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Uranium accumulation in Brassica juncea plant facilitated by calcium in carbonate water

General Information
Data Package:
Local Identifier:edi.17.3
Title:Uranium accumulation in Brassica juncea plant facilitated by calcium in carbonate water
Abstract:

The role of calcium (Ca) on the cellular distribution of U(VI) in Brassica juncea roots and root-to-shoot translocation was investigated using hydroponic experiments, microscopy, and spectroscopy. Uranium accumulated mainly in the roots (727−9376 mg kg−1) after 30 days of exposure to 80 μM dissolved U in water containing 1 mM HCO3 − at different Ca concentrations (0−6 mM) at pH 7.5. However, the concentration of U in the shoots increased 22 times in experiments with 6 mM Ca compared to 0 mM Ca. In the Ca control experiment, transmission electron microscopy−energy-dispersive spectroscopy analyses detected U−P-bearing precipitates in the cortical apoplast of parenchyma cells. In experiments with 0.3 mM Ca, U−P-bearing precipitates were detected in the cortical apoplast and the bordered pits of xylem cells. In experiments with 6 mM Ca, U−P-bearing precipitates aggregated in the xylem with no apoplastic precipitation. These results indicate that Ca in carbonate water inhibits the transport and precipitation of U in the root cortical apoplast and facilitates the symplastic transport and translocation toward shoots. These findings reveal the considerable role of Ca in the presence of carbonate in facilitating the transport of U in plants and present new insights for future assessment and phytoremediation strategies.

Publication Date:2020-05-22

Time Period
Begin:
2019
End:
2020

People and Organizations
Contact:El Hayek, Eliane (Department of Chemistry and Chemical Biology, University of New Mexico) [  email ]
Creator:El Hayek, Eliane (Department of Chemistry and Chemical Biology, University of New Mexico)
Associate:Brearley, Adrian J. (Department of Earth and Planetary Sciences, University of New Mexico, Investigator)
Associate:Howard, Tamara (Department of Cell Biology and Physiology, University of New Mexico, Investigator)
Associate:Hudson, Patrick (Department of Biology, University of New Mexico, Investigator)
Associate:Torres, Chris (Department of Chemical and Biological Engineering, University of New Mexico, Investigator)
Associate:Spilde, Michael N. (Department of Earth and Planetary Sciences, University of New Mexico, Investigator)
Associate:Cabaniss, Stephen (Department of Chemistry and Chemical Biology, University of New Mexico, Investigator)
Associate:Ali, Abdul-Medhi S. (Department of Earth and Planetary Sciences, University of New Mexico, Investigator)
Associate:Cerrato, Jose M. (Department of Civil Engineering, University of New Mexico, Investigator)

Data Entities
Data Table Name:
U in roots and shoots
Description:
U accumulation in roots and shoots controlled experiment data
Detailed Metadata

Data Entities

Data Table

Data:https://pasta-s.lternet.edu/package/data/eml/edi/17/3/58f72b909a05943337c8a1f19420137b
Name:U in roots and shoots
Description:U accumulation in roots and shoots controlled experiment data
Number of Records:34
Number of Columns:5

Table Structure
Object Name:rootsShootsU.csv
Size:1490 bytes
Authentication:44222d61567156465a0fc06ac0c486ee Calculated By MD5
Text Format:
Number of Header Lines:1
Record Delimiter:\r\n
Orientation:column
Simple Delimited:
Field Delimiter:,

Table Column Descriptions
 
Column Name:plantSample  
plantSampleMass  
UDigestedSolution  
massUDigestedSolution  
massAccumU  
Definition:Describes if plant sample is taken from root or shootMass of plant sample in gramsUranium concentration in digested solutionMass of uranium in 0.015 ml digested solutionMass of accumulated uranium in dry weight plant samples
Storage Type:string  
float  
float  
float  
float  
Measurement Type:nominalratioratioratioratio
Measurement Values Domain:
DefinitionDescribes if plant sample is taken from root or shoot
Unitgram
Typereal
Min0.048 
Max0.139 
UnitmicrogramPerLiter
Typereal
Min5.498 
Max1716.747666 
Unitmicrogram
Typereal
Min0.08247 
Max25.75121499 
UnitmilligramPerKilogram
Typereal
Min1.853724067 
Max23265.625 
Missing Value Code:
Code-99999
ExplMissing value
Code-99999
ExplMissing value
Code-99999
ExplMissing value
Code-99999
ExplMissing value
Code-99999
ExplMissing value
Accuracy Report:          
Accuracy Assessment:          
Coverage:          
Methods:          

Data Package Usage Rights

This information is released under the Creative Commons license - Attribution - CC BY (https://creativecommons.org/licenses/by/4.0/). The consumer of these data ("Data User" herein) is required to cite it appropriately in any publication that results from its use. The Data User should realize that these data may be actively used by others for ongoing research and that coordination may be necessary to prevent duplicate publication. The Data User is urged to contact the authors of these data if any questions about methodology or results occur. Where appropriate, the Data User is encouraged to consider collaboration or co-authorship with the authors. The Data User should realize that misinterpretation of data may occur if used out of context of the original study. While substantial efforts are made to ensure the accuracy of data and associated documentation, complete accuracy of data sets cannot be guaranteed. All data are made available "as is." The Data User should be aware, however, that data are updated periodically and it is the responsibility of the Data User to check for new versions of the data. The data authors and the repository where these data were obtained shall not be liable for damages resulting from any use or misinterpretation of the data. Thank you.

Keywords

By Thesaurus:
(No thesaurus)uranium, carbonate, plant roots, cellular distribution, transport pathways
LTER Controlled Vocabularycalcium, translocation

Methods and Protocols

These methods, instrumentation and/or protocols apply to all data in this dataset:

Methods and protocols used in the collection of this data package
Description:

Full methodology described in detail in associated manuscript linked in this EDI data package. Healthy 2 month old B. juncea seedlings were selected for exposure experiments and acclimatized to the chemical composition of the exposure solutions for 5 days in 400 mL solution containing simplified Hoagland nutrients but without U at pH 5.8−6.2. The chemical composition of the exposure solutions (0.5 mM MgSO4, 2 mM NH4NO3, 1 mM KCl, 1 mM NaHCO3, and 3 mM CaCl2·2H2O) was depleted of phosphate to avoid any improper U phosphate complexation/precipitation.

B juncea seedlings were exposed for 30 days to 80 uM U added as UO2 (NO3)2 (1000 mg L−1, Sigma-Aldrich). The exposure solutions consisted of fresh ultrapure water containing simplified Hoagland nutrients but presenting three different Ca treatments: (1) first treatment corresponds to 0 mM Ca, where ultrapure water was free of any added Ca; (2) the second treatment was prepared by adding 0.3 mM Ca to the solution; (3) and the third treatment was prepared by adding 6 mM Ca to the solution. All of the exposure solutions contained 2 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer to keep the pH stable at 7.5. Three replicate polypropylene (PP) bottles (n = 3) were used for each Ca treatment and for controls (solutions with no added U). Each bottle contained one seedling and 400 mL of exposure solution. All of the PP bottles were cleaned in dilute nitric acid bath (10%) and washed in ultrapure water before using them in the experiments. The total U content in acid-digested root and shoots was analyzed using inductively coupled plasma optical emission spectrometry (ICP-OES) and inductively coupled plasma mass spectrometry (ICP-MS).

People and Organizations

Creators:
Individual: Eliane El Hayek
Organization:Department of Chemistry and Chemical Biology, University of New Mexico
Email Address:
elhayek@unm.edu
Contacts:
Individual: Eliane El Hayek
Organization:Department of Chemistry and Chemical Biology, University of New Mexico
Email Address:
elhayek@unm.edu
Associated Parties:
Individual: Adrian J. Brearley
Organization:Department of Earth and Planetary Sciences, University of New Mexico
Role:Investigator
Individual: Tamara Howard
Organization:Department of Cell Biology and Physiology, University of New Mexico
Role:Investigator
Individual: Patrick Hudson
Organization:Department of Biology, University of New Mexico
Role:Investigator
Individual: Chris Torres
Organization:Department of Chemical and Biological Engineering, University of New Mexico
Role:Investigator
Individual: Michael N. Spilde
Organization:Department of Earth and Planetary Sciences, University of New Mexico
Role:Investigator
Individual: Stephen Cabaniss
Organization:Department of Chemistry and Chemical Biology, University of New Mexico
Role:Investigator
Individual: Abdul-Medhi S. Ali
Organization:Department of Earth and Planetary Sciences, University of New Mexico
Role:Investigator
Individual: Jose M. Cerrato
Organization:Department of Civil Engineering, University of New Mexico
Role:Investigator

Temporal, Geographic and Taxonomic Coverage

Temporal, Geographic and/or Taxonomic information that applies to all data in this dataset:

Time Period
Begin:
2019
End:
2020
Taxonomic Range:
Classification:
Rank Name:unknown
Rank Value:Brassica juncea

Project

Parent Project Information:

Title:Understanding Reactivity in American Native Impacted Uranium Mines (URANIUM): Research, Education and Outreach
Personnel:
Individual: Jose M. Cerrato
Organization:Department of Civil Engineering, University of New Mexico
Role:Principal Investigator
Funding: NSF 1652619
Related Project:
Title:Energize New Mexico EPSCoR Research Infrastructure
Personnel:
Individual: William Michener
Organization:University of New Mexico
Role:Principal Investigator
Funding: NSF 1301346
Related Project:
Title:CREST Center for Water and the Environment
Personnel:
Individual: Kerry Howe
Organization:University of New Mexico
Role:Principal Investigator
Funding: NSF 1345169
Related Project:
Title:UNM METALS Superfund Research Center
Personnel:
Individual: Johnnye L. Lewis
Organization:University of New Mexico Community Environmental Health Program
Role:Principal Investigator
Funding: NIEHS 1 P42 ES025589

Maintenance

Maintenance:
Description:completed
Frequency:

Additional Info

Additional Information:
 

Associated manuscript: https://doi.org/10.1021/acsearthspacechem.9b00171

Other Metadata

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