respiration(loss of oxygen). The equation is as follows: (DO2-DO1)/(DO2-DO3) = (DDOAM)/(DDOPM)In vivo Fluorescence measured in 1-cm diameter tube. Measured using Turner Model #111 fluorometer.pH taken immediately after lights are turned on at 930 hours.pH taken between 1130-1200 hours.Phosphate(PO4) concentrationSilicate(SiO4) concentrationNitrate NO3 ConcentrationNitrite(NO2) ConcentrationAmmonia(NH3) ConcentrationRatio of Nitrate to Phosphate. Calculated by NO3/PO4.Ratio of Nitrate and Nitrite to Phosphate. Calculated by (NO3 + NO2)/PO4.Total Nitrogen to Phosphate Ratio. Calculated by (NO3+NO2+NH3)/PO4.
Storage Type:float  
string  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
string  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
string  
float  
string  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
Measurement Type:rationominalnominalratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratiorationominalratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratio
Measurement Values Domain:
UnitnominalDay
Typereal
Allowed Values and Definitions
Enumerated Domain 
Code Definition
Code1
DefinitionControl
Source
Code Definition
Code2
Definition500 ppb Cu^++
Source
Code Definition
Code3
Definition1000 ppb Cu^++
Source
Code Definition
Code4
Definition2000 ppb Cu^++
Source
DefinitionMicrocosm number (identifier)
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberMicrometerCubedPer0.1Mililiter
Typereal
UnitnumberMicrometerCubedPer0.1MilimeterCubed
Typereal
Unitdimensionless
Typereal
Unitdimensionless
Typereal
Unitdimensionless
Typereal
UnitnumberPer100Milliliters
Typereal
UnitnumberPer100Milliliters
Typereal
UnitnumberPer100Milliliters
Typereal
UnitnumberPer100Milliliters
Typereal
Unitdimensionless
Typereal
Unitmillimeter
Typereal
UnitnumberPer100Milliliters
Typereal
UnitnumberPer100Milliliters
Typereal
UnitnumberPer100Milliliters
Typereal
UnitnumberPer100Milliliters
Typereal
UnitnumberPer100Milliliters
Typereal
Allowed Values and Definitions
Enumerated Domain 
Code Definition
Code-99
DefinitionData is not available
Source
Code Definition
Code0
DefinitionEphippia is not present
Source
Code Definition
Code1
DefinitionEphippia is present
Source
UnitnumberPerMilliliter
Typereal
UnitnumberPerMilliliter
Typereal
UnitnumberPerMilliliter
Typereal
UnitnumberPerMilliliter
Typereal
UnitnumberPerMilliliter
Typereal
UnitnumberPerMilliliter
Typereal
UnitopticalDensity
Typereal
UnitpartsPerMillion
Typereal
UnitpartsPerMillion
Typereal
UnitpartsPerMillion
Typereal
UnitpartsPerMillion
Typereal
UnitpartsPerMillion
Typereal
UnitpartsPerMillion
Typereal
Unitdimensionless
Typereal
Unitdimensionless
Typereal
Unitdimensionless
Typereal
Unitdimensionless
Typereal
UnitmicrogramPerLiter
Typereal
UnitmicrogramPerLiter
Typereal
UnitmicrogramPerLiter
Typereal
UnitmicrogramPerLiter
Typereal
UnitmicrogramPerLiter
Typereal
Unitdimensionless
Typereal
Unitdimensionless
Typereal
Unitdimensionless
Typereal
Missing Value Code:      
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
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Code-99.0000
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Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Accuracy Report:                                                                                                                
Accuracy Assessment:                                                                                                                
Coverage:                                                                                                                
Methods:                                                                                                                

Data Table

Data:https://pasta-s.lternet.edu/package/data/eml/edi/1083/1/3bfbd30603c63c2c9ed4ed9f6c1ef12f
Name:ME65
Description:All data for experiment 65 from EPA Duluth Laboratory in Minnesota with six replicates of 0, 500, 1000, and 2000 ppb Cu++.
Number of Records:456
Number of Columns:56

Table Structure
Object Name:ME65.csv
Size:189055 byte
Authentication:f44e06b4835bfca680e3a9d64a452bd3 Calculated By MD5
Text Format:
Number of Header Lines:1
Record Delimiter:\r\n
Orientation:column
Simple Delimited:
Field Delimiter:,
Quote Character:"

Table Column Descriptions
 DayTreatmentMicrocosm NumberSelenastrum DensityChlamydomonas DensityScenedesmus DensityAnkistrodesmus DensityChlorella DensityNitzschia Kutzigiana DensityAnabaena Cell DensityLyngbya Cell DesntiyStigeoclonium DensityUlothrix DensityUnidentified Green Alga DensityAvailable AlgaeAlgal BiovolumeDaphnia Fecundity RatioShannon-Weaver Weighted Algal DiversityShannon-Weaver Algal Species DiversitySmall Daphnia ConcentrationMedium Daphnia ConcentrationLarge Daphnia ConcentrationDaphnia ConcentrationSmall to Total Daphnia Concentration RatioDaphnia BiomassSmall Amphipods ConcentrationLarge Amphipods ConcentrationAmphipods ConcentrationOstracods ConcentrationEphippia PresenceRotifer ConcentrationSmall Rotifer ConcentrationHypotrichs ConcentrationMiscellaneous Protozoan ConcentrationVorticella CocentrationNemertines ConcentrationFirst Dissolved Oxygen MeasurementSecond Dissolved Oxygen MeasurementThird Dissolved Oxygen MeasurementDissolved Oxygen GainDissolved Oxygen LossDissolved Oxygen Change Over 24 HoursNet Photosynthesis-Respiration RatioIn vivo FluorescencepH Immediately After Lights OnpH at 1130-1200 HoursPhosphate ConcentrationSilicate ConcentrationNitrate ConcentrationNitrite ConcentrationAmmonia ConcentrationNitrate to Phosphate ratio(Nitrate + Nitrite) to Phosphate RatioTotal Nitrogen to Phosphate Ratio
Column Name:DAY  
TRT  
MIC  
SELAN  
CHLAMY  
SCENED  
ANKIS  
CHLOR  
D216  
ANABENA  
LYNGBYA  
STIG  
ULOTH  
UNKALG1  
AVALGAE  
ABIOVOL  
DFECUND  
DVRSFD  
DVRSALG  
SDAPHNIA  
MDAPHNIA  
LDAPHNIA  
DAPHNIA  
PSDAPHN  
MDSIZE  
DBIOMAS  
SAMPH  
LAMPH  
AMPH  
OSTRACOD  
EPHIPPIA  
PHILODIN  
ROTIFER  
HYPO  
MISCEL  
VORT  
NEMER  
TURBAB  
DO1  
DO2  
DO3  
DDOAM  
DDOPM  
DELDO  
RATIOPR  
FLUOR  
PH1  
PH2  
PO4  
SIO4  
NO3  
NO2  
NH3  
RATIO1  
RATIO2  
RATIO3  
Definition:Day since experiment started the sample was takenThe treatment number assigned to microcosm the data was collected fromMicrocosm number (identifier)Selenastrum abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Chlamydomonas abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Scenedesmus abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Ankistrodesmus abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Chlorella abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Nitzschia Kutzigiana abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Anabaena abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Lyngbya abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Stigeoclonium abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Ulothrix abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Unidentified green alga abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.A measurement of the algae available for Daphnia to feed on based on the biomass of algae and their feeding availability.The measurement of the total algal biovolume of the algae in each microcosm determined by algae count and cell volume.Daphnia Fecundity Ratio. Calculated by SDAPHNIA/LDAPHNIA.An estimate of the diversity of algal cells that are available to the Daphnia for feedingDiversity measurements of all algal species from the Shannon-Weaver equation.Small Daphnia(~0.7mm) count per 100mLMedium Daphnia(0.7mm-1.8mm) count per 100mLLarge Daphnia(>1.8mm) count per 100mLDaphnia count per 100mLThe ratio of small to total daphniaMean Daphnia LengthDaphnia Biomass per 100MLSmall Amphipods(<4mm) per 100 mLLarge Amphipods(>4mm) per 100 mLAmphipods per 100 mLOstracods per 100mLEphippia presence in DaphniaPhilodina acuticornis (rotifer) per mLSmall Rotifers per mLHypotrichs (protozoa) counts per mLMiscellaneous Protozoan per mLVorticella(Protozoa) counts per mLNemertines per mLAbsorbance at 440nM in Spectrophotometer Model 20, which provides a measure of turbidity.First measurement of dissolved oxygen taken in the a.m. before the lights are turned on.Second measurement of dissolved oxygen taken in the p.m. before the lights are turned off.Third measurement of dissolved oxygen taken in the a.m. before the lights are turned on.The amount of gained dissolved oxygen during the light period. This is calculated by subtracting DO1 from DO2.The amount of lost dissolved oxygen during the dark period. This is calculated by subtracting DO3 from DO2.The change in dissolved oxygen over 24 hours. This is calculated by subtracting DO1 from DO3.The ratio of photosynthesis(gain of oxygen) and respiration(loss of oxygen). The equation is as follows: (DO2-DO1)/(DO2-DO3) = (DDOAM)/(DDOPM)In vivo Fluorescence measured in 1-cm diameter tube. Measured using Turner Model #111 fluorometer.pH taken immediately after lights are turned on at 930 hours.pH taken between 1130-1200 hours.Phosphate(PO4) concentrationSilicate(SiO4) concentrationNitrate NO3 ConcentrationNitrite(NO2) ConcentrationAmmonia(NH3) ConcentrationRatio of Nitrate to Phosphate. Calculated by NO3/PO4.Ratio of Nitrate and Nitrite to Phosphate. Calculated by (NO3 + NO2)/PO4.Total Nitrogen to Phosphate Ratio. Calculated by (NO3+NO2+NH3)/PO4.
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float  
Measurement Type:rationominalnominalratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratiorationominalratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratio
Measurement Values Domain:
UnitnominalDay
Typereal
Allowed Values and Definitions
Enumerated Domain 
Code Definition
Code1
DefinitionControl
Source
Code Definition
Code2
Definition500 ppb Cu^++
Source
Code Definition
Code3
Definition1000 ppb Cu^++
Source
Code Definition
Code4
Definition2000 ppb Cu^++
Source
DefinitionMicrocosm number (identifier)
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberMicrometerCubedPer0.1Mililiter
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Unitdimensionless
Typereal
UnitnumberPer100Milliliters
Typereal
UnitnumberPer100Milliliters
Typereal
UnitnumberPer100Milliliters
Typereal
UnitnumberPer100Milliliters
Typereal
Unitdimensionless
Typereal
Unitmillimeter
Typereal
UnitnumberPer100Milliliters
Typereal
UnitnumberPer100Milliliters
Typereal
UnitnumberPer100Milliliters
Typereal
UnitnumberPer100Milliliters
Typereal
UnitnumberPer100Milliliters
Typereal
Allowed Values and Definitions
Enumerated Domain 
Code Definition
Code0
DefinitionEphippia is not present
Source
Code Definition
Code1
DefinitionEphippia is present
Source
Code Definition
Code2
DefinitionEphippia is present
Source
Code Definition
Code-99.0000
DefinitionData is not available
Source
UnitnumberPerMilliliter
Typereal
UnitnumberPerMilliliter
Typereal
UnitnumberPerMilliliter
Typereal
UnitnumberPerMilliliter
Typereal
UnitnumberPerMilliliter
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UnitopticalDensity
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UnitmicrogramPerLiter
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UnitmicrogramPerLiter
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UnitmicrogramPerLiter
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UnitmicrogramPerLiter
Typereal
Unitdimensionless
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Missing Value Code:      
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Accuracy Report:                                                                                                                
Accuracy Assessment:                                                                                                                
Coverage:                                                                                                                
Methods:                                                                                                                

Data Table

Data:https://pasta-s.lternet.edu/package/data/eml/edi/1083/1/a1a73e4f584218d31aa9bf27a84fc1f9
Name:ME67
Description:All data for experiment 67 from EPA Duluth Laboratory in Minnesota with six replicates of 0, 500, 1000, and 2000 ppb Cu++.
Number of Records:456
Number of Columns:56

Table Structure
Object Name:ME67.csv
Size:188067 byte
Authentication:aa2fea9b742a919c97910e0b2f8823bb Calculated By MD5
Text Format:
Number of Header Lines:1
Record Delimiter:\r\n
Orientation:column
Simple Delimited:
Field Delimiter:,
Quote Character:"

Table Column Descriptions
 DayTreatmentMicrocosm NumberSelenastrum DensityChlamydomonas DensityScenedesmus DensityAnkistrodesmus DensityChlorella DensityNitzschia Kutzigiana DensityAnabaena Cell DensityLyngbya Cell DesntiyStigeoclonium DensityUlothrix DensityUnidentified Green Alga DensityAvailable AlgaeAlgal BiovolumeDaphnia Fecundity RatioShannon-Weaver Weighted Algal DiversityShannon-Weaver Algal Species DiversitySmall Daphnia ConcentrationMedium Daphnia ConcentrationLarge Daphnia ConcentrationDaphnia ConcentrationSmall to Total Daphnia Concentration RatioDaphnia BiomassSmall Amphipods ConcentrationLarge Amphipods ConcentrationAmphipods ConcentrationOstracods ConcentrationEphippia PresenceRotifer ConcentrationSmall Rotifer ConcentrationHypotrichs ConcentrationMiscellaneous Protozoan ConcentrationVorticella CocentrationNemertines ConcentrationFirst Dissolved Oxygen MeasurementSecond Dissolved Oxygen MeasurementThird Dissolved Oxygen MeasurementDissolved Oxygen GainDissolved Oxygen LossDissolved Oxygen Change Over 24 HoursNet Photosynthesis-Respiration RatioIn vivo FluorescencepH Immediately After Lights OnpH at 1130-1200 HoursPhosphate ConcentrationSilicate ConcentrationNitrate ConcentrationNitrite ConcentrationAmmonia ConcentrationNitrate to Phosphate ratio(Nitrate + Nitrite) to Phosphate RatioTotal Nitrogen to Phosphate Ratio
Column Name:DAY  
TRT  
MIC  
SELAN  
CHLAMY  
SCENED  
ANKIS  
CHLOR  
D216  
ANABENA  
LYNGBYA  
STIG  
ULOTH  
UNKALG1  
AVALGAE  
ABIOVOL  
DFECUND  
DVRSFD  
DVRSALG  
SDAPHNIA  
MDAPHNIA  
LDAPHNIA  
DAPHNIA  
PSDAPHN  
MDSIZE  
DBIOMAS  
SAMPH  
LAMPH  
AMPH  
OSTRACOD  
EPHIPPIA  
PHILODIN  
ROTIFER  
HYPO  
MISCEL  
VORT  
NEMER  
TURBAB  
DO1  
DO2  
DO3  
DDOAM  
DDOPM  
DELDO  
RATIOPR  
FLUOR  
PH1  
PH2  
PO4  
SIO4  
NO3  
NO2  
NH3  
RATIO1  
RATIO2  
RATIO3  
Definition:Day since experiment started the sample was takenThe treatment number assigned to microcosm the data was collected fromMicrocosm number (identifier)Selenastrum abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Chlamydomonas abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Scenedesmus abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Ankistrodesmus abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Chlorella abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Nitzschia Kutzigiana abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Anabaena abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Lyngbya abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Stigeoclonium abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Ulothrix abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Unidentified green alga abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.A measurement of the algae available for Daphnia to feed on based on the biomass of algae and their feeding availability.The measurement of the total algal biovolume of the algae in each microcosm determined by algae count and cell volume.Daphnia Fecundity Ratio. Calculated by SDAPHNIA/LDAPHNIA.An estimate of the diversity of algal cells that are available to the Daphnia for feedingDiversity measurements of all algal species from the Shannon-Weaver equation.Small Daphnia(~0.7mm) count per 100mLMedium Daphnia(0.7mm-1.8mm) count per 100mLLarge Daphnia(>1.8mm) count per 100mLDaphnia count per 100mLThe ratio of small to total daphniaMean Daphnia LengthDaphnia Biomass per 100MLSmall Amphipods(<4mm) per 100 mLLarge Amphipods(>4mm) per 100 mLAmphipods per 100 mLOstracods per 100mLEphippia presence in DaphniaPhilodina acuticornis (rotifer) per mLSmall Rotifers per mLHypotrichs (protozoa) counts per mLMiscellaneous Protozoan per mLVorticella(Protozoa) counts per mLNemertines per mLAbsorbance at 440nM in Spectrophotometer Model 20, which provides a measure of turbidity.First measurement of dissolved oxygen taken in the a.m. before the lights are turned on.Second measurement of dissolved oxygen taken in the p.m. before the lights are turned off.Third measurement of dissolved oxygen taken in the a.m. before the lights are turned on.The amount of gained dissolved oxygen during the light period. This is calculated by subtracting DO1 from DO2.The amount of lost dissolved oxygen during the dark period. This is calculated by subtracting DO3 from DO2.The change in dissolved oxygen over 24 hours. This is calculated by subtracting DO1 from DO3.The ratio of photosynthesis(gain of oxygen) and respiration(loss of oxygen). The equation is as follows: (DO2-DO1)/(DO2-DO3) = (DDOAM)/(DDOPM)In vivo Fluorescence measured in 1-cm diameter tube. Measured using Turner Model #111 fluorometer.pH taken immediately after lights are turned on at 930 hours.pH taken between 1130-1200 hours.Phosphate(PO4) concentrationSilicate(SiO4) concentrationNitrate NO3 ConcentrationNitrite(NO2) ConcentrationAmmonia(NH3) ConcentrationRatio of Nitrate to Phosphate. Calculated by NO3/PO4.Ratio of Nitrate and Nitrite to Phosphate. Calculated by (NO3 + NO2)/PO4.Total Nitrogen to Phosphate Ratio. Calculated by (NO3+NO2+NH3)/PO4.
Storage Type:float  
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float  
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float  
Measurement Type:rationominalnominalratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratiorationominalratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratio
Measurement Values Domain:
UnitnominalDay
Typereal
Allowed Values and Definitions
Enumerated Domain 
Code Definition
Code1
DefinitionControl
Source
Code Definition
Code2
Definition500 ppb Cu^++
Source
Code Definition
Code3
Definition1000 ppb Cu^++
Source
Code Definition
Code4
Definition2000 ppb Cu^++
Source
DefinitionMicrocosm number (identifier)
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
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Typereal
UnitnumberMicrometerCubedPer0.1MilimeterCubed
Typereal
Unitdimensionless
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Unitdimensionless
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UnitnumberPer100Milliliters
Typereal
UnitnumberPer100Milliliters
Typereal
UnitnumberPer100Milliliters
Typereal
UnitnumberPer100Milliliters
Typereal
Unitdimensionless
Typereal
Unitmillimeter
Typereal
UnitnumberPer100Milliliters
Typereal
UnitnumberPer100Milliliters
Typereal
UnitnumberPer100Milliliters
Typereal
UnitnumberPer100Milliliters
Typereal
UnitnumberPer100Milliliters
Typereal
Allowed Values and Definitions
Enumerated Domain 
Code Definition
Code-99
DefinitionData is not available
Source
Code Definition
Code0
DefinitionEphippia is not present
Source
Code Definition
Code1
DefinitionEphippia is present
Source
UnitnumberPerMilliliter
Typereal
UnitnumberPerMilliliter
Typereal
UnitnumberPerMilliliter
Typereal
UnitnumberPerMilliliter
Typereal
UnitnumberPerMilliliter
Typereal
UnitnumberPerMilliliter
Typereal
UnitopticalDensity
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UnitmicrogramPerLiter
Typereal
UnitmicrogramPerLiter
Typereal
UnitmicrogramPerLiter
Typereal
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Unitdimensionless
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Missing Value Code:      
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Accuracy Report:                                                                                                                
Accuracy Assessment:                                                                                                                
Coverage:                                                                                                                
Methods:                                                                                                                

Data Table

Data:https://pasta-s.lternet.edu/package/data/eml/edi/1083/1/53fe7195ea1ad67d8a6a972dacbaa056
Name:ME69
Description:All data for experiment 69 from the University of Washington in Seattle with six replicates of 0, 500, 1000, and 2000 ppb Cu++.
Number of Records:456
Number of Columns:56

Table Structure
Object Name:ME69.csv
Size:110757 byte
Authentication:0ac0cb53a7ff9f44ff4ff4bcbaad6544 Calculated By MD5
Text Format:
Number of Header Lines:1
Record Delimiter:\r\n
Orientation:column
Simple Delimited:
Field Delimiter:,
Quote Character:"

Table Column Descriptions
 DayTreatmentMicrocosm NumberSelenastrum DensityChlamydomonas DensityScenedesmus DensityAnkistrodesmus DensityChlorella DensityNitzschia Kutzigiana DensityAnabaena Cell DensityLyngbya Cell DesntiyStigeoclonium DensityUlothrix DensityUnidentified Green Alga DensityAvailable AlgaeAlgal BiovolumeDaphnia Fecundity RatioShannon-Weaver Weighted Algal DiversityShannon-Weaver Algal Species DiversitySmall Daphnia ConcentrationMedium Daphnia ConcentrationLarge Daphnia ConcentrationDaphnia ConcentrationSmall to Total Daphnia Concentration RatioDaphnia BiomassSmall Amphipods ConcentrationLarge Amphipods ConcentrationAmphipods ConcentrationOstracods ConcentrationEphippia PresenceRotifer ConcentrationSmall Rotifer ConcentrationHypotrichs ConcentrationMiscellaneous Protozoan ConcentrationVorticella CocentrationNemertines ConcentrationFirst Dissolved Oxygen MeasurementSecond Dissolved Oxygen MeasurementThird Dissolved Oxygen MeasurementDissolved Oxygen GainDissolved Oxygen LossDissolved Oxygen Change Over 24 HoursNet Photosynthesis-Respiration RatioIn vivo FluorescencepH Immediately After Lights OnpH at 1130-1200 HoursPhosphate ConcentrationSilicate ConcentrationNitrate ConcentrationNitrite ConcentrationAmmonia ConcentrationNitrate to Phosphate ratio(Nitrate + Nitrite) to Phosphate RatioTotal Nitrogen to Phosphate Ratio
Column Name:DAY  
TRT  
MIC  
SELAN  
CHLAMY  
SCENED  
ANKIS  
CHLOR  
D216  
ANABENA  
LYNGBYA  
STIG  
ULOTH  
UNKALG1  
AVALGAE  
ABIOVOL  
DFECUND  
DVRSFD  
DVRSALG  
SDAPHNIA  
MDAPHNIA  
LDAPHNIA  
DAPHNIA  
PSDAPHN  
MDSIZE  
DBIOMAS  
SAMPH  
LAMPH  
AMPH  
OSTRACOD  
EPHIPPIA  
PHILODIN  
ROTIFER  
HYPO  
MISCEL  
VORT  
NEMER  
TURBAB  
DO1  
DO2  
DO3  
DDOAM  
DDOPM  
DELDO  
RATIOPR  
FLUOR  
PH1  
PH2  
PO4  
SIO4  
NO3  
NO2  
NH3  
RATIO1  
RATIO2  
RATIO3  
Definition:Day since experiment started the sample was takenThe treatment number assigned to microcosm the data was collected fromMicrocosm number (identifier)Selenastrum abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Chlamydomonas abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Scenedesmus abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Ankistrodesmus abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Chlorella abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Nitzschia Kutzigiana abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Anabaena abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Lyngbya abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Stigeoclonium abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Ulothrix abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Unidentified green alga abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.A measurement of the algae available for Daphnia to feed on based on the biomass of algae and their feeding availability.The measurement of the total algal biovolume of the algae in each microcosm determined by algae count and cell volume.Daphnia Fecundity Ratio. Calculated by SDAPHNIA/LDAPHNIA.An estimate of the diversity of algal cells that are available to the Daphnia for feedingDiversity measurements of all algal species from the Shannon-Weaver equation.Small Daphnia(~0.7mm) count per 100mLMedium Daphnia(0.7mm-1.8mm) count per 100mLLarge Daphnia(>1.8mm) count per 100mLDaphnia count per 100mLThe ratio of small to total daphniaMean Daphnia LengthDaphnia Biomass per 100MLSmall Amphipods(<4mm) per 100 mLLarge Amphipods(>4mm) per 100 mLAmphipods per 100 mLOstracods per 100mLEphippia presence in DaphniaPhilodina acuticornis (rotifer) per mLSmall Rotifers per mLHypotrichs (protozoa) counts per mLMiscellaneous Protozoan per mLVorticella(Protozoa) counts per mLNemertines per mLAbsorbance at 440nM in Spectrophotometer Model 20, which provides a measure of turbidity.First measurement of dissolved oxygen taken in the a.m. before the lights are turned on.Second measurement of dissolved oxygen taken in the p.m. before the lights are turned off.Third measurement of dissolved oxygen taken in the a.m. before the lights are turned on.The amount of gained dissolved oxygen during the light period. This is calculated by subtracting DO1 from DO2.The amount of lost dissolved oxygen during the dark period. This is calculated by subtracting DO3 from DO2.The change in dissolved oxygen over 24 hours. This is calculated by subtracting DO1 from DO3.The ratio of photosynthesis(gain of oxygen) and respiration(loss of oxygen). The equation is as follows: (DO2-DO1)/(DO2-DO3) = (DDOAM)/(DDOPM)In vivo Fluorescence measured in 1-cm diameter tube. Measured using Turner Model #111 fluorometer.pH taken immediately after lights are turned on at 930 hours.pH taken between 1130-1200 hours.Phosphate(PO4) concentrationSilicate(SiO4) concentrationNitrate NO3 ConcentrationNitrite(NO2) ConcentrationAmmonia(NH3) ConcentrationRatio of Nitrate to Phosphate. Calculated by NO3/PO4.Ratio of Nitrate and Nitrite to Phosphate. Calculated by (NO3 + NO2)/PO4.Total Nitrogen to Phosphate Ratio. Calculated by (NO3+NO2+NH3)/PO4.
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Measurement Type:rationominalnominalratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratiorationominalratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratio
Measurement Values Domain:
UnitnominalDay
Typereal
Allowed Values and Definitions
Enumerated Domain 
Code Definition
Code1
DefinitionControl
Source
Code Definition
Code2
Definition500 ppb Cu^++
Source
Code Definition
Code3
Definition1000 ppb Cu^++
Source
Code Definition
Code4
Definition2000 ppb Cu^++
Source
DefinitionMicrocosm number (identifier)
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
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UnitnumberPer100Milliliters
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Code-99
DefinitionData is not available
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Code Definition
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DefinitionEphippia is not present
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Code Definition
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Expldata is not available
Code-99.0000
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Code-99.0000
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Code-99.0000
Expldata is not available
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Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Accuracy Report:                                                                                                                
Accuracy Assessment:                                                                                                                
Coverage:                                                                                                                
Methods:                                                                                                                

Data Table

Data:https://pasta-s.lternet.edu/package/data/eml/edi/1083/1/274ed9897cb1c8a9313d524a74556408
Name:ME72
Description:All data for experiment 72 from the University of Washington in Seattle with six replicates of 0, 500, 1000, and 2000 ppb Cu++.
Number of Records:456
Number of Columns:56

Table Structure
Object Name:ME72.csv
Size:112239 byte
Authentication:f91cbe9b46bcc44cce4bbc8c3351d9fd Calculated By MD5
Text Format:
Number of Header Lines:1
Record Delimiter:\r\n
Orientation:column
Simple Delimited:
Field Delimiter:,
Quote Character:"

Table Column Descriptions
 DayTreatmentMicrocosm NumberSelenastrum DensityChlamydomonas DensityScenedesmus DensityAnkistrodesmus DensityChlorella DensityNitzschia Kutzigiana DensityAnabaena Cell DensityLyngbya Cell DesntiyStigeoclonium DensityUlothrix DensityUnidentified Green Alga DensityAvailable AlgaeAlgal BiovolumeDaphnia Fecundity RatioShannon-Weaver Weighted Algal DiversityShannon-Weaver Algal Species DiversitySmall Daphnia ConcentrationMedium Daphnia ConcentrationLarge Daphnia ConcentrationDaphnia ConcentrationSmall to Total Daphnia Concentration RatioDaphnia BiomassSmall Amphipods ConcentrationLarge Amphipods ConcentrationAmphipods ConcentrationOstracods ConcentrationEphippia PresenceRotifer ConcentrationSmall Rotifer ConcentrationHypotrichs ConcentrationMiscellaneous Protozoan ConcentrationVorticella CocentrationNemertines ConcentrationFirst Dissolved Oxygen MeasurementSecond Dissolved Oxygen MeasurementThird Dissolved Oxygen MeasurementDissolved Oxygen GainDissolved Oxygen LossDissolved Oxygen Change Over 24 HoursNet Photosynthesis-Respiration RatioIn vivo FluorescencepH Immediately After Lights OnpH at 1130-1200 HoursPhosphate ConcentrationSilicate ConcentrationNitrate ConcentrationNitrite ConcentrationAmmonia ConcentrationNitrate to Phosphate ratio(Nitrate + Nitrite) to Phosphate RatioTotal Nitrogen to Phosphate Ratio
Column Name:DAY  
TRT  
MIC  
SELAN  
CHLAMY  
SCENED  
ANKIS  
CHLOR  
D216  
ANABENA  
LYNGBYA  
STIG  
ULOTH  
UNKALG1  
AVALGAE  
ABIOVOL  
DFECUND  
DVRSFD  
DVRSALG  
SDAPHNIA  
MDAPHNIA  
LDAPHNIA  
DAPHNIA  
PSDAPHN  
MDSIZE  
DBIOMAS  
SAMPH  
LAMPH  
AMPH  
OSTRACOD  
EPHIPPIA  
PHILODIN  
ROTIFER  
HYPO  
MISCEL  
VORT  
NEMER  
TURBAB  
DO1  
DO2  
DO3  
DDOAM  
DDOPM  
DELDO  
RATIOPR  
FLUOR  
PH1  
PH2  
PO4  
SIO4  
NO3  
NO2  
NH3  
RATIO1  
RATIO2  
RATIO3  
Definition:Day since experiment started the sample was takenThe treatment number assigned to microcosm the data was collected fromMicrocosm number (identifier)Selenastrum abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Chlamydomonas abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Scenedesmus abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Ankistrodesmus abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Chlorella abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Nitzschia Kutzigiana abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Anabaena abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Lyngbya abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Stigeoclonium abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Ulothrix abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Unidentified green alga abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.A measurement of the algae available for Daphnia to feed on based on the biomass of algae and their feeding availability.The measurement of the total algal biovolume of the algae in each microcosm determined by algae count and cell volume.Daphnia Fecundity Ratio. Calculated by SDAPHNIA/LDAPHNIA.An estimate of the diversity of algal cells that are available to the Daphnia for feedingDiversity measurements of all algal species from the Shannon-Weaver equation.Small Daphnia(~0.7mm) count per 100mLMedium Daphnia(0.7mm-1.8mm) count per 100mLLarge Daphnia(>1.8mm) count per 100mLDaphnia count per 100mLThe ratio of small to total daphniaMean Daphnia LengthDaphnia Biomass per 100MLSmall Amphipods(<4mm) per 100 mLLarge Amphipods(>4mm) per 100 mLAmphipods per 100 mLOstracods per 100mLEphippia presence in DaphniaPhilodina acuticornis (rotifer) per mLSmall Rotifers per mLHypotrichs (protozoa) counts per mLMiscellaneous Protozoan per mLVorticella(Protozoa) counts per mLNemertines per mLAbsorbance at 440nM in Spectrophotometer Model 20, which provides a measure of turbidity.First measurement of dissolved oxygen taken in the a.m. before the lights are turned on.Second measurement of dissolved oxygen taken in the p.m. before the lights are turned off.Third measurement of dissolved oxygen taken in the a.m. before the lights are turned on.The amount of gained dissolved oxygen during the light period. This is calculated by subtracting DO1 from DO2.The amount of lost dissolved oxygen during the dark period. This is calculated by subtracting DO3 from DO2.The change in dissolved oxygen over 24 hours. This is calculated by subtracting DO1 from DO3.The ratio of photosynthesis(gain of oxygen) and respiration(loss of oxygen). The equation is as follows: (DO2-DO1)/(DO2-DO3) = (DDOAM)/(DDOPM)In vivo Fluorescence measured in 1-cm diameter tube. Measured using Turner Model #111 fluorometer.pH taken immediately after lights are turned on at 930 hours.pH taken between 1130-1200 hours.Phosphate(PO4) concentrationSilicate(SiO4) concentrationNitrate NO3 ConcentrationNitrite(NO2) ConcentrationAmmonia(NH3) ConcentrationRatio of Nitrate to Phosphate. Calculated by NO3/PO4.Ratio of Nitrate and Nitrite to Phosphate. Calculated by (NO3 + NO2)/PO4.Total Nitrogen to Phosphate Ratio. Calculated by (NO3+NO2+NH3)/PO4.
Storage Type:float  
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Measurement Type:rationominalnominalratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratiorationominalratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratio
Measurement Values Domain:
UnitnominalDay
Typereal
Allowed Values and Definitions
Enumerated Domain 
Code Definition
Code1
DefinitionControl
Source
Code Definition
Code2
Definition500 ppb Cu^++
Source
Code Definition
Code3
Definition1000 ppb Cu^++
Source
Code Definition
Code4
Definition2000 ppb Cu^++
Source
DefinitionMicrocosm number (identifier)
UnitnumberPer0.1MillimeterCubed
Typereal
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Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
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UnitnumberPer100Milliliters
Typereal
UnitnumberPer100Milliliters
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UnitnumberPer100Milliliters
Typereal
Allowed Values and Definitions
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Code Definition
Code-99
DefinitionData is not available
Source
Code Definition
Code0
DefinitionEphippia is not present
Source
Code Definition
Code1
DefinitionEphippia is present
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Code-99.0000
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Code-99.0000
Expldata is not available
Code-99.0000
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Expldata is not available
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Expldata is not available
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Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
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Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
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Expldata is not available
Code-99.0000
Expldata is not available
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Expldata is not available
Code-99.0000
Expldata is not available
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Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Accuracy Report:                                                                                                                
Accuracy Assessment:                                                                                                                
Coverage:                                                                                                                
Methods:                                                                                                                

Data Table

Data:https://pasta-s.lternet.edu/package/data/eml/edi/1083/1/3f106bbc35354d54e9f0cbfc9f058788
Name:ME74
Description:All data for experiment 74 from the Aberdeen-Army lab in Maryland with six replicates of 0, 127, 255, and 509 ppb Cu++.
Number of Records:456
Number of Columns:56

Table Structure
Object Name:ME74.csv
Size:114888 byte
Authentication:ba8f0b99e5c775c923376e6c6f317ecf Calculated By MD5
Text Format:
Number of Header Lines:1
Record Delimiter:\r\n
Orientation:column
Simple Delimited:
Field Delimiter:,
Quote Character:"

Table Column Descriptions
 DayTreatmentMicrocosm NumberSelenastrum DensityChlamydomonas DensityScenedesmus DensityAnkistrodesmus DensityChlorella DensityNitzschia Kutzigiana DensityAnabaena Cell DensityLyngbya Cell DesntiyStigeoclonium DensityUlothrix DensityUnidentified Green Alga DensityAvailable AlgaeAlgal BiovolumeDaphnia Fecundity RatioShannon-Weaver Weighted Algal DiversityShannon-Weaver Algal Species DiversitySmall Daphnia ConcentrationMedium Daphnia ConcentrationLarge Daphnia ConcentrationDaphnia ConcentrationSmall to Total Daphnia Concentration RatioDaphnia BiomassSmall Amphipods ConcentrationLarge Amphipods ConcentrationAmphipods ConcentrationOstracods ConcentrationEphippia PresenceRotifer ConcentrationSmall Rotifer ConcentrationHypotrichs ConcentrationMiscellaneous Protozoan ConcentrationVorticella CocentrationNemertines ConcentrationFirst Dissolved Oxygen MeasurementSecond Dissolved Oxygen MeasurementThird Dissolved Oxygen MeasurementDissolved Oxygen GainDissolved Oxygen LossDissolved Oxygen Change Over 24 HoursNet Photosynthesis-Respiration RatioIn vivo FluorescencepH Immediately After Lights OnpH at 1130-1200 HoursPhosphate ConcentrationSilicate ConcentrationNitrate ConcentrationNitrite ConcentrationAmmonia ConcentrationNitrate to Phosphate ratio(Nitrate + Nitrite) to Phosphate RatioTotal Nitrogen to Phosphate Ratio
Column Name:DAY  
TRT  
MIC  
SELAN  
CHLAMY  
SCENED  
ANKIS  
CHLOR  
D216  
ANABENA  
LYNGBYA  
STIG  
ULOTH  
UNKALG1  
AVALGAE  
ABIOVOL  
DFECUND  
DVRSFD  
DVRSALG  
SDAPHNIA  
MDAPHNIA  
LDAPHNIA  
DAPHNIA  
PSDAPHN  
MDSIZE  
DBIOMAS  
SAMPH  
LAMPH  
AMPH  
OSTRACOD  
EPHIPPIA  
PHILODIN  
ROTIFER  
HYPO  
MISCEL  
VORT  
NEMER  
TURBAB  
DO1  
DO2  
DO3  
DDOAM  
DDOPM  
DELDO  
RATIOPR  
FLUOR  
PH1  
PH2  
PO4  
SIO4  
NO3  
NO2  
NH3  
RATIO1  
RATIO2  
RATIO3  
Definition:Day since experiment started the sample was takenThe treatment number assigned to microcosm the data was collected fromMicrocosm number (identifier)Selenastrum abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Chlamydomonas abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Scenedesmus abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Ankistrodesmus abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Chlorella abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Nitzschia Kutzigiana abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Anabaena abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Lyngbya abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Stigeoclonium abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Ulothrix abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Unidentified green alga abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.A measurement of the algae available for Daphnia to feed on based on the biomass of algae and their feeding availability.The measurement of the total algal biovolume of the algae in each microcosm determined by algae count and cell volume.Daphnia Fecundity Ratio. Calculated by SDAPHNIA/LDAPHNIA.An estimate of the diversity of algal cells that are available to the Daphnia for feedingDiversity measurements of all algal species from the Shannon-Weaver equation.Small Daphnia(~0.7mm) count per 100mLMedium Daphnia(0.7mm-1.8mm) count per 100mLLarge Daphnia(>1.8mm) count per 100mLDaphnia count per 100mLThe ratio of small to total daphniaMean Daphnia LengthDaphnia Biomass per 100MLSmall Amphipods(<4mm) per 100 mLLarge Amphipods(>4mm) per 100 mLAmphipods per 100 mLOstracods per 100mLEphippia presence in DaphniaPhilodina acuticornis (rotifer) per mLSmall Rotifers per mLHypotrichs (protozoa) counts per mLMiscellaneous Protozoan per mLVorticella(Protozoa) counts per mLNemertines per mLAbsorbance at 440nM in Spectrophotometer Model 20, which provides a measure of turbidity.First measurement of dissolved oxygen taken in the a.m. before the lights are turned on.Second measurement of dissolved oxygen taken in the p.m. before the lights are turned off.Third measurement of dissolved oxygen taken in the a.m. before the lights are turned on.The amount of gained dissolved oxygen during the light period. This is calculated by subtracting DO1 from DO2.The amount of lost dissolved oxygen during the dark period. This is calculated by subtracting DO3 from DO2.The change in dissolved oxygen over 24 hours. This is calculated by subtracting DO1 from DO3.The ratio of photosynthesis(gain of oxygen) and respiration(loss of oxygen). The equation is as follows: (DO2-DO1)/(DO2-DO3) = (DDOAM)/(DDOPM)In vivo Fluorescence measured in 1-cm diameter tube. Measured using Turner Model #111 fluorometer.pH taken immediately after lights are turned on at 930 hours.pH taken between 1130-1200 hours.Phosphate(PO4) concentrationSilicate(SiO4) concentrationNitrate NO3 ConcentrationNitrite(NO2) ConcentrationAmmonia(NH3) ConcentrationRatio of Nitrate to Phosphate. Calculated by NO3/PO4.Ratio of Nitrate and Nitrite to Phosphate. Calculated by (NO3 + NO2)/PO4.Total Nitrogen to Phosphate Ratio. Calculated by (NO3+NO2+NH3)/PO4.
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float  
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Measurement Type:rationominalnominalratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratiorationominalratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratio
Measurement Values Domain:
UnitnominalDay
Typereal
Allowed Values and Definitions
Enumerated Domain 
Code Definition
Code1
DefinitionControl
Source
Code Definition
Code2
Definition500 ppb Cu^++
Source
Code Definition
Code3
Definition1000 ppb Cu^++
Source
Code Definition
Code4
Definition2000 ppb Cu^++
Source
DefinitionMicrocosm number (identifier)
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
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UnitnumberPer0.1MillimeterCubed
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UnitnumberPer0.1MillimeterCubed
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UnitnumberPer0.1MillimeterCubed
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UnitnumberPer100Milliliters
Typereal
UnitnumberPer100Milliliters
Typereal
UnitnumberPer100Milliliters
Typereal
UnitnumberPer100Milliliters
Typereal
UnitnumberPer100Milliliters
Typereal
Allowed Values and Definitions
Enumerated Domain 
Code Definition
Code-99
DefinitionData is not available
Source
Code Definition
Code0
DefinitionEphippia is not present
Source
Code Definition
Code1
DefinitionEphippia is present
Source
UnitnumberPerMilliliter
Typereal
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Missing Value Code:      
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
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Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
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Expldata is not available
Code-99.0000
Expldata is not available
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Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Accuracy Report:                                                                                                                
Accuracy Assessment:                                                                                                                
Coverage:                                                                                                                
Methods:                                                                                                                

Data Table

Data:https://pasta-s.lternet.edu/package/data/eml/edi/1083/1/02d7190c706895131297a1e9484985e9
Name:ME79
Description:All data for experiment 79 from the Marine Bioassay Laboratories, Inc. in California with six replicates of 0, 500, 1000, and 2000 ppb Cu++.
Number of Records:456
Number of Columns:56

Table Structure
Object Name:ME79.csv
Size:112489 byte
Authentication:5517568e278e67780848dc8411503056 Calculated By MD5
Text Format:
Number of Header Lines:1
Record Delimiter:\r\n
Orientation:column
Simple Delimited:
Field Delimiter:,
Quote Character:"

Table Column Descriptions
 DayTreatmentMicrocosm NumberSelenastrum DensityChlamydomonas DensityScenedesmus DensityAnkistrodesmus DensityChlorella DensityNitzschia Kutzigiana DensityAnabaena Cell DensityLyngbya Cell DesntiyStigeoclonium DensityUlothrix DensityUnidentified Green Alga DensityAvailable AlgaeAlgal BiovolumeDaphnia Fecundity RatioShannon-Weaver Weighted Algal DiversityShannon-Weaver Algal Species DiversitySmall Daphnia ConcentrationMedium Daphnia ConcentrationLarge Daphnia ConcentrationDaphnia ConcentrationSmall to Total Daphnia Concentration RatioDaphnia BiomassSmall Amphipods ConcentrationLarge Amphipods ConcentrationAmphipods ConcentrationOstracods ConcentrationEphippia PresenceRotifer ConcentrationSmall Rotifer ConcentrationHypotrichs ConcentrationMiscellaneous Protozoan ConcentrationVorticella CocentrationNemertines ConcentrationFirst Dissolved Oxygen MeasurementSecond Dissolved Oxygen MeasurementThird Dissolved Oxygen MeasurementDissolved Oxygen GainDissolved Oxygen LossDissolved Oxygen Change Over 24 HoursNet Photosynthesis-Respiration RatioIn vivo FluorescencepH Immediately After Lights OnpH at 1130-1200 HoursPhosphate ConcentrationSilicate ConcentrationNitrate ConcentrationNitrite ConcentrationAmmonia ConcentrationNitrate to Phosphate ratio(Nitrate + Nitrite) to Phosphate RatioTotal Nitrogen to Phosphate Ratio
Column Name:DAY  
TRT  
MIC  
SELAN  
CHLAMY  
SCENED  
ANKIS  
CHLOR  
D216  
ANABENA  
LYNGBYA  
STIG  
ULOTH  
UNKALG1  
AVALGAE  
ABIOVOL  
DFECUND  
DVRSFD  
DVRSALG  
SDAPHNIA  
MDAPHNIA  
LDAPHNIA  
DAPHNIA  
PSDAPHN  
MDSIZE  
DBIOMAS  
SAMPH  
LAMPH  
AMPH  
OSTRACOD  
EPHIPPIA  
PHILODIN  
ROTIFER  
HYPO  
MISCEL  
VORT  
NEMER  
TURBAB  
DO1  
DO2  
DO3  
DDOAM  
DDOPM  
DELDO  
RATIOPR  
FLUOR  
PH1  
PH2  
PO4  
SIO4  
NO3  
NO2  
NH3  
RATIO1  
RATIO2  
RATIO3  
Definition:Day since experiment started the sample was takenThe treatment number assigned to microcosm the data was collected fromMicrocosm number (identifier)Selenastrum abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Chlamydomonas abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Scenedesmus abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Ankistrodesmus abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Chlorella abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Nitzschia Kutzigiana abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Anabaena abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Lyngbya abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Stigeoclonium abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Ulothrix abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Unidentified green alga abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.A measurement of the algae available for Daphnia to feed on based on the biomass of algae and their feeding availability.The measurement of the total algal biovolume of the algae in each microcosm determined by algae count and cell volume.Daphnia Fecundity Ratio. Calculated by SDAPHNIA/LDAPHNIA.An estimate of the diversity of algal cells that are available to the Daphnia for feedingDiversity measurements of all algal species from the Shannon-Weaver equation.Small Daphnia(~0.7mm) count per 100mLMedium Daphnia(0.7mm-1.8mm) count per 100mLLarge Daphnia(>1.8mm) count per 100mLDaphnia count per 100mLThe ratio of small to total daphniaMean Daphnia LengthDaphnia Biomass per 100MLSmall Amphipods(<4mm) per 100 mLLarge Amphipods(>4mm) per 100 mLAmphipods per 100 mLOstracods per 100mLEphippia presence in DaphniaPhilodina acuticornis (rotifer) per mLSmall Rotifers per mLHypotrichs (protozoa) counts per mLMiscellaneous Protozoan per mLVorticella(Protozoa) counts per mLNemertines per mLAbsorbance at 440nM in Spectrophotometer Model 20, which provides a measure of turbidity.First measurement of dissolved oxygen taken in the a.m. before the lights are turned on.Second measurement of dissolved oxygen taken in the p.m. before the lights are turned off.Third measurement of dissolved oxygen taken in the a.m. before the lights are turned on.The amount of gained dissolved oxygen during the light period. This is calculated by subtracting DO1 from DO2.The amount of lost dissolved oxygen during the dark period. This is calculated by subtracting DO3 from DO2.The change in dissolved oxygen over 24 hours. This is calculated by subtracting DO1 from DO3.The ratio of photosynthesis(gain of oxygen) and respiration(loss of oxygen). The equation is as follows: (DO2-DO1)/(DO2-DO3) = (DDOAM)/(DDOPM)In vivo Fluorescence measured in 1-cm diameter tube. Measured using Turner Model #111 fluorometer.pH taken immediately after lights are turned on at 930 hours.pH taken between 1130-1200 hours.Phosphate(PO4) concentrationSilicate(SiO4) concentrationNitrate NO3 ConcentrationNitrite(NO2) ConcentrationAmmonia(NH3) ConcentrationRatio of Nitrate to Phosphate. Calculated by NO3/PO4.Ratio of Nitrate and Nitrite to Phosphate. Calculated by (NO3 + NO2)/PO4.Total Nitrogen to Phosphate Ratio. Calculated by (NO3+NO2+NH3)/PO4.
Storage Type:float  
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Measurement Type:rationominalnominalratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratiorationominalratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratio
Measurement Values Domain:
UnitnominalDay
Typereal
Allowed Values and Definitions
Enumerated Domain 
Code Definition
Code1
DefinitionControl
Source
Code Definition
Code2
Definition500 ppb Cu^++
Source
Code Definition
Code3
Definition1000 ppb Cu^++
Source
Code Definition
Code4
Definition2000 ppb Cu^++
Source
DefinitionMicrocosm number (identifier)
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
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UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
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UnitnumberPer0.1MillimeterCubed
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UnitnumberPer0.1MillimeterCubed
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UnitnumberPer0.1MillimeterCubed
Typereal
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UnitnumberPer100Milliliters
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Typereal
UnitnumberPer100Milliliters
Typereal
UnitnumberPer100Milliliters
Typereal
UnitnumberPer100Milliliters
Typereal
UnitnumberPer100Milliliters
Typereal
Allowed Values and Definitions
Enumerated Domain 
Code Definition
Code-99
DefinitionData is not available
Source
Code Definition
Code0
DefinitionEphippia is not present
Source
Code Definition
Code1
DefinitionEphippia is present
Source
Code Definition
Code2
DefinitionEphippia is present
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UnitnumberPerMilliliter
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Missing Value Code:      
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
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Expldata is not available
Code-99.0000
Expldata is not available
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Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
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Expldata is not available
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Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Accuracy Report:                                                                                                                
Accuracy Assessment:                                                                                                                
Coverage:                                                                                                                
Methods:                                                                                                                

Data Table

Data:https://pasta-s.lternet.edu/package/data/eml/edi/1083/1/3851e5043908421e2c89b9c05e656f79
Name:ME82
Description:All data for experiment 82 from the University of Washington in Seattle with six replicates of 0, 500, 1000, and 2000 ppb Cu++.
Number of Records:456
Number of Columns:56

Table Structure
Object Name:ME82.csv
Size:113118 byte
Authentication:9ba37d5c553661751ab6bb4b6f85fc54 Calculated By MD5
Text Format:
Number of Header Lines:1
Record Delimiter:\r\n
Orientation:column
Simple Delimited:
Field Delimiter:,
Quote Character:"

Table Column Descriptions
 DayTreatmentMicrocosm NumberSelenastrum DensityChlamydomonas DensityScenedesmus DensityAnkistrodesmus DensityChlorella DensityNitzschia Kutzigiana DensityAnabaena Cell DensityLyngbya Cell DesntiyStigeoclonium DensityUlothrix DensityUnidentified Green Alga DensityAvailable AlgaeAlgal BiovolumeDaphnia Fecundity RatioShannon-Weaver Weighted Algal DiversityShannon-Weaver Algal Species DiversitySmall Daphnia ConcentrationMedium Daphnia ConcentrationLarge Daphnia ConcentrationDaphnia ConcentrationSmall to Total Daphnia Concentration RatioDaphnia BiomassSmall Amphipods ConcentrationLarge Amphipods ConcentrationAmphipods ConcentrationOstracods ConcentrationEphippia PresenceRotifer ConcentrationSmall Rotifer ConcentrationHypotrichs ConcentrationMiscellaneous Protozoan ConcentrationVorticella CocentrationNemertines ConcentrationFirst Dissolved Oxygen MeasurementSecond Dissolved Oxygen MeasurementThird Dissolved Oxygen MeasurementDissolved Oxygen GainDissolved Oxygen LossDissolved Oxygen Change Over 24 HoursNet Photosynthesis-Respiration RatioIn vivo FluorescencepH Immediately After Lights OnpH at 1130-1200 HoursPhosphate ConcentrationSilicate ConcentrationNitrate ConcentrationNitrite ConcentrationAmmonia ConcentrationNitrate to Phosphate ratio(Nitrate + Nitrite) to Phosphate RatioTotal Nitrogen to Phosphate Ratio
Column Name:DAY  
TRT  
MIC  
SELAN  
CHLAMY  
SCENED  
ANKIS  
CHLOR  
D216  
ANABENA  
LYNGBYA  
STIG  
ULOTH  
UNKALG1  
AVALGAE  
ABIOVOL  
DFECUND  
DVRSFD  
DVRSALG  
SDAPHNIA  
MDAPHNIA  
LDAPHNIA  
DAPHNIA  
PSDAPHN  
MDSIZE  
DBIOMAS  
SAMPH  
LAMPH  
AMPH  
OSTRACOD  
EPHIPPIA  
PHILODIN  
ROTIFER  
HYPO  
MISCEL  
VORT  
NEMER  
TURBAB  
DO1  
DO2  
DO3  
DDOAM  
DDOPM  
DELDO  
RATIOPR  
FLUOR  
PH1  
PH2  
PO4  
SIO4  
NO3  
NO2  
NH3  
RATIO1  
RATIO2  
RATIO3  
Definition:Day since experiment started the sample was takenThe treatment number assigned to microcosm the data was collected fromMicrocosm number (identifier)Selenastrum abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Chlamydomonas abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Scenedesmus abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Ankistrodesmus abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Chlorella abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Nitzschia Kutzigiana abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Anabaena abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Lyngbya abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Stigeoclonium abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Ulothrix abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Unidentified green alga abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.A measurement of the algae available for Daphnia to feed on based on the biomass of algae and their feeding availability.The measurement of the total algal biovolume of the algae in each microcosm determined by algae count and cell volume.Daphnia Fecundity Ratio. Calculated by SDAPHNIA/LDAPHNIA.An estimate of the diversity of algal cells that are available to the Daphnia for feedingDiversity measurements of all algal species from the Shannon-Weaver equation.Small Daphnia(~0.7mm) count per 100mLMedium Daphnia(0.7mm-1.8mm) count per 100mLLarge Daphnia(>1.8mm) count per 100mLDaphnia count per 100mLThe ratio of small to total daphniaMean Daphnia LengthDaphnia Biomass per 100MLSmall Amphipods(<4mm) per 100 mLLarge Amphipods(>4mm) per 100 mLAmphipods per 100 mLOstracods per 100mLEphippia presence in DaphniaPhilodina acuticornis (rotifer) per mLSmall Rotifers per mLHypotrichs (protozoa) counts per mLMiscellaneous Protozoan per mLVorticella(Protozoa) counts per mLNemertines per mLAbsorbance at 440nM in Spectrophotometer Model 20, which provides a measure of turbidity.First measurement of dissolved oxygen taken in the a.m. before the lights are turned on.Second measurement of dissolved oxygen taken in the p.m. before the lights are turned off.Third measurement of dissolved oxygen taken in the a.m. before the lights are turned on.The amount of gained dissolved oxygen during the light period. This is calculated by subtracting DO1 from DO2.The amount of lost dissolved oxygen during the dark period. This is calculated by subtracting DO3 from DO2.The change in dissolved oxygen over 24 hours. This is calculated by subtracting DO1 from DO3.The ratio of photosynthesis(gain of oxygen) and respiration(loss of oxygen). The equation is as follows: (DO2-DO1)/(DO2-DO3) = (DDOAM)/(DDOPM)In vivo Fluorescence measured in 1-cm diameter tube. Measured using Turner Model #111 fluorometer.pH taken immediately after lights are turned on at 930 hours.pH taken between 1130-1200 hours.Phosphate(PO4) concentrationSilicate(SiO4) concentrationNitrate NO3 ConcentrationNitrite(NO2) ConcentrationAmmonia(NH3) ConcentrationRatio of Nitrate to Phosphate. Calculated by NO3/PO4.Ratio of Nitrate and Nitrite to Phosphate. Calculated by (NO3 + NO2)/PO4.Total Nitrogen to Phosphate Ratio. Calculated by (NO3+NO2+NH3)/PO4.
Storage Type:float  
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float  
Measurement Type:rationominalnominalratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratiorationominalratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratio
Measurement Values Domain:
UnitnominalDay
Typereal
Allowed Values and Definitions
Enumerated Domain 
Code Definition
Code1
DefinitionControl
Source
Code Definition
Code2
Definition500 ppb Cu^++
Source
Code Definition
Code3
Definition1000 ppb Cu^++
Source
Code Definition
Code4
Definition2000 ppb Cu^++
Source
DefinitionMicrocosm number (identifier)
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberMicrometerCubedPer0.1Mililiter
Typereal
UnitnumberMicrometerCubedPer0.1MilimeterCubed
Typereal
Unitdimensionless
Typereal
Unitdimensionless
Typereal
Unitdimensionless
Typereal
UnitnumberPer100Milliliters
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UnitnumberPer100Milliliters
Typereal
UnitnumberPer100Milliliters
Typereal
UnitnumberPer100Milliliters
Typereal
Unitdimensionless
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Unitmillimeter
Typereal
UnitnumberPer100Milliliters
Typereal
UnitnumberPer100Milliliters
Typereal
UnitnumberPer100Milliliters
Typereal
UnitnumberPer100Milliliters
Typereal
UnitnumberPer100Milliliters
Typereal
Allowed Values and Definitions
Enumerated Domain 
Code Definition
Code-99
DefinitionData is not available
Source
Code Definition
Code0
DefinitionEphippia is not present
Source
Code Definition
Code1
DefinitionEphippia is present
Source
UnitnumberPerMilliliter
Typereal
UnitnumberPerMilliliter
Typereal
UnitnumberPerMilliliter
Typereal
UnitnumberPerMilliliter
Typereal
UnitnumberPerMilliliter
Typereal
UnitnumberPerMilliliter
Typereal
UnitopticalDensity
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UnitpartsPerMillion
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UnitpartsPerMillion
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UnitpartsPerMillion
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UnitpartsPerMillion
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UnitpartsPerMillion
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UnitpartsPerMillion
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Unitdimensionless
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Unitdimensionless
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UnitmicrogramPerLiter
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UnitmicrogramPerLiter
Typereal
UnitmicrogramPerLiter
Typereal
UnitmicrogramPerLiter
Typereal
Unitdimensionless
Typereal
Unitdimensionless
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Unitdimensionless
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Missing Value Code:      
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Accuracy Report:                                                                                                                
Accuracy Assessment:                                                                                                                
Coverage:                                                                                                                
Methods:                                                                                                                

Data Table

Data:https://pasta-s.lternet.edu/package/data/eml/edi/1083/1/bd81d0a1668c6281c9019fa7ec669474
Name:ME87
Description:All data for experiment 87 from the Army Aberdeen lab in Maryland with six replicates of 0, 500, 1000, and 2000 ppb Cu++. This experiment does not contain nutrient data.
Number of Records:432
Number of Columns:48

Table Structure
Object Name:ME87.csv
Size:85952 byte
Authentication:cefcd5a75fe9cbe9bde2ea305f07bdd3 Calculated By MD5
Text Format:
Number of Header Lines:1
Record Delimiter:\r\n
Orientation:column
Simple Delimited:
Field Delimiter:,
Quote Character:"

Table Column Descriptions
 DayTreatmentMicrocosm NumberSelenastrum DensityChlamydomonas DensityScenedesmus DensityAnkistrodesmus DensityChlorella DensityNitzschia Kutzigiana DensityAnabaena Cell DensityLyngbya Cell DesntiyStigeoclonium DensityUlothrix DensityUnidentified Green Alga DensityAvailable AlgaeAlgal BiovolumeDaphnia Fecundity RatioShannon-Weaver Weighted Algal DiversityShannon-Weaver Algal Species DiversitySmall Daphnia ConcentrationMedium Daphnia ConcentrationLarge Daphnia ConcentrationDaphnia ConcentrationSmall to Total Daphnia Concentration RatioDaphnia BiomassSmall Amphipods ConcentrationLarge Amphipods ConcentrationAmphipods ConcentrationOstracods ConcentrationEphippia PresenceRotifer ConcentrationSmall Rotifer ConcentrationHypotrichs ConcentrationMiscellaneous Protozoan ConcentrationVorticella CocentrationNemertines ConcentrationFirst Dissolved Oxygen MeasurementSecond Dissolved Oxygen MeasurementThird Dissolved Oxygen MeasurementDissolved Oxygen GainDissolved Oxygen LossDissolved Oxygen Change Over 24 HoursNet Photosynthesis-Respiration RatioIn vivo FluorescencepH Immediately After Lights OnpH at 1130-1200 Hours
Column Name:DAY  
TRT  
MIC  
SELAN  
CHLAMY  
SCENED  
ANKIS  
CHLOR  
D216  
ANABENA  
LYNGBYA  
STIG  
ULOTH  
UNKALG1  
AVALGAE  
ABIOVOL  
DFECUND  
DVRSFD  
DVRSALG  
SDAPHNIA  
MDAPHNIA  
LDAPHNIA  
DAPHNIA  
PSDAPHN  
MDSIZE  
DBIOMAS  
SAMPH  
LAMPH  
AMPH  
OSTRACOD  
EPHIPPIA  
PHILODIN  
ROTIFER  
HYPO  
MISCEL  
VORT  
NEMER  
TURBAB  
DO1  
DO2  
DO3  
DDOAM  
DDOPM  
DELDO  
RATIOPR  
FLUOR  
PH1  
PH2  
Definition:Day since experiment started the sample was takenThe treatment number assigned to microcosm the data was collected fromMicrocosm number (identifier)Selenastrum abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Chlamydomonas abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Scenedesmus abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Ankistrodesmus abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Chlorella abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Nitzschia Kutzigiana abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Anabaena abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Lyngbya abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Stigeoclonium abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Ulothrix abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Unidentified green alga abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.A measurement of the algae available for Daphnia to feed on based on the biomass of algae and their feeding availability.The measurement of the total algal biovolume of the algae in each microcosm determined by algae count and cell volume.Daphnia Fecundity Ratio. Calculated by SDAPHNIA/LDAPHNIA.An estimate of the diversity of algal cells that are available to the Daphnia for feedingDiversity measurements of all algal species from the Shannon-Weaver equation.Small Daphnia(~0.7mm) count per 100mLMedium Daphnia(0.7mm-1.8mm) count per 100mLLarge Daphnia(>1.8mm) count per 100mLDaphnia count per 100mLThe ratio of small to total daphniaMean Daphnia LengthDaphnia Biomass per 100MLSmall Amphipods(<4mm) per 100 mLLarge Amphipods(>4mm) per 100 mLAmphipods per 100 mLOstracods per 100mLEphippia presence in DaphniaPhilodina acuticornis (rotifer) per mLSmall Rotifers per mLHypotrichs (protozoa) counts per mLMiscellaneous Protozoan per mLVorticella(Protozoa) counts per mLNemertines per mLAbsorbance at 440nM in Spectrophotometer Model 20, which provides a measure of turbidity.First measurement of dissolved oxygen taken in the a.m. before the lights are turned on.Second measurement of dissolved oxygen taken in the p.m. before the lights are turned off.Third measurement of dissolved oxygen taken in the a.m. before the lights are turned on.The amount of gained dissolved oxygen during the light period. This is calculated by subtracting DO1 from DO2.The amount of lost dissolved oxygen during the dark period. This is calculated by subtracting DO3 from DO2.The change in dissolved oxygen over 24 hours. This is calculated by subtracting DO1 from DO3.The ratio of photosynthesis(gain of oxygen) and respiration(loss of oxygen). The equation is as follows: (DO2-DO1)/(DO2-DO3) = (DDOAM)/(DDOPM)In vivo Fluorescence measured in 1-cm diameter tube. Measured using Turner Model #111 fluorometer.pH taken immediately after lights are turned on at 930 hours.pH taken between 1130-1200 hours.
Storage Type:float  
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float  
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float  
Measurement Type:rationominalnominalratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratiorationominalratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratio
Measurement Values Domain:
UnitnominalDay
Typereal
Allowed Values and Definitions
Enumerated Domain 
Code Definition
Code1
DefinitionControl
Source
Code Definition
Code2
Definition500 ppb Cu^++
Source
Code Definition
Code3
Definition1000 ppb Cu^++
Source
Code Definition
Code4
Definition2000 ppb Cu^++
Source
DefinitionMicrocosm number (identifier)
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberMicrometerCubedPer0.1Mililiter
Typereal
UnitnumberMicrometerCubedPer0.1MilimeterCubed
Typereal
Unitdimensionless
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Unitdimensionless
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UnitnumberPer100Milliliters
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UnitnumberPer100Milliliters
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UnitnumberPer100Milliliters
Typereal
UnitnumberPer100Milliliters
Typereal
Unitdimensionless
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UnitnumberPer100Milliliters
Typereal
UnitnumberPer100Milliliters
Typereal
UnitnumberPer100Milliliters
Typereal
UnitnumberPer100Milliliters
Typereal
UnitnumberPer100Milliliters
Typereal
Allowed Values and Definitions
Enumerated Domain 
Code Definition
Code0
DefinitionEphippia is not present
Source
Code Definition
Code1
DefinitionEphippia is present
Source
UnitnumberPerMilliliter
Typereal
UnitnumberPerMilliliter
Typereal
UnitnumberPerMilliliter
Typereal
UnitnumberPerMilliliter
Typereal
UnitnumberPerMilliliter
Typereal
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Typereal
UnitopticalDensity
Typereal
UnitpartsPerMillion
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UnitpartsPerMillion
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UnitpartsPerMillion
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UnitpartsPerMillion
Typereal
UnitpartsPerMillion
Typereal
UnitpartsPerMillion
Typereal
Unitdimensionless
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Typereal
Unitdimensionless
Typereal
Missing Value Code:      
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Accuracy Report:                                                                                                
Accuracy Assessment:                                                                                                
Coverage:                                                                                                
Methods:                                                                                                

Data Table

Data:https://pasta-s.lternet.edu/package/data/eml/edi/1083/1/47964bd23ed5c7ed8c980b5743182e74
Name:ME89
Description:All data for experiment 89 from the University of Washington in Seattle with six replicates of 0, 500, 1000, and 2000 ppb Cu++.
Number of Records:456
Number of Columns:56

Table Structure
Object Name:ME89.csv
Size:113357 byte
Authentication:354a5837c44cc9f89e26ded40274cec7 Calculated By MD5
Text Format:
Number of Header Lines:1
Record Delimiter:\r\n
Orientation:column
Simple Delimited:
Field Delimiter:,
Quote Character:"

Table Column Descriptions
 DayTreatmentMicrocosm NumberSelenastrum DensityChlamydomonas DensityScenedesmus DensityAnkistrodesmus DensityChlorella DensityNitzschia Kutzigiana DensityAnabaena Cell DensityLyngbya Cell DesntiyStigeoclonium DensityUlothrix DensityUnidentified Green Alga DensityAvailable AlgaeAlgal BiovolumeDaphnia Fecundity RatioShannon-Weaver Weighted Algal DiversityShannon-Weaver Algal Species DiversitySmall Daphnia ConcentrationMedium Daphnia ConcentrationLarge Daphnia ConcentrationDaphnia ConcentrationSmall to Total Daphnia Concentration RatioDaphnia BiomassSmall Amphipods ConcentrationLarge Amphipods ConcentrationAmphipods ConcentrationOstracods ConcentrationEphippia PresenceRotifer ConcentrationSmall Rotifer ConcentrationHypotrichs ConcentrationMiscellaneous Protozoan ConcentrationVorticella CocentrationNemertines ConcentrationFirst Dissolved Oxygen MeasurementSecond Dissolved Oxygen MeasurementThird Dissolved Oxygen MeasurementDissolved Oxygen GainDissolved Oxygen LossDissolved Oxygen Change Over 24 HoursNet Photosynthesis-Respiration RatioIn vivo FluorescencepH Immediately After Lights OnpH at 1130-1200 HoursPhosphate ConcentrationSilicate ConcentrationNitrate ConcentrationNitrite ConcentrationAmmonia ConcentrationNitrate to Phosphate ratio(Nitrate + Nitrite) to Phosphate RatioTotal Nitrogen to Phosphate Ratio
Column Name:DAY  
TRT  
MIC  
SELAN  
CHLAMY  
SCENED  
ANKIS  
CHLOR  
D216  
ANABENA  
LYNGBYA  
STIG  
ULOTH  
UNKALG1  
AVALGAE  
ABIOVOL  
DFECUND  
DVRSFD  
DVRSALG  
SDAPHNIA  
MDAPHNIA  
LDAPHNIA  
DAPHNIA  
PSDAPHN  
MDSIZE  
DBIOMAS  
SAMPH  
LAMPH  
AMPH  
OSTRACOD  
EPHIPPIA  
PHILODIN  
ROTIFER  
HYPO  
MISCEL  
VORT  
NEMER  
TURBAB  
DO1  
DO2  
DO3  
DDOAM  
DDOPM  
DELDO  
RATIOPR  
FLUOR  
PH1  
PH2  
PO4  
SIO4  
NO3  
NO2  
NH3  
RATIO1  
RATIO2  
RATIO3  
Definition:Day since experiment started the sample was takenThe treatment number assigned to microcosm the data was collected fromMicrocosm number (identifier)Selenastrum abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Chlamydomonas abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Scenedesmus abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Ankistrodesmus abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Chlorella abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Nitzschia Kutzigiana abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Anabaena abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Lyngbya abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Stigeoclonium abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Ulothrix abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.Unidentified green alga abundance, counted in a 1mm x 1mm x 0.1mm hemocytometer.A measurement of the algae available for Daphnia to feed on based on the biomass of algae and their feeding availability.The measurement of the total algal biovolume of the algae in each microcosm determined by algae count and cell volume.Daphnia Fecundity Ratio. Calculated by SDAPHNIA/LDAPHNIA.An estimate of the diversity of algal cells that are available to the Daphnia for feedingDiversity measurements of all algal species from the Shannon-Weaver equation.Small Daphnia(~0.7mm) count per 100mLMedium Daphnia(0.7mm-1.8mm) count per 100mLLarge Daphnia(>1.8mm) count per 100mLDaphnia count per 100mLThe ratio of small to total daphniaMean Daphnia LengthDaphnia Biomass per 100MLSmall Amphipods(<4mm) per 100 mLLarge Amphipods(>4mm) per 100 mLAmphipods per 100 mLOstracods per 100mLEphippia presence in DaphniaPhilodina acuticornis (rotifer) per mLSmall Rotifers per mLHypotrichs (protozoa) counts per mLMiscellaneous Protozoan per mLVorticella(Protozoa) counts per mLNemertines per mLAbsorbance at 440nM in Spectrophotometer Model 20, which provides a measure of turbidity.First measurement of dissolved oxygen taken in the a.m. before the lights are turned on.Second measurement of dissolved oxygen taken in the p.m. before the lights are turned off.Third measurement of dissolved oxygen taken in the a.m. before the lights are turned on.The amount of gained dissolved oxygen during the light period. This is calculated by subtracting DO1 from DO2.The amount of lost dissolved oxygen during the dark period. This is calculated by subtracting DO3 from DO2.The change in dissolved oxygen over 24 hours. This is calculated by subtracting DO1 from DO3.The ratio of photosynthesis(gain of oxygen) and respiration(loss of oxygen). The equation is as follows: (DO2-DO1)/(DO2-DO3) = (DDOAM)/(DDOPM)In vivo Fluorescence measured in 1-cm diameter tube. Measured using Turner Model #111 fluorometer.pH taken immediately after lights are turned on at 930 hours.pH taken between 1130-1200 hours.Phosphate(PO4) concentrationSilicate(SiO4) concentrationNitrate NO3 ConcentrationNitrite(NO2) ConcentrationAmmonia(NH3) ConcentrationRatio of Nitrate to Phosphate. Calculated by NO3/PO4.Ratio of Nitrate and Nitrite to Phosphate. Calculated by (NO3 + NO2)/PO4.Total Nitrogen to Phosphate Ratio. Calculated by (NO3+NO2+NH3)/PO4.
Storage Type:float  
string  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
string  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
string  
float  
string  
float  
float  
float  
float  
float  
float  
float  
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float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
Measurement Type:rationominalnominalratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratiorationominalratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratio
Measurement Values Domain:
UnitnominalDay
Typereal
Allowed Values and Definitions
Enumerated Domain 
Code Definition
Code1
DefinitionControl
Source
Code Definition
Code2
Definition500 ppb Cu^++
Source
Code Definition
Code3
Definition1000 ppb Cu^++
Source
Code Definition
Code4
Definition2000 ppb Cu^++
Source
DefinitionMicrocosm number (identifier)
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberPer0.1MillimeterCubed
Typereal
UnitnumberMicrometerCubedPer0.1Mililiter
Typereal
UnitnumberMicrometerCubedPer0.1MilimeterCubed
Typereal
Unitdimensionless
Typereal
Unitdimensionless
Typereal
Unitdimensionless
Typereal
UnitnumberPer100Milliliters
Typereal
UnitnumberPer100Milliliters
Typereal
UnitnumberPer100Milliliters
Typereal
UnitnumberPer100Milliliters
Typereal
Unitdimensionless
Typereal
Unitmillimeter
Typereal
UnitnumberPer100Milliliters
Typereal
UnitnumberPer100Milliliters
Typereal
UnitnumberPer100Milliliters
Typereal
UnitnumberPer100Milliliters
Typereal
UnitnumberPer100Milliliters
Typereal
Allowed Values and Definitions
Enumerated Domain 
Code Definition
Code-99
DefinitionData is not available
Source
Code Definition
Code0
DefinitionEphippia is not present
Source
Code Definition
Code1
DefinitionEphippia is present
Source
UnitnumberPerMilliliter
Typereal
UnitnumberPerMilliliter
Typereal
UnitnumberPerMilliliter
Typereal
UnitnumberPerMilliliter
Typereal
UnitnumberPerMilliliter
Typereal
UnitnumberPerMilliliter
Typereal
UnitopticalDensity
Typereal
UnitpartsPerMillion
Typereal
UnitpartsPerMillion
Typereal
UnitpartsPerMillion
Typereal
UnitpartsPerMillion
Typereal
UnitpartsPerMillion
Typereal
UnitpartsPerMillion
Typereal
Unitdimensionless
Typereal
Unitdimensionless
Typereal
Unitdimensionless
Typereal
Unitdimensionless
Typereal
UnitmicrogramPerLiter
Typereal
UnitmicrogramPerLiter
Typereal
UnitmicrogramPerLiter
Typereal
UnitmicrogramPerLiter
Typereal
UnitmicrogramPerLiter
Typereal
Unitdimensionless
Typereal
Unitdimensionless
Typereal
Unitdimensionless
Typereal
Missing Value Code:      
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Code-99.0000
Expldata is not available
Accuracy Report:                                                                                                                
Accuracy Assessment:                                                                                                                
Coverage:                                                                                                                
Methods:                                                                                                                

Data Package Usage Rights

This information is released under the Creative Commons license - Attribution - CC BY (https://creativecommons.org/licenses/by/4.0/). The consumer of these data ("Data User" herein) is required to cite it appropriately in any publication that results from its use. The Data User should realize that these data may be actively used by others for ongoing research and that coordination may be necessary to prevent duplicate publication. The Data User is urged to contact the authors of these data if any questions about methodology or results occur. Where appropriate, the Data User is encouraged to consider collaboration or co-authorship with the authors. The Data User should realize that misinterpretation of data may occur if used out of context of the original study. While substantial efforts are made to ensure the accuracy of data and associated documentation, complete accuracy of data sets cannot be guaranteed. All data are made available "as is." The Data User should be aware, however, that data are updated periodically and it is the responsibility of the Data User to check for new versions of the data. The data authors and the repository where these data were obtained shall not be liable for damages resulting from any use or misinterpretation of the data. Thank you.

Keywords

By Thesaurus:
LTER Controlled Vocabularyalgae, biology, carbon cycling, copper, grazing, competition, rotifers, protozoa, freshwater, nitrate, nitrite, ammonia, phytoplankton
(No thesaurus)daphnia, microcosm, amphipod, ostracod, toxicity, Interlab testing, cyanobacteria, toxicity testing, Standardized Aquatic Microcosms, copper sulfate, silicate, phosphate, CuSO4 toxicity

Methods and Protocols

These methods, instrumentation and/or protocols apply to all data in this dataset:

Methods and protocols used in the collection of this data package
Description:

These methods, instrumentation, and protocols apply to all the data in this dataset.

Description:

Presented in ASTM. (2012). E1366 Standard Practice for Standardized Aquatic Microcosms: Fresh Water. Annual Book of Standards 11.06. https://www.astm.org/e1366-96.html

Taub, F. (1989). Standardized aquatic microcosm development and testing. In A. Boudou & F. Ribeyre (Eds.), Aquatic Ecotoxicology (Vol. II, pp. 47-94): CRC Press, Inc https://www.taylorfrancis.com/chapters/edit/10.1201/9781351069854-3/standardized-aquatic-microcosm-development-testing-taub

Description:

Description: Ten species of phytoplankton (day 0), five species of animals (day 4), and the test chemical, CuSO4, (day 7) were inoculated into a chemically defined freshwater microcosms (3 liters + a defined sediment). To allow the recovery of populations, small numbers of each species were reintroduced each week. Each experiment consisted of 4 treatment groups, control, low, medium, and high concentration. Ten experiments were performed within four different laboratories. All but one of the experiments used 0,500, 1000, and 2000 ppb Cu++ as CuSO4; one experiment, ME74, used 0, 127, 225, and 509 Cu++. Organism counts and some chemical measurements were taken twice a week for at least the first 4 weeks, and some were then reduced to once a week. Organism counts, oxygen concentrations (predawn, afternoon, predawn), in-vivo fluorescence (as a measure of Chl a), pH, and absorbance at 440 nM were performed twice weekly until day 63. Nutrient samples were taken twice a week for the first month and once weekly thereafter. If experiments did not pass quality control criteria, e.g., Daphnia populations were not statistically different than zero (ME 64, ME 69), experiments were redone (ME 82, ME 89). Much of the details of the medium, sediment, and variables measured are documented (ASTM, 2012; Taub, 1989). The medium satisfied all the nutritional requirements of the animals and phytoplankton, including trace metals and vitamins required by the animals. Exceptions, such as broken containers or other lab irregularities are given in the Experiment Specific Metadata data table.

Since the intent of the study was to perform an ecosystem assessment that would be independent of location, all materials were chemically defined, and all organisms were from laboratory cultures. To minimize the effects of outliers, initially, 30 microcosms (4-liter containers) were prepared and evaluated on day 7, and the 24 most similar microcosms were selected and randomly assigned to treatment groups.

People and Organizations

Publishers:
Organization:Environmental Data Initiative
Email Address:
info@environmentaldatainitiative.org
Web Address:
https://environmentaldatainitiative.org
Id:https://ror.org/0330j0z60
Creators:
Individual:Dr. Frieda B Taub
Organization:University of Washington
Position:Professor Emeritus
Address:
School of Aquatic and Fishery Sciences,
University of Washington,
Seattle, Washington 98105
Email Address:
taub@uw.edu
Id:https://orcid.org/0000-0002-0332-9122
Contacts:
Individual: Frieda B Taub
Organization:School of Aquatic and Fishery Sciences - University of Washington
Position:Professor Emeritus
Address:
School of Aquatic and Fishery Sciences,
University of Washington,
Seattle, Washington 98105
Email Address:
taub@uw.edu
Id:https://orcid.org/0000-0002-0332-9122
Individual: John Matheson
Organization:US FDA retired
Email Address:
Matheson.jc@gmail.com
Individual: James Meador
Organization:NOAA Fisheries
Position:Aquatic Toxicologist
Email Address:
jmeador2k@gmail.com
Web Address:
https://deohs.washington.edu/faculty/james-meador
Id:https://orcid.org/0000-0002-6861-8048
Metadata Providers:
Individual: Kate McGrath-Flinn
Organization:School of Aquatic and Fishery Sciences - University of Washington
Position:Research Assistant
Email Address:
katemf16@uw.edu

Temporal, Geographic and Taxonomic Coverage

Temporal, Geographic and/or Taxonomic information that applies to all data in this dataset:

Time Period
Begin:
1983-12-02
End:
1986-07-18
Geographic Region:
Description:University of Washington, Seattle, Washington, 98195. Experiments 64, 69, 72, 82, and 89 took place here.
Bounding Coordinates:
Northern:  47.6615413363Southern:  47.6474488015
Western:  -122.3174540002Eastern:  -122.2991720636
Geographic Region:
Description:EPA-Duluth Laboratory, University of Minnesota - Duluth. Experiments 65 and 67 took place here.
Bounding Coordinates:
Northern:  46.840022467Southern:  46.8148293598
Western:  -92.0918681655Eastern:  -92.0018639598
Geographic Region:
Description:U.S. Army Chemical Research & Development Center Aberdeen Proving Ground, Maryland 21010-5423. Experiments 74 and 87 took place here.
Bounding Coordinates:
Northern:  39.5056300573Southern:  39.4369115114
Western:  -76.1638839972Eastern:  -76.0959060919
Geographic Region:
Description:Marine Bioassay Laboratories, Inc. Carlsbad, California. Experiment 79 took here.
Bounding Coordinates:
Northern:  33.1818350625Southern:  33.088395753
Western:  -117.367259264Eastern:  -117.1965420246
Taxonomic Range:
Classification:
Rank Name:Kingdom
Rank Value:Plantae
Common Name:plants
Identifer:https://www.itis.gov
ID: 202422
Classification:
Rank Name:Subkingdom
Rank Value:Viridiplantae
Common Name:green plants
Identifer:https://www.itis.gov
ID: 954898
Classification:
Rank Name:Infrakingdom
Rank Value:Chlorophyta
Common Name:green algae
Identifer:https://www.itis.gov
ID: 846493
Classification:
Rank Name:Division
Rank Value:Chlorophyta
Common Name:green algae
Identifer:https://www.itis.gov
ID: 5414
Classification:
Rank Name:Subdivision
Rank Value:Chlorophytina
Identifer:https://www.itis.gov
ID: 846499
Classification:
Rank Name:Class
Rank Value:Chlorophyceae
Identifer:https://www.itis.gov
ID: 5415
Classification:
Rank Name:Order
Rank Value:Sphaeropleales
Identifer:https://www.itis.gov
ID: 9327
Classification:
Rank Name:Family
Rank Value:Selenastraceae
Identifer:https://www.itis.gov
ID: 954992
Classification:
Rank Name:Genus
Rank Value:Selenastrum
Identifer:https://www.itis.gov
ID: 6249
Classification:
Rank Name:Species
Rank Value:Selenastrum capricornutum
Identifer:https://www.itis.gov
ID: 6257
Taxonomic Range:
Classification:
Rank Name:Kingdom
Rank Value:Bacteria
Common Name:bacteria
Identifer:https://www.itis.gov
ID: 50
Classification:
Rank Name:Subkingdom
Rank Value:Negibacteria
Identifer:https://www.itis.gov
ID: 956096
Classification:
Rank Name:Phylum
Rank Value:Cyanobacteria
Common Name:blue-green algae
Identifer:https://www.itis.gov
ID: 956108
Classification:
Rank Name:Class
Rank Value:Cyanophyceae
Identifer:https://www.itis.gov
ID: 602
Classification:
Rank Name:Order
Rank Value:Nostocales
Identifer:https://www.itis.gov
ID: 861
Classification:
Rank Name:Family
Rank Value:Nostocaceae
Identifer:https://www.itis.gov
ID: 1099
Classification:
Rank Name:Genus
Rank Value:Anabaena
Identifer:https://www.itis.gov
ID: 1100
Classification:
Rank Name:Species
Rank Value:Anabaena cylindrica
Identifer:https://www.itis.gov
ID: 1128
Taxonomic Range:
Classification:
Rank Name:Kingdom
Rank Value:Viridiplantae
Common Name:green plants
Identifer:National Center for Biotechnology Information - Taxonomy (NCBI)
Info for ID: 33090 (Viridiplantae)
Classification:
Rank Name:Phylum
Rank Value:Chlorophyta
Common Name:green algae
Identifer:National Center for Biotechnology Information - Taxonomy (NCBI)
Info for ID: 3041 (Chlorophyta)
Classification:
Rank Name:Class
Rank Value:Trebouxiophyceae
Identifer:National Center for Biotechnology Information - Taxonomy (NCBI)
Info for ID: 75966 (Trebouxiophyceae)
Classification:
Rank Name:Order
Rank Value:Chlorellales
Identifer:National Center for Biotechnology Information - Taxonomy (NCBI)
Info for ID: 35460 (Chlorellales)
Classification:
Rank Name:Family
Rank Value:Chlorellaceae
Identifer:National Center for Biotechnology Information - Taxonomy (NCBI)
Info for ID: 35461 (Chlorellaceae)
Classification:
Rank Name:Genus
Rank Value:Chlorella
Identifer:National Center for Biotechnology Information - Taxonomy (NCBI)
Info for ID: 3071 (Chlorella)
Classification:
Rank Name:Species
Rank Value:Chlorella vulgaris
Identifer:National Center for Biotechnology Information - Taxonomy (NCBI)
Info for ID: 3077 (Chlorella vulgaris)
Taxonomic Range:
Classification:
Rank Name:Kingdom
Rank Value:Plantae
Common Name:plants
Identifer:https://www.itis.gov
ID: 202422
Classification:
Rank Name:Subkingdom
Rank Value:Viridiplantae
Common Name:green plants
Identifer:https://www.itis.gov
ID: 954898
Classification:
Rank Name:Infrakingdom
Rank Value:Chlorophyta
Common Name:green algae
Identifer:https://www.itis.gov
ID: 846493
Classification:
Rank Name:Division
Rank Value:Chlorophyta
Common Name:green algae
Identifer:https://www.itis.gov
ID: 5414
Classification:
Rank Name:Subdivision
Rank Value:Chlorophytina
Identifer:https://www.itis.gov
ID: 846499
Classification:
Rank Name:Class
Rank Value:Chlorophyceae
Identifer:https://www.itis.gov
ID: 5415
Classification:
Rank Name:Order
Rank Value:Sphaeropleales
Identifer:https://www.itis.gov
ID: 9327
Classification:
Rank Name:Family
Rank Value:Selenastraceae
Identifer:https://www.itis.gov
ID: 954992
Classification:
Rank Name:Genus
Rank Value:Ankistrodesmus
Identifer:https://www.itis.gov
ID: 5877
Taxonomic Range:
Classification:
Rank Name:Kingdom
Rank Value:Viridiplantae
Common Name:green plants
Identifer:National Center for Biotechnology Information - Taxonomy (NCBI)
Info for ID: 33090 (Viridiplantae)
Classification:
Rank Name:Phylum
Rank Value:Chlorophyta
Common Name:green algae
Identifer:National Center for Biotechnology Information - Taxonomy (NCBI)
Info for ID: 3041 (Chlorophyta)
Classification:
Rank Name:Class
Rank Value:Chlorophyceae
Identifer:National Center for Biotechnology Information - Taxonomy (NCBI)
Info for ID: 3166 (Chlorophyceae)
Classification:
Rank Name:Order
Rank Value:Chlamydomonadales
Identifer:National Center for Biotechnology Information - Taxonomy (NCBI)
Info for ID: 3042 (Chlamydomonadales)
Classification:
Rank Name:Family
Rank Value:Chlamydomonadaceae
Identifer:National Center for Biotechnology Information - Taxonomy (NCBI)
Info for ID: 3051 (Chlamydomonadaceae)
Classification:
Rank Name:Genus
Rank Value:Chlamydomonas
Identifer:National Center for Biotechnology Information - Taxonomy (NCBI)
Info for ID: 3052 (Chlamydomonas)
Classification:
Rank Name:Species
Rank Value:Chlamydomonas reinhardtii
Identifer:National Center for Biotechnology Information - Taxonomy (NCBI)
Info for ID: 3055 (Chlamydomonas reinhardtii)
Taxonomic Range:
Classification:
Rank Name:Kingdom
Rank Value:Bacteria
Common Name:bacteria
Identifer:https://www.itis.gov
ID: 50
Classification:
Rank Name:Subkingdom
Rank Value:Negibacteria
Identifer:https://www.itis.gov
ID: 956096
Classification:
Rank Name:Phylum
Rank Value:Cyanobacteria
Common Name:blue-green algae
Identifer:https://www.itis.gov
ID: 956108
Classification:
Rank Name:Class
Rank Value:Cyanophyceae
Identifer:https://www.itis.gov
ID: 602
Classification:
Rank Name:Order
Rank Value:Nostocales
Identifer:https://www.itis.gov
ID: 861
Classification:
Rank Name:Family
Rank Value:Oscillatoriaceae
Identifer:https://www.itis.gov
ID: 862
Classification:
Rank Name:Genus
Rank Value:Lyngbya
Identifer:https://www.itis.gov
ID: 870
Taxonomic Range:
Classification:
Rank Name:Species
Rank Value:Scenedesmus obliquus
Identifer:https://www.itis.gov
ID: 6160
Taxonomic Range:
Classification:
Rank Name:Species
Rank Value:Raphidocelis subcapitata
Identifer:https://www.itis.gov
ID: 6160
Taxonomic Range:
Classification:
Rank Name:Kingdom
Rank Value:Plantae
Common Name:plants
Identifer:https://www.itis.gov
ID: 202422
Classification:
Rank Name:Subkingdom
Rank Value:Viridiplantae
Common Name:green plants
Identifer:https://www.itis.gov
ID: 954898
Classification:
Rank Name:Infrakingdom
Rank Value:Chlorophyta
Common Name:green algae
Identifer:https://www.itis.gov
ID: 846493
Classification:
Rank Name:Division
Rank Value:Chlorophyta
Common Name:green algae
Identifer:https://www.itis.gov
ID: 5414
Classification:
Rank Name:Subdivision
Rank Value:Chlorophytina
Identifer:https://www.itis.gov
ID: 846499
Classification:
Rank Name:Class
Rank Value:Chlorophyceae
Identifer:https://www.itis.gov
ID: 5415
Classification:
Rank Name:Order
Rank Value:Chaetophorales
Identifer:https://www.itis.gov
ID: 6594
Classification:
Rank Name:Family
Rank Value:Chaetophoraceae
Identifer:https://www.itis.gov
ID: 6595
Classification:
Rank Name:Genus
Rank Value:Stigeoclonium
Identifer:https://www.itis.gov
ID: 6651
Taxonomic Range:
Classification:
Rank Name:Kingdom
Rank Value:Plantae
Common Name:plants
Identifer:https://www.itis.gov
ID: 202422
Classification:
Rank Name:Subkingdom
Rank Value:Viridiplantae
Common Name:green plants
Identifer:https://www.itis.gov
ID: 954898
Classification:
Rank Name:Infrakingdom
Rank Value:Chlorophyta
Common Name:green algae
Identifer:https://www.itis.gov
ID: 846493
Classification:
Rank Name:Division
Rank Value:Chlorophyta
Common Name:green algae
Identifer:https://www.itis.gov
ID: 5414
Classification:
Rank Name:Subdivision
Rank Value:Chlorophytina
Identifer:https://www.itis.gov
ID: 846499
Classification:
Rank Name:Class
Rank Value:Ulvophyceae
Identifer:https://www.itis.gov
ID: 846521
Classification:
Rank Name:Order
Rank Value:Ulotrichales
Identifer:https://www.itis.gov
ID: 6409
Classification:
Rank Name:Family
Rank Value:Ulotrichaceae
Identifer:https://www.itis.gov
ID: 6410
Classification:
Rank Name:Genus
Rank Value:Ulothrix
Identifer:https://www.itis.gov
ID: 6417
Taxonomic Range:
Classification:
Rank Name:Kingdom
Rank Value:Animalia
Common Name:animals
Identifer:https://www.itis.gov
ID: 202423
Classification:
Rank Name:Subkingdom
Rank Value:Bilateria
Identifer:https://www.itis.gov
ID: 914154
Classification:
Rank Name:Infrakingdom
Rank Value:Protostomia
Identifer:https://www.itis.gov
ID: 914155
Classification:
Rank Name:Superphylum
Rank Value:Ecdysozoa
Identifer:https://www.itis.gov
ID: 914158
Classification:
Rank Name:Phylum
Rank Value:Arthropoda
Common Name:arthropods
Identifer:https://www.itis.gov
ID: 82696
Classification:
Rank Name:Subphylum
Rank Value:Crustacea
Common Name:crustaceans
Identifer:https://www.itis.gov
ID: 83677
Classification:
Rank Name:Class
Rank Value:Branchiopoda
Common Name:branchiopods
Identifer:https://www.itis.gov
ID: 83687
Classification:
Rank Name:Order
Rank Value:Diplostraca
Identifer:https://www.itis.gov
ID: 83767
Classification:
Rank Name:Suborder
Rank Value:Cladocera
Common Name:water fleas
Identifer:https://www.itis.gov
ID: 83832
Classification:
Rank Name:Infraorder
Rank Value:Anomopoda
Identifer:https://www.itis.gov
ID: 609958
Classification:
Rank Name:Family
Rank Value:Daphniidae
Identifer:https://www.itis.gov
ID: 83872
Classification:
Rank Name:Genus
Rank Value:Daphnia
Identifer:https://www.itis.gov
ID: 83873
Classification:
Rank Name:Species
Rank Value:Daphnia magna
Identifer:https://www.itis.gov
ID: 83884
Taxonomic Range:
Classification:
Rank Name:Kingdom
Rank Value:Animalia
Common Name:animals
Identifer:https://www.itis.gov
ID: 202423
Classification:
Rank Name:Subkingdom
Rank Value:Bilateria
Identifer:https://www.itis.gov
ID: 914154
Classification:
Rank Name:Infrakingdom
Rank Value:Protostomia
Identifer:https://www.itis.gov
ID: 914155
Classification:
Rank Name:Superphylum
Rank Value:Ecdysozoa
Identifer:https://www.itis.gov
ID: 914158
Classification:
Rank Name:Phylum
Rank Value:Arthropoda
Common Name:arthropods
Identifer:https://www.itis.gov
ID: 82696
Classification:
Rank Name:Subphylum
Rank Value:Crustacea
Common Name:crustaceans
Identifer:https://www.itis.gov
ID: 83677
Classification:
Rank Name:Class
Rank Value:Malacostraca
Identifer:https://www.itis.gov
ID: 89787
Classification:
Rank Name:Subclass
Rank Value:Eumalacostraca
Identifer:https://www.itis.gov
ID: 89801
Classification:
Rank Name:Superorder
Rank Value:Peracarida
Identifer:https://www.itis.gov
ID: 573755
Classification:
Rank Name:Order
Rank Value:Amphipoda
Common Name:amphipods
Identifer:https://www.itis.gov
ID: 93294
Classification:
Rank Name:Suborder
Rank Value:Gammaridea
Common Name:gammarid amphipods
Identifer:https://www.itis.gov
ID: 93295
Classification:
Rank Name:Family
Rank Value:Hyalellidae
Identifer:https://www.itis.gov
ID: 94022
Classification:
Rank Name:Genus
Rank Value:Hyalella
Identifer:https://www.itis.gov
ID: 94025
Classification:
Rank Name:Species
Rank Value:Hyalella azteca
Identifer:https://www.itis.gov
ID: 94026
Taxonomic Range:
Classification:
Rank Name:Kingdom
Rank Value:Animalia
Common Name:animals
Identifer:https://www.itis.gov
ID: 202423
Classification:
Rank Name:Subkingdom
Rank Value:Bilateria
Identifer:https://www.itis.gov
ID: 914154
Classification:
Rank Name:Infrakingdom
Rank Value:Protostomia
Identifer:https://www.itis.gov
ID: 914155
Classification:
Rank Name:Superphylum
Rank Value:Ecdysozoa
Identifer:https://www.itis.gov
ID: 914158
Classification:
Rank Name:Phylum
Rank Value:Arthropoda
Common Name:arthropods
Identifer:https://www.itis.gov
ID: 82696
Classification:
Rank Name:Subphylum
Rank Value:Crustacea
Common Name:crustaceans
Identifer:https://www.itis.gov
ID: 83677
Classification:
Rank Name:Class
Rank Value:Ostracoda
Common Name:ostracods
Identifer:https://www.itis.gov
ID: 84195
Classification:
Rank Name:Subclass
Rank Value:Podocopa
Identifer:https://www.itis.gov
ID: 84409
Classification:
Rank Name:Order
Rank Value:Podocopida
Identifer:https://www.itis.gov
ID: 609939
Classification:
Rank Name:Suborder
Rank Value:Cypridocopina
Identifer:https://www.itis.gov
ID: 609945
Classification:
Rank Name:Superfamily
Rank Value:Cypridoidea
Identifer:https://www.itis.gov
ID: 609968
Classification:
Rank Name:Family
Rank Value:Cyprididae
Identifer:https://www.itis.gov
ID: 84462
Classification:
Rank Name:Genus
Rank Value:Cypridopsis
Identifer:https://www.itis.gov
ID: 85213
Taxonomic Range:
Classification:
Rank Name:Kingdom
Rank Value:Protozoa
Taxonomic Range:
Classification:
Rank Name:Phylum
Rank Value:Ciliophora
Common Name:ciliates
Identifer:National Center for Biotechnology Information - Taxonomy (NCBI)
Info for ID: 5878 (Ciliophora)
Classification:
Rank Name:Subphylum
Rank Value:Intramacronucleata
Identifer:National Center for Biotechnology Information - Taxonomy (NCBI)
Info for ID: 431838 (Intramacronucleata)
Classification:
Rank Name:Class
Rank Value:Spirotrichea
Identifer:National Center for Biotechnology Information - Taxonomy (NCBI)
Info for ID: 33829 (Spirotrichea)
Classification:
Rank Name:Subclass
Rank Value:Hypotrichia
Identifer:National Center for Biotechnology Information - Taxonomy (NCBI)
Info for ID: 5934 (Hypotrichia)
Taxonomic Range:
Classification:
Rank Name:Kingdom
Rank Value:Animalia
Common Name:animals
Identifer:https://www.itis.gov
ID: 202423
Classification:
Rank Name:Subkingdom
Rank Value:Bilateria
Identifer:https://www.itis.gov
ID: 914154
Classification:
Rank Name:Infrakingdom
Rank Value:Protostomia
Identifer:https://www.itis.gov
ID: 914155
Classification:
Rank Name:Superphylum
Rank Value:Platyzoa
Identifer:https://www.itis.gov
ID: 914160
Classification:
Rank Name:Phylum
Rank Value:Rotifera
Common Name:rotifers
Identifer:https://www.itis.gov
ID: 58239
Classification:
Rank Name:Class
Rank Value:Bdelloidea
Identifer:https://www.itis.gov
ID: 914205
Classification:
Rank Name:Subclass
Rank Value:Bdelloidea
Identifer:https://www.itis.gov
ID: 58246
Classification:
Rank Name:Family
Rank Value:Philodinidae
Identifer:https://www.itis.gov
ID: 58266
Classification:
Rank Name:Genus
Rank Value:Philodina
Identifer:https://www.itis.gov
ID: 58276
Classification:
Rank Name:Species
Rank Value:Philodina acuticornis
Identifer:https://www.itis.gov
ID: 58277

Project

Parent Project Information:

Title:Interlaboratory Testing of Aquatic Microcosm Protocol
Personnel:
Individual:Dr. Frieda B Taub
Organization:University of Washington
Position:Professor Emeritus
Address:
School of Aquatic and Fishery Sciences,
University of Washington,
Seattle, Washington 98105
Email Address:
taub@uw.edu
Id:https://orcid.org/0000-0002-0332-9122
Role:Principal Investigator
Individual: John Matheson
Email Address:
matheson.jc@gmail.com
Role:FDA Project Officer
Additional Award Information:
Funder:United States Food and Drug Administration
Number:223-83-7000
Title:Interlaboratory Testing of Aquatic Microcosm Protocol

Maintenance

Maintenance:
Description:

This data set is complete and has no updates planned in the future.

Frequency:notPlanned
Other Metadata

Additional Metadata

additionalMetadata
        |___text '\n        '
        |___element 'metadata'
        |     |___text '\n            '
        |     |___element 'unitList'
        |     |     |___text '\n                '
        |     |     |___element 'unit'
        |     |     |     |  \___attribute 'id' = 'numberPer100Milliliters'
        |     |     |     |  \___attribute 'name' = 'numberPer100Milliliters'
        |     |     |     |___text '\n                    '
        |     |     |     |___element 'description'
        |     |     |     |     |___text 'Large organisms counted in 100ml subsamples.'
        |     |     |     |___text '\n                '
        |     |     |___text '\n                '
        |     |     |___element 'unit'
        |     |     |     |  \___attribute 'id' = 'partsPerMillion'
        |     |     |     |  \___attribute 'name' = 'partsPerMillion'
        |     |     |     |___text '\n                    '
        |     |     |     |___element 'description'
        |     |     |     |___text '\n                '
        |     |     |___text '\n                '
        |     |     |___element 'unit'
        |     |     |     |  \___attribute 'id' = 'numberPer0.1MillimeterCubed'
        |     |     |     |  \___attribute 'name' = 'numberPer0.1MillimeterCubed'
        |     |     |     |___text '\n                    '
        |     |     |     |___element 'description'
        |     |     |     |     |___text 'Phytoplankton abundance, counted in a 1mm x 1mm x 0.1mm\n                        hemocytometer. For the number of cells per ml, multiply values by\n                        10^4.'
        |     |     |     |___text '\n                '
        |     |     |___text '\n                '
        |     |     |___element 'unit'
        |     |     |     |  \___attribute 'id' = 'numberMicrometerCubedPer0.1Mililiter'
        |     |     |     |  \___attribute 'name' = 'numberMicrometerCubedPer0.1Mililiter'
        |     |     |     |___text '\n                    '
        |     |     |     |___element 'description'
        |     |     |     |     |___text 'Phytoplankton abundance, counted in a 1mm x 1mm x 0.1mm\n                        hemocytometer, multiplied by a factor based on feeding availability and\n                        biomass as micrometers cubed.'
        |     |     |     |___text '\n                '
        |     |     |___text '\n                '
        |     |     |___element 'unit'
        |     |     |     |  \___attribute 'id' = 'numberMicrometerCubedPer0.1MilimeterCubed'
        |     |     |     |  \___attribute 'name' = 'numberMicrometerCubedPer0.1MilimeterCubed'
        |     |     |     |___text '\n                    '
        |     |     |     |___element 'description'
        |     |     |     |     |___text 'Phytoplankton abundance, counted in a 1mm x 1mm x 0.1mm\n                        hemocytometer, multiplied by biomass as micrometers cubed.'
        |     |     |     |___text '\n                '
        |     |     |___text '\n                '
        |     |     |___element 'unit'
        |     |     |     |  \___attribute 'id' = 'opticalDensity'
        |     |     |     |  \___attribute 'name' = 'opticalDensity'
        |     |     |     |___text '\n                    '
        |     |     |     |___element 'description'
        |     |     |     |     |___text 'The negative log 10 of percent transmission as measured at 440nM in\n                        a Spectrophotometer, Model 20.'
        |     |     |     |___text '\n                '
        |     |     |___text '\n            '
        |     |___text '\n        '
        |___text '\n    '

Additional Metadata

additionalMetadata
        |___text '\n        '
        |___element 'metadata'
        |     |___text '\n            '
        |     |___element 'emlEditor'
        |     |        \___attribute 'app' = 'ezEML'
        |     |        \___attribute 'release' = '2022.02.04'
        |     |___text '\n        '
        |___text '\n    '

EDI is a collaboration between the University of New Mexico and the University of Wisconsin – Madison, Center for Limnology:

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