Data Package Metadata View Summary

Soil extracellular enzyme activities in plots dominated by trees that associate with arbuscular mycorrhizal or ectomycorrhizal fungi in the N fertilized and reference watershed at the Fernow Experimental Forest, WV.

General Information

Data Package:

Local Identifier:

edi.1234.1

Title:

Soil extracellular enzyme activities in plots dominated by trees that associate with arbuscular mycorrhizal or ectomycorrhizal fungi in the N fertilized and reference watershed at the Fernow Experimental Forest, WV.

Alternate Identifier:

DOI PLACE HOLDER

Abstract:

Our objective was to detect possible differences in N fertilization responses of soil extracellular enzymes in plots dominated by trees that associate with arbuscular mycorrhizal fungi (AM) or ectomycorrhizal fungi (ECM). To do this, we established a plot network of 6 AM and 6 ECM dominated 10 x 10 m plots in both the reference and N fertilized watersheds (N=24 plots) at the Fernow Experimental Forest, Parsons, WV. We assayed the potential activity of hydrolytic enzymes that release N (N-acetylglucosaminidase; NAG), phosphorus (acid phosphatase; AP), and simple carbon (ß-glucosidase; BG). In addition, we measured microbial allocation to complex C degrading oxidative enzymes phenol oxidase and peroxidase. The activities of these enzymes were measured separately in bulk mineral, rhizosphere, and organic horizon soils during the growing season in 2017.

Publication Date:

2022-10-05

For more information:
Visit:	DOI PLACE HOLDER

Time Period

Begin:

2017-05-01

End:

2017-09-30

People and Organizations
Contact:	Carrara, Joseph E (West Virginia University) [ email ]
Creator:	Carrara, Joseph E (West Virginia University)
Creator:	Brzostek, Edward R (West Virginia University)
Creator:	Raczka, Nanette C (West Virginia University)

Data Entities
Data Table Name:	Fernow_data
Description:	Soil extracellular enzyme activities in AM and ECM dominated plots in the N fertilized and reference Watershed at the Fernow Experimental Forest, WV, USA.

Detailed Metadata

Data Entities

Data Table


Data:	https://pasta-s.lternet.edu/package/data/eml/edi/1234/1/9f3a3adf091ec2b87d9d9d29b362d033
Name:	Fernow_data
Description:	Soil extracellular enzyme activities in AM and ECM dominated plots in the N fertilized and reference Watershed at the Fernow Experimental Forest, WV, USA.
Number of Records:	72
Number of Columns:	9

Table Structure

Object Name:

Fernow_data.csv

Size:

7238 byte

Authentication:

8665c250e297d620573878cbdd58626b Calculated By MD5

Text Format:

Number of Header Lines:

Record Delimiter:

\r\n

Orientation:

column

Simple Delimited:

Field Delimiter:	,
Quote Character:	"

Table Column Descriptions

Plot

Mycorrhizal_type

Watershed

Fraction

NAG

Phenox

Perox

Column Name:

Plot

Mycorrhizal_type

Watershed

Fraction

NAG

Phenox

Perox

Definition:

plot number ID

dominant tree mycorrhizal type in plot

Treatment of the watershed

Soil type wherein the enzymes were measured

Activity of the enzyme acid phosphatase (phosphorus acquiring enzyme), expressed in MicromolsPerGramDrySoilPerHour

Activity of the enzyme beta-glucosidase (simple carbon acquiring enzyme), expressed in MicromolsPerGramDrySoilPerHour

Activity of the enzyme n-acetylglucosaminidase (nitrogen acquiring enzyme), expressed in MicromolsPerGramDrySoilPerHour

Activity of the enzyme phenol oxidase (complex carbon acquiring enzyme), expressed in MicromolsPerGramDrySoilPerHour

Activity of the enzyme peroxidase (complex carbon acquiring enzyme), expressed in MicromolsPerGramDrySoilPerHour

Storage Type:

string

float

Measurement Type:

nominal

ratio

Measurement Values Domain:

Definition

plot number ID

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	arbuscular
Definition	arbuscular mycorrhizae
Source

Code Definition

Code	ectomycorrhizal
Definition	ectomycorrhizal fungi
Source

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	Control
Definition	Reference
Source

Code Definition

Code	Fertilized
Definition	Nitrogen Fertilized
Source

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	Bulk
Definition	Bulk mineral soil
Source

Code Definition

Code	OrganicHorizon
Definition	Organic Horizon Soil
Source

Code Definition

Code	Rhizosphere
Definition	Rhizosphere soil
Source

Unit	micromolePerGramPerHour
Type	real

Unit	micromolePerGramPerHour
Type	real

Unit	micromolePerGramPerHour
Type	real

Unit	micromolePerGramPerHour
Type	real

Unit	micromolePerGramPerHour
Type	real

Missing Value Code:

Accuracy Report:

Accuracy Assessment:

Coverage:

Methods:

Data Package Usage Rights

This data package is released to the "public domain" under Creative Commons CC0 1.0 "No Rights Reserved" (see: https://creativecommons.org/publicdomain/zero/1.0/). It is considered professional etiquette to provide attribution of the original work if this data package is shared in whole or by individual components. A generic citation is provided for this data package on the website https://portal.edirepository.org (herein "website") in the summary metadata page. Communication (and collaboration) with the creators of this data package is recommended to prevent duplicate research or publication. This data package (and its components) is made available "as is" and with no warranty of accuracy or fitness for use. The creators of this data package and the website shall not be liable for any damages resulting from misinterpretation or misuse of the data package or its components. Periodic updates of this data package may be available from the website. Thank you.

Keywords

By Thesaurus:
(No thesaurus)	extracellular enzymes , arbuscular mycorrhizae, ectomycorrhizae, temperate forests, Fernow Experimental Forest, nitrogen fertilization
LTER Controlled Vocabulary	soil carbon, biogeochemistry, nutrient cycling

Methods and Protocols

These methods, instrumentation and/or protocols apply to all data in this dataset:

Methods and protocols used in the collection of this data package

Description:

To examine potential differences in the impacts of long-term N fertilization on root-microbial interactions between mycorrhizal types, we established a plot network of 6 AM and 6 ECM dominated (>65% diameter at breast height) 10x10 m plots in the fertilized and reference watersheds. Dominant AM and ECM trees were similar between watersheds with AM trees represented by Tulip poplar (Liriodendron tulipifera), red maple (Acer rubrum), black cherry (Prunus serotina), and sugar maple (Acer saccharum). Dominant ECM trees were sweet birch (Betula lenta), red oak (Quercus rubra), and American beech (Fagus grandifolia).

To capture variability across the growing season, we sampled soils in each plot in May, June, and September of 2017. In each plot we extracted three 10 x 10 cm organic horizon layers and homogenized them into a single sample defining this as the OH soil fraction. Next, we sampled four 5 cm diameter mineral soil cores to a depth of 15 cm beneath the OH layer and homogenized these by plot. All samples were kept on ice and transported to West Virginia University for further processing within 48-72 h. Upon return to the lab, we separated rhizosphere soil from mineral soil samples via the soil-adhesion method wherein the rhizosphere soil fraction was operationally defined as soil that remained clung to roots after modest shaking (Phillips & Fahey, 2005). After removal of roots, all soils were passed through a 2 mm sieve and stored at -80°C until further analysis.

To determine the extent to which N fertilization impacts microbial allocation to extracellular enzymes, we assayed the potential activity of hydrolytic enzymes that release N (N-acetylglucosaminidase; NAG), phosphorus (acid phosphatase; AP), and simple carbon (ß-glucosidase; BG). In addition, we measured microbial allocation to complex C degrading oxidative enzymes phenol oxidase and peroxidase. Briefly, 1g of thawed soil was homogenized in 50mM sodium acetate buffer (pH 5.0). Next, hydrolytic activities were determined using a fluorometric microplate assay (Gemini XPS, Molecular Devices, USA). with methylumbelliferone-linked substrates and oxidative enzymes using a colorimetric microplate assay (Tecan Infinite 200 Pro, Switzerland) with L-3,4-dihydroxyphenylalanine linked substrates according to Saiya-Cork et al., 2002.

Saiya-Cork KR, Sinsabaugh RL, Zak DR (2002) The effects of long term nitrogen deposition on extracellular enzyme activity in an Acer saccharum forest soil. Soil Biology and Biochemistry, 34, 1309–1315. DOI: 10.1016/S0038-0717(02)00074-3.

See protocol: https://allison.bio.uci.edu/protocols/fluorimetricenzymeprotocol.pdf

People and Organizations

Publishers:

Organization:

Environmental Data Initiative

Email Address:

info@edirepository.org

Web Address:

https://edirepository.org

Id:

https://ror.org/0330j0z60

Creators:

Individual:

Joseph E Carrara

Organization:

West Virginia University

Email Address:

jocarrara@mix.wvu.edu

Individual:

Edward R Brzostek

Organization:

West Virginia University

Email Address:

erbrzostek@mail.wvu.edu

Individual:

Nanette C Raczka

Organization:

West Virginia University

Email Address:

nanetteraczka@gmail.com

Contacts:

Individual:

Joseph E Carrara

Organization:

West Virginia University

Email Address:

jocarrara@mix.wvu.edu

Temporal, Geographic and Taxonomic Coverage

Temporal, Geographic and/or Taxonomic information that applies to all data in this dataset:

Time Period

Begin:

2017-05-01

End:

2017-09-30

Sampling Site:

Description:

The watershed-level nitrogen fertilization experiment at Fernow Experimental Forest (herein Fernow) has been used to study the impact of forest acidification on soil carbon and nutrient cycling dynamics for 30 years. The site is located in the central Appalachian Mountains near the community of Parsons, West Virginia (39.03°N, 79.67°W). The experiment consists of two adjacent watersheds (fertilized and reference) of similar stand age (~48 years old) and tree species composition. The N fertilized watershed (34.2ha; Watershed 3) was fertilized aerially at a rate of 35 kg N ha-1 yr-1 in the form of solid pellet (NH4)2 SO4 from 1989-2019.

Site Coordinates:

Longitude (degree):

79.6701

Latitude (degree):

39.0543

Project

Parent Project Information:

Title:

Fernow Experimental Forest Nitrogen Fertilization Soil Enzymes

Personnel:

Individual:

Joseph E Carrara

Organization:

West Virginia University

Email Address:

jocarrara@mix.wvu.edu

Role:

graduate student

Individual:

Edward R Brzostek

Organization:

West Virginia University

Email Address:

erbrzostek@mail.wvu.edu

Role:

Principle investigator

Maintenance

Maintenance:

Description:	Data collection has ceased, and data will not be added to this data set.
Frequency:	notPlanned

Other Metadata

Additional Metadata

additionalMetadata
        |___text '\n    '
        |___element 'metadata'
        |     |___text '\n      '
        |     |___element 'emlEditor'
        |     |        \___attribute 'app' = 'ezEML'
        |     |        \___attribute 'release' = '2022.09.28'
        |     |___text '\n    '
        |___text '\n  '

Copyright 2024 Environmental Data Initiative. This material is based upon work supported by the National Science Foundation under grants #2223103 and #2223104. Any opinions, findings, conclusions, or recommendations expressed in the material are those of the author(s) and do not necessarily reflect the views of the National Science Foundation. Please contact us with questions, comments, or for technical assistance regarding this web site or the Environmental Data Initiative. Please read our privacy policy to know what information we collect about you and to understand your privacy rights.

EDI is a collaboration between the University of New Mexico and the University of Wisconsin – Madison, Center for Limnology:

Data Package Metadata View Summary

Soil extracellular enzyme activities in plots dominated by trees that associate with arbuscular mycorrhizal or ectomycorrhizal fungi in the N fertilized and reference watershed at the Fernow Experimental Forest, WV.

Data Entities

Data Table

Data Package Usage Rights

Keywords

Methods and Protocols

These methods, instrumentation and/or protocols apply to all data in this dataset:

People and Organizations

Temporal, Geographic and Taxonomic Coverage

Project

Parent Project Information:

Maintenance

Additional Metadata

Recently Added

Recently Updated

Data Package Metadata View Summary

Soil extracellular enzyme activities in plots dominated by trees that associate with arbuscular mycorrhizal or ectomycorrhizal fungi in the N fertilized and reference watershed at the Fernow Experimental Forest, WV.

+/- Data Entities

Data Table

+/- Data Package Usage Rights

+/- Keywords

+/- Methods and Protocols

These methods, instrumentation and/or protocols apply to all data in this dataset:

+/- People and Organizations

+/- Temporal, Geographic and Taxonomic Coverage

+/- Project

Parent Project Information:

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Data Package Usage Rights

Keywords

Methods and Protocols

People and Organizations

Temporal, Geographic and Taxonomic Coverage

Project

Maintenance

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