Data Package Metadata View Summary

Exposure to sublethal concentrations of a pesticide or predator cues induces changes in brain architecture in larval amphibians, 2013.

General Information

Data Package:

Local Identifier:

edi.1649.2

Title:

Exposure to sublethal concentrations of a pesticide or predator cues induces changes in brain architecture in larval amphibians, 2013.

Alternate Identifier:

DOI PLACE HOLDER

Abstract:

Naturally occurring environmental factors shape developmental trajectories to produce variable phenotypes. Such developmental phenotypic plasticity can have important effects on fitness, and has been demonstrated for numerous behavioral and morphological traits. However, surprisingly few studies have examined developmental plasticity of the nervous system in response to naturally occurring environmental variation, despite accumulating evidence for neuroplasticity in a variety of organisms. Here, we asked whether the brain is developmentally plastic by exposing larval amphibians to natural and anthropogenic factors. Leopard frog tadpoles were exposed to predator cues, reduced food availability, or sublethal concentrations of the pesticide chlorpyrifos in semi-natural enclosures. Mass, growth, survival, activity, larval period, external morphology, brain mass, and brain morphology were measured in tadpoles and after metamorphosis. Tadpoles in the experimental treatments had lower masses than controls, although developmental rates and survival were similar. Tadpoles exposed to predator cues or a high dose of chlorpyrifos had altered body shapes compared to controls. In addition, brains from tadpoles exposed to predator cues or a low dose of chlorpyrifos were narrower and shorter in several dimensions compared to control tadpoles and tadpoles with low food availability. Interestingly, the changes in brain morphology present at the tadpole stage did not persist in the metamorphs. Our results show that brain morphology is a developmentally plastic trait that is responsive to ecologically relevant natural and anthropogenic factors. Whether these effects on brain morphology are linked to performance or fitness is unknown.

Publication Date:

2024-05-30

For more information:
Visit:	DOI PLACE HOLDER

Time Period

Begin:

2013

End:

2013

People and Organizations
Contact:	Relyea, Rick (Rensselaer Polytechnic Institute) [ email ]
Creator:	Relyea, Rick A. (Rensselaer Polytechnic Institute)
Creator:	Woodley, Sarah (Duquesne University)
Creator:	Mattes, Brian (Rensselaer Polytechnic Institute)
Creator:	Yates, Erika A. (Rensselaer Polytechnic Institute)

Data Entities
Data Table Name:	tadpole_data_combined
Description:	Tadpole survival, mass, brain morphology, and body morphology.
Data Table Name:	metamorph_data_combined
Description:	Data on survival to metamorphosis, metamorph mass, and mass-adjusted metamorph shape in leopard frogs exposed to control conditions, predator cues, low food, or two concentrations of CPF (organophosphate pesticide chlorpyrifos).

Detailed Metadata

Data Entities

Data Table


Data:	https://pasta-s.lternet.edu/package/data/eml/edi/1649/2/16dd5524445a89b24fd1329edd2fbaba
Name:	tadpole_data_combined
Description:	Tadpole survival, mass, brain morphology, and body morphology.
Number of Records:	20
Number of Columns:	28

Table Structure

Object Name:

tadpole_data_combined.csv

Size:

3157 byte

Authentication:

206df1ed16d06562fd334e7a4b523e6c Calculated By MD5

Text Format:

Number of Header Lines:

Record Delimiter:

\r\n

Orientation:

column

Simple Delimited:

Field Delimiter:	,
Quote Character:	"

Table Column Descriptions

Treatment

Mesocosm

MA_brainmass

brainmass

survivorship

MA_TW

MA__TL

MA_OTW

MA_OTL

MA_ML

MA_MD

MA_DW

MA_DL

MA_OBL

MA_MW

BodyMass

GosnerStage

Activity

Seen

bodymass

gosnerstage

MA_BodyWidth

MA_BodyLength

MA_BodyDepth

MA_TailDepth

MA_MuscleDepth

MA_TailLength

MA_MuscWidth

Column Name:

Treatment

Mesocosm

MA_brainmass

brainmass

survivorship

MA_TW

MA__TL

MA_OTW

MA_OTL

MA_ML

MA_MD

MA_DW

MA_DL

MA_OBL

MA_MW

BodyMass

GosnerStage

Activity

Seen

bodymass

gosnerstage

MA_BodyWidth

MA_BodyLength

MA_BodyDepth

MA_TailDepth

MA_MuscleDepth

MA_TailLength

MA_MuscWidth

Definition:

Experimental treatment

Cattle tank mesocosm #

Mass-adjusted brain mass

Brain mass

Survival of tadpoles

Brain mass-adjusted telencephalon width

Brain mass-adjusted telencephalon length

Brain mass-adjusted optic tectum width

Brain mass-adjusted optic tectum length

Brain mass-adjusted medulla length

Brain mass-adjusted medulla width

Brain mass-adjusted diencephalon width

Brain mass-adjusted diencephalon length

Brain mass-adjusted olfactory bulb length

Brain mass-adjusted medulla width

Tadpole mass

Gosner developmental stage

Proportion of tadpoles moving

Number of tadpoles seen in mesocosm

Body mass (redundant)

Gosner developmental stage (redundant)

Mass-adjusted body width

Mass-adjusted body length

Mass-adjusted body depth

Mass-adjusted tail depth

Mass-adjusted muscle depth

Mass-adjusted tail length

Mass-adjusted tail muscle width

Storage Type:

string

float

Measurement Type:

nominal

ratio

Measurement Values Domain:

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	comp
Definition	Competition
Source

Code Definition

Code	control
Definition	Control treatment
Source

Code Definition

Code	high chlor
Definition	high chlorpyrifos
Source

Code Definition

Code	low chlor
Definition	Low chlorpyrifos
Source

Code Definition

Code	pred
Definition	Predator cues
Source

Unit	Number
Type	integer

Unit	milligram
Type	real

Unit	milligram
Type	real

Unit	percent
Type	real

Unit	millimeter
Type	real

Unit	millimeter
Type	real

Unit	millimeter
Type	real

Unit	millimeter
Type	real

Unit	millimeter
Type	real

Unit	millimeter
Type	real

Unit	millimeter
Type	real

Unit	millimeter
Type	real

Unit	millimeter
Type	real

Unit	millimeter
Type	real

Unit	gram
Type	real

Unit	Gosner stage
Type	real

Unit	percent
Type	real

Unit	Number
Type	real

Unit	gram
Type	real

Unit	Gosner stage
Type	real

Unit	millimeter
Type	real

Unit	millimeter
Type	real

Unit	millimeter
Type	real

Unit	millimeter
Type	real

Unit	millimeter
Type	real

Unit	millimeter
Type	real

Unit	millimeter
Type	real

Missing Value Code:

Accuracy Report:

Accuracy Assessment:

Coverage:

Methods:

Data Table


Data:	https://pasta-s.lternet.edu/package/data/eml/edi/1649/2/c6383c4f35f01f217c32577814275615
Name:	metamorph_data_combined
Description:	Data on survival to metamorphosis, metamorph mass, and mass-adjusted metamorph shape in leopard frogs exposed to control conditions, predator cues, low food, or two concentrations of CPF (organophosphate pesticide chlorpyrifos).
Number of Records:	20
Number of Columns:	31

Table Structure

Object Name:

metamorph_data_combined.csv

Size:

4669 byte

Authentication:

968a6d1be88cbd9546f7954f9a91bc72 Calculated By MD5

Text Format:

Number of Header Lines:

Record Delimiter:

\r\n

Orientation:

column

Simple Delimited:

Field Delimiter:	,
Quote Character:	"

Table Column Descriptions

Treatment

Mesocosm

MA_TW

MA_TL

MA_OTW

MA_OTL

MA_ML

MA_BL

MA_MD

MA_DW

MA_DL

MA_OBL

MA_MW

larvalperiod

survivorship

bodymass

MA_BodyLength

MA_HeadWidth

MA_BodyWidth

MA_LeftForelimbLength

MA_LeftForelimbWidth

MA_LeftThighLength

MA_LeftThighWidth

MA_LegLength

MA_LeftFootLength

MA_RightForelimbLength

MA_RightForelimbWidth

MA_RightThighLength

MA_RightThighWidth

MA_RightLegLength

MA_RightFootLength

Column Name:

Treatment

Mesocosm

MA_TW

MA_TL

MA_OTW

MA_OTL

MA_ML

MA_BL

MA_MD

MA_DW

MA_DL

MA_OBL

MA_MW

larvalperiod

survivorship

bodymass

MA_BodyLength

MA_HeadWidth

MA_BodyWidth

MA_LeftForelimbLength

MA_LeftForelimbWidth

MA_LeftThighLength

MA_LeftThighWidth

MA_LegLength

MA_LeftFootLength

MA_RightForelimbLength

MA_RightForelimbWidth

MA_RightThighLength

MA_RightThighWidth

MA_RightLegLength

MA_RightFootLength

Definition:

Exprimental treatments

Cattle tank mesocosm #

Brain mass-adjusted telencephalon width

Brain mass-adjusted telencephalon length

Brain mass-adjusted optic tectum width

Brain mass-adjusted optic tectum length

Brain mass-adjusted medulla length

Mass-adjusted brain length

Brain mass-adjusted medulla depth

Brain mass-adjusted diencephalon width

Brain mass-adjusted diencephalon length

Mass-adjusted olfactory bulb length

Brain mass-adjusted medulla width

Larval period

Survival to metamorphosis

Mass at metamorphosis at end of the experiment

Mass-adjusted body length

Mass-adjusted head width

Mass-adjusted body width

Mass-adjusted left forelimb length

Mass-adjusted left forelimb width

Mass-adjusted left thigh length

Mass-adjusted left thigh width

Mass-adjusted left leg length

Mass-adjusted left foot length

Mass-adjusted right forelimb length

Mass-adjusted right forelimb width

Mass-adjusted right thigh length

Mass-adjusted right thigh width

Mass-adjusted right leg length

Mass-adjusted right foot length

Storage Type:

string

float

Measurement Type:

nominal

ratio

Measurement Values Domain:

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	competition
Definition	Competition
Source

Code Definition

Code	control
Definition	Control treatment
Source

Code Definition

Code	high chlor
Definition	high chlorpyrifos
Source

Code Definition

Code	low chlor
Definition	Low chlorpyrifos
Source

Code Definition

Code	predator
Definition	Predator cues
Source

Unit	Number
Type	integer

Unit	millimeter
Type	real

Unit	millimeter
Type	real

Unit	millimeter
Type	real

Unit	millimeter
Type	real

Unit	millimeter
Type	real

Unit	millimeter
Type	real

Unit	millimeter
Type	real

Unit	millimeter
Type	real

Unit	millimeter
Type	real

Unit	millimeter
Type	real

Unit	millimeter
Type	real

Unit	days
Type	integer

Unit	percent
Type	integer

Unit	gram
Type	real

Unit	millimeter
Type	real

Unit	millimeter
Type	real

Unit	millimeter
Type	real

Unit	millimeter
Type	real

Unit	millimeter
Type	real

Unit	millimeter
Type	real

Unit	millimeter
Type	real

Unit	millimeter
Type	real

Unit	millimeter
Type	real

Unit	millimeter
Type	real

Unit	millimeter
Type	real

Unit	millimeter
Type	real

Unit	millimeter
Type	real

Unit	millimeter
Type	real

Unit	millimeter
Type	real

Missing Value Code:

Accuracy Report:

Accuracy Assessment:

Coverage:

Methods:

Data Package Usage Rights

This information is released under the Creative Commons license - Attribution - CC BY (https://creativecommons.org/licenses/by/4.0/). The consumer of these data ("Data User" herein) is required to cite it appropriately in any publication that results from its use. The Data User should realize that these data may be actively used by others for ongoing research and that coordination may be necessary to prevent duplicate publication. The Data User is urged to contact the authors of these data if any questions about methodology or results occur. Where appropriate, the Data User is encouraged to consider collaboration or co-authorship with the authors. The Data User should realize that misinterpretation of data may occur if used out of context of the original study. While substantial efforts are made to ensure the accuracy of data and associated documentation, complete accuracy of data sets cannot be guaranteed. All data are made available "as is." The Data User should be aware, however, that data are updated periodically and it is the responsibility of the Data User to check for new versions of the data. The data authors and the repository where these data were obtained shall not be liable for damages resulting from any use or misinterpretation of the data. Thank you.

Keywords

By Thesaurus:
(No thesaurus)	Predator, Brain plasticity, Brain shape, Metamorphosis, Lithobates pipiens, Insecticide

Methods and Protocols

These methods, instrumentation and/or protocols apply to all data in this dataset:

Methods and protocols used in the collection of this data package

Description:

All methods are detailed in: Woodley, S.K., Mattes, B.M., Yates, E.K. et al. Exposure to sublethal concentrations of a pesticide or predator cues induces changes in brain architecture in larval amphibians. Oecologia 179, 655–665 (2015). https://doi.org/10.1007/s00442-015-3386-3

People and Organizations

Publishers:

Organization:

Environmental Data Initiative

Email Address:

info@edirepository.org

Web Address:

https://edirepository.org

Id:

https://ror.org/0330j0z60

Creators:

Individual:

Rick A. Relyea

Organization:

Rensselaer Polytechnic Institute

Address:

Troy, NY 12180 US

Phone:

5182768342 (voice)

Email Address:

relyer@rpi.edu

Id:

https://orcid.org/0000-0001-6693-6950

Individual:

Sarah Woodley

Organization:

Duquesne University

Email Address:

woodleys@duq.edu

Individual:

Brian Mattes

Organization:

Rensselaer Polytechnic Institute

Address:

Troy, NY 12180

Email Address:

matteb2@rpi.edu

Individual:

Erika A. Yates

Organization:

Rensselaer Polytechnic Institute

Address:

Troy, NY 12180 US

Contacts:

Individual:

Rick Relyea

Organization:

Rensselaer Polytechnic Institute

Address:

Troy, NY 12180 US

Phone:

5182768342 (voice)

Email Address:

relyer@rpi.edu

Id:

https://orcid.org/0000-0001-6693-6950

Temporal, Geographic and Taxonomic Coverage

Temporal, Geographic and/or Taxonomic information that applies to all data in this dataset:

Time Period

Begin:

2013

End:

2013

Sampling Site:

Description:

Pymatuning lab of Ecology

Site Coordinates:

Longitude (degree):

-80.455998

Latitude (degree):

41.569769

Project

Parent Project Information:

Title:

Woodley, S. K., B. M. Mattes, E. K. Yates, and R. A. Relyea. 2015. Exposure to sublethal concentrations of a pesticide or predator cues induces changes in brain architecture in larval amphibians. Oecologia 179:655-665.

Personnel:

Individual:

Rick A. Relyea

Organization:

Rensselaer Polytechnic Institute

Address:

Troy, NY 12180 US

Phone:

5182768342 (voice)

Email Address:

relyer@rpi.edu

Role:

Individual:

Brian Mattes

Organization:

Rensselaer Polytechnic Institute

Address:

Troy, NY 12180

Role:

Technician

Individual:	Erika Yates
Role:	Technician

Individual:	Sarah Woodley
Organization:	Duquesne Univ.
Role:	Co-PI

Additional Award Information:

Funder:	NSF
Number:	11-19430
Title:	Chemical stressor effects on amphibians: From individuals to ecosystems

Additional Award Information:

Funder:	Duquesne Univ.
Number:	Internal funding to Sarah Woodley
Title:	N/A

Other Metadata

Additional Metadata

additionalMetadata
        |___text '\n      '
        |___element 'metadata'
        |     |___text '\n         '
        |     |___element 'unitList'
        |     |     |___text '\n            '
        |     |     |___element 'unit'
        |     |     |     |  \___attribute 'id' = 'Number'
        |     |     |     |  \___attribute 'name' = 'Number'
        |     |     |     |___text '\n               '
        |     |     |     |___element 'description'
        |     |     |     |___text '\n            '
        |     |     |___text '\n            '
        |     |     |___element 'unit'
        |     |     |     |  \___attribute 'id' = 'Gosner stage'
        |     |     |     |  \___attribute 'name' = 'Gosner stage'
        |     |     |     |___text '\n               '
        |     |     |     |___element 'description'
        |     |     |     |___text '\n            '
        |     |     |___text '\n            '
        |     |     |___element 'unit'
        |     |     |     |  \___attribute 'id' = 'days'
        |     |     |     |  \___attribute 'name' = 'days'
        |     |     |     |___text '\n               '
        |     |     |     |___element 'description'
        |     |     |     |___text '\n            '
        |     |     |___text '\n            '
        |     |     |___element 'unit'
        |     |     |     |  \___attribute 'id' = 'millimeter'
        |     |     |     |  \___attribute 'name' = 'millimeter'
        |     |     |     |___text '\n               '
        |     |     |     |___element 'description'
        |     |     |     |___text '\n            '
        |     |     |___text '\n         '
        |     |___text '\n      '
        |___text '\n   '

Additional Metadata

additionalMetadata
        |___text '\n      '
        |___element 'metadata'
        |     |___text '\n         '
        |     |___element 'emlEditor'
        |     |        \___attribute 'app' = 'ezEML'
        |     |        \___attribute 'release' = '2024.05.22'
        |     |___text '\n      '
        |___text '\n   '

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EDI is a collaboration between the University of New Mexico and the University of Wisconsin – Madison, Center for Limnology:

Data Package Metadata View Summary

Exposure to sublethal concentrations of a pesticide or predator cues induces changes in brain architecture in larval amphibians, 2013.

Data Entities

Data Table

Data Table

Data Package Usage Rights

Keywords

Methods and Protocols

These methods, instrumentation and/or protocols apply to all data in this dataset:

People and Organizations

Temporal, Geographic and Taxonomic Coverage

Project

Parent Project Information:

Additional Metadata

Additional Metadata

Recently Added

Recently Updated

Data Package Metadata View Summary

Exposure to sublethal concentrations of a pesticide or predator cues induces changes in brain architecture in larval amphibians, 2013.

+/- Data Entities

Data Table

Data Table

+/- Data Package Usage Rights

+/- Keywords

+/- Methods and Protocols

These methods, instrumentation and/or protocols apply to all data in this dataset:

+/- People and Organizations

+/- Temporal, Geographic and Taxonomic Coverage

+/- Project

Parent Project Information:

+/- Additional Metadata

+/- Additional Metadata

Data Entities

Data Package Usage Rights

Keywords

Methods and Protocols

People and Organizations

Temporal, Geographic and Taxonomic Coverage

Project

Additional Metadata

Additional Metadata