Data Package Metadata   View Summary

Data and code from: A mixture of grass-legume cover crop species may ameliorate water stress in a changing climate, a greenhouse experiment at Dickinson College in Carlisle, PA, USA, 2021.

General Information
Data Package:
Local Identifier:edi.1703.1
Title:Data and code from: A mixture of grass-legume cover crop species may ameliorate water stress in a changing climate, a greenhouse experiment at Dickinson College in Carlisle, PA, USA, 2021.
Alternate Identifier:DOI PLACE HOLDER
Abstract:

Data and R code associated with a greenhouse study investigating the influence of water stress on growth, root traits, and biomass of rye and crimson clover seedlings grown separately or together. Data were collected in the Dr. Inge P. Stafford Greenhouse of Dickinson College (Carlisle PA, USA) in June 2021.

Publication Date:2024-06-29
For more information:
Visit: DOI PLACE HOLDER

Time Period
Begin:
2021-06-01
End:
2021-07-16

People and Organizations
Contact:Truong, Nhu (Unravel Carbon Pte Ltd) [  email ]
Contact:Douglas, Margaret R. (Dickinson College) [  email ]
Creator:Truong, Nhu Quynh (Unravel Carbon Pte Ltd)
Creator:York, Larry M. (Oak Ridge National Laboratory)
Creator:Decker, Allyssa (Dickinson College)
Creator:Douglas, Margaret R. (Dickinson College)

Data Entities
Data Table Name:
Dry weight
Description:
The table contains total dry biomass and biomass per plant for each species, water treatment, species number, and replicate.
Data Table Name:
Survival rate
Description:
The table provides the survival rate by species, water treatment, species number, and replicate. Survival proportion was calculated from comparing number of survived seedlings at the termination of the experiment to the pot's density.
Data Table Name:
Crimson Clover's growth score
Description:
The table provides daily growth score of crimson clover by replicate, water stress, species number, and phase (i.e., water treatment and recovery). Growth score was determined by the emergence of cotyledons, first true leaf, first trifoliate leaf, second trifoliate leaf, etc.; each stage was equivalent to one point.
Data Table Name:
Rye's growth score
Description:
The table provides daily growth score of rye by replicate, water stress, species number, and phase (i.e., water treatment and recovery). One point of growth score indicated a visible leaf collar.
Data Table Name:
Crimson Clover's normalized root scan outputs
Description:
This table contains the outputs of Crimson Clover root scans from RhizoVision Explorer after normalization by the number of plants per pot. Variables are organized by water stress, species number, replicate, number of plants per pot.
Data Table Name:
Rye's normalized root scan outputs
Description:
This table contains the outputs of Rye root scans from RhizoVision Explorer after normalization by the number of plants per pot. Variables are organized by water stress, species number, replicate, number of plants per pot.
Data Table Name:
Crimson Clover's raw root scan outputs
Description:
This table contains the outputs of Crimson Clover root scans from RhizoVision Explorer. Variables are organized by water stress, species number, replicate, number of plants per pot.
Data Table Name:
Rye's raw root scan outputs
Description:
This table contains the outputs of Rye root scans from RhizoVision Explorer. Variables are organized by water stress, species number, replicate, number of plants per pot.
Other Name:
dryweight_cc_anova
Description:
Code to analyze the effect of water stress treatment, species number, and their interaction on per-plant dry biomass of crimson clover seedlings.
Other Name:
dryweight_r_glm
Description:
Code to analyze the effect of water stress treatment, species number, and their interaction on dry biomass of rye seedlings.
Other Name:
dryweight_totalbiomass
Description:
Code to analyze the effect of water stress treatment, species number, and their interaction on total biomass of crimson clover and rye grown separately or together.
Other Name:
growth_rate
Description:
Code to analyze the effect of water stress treatment, species number, and their interaction on growth rate of crimson clover and rye.
Other Name:
root_PCA_v2
Description:
Code for a principal component analysis to understand the influence of water stress and species number on the root morphology of crimson clover and rye.
Other Name:
root_analysis
Description:
Code to analyze the effect of water stress treatment, species number, and their interaction on key root traits in crimson clover and rye. Traits included total root length (both species), average root diameter (crimson clover), and maximum root diameter (rye).
Other Name:
survival_rate
Description:
Code to analyze the effect of water stress treatment, species number, and their interaction on seedling survival of crimson clover and rye.
Detailed Metadata

Data Entities


Data Table

Data:https://pasta-s.lternet.edu/package/data/eml/edi/1703/1/8b2091045a5e5918b45d37541d12e3cb
Name:Dry weight
Description:The table contains total dry biomass and biomass per plant for each species, water treatment, species number, and replicate.
Number of Records:48
Number of Columns:7

Table Structure
Object Name:dw.csv
Size:2387 byte
Authentication:11a955f3471687f563dcce02be5b90d0 Calculated By MD5
Text Format:
Number of Header Lines:1
Record Delimiter:\r\n
Orientation:column
Simple Delimited:
Field Delimiter:,
Quote Character:"

Table Column Descriptions
 repspeciesdiv_treatwater_treatliving_plantsshoot_massmass_per_plant
Column Name:rep  
species  
div_treat  
water_treat  
living_plants  
shoot_mass  
mass_per_plant  
Definition:Identity of each replicateSpecies of cover cropDiversity treatment in the experimentWater stress treatment in the experimentNumber of living plants in each potDry biomass of plant shoots at the end of the experimentDry biomass per plant
Storage Type:string  
string  
string  
string  
float  
float  
float  
Measurement Type:nominalnominalnominalnominalratioratioratio
Measurement Values Domain:
Allowed Values and Definitions
Enumerated Domain 
Code Definition
Code1
DefinitionFirst replicate
Source
Code Definition
Code2
DefinitionSecond replicate
Source
Code Definition
Code3
DefinitionThird replicate
Source
Code Definition
Code4
DefinitionFourth replicate
Source
Allowed Values and Definitions
Enumerated Domain 
Code Definition
CodeCrimson Clover
DefinitionCrimson clover (Trifolium incarnatum)
Source
Code Definition
CodeRye
DefinitionRye (Secale cereale)
Source
Allowed Values and Definitions
Enumerated Domain 
Code Definition
CodeMonoculture
DefinitionSingle species by itself
Source
Code Definition
CodePolyculture
DefinitionBiculture of rye and crimson clover
Source
Allowed Values and Definitions
Enumerated Domain 
Code Definition
CodeControl
DefinitionMaintained at 100% field capacity
Source
Code Definition
CodeDrought
DefinitionWithheld water for one week
Source
Code Definition
CodeWaterlogging
DefinitionLeft flooded at 5 mm above the surface for one week
Source
Unitnumber
Typeinteger
Unitgram
Typereal
Unitgram
Typereal
Missing Value Code:              
Accuracy Report:              
Accuracy Assessment:              
Coverage:              
Methods:              

Data Table

Data:https://pasta-s.lternet.edu/package/data/eml/edi/1703/1/76018fbd2592ad6839982392a2f68710
Name:Survival rate
Description:The table provides the survival rate by species, water treatment, species number, and replicate. Survival proportion was calculated from comparing number of survived seedlings at the termination of the experiment to the pot's density.
Number of Records:48
Number of Columns:6

Table Structure
Object Name:survive.csv
Size:1949 byte
Authentication:43187a9d41888a371700f4c2fcb691c3 Calculated By MD5
Text Format:
Number of Header Lines:1
Record Delimiter:\r\n
Orientation:column
Simple Delimited:
Field Delimiter:,
Quote Character:"

Table Column Descriptions
 speciesrepwater_treatdiv_treatsurvival_propdensity
Column Name:species  
rep  
water_treat  
div_treat  
survival_prop  
density  
Definition:Species of cover cropIdentity of each replicateWater stress treatment in the experimentDiversity treatment in the experimentProportion of plants survivingNumber of plants seeded
Storage Type:string  
string  
string  
string  
float  
float  
Measurement Type:nominalnominalnominalnominalratioratio
Measurement Values Domain:
Allowed Values and Definitions
Enumerated Domain 
Code Definition
CodeCrimson Clover
DefinitionCrimson clover (Trifolium incarnatum)
Source
Code Definition
CodeRye
DefinitionRye (Secale cereale)
Source
Allowed Values and Definitions
Enumerated Domain 
Code Definition
Code1
DefinitionFirst replicate
Source
Code Definition
Code2
DefinitionSecond replicate
Source
Code Definition
Code3
DefinitionThird replicate
Source
Code Definition
Code4
DefinitionFourth replicate
Source
Allowed Values and Definitions
Enumerated Domain 
Code Definition
CodeControl
DefinitionMaintained at 100% field capacity
Source
Code Definition
CodeDrought
DefinitionWithheld water for one week
Source
Code Definition
CodeWaterlogging
DefinitionLeft flooded at 5 mm above the surface for one week
Source
Allowed Values and Definitions
Enumerated Domain 
Code Definition
CodeMonoculture
DefinitionSingle species by itself
Source
Code Definition
CodePolyculture
DefinitionBiculture of rye and crimson clover
Source
Unitproportion
Typereal
Unitnumber
Typereal
Missing Value Code:            
Accuracy Report:            
Accuracy Assessment:            
Coverage:            
Methods:            

Data Table

Data:https://pasta-s.lternet.edu/package/data/eml/edi/1703/1/c75da32fd5ecf09cc67e2f61b1f2c3b9
Name:Crimson Clover's growth score
Description:The table provides daily growth score of crimson clover by replicate, water stress, species number, and phase (i.e., water treatment and recovery). Growth score was determined by the emergence of cotyledons, first true leaf, first trifoliate leaf, second trifoliate leaf, etc.; each stage was equivalent to one point.
Number of Records:288
Number of Columns:7

Table Structure
Object Name:score_cc.csv
Size:13212 byte
Authentication:bd0e9571133765a31d11f8a113b15535 Calculated By MD5
Text Format:
Number of Header Lines:1
Record Delimiter:\r\n
Orientation:column
Simple Delimited:
Field Delimiter:,
Quote Character:"

Table Column Descriptions
 date_iddatephaserepwater_treatdiv_treatgrowth_score
Column Name:date_id  
date  
phase  
rep  
water_treat  
div_treat  
growth_score  
Definition:Days since the start of the water stress treatment.DatePhase in the experiment (during water stress or recovery phase)Identity of each replicateWater stress treatment in the experimentDiversity treatment in the experimentMean growth score for crimson clover seedlings. Growth stages included emergence of cotyledons, first true leaf, first trifoliate leaf, second trifoliate leaf, etc., with each stage equivalent to one point.
Storage Type:float  
dateTime  
string  
string  
string  
string  
float  
Measurement Type:ratiodateTimenominalnominalnominalnominalratio
Measurement Values Domain:
Unitday
Typeinteger
FormatYYYY-MM-DD
Precision
Allowed Values and Definitions
Enumerated Domain 
Code Definition
CodeA
DefinitionDuring water stress
Source
Code Definition
CodeB
DefinitionDuring recovery period
Source
Allowed Values and Definitions
Enumerated Domain 
Code Definition
Code1
DefinitionFirst replicate
Source
Code Definition
Code2
DefinitionSecond replicate
Source
Code Definition
Code3
DefinitionThird replicate
Source
Code Definition
Code4
DefinitionFourth replicate
Source
Allowed Values and Definitions
Enumerated Domain 
Code Definition
Codecontrol
DefinitionMaintained at 100% field capacity
Source
Code Definition
Codedrought
DefinitionWithheld water for one week
Source
Code Definition
Codewaterlogging
DefinitionLeft flooded at 5 mm above the surface for one week
Source
Allowed Values and Definitions
Enumerated Domain 
Code Definition
Codemonoculture
DefinitionSingle species by itself
Source
Code Definition
Codepolyculture
DefinitionBiculture of rye and crimson clover
Source
Unitpoints
Typereal
Missing Value Code:              
Accuracy Report:              
Accuracy Assessment:              
Coverage:              
Methods:              

Data Table

Data:https://pasta-s.lternet.edu/package/data/eml/edi/1703/1/37090b97de8c333a965dd4b3b48ee03c
Name:Rye's growth score
Description:The table provides daily growth score of rye by replicate, water stress, species number, and phase (i.e., water treatment and recovery). One point of growth score indicated a visible leaf collar.
Number of Records:288
Number of Columns:7

Table Structure
Object Name:score_r.csv
Size:12699 byte
Authentication:e63090fb20a0191c661d714bff723c91 Calculated By MD5
Text Format:
Number of Header Lines:1
Record Delimiter:\r\n
Orientation:column
Simple Delimited:
Field Delimiter:,
Quote Character:"

Table Column Descriptions
 date_iddatePhaserepwater_treatdiv_treatgrowth_score
Column Name:date_id  
date  
Phase  
rep  
water_treat  
div_treat  
growth_score  
Definition:Days since the start of the water stress treatment.DatePhase in the experiment (during water stress or recovery phase)Identity of each replicateWater stress treatment in the experimentDiversity treatment in the experimentMean growth score for crimson clover seedlings. Growth stages included emergence of cotyledons, first true leaf, first trifoliate leaf, second trifoliate leaf, etc., with each stage equivalent to one point.
Storage Type:float  
dateTime  
string  
string  
string  
string  
float  
Measurement Type:ratiodateTimenominalnominalnominalnominalratio
Measurement Values Domain:
Unitday
Typeinteger
FormatYYYY-MM-DD
Precision
Allowed Values and Definitions
Enumerated Domain 
Code Definition
CodeA
DefinitionDuring water stress
Source
Code Definition
CodeB
DefinitionDuring recovery period
Source
Allowed Values and Definitions
Enumerated Domain 
Code Definition
Code1
DefinitionFirst replicate
Source
Code Definition
Code2
DefinitionSecond replicate
Source
Code Definition
Code3
DefinitionThird replicate
Source
Code Definition
Code4
DefinitionFourth replicate
Source
Allowed Values and Definitions
Enumerated Domain 
Code Definition
Codecontrol
DefinitionMaintained at 100% field capacity
Source
Code Definition
Codedrought
DefinitionWithheld water for one week
Source
Code Definition
Codewaterlogging
DefinitionLeft flooded at 5 mm above the surface for one week
Source
Allowed Values and Definitions
Enumerated Domain 
Code Definition
Codemonoculture
DefinitionSingle species by itself
Source
Code Definition
Codepolyculture
DefinitionBiculture of rye and crimson clover
Source
Unitpoints
Typereal
Missing Value Code:              
Accuracy Report:              
Accuracy Assessment:              
Coverage:              
Methods:              

Data Table

Data:https://pasta-s.lternet.edu/package/data/eml/edi/1703/1/1104ad8491e7b53d6aa242a366943661
Name:Crimson Clover's normalized root scan outputs
Description:This table contains the outputs of Crimson Clover root scans from RhizoVision Explorer after normalization by the number of plants per pot. Variables are organized by water stress, species number, replicate, number of plants per pot.
Number of Records:24
Number of Columns:19

Table Structure
Object Name:cc_scan_nor.csv
Size:3901 byte
Authentication:c631b0e15c83a5ca8c406415c172d9d7 Calculated By MD5
Text Format:
Number of Header Lines:1
Record Delimiter:\n
Orientation:column
Simple Delimited:
Field Delimiter:,
Quote Character:"

Table Column Descriptions
 repdiv_treatwater_treatnumX1X2X3X4X5X6X7X8X9X10X11X12X13X14treat
Column Name:rep  
div_treat  
water_treat  
num  
X1  
X2  
X3  
X4  
X5  
X6  
X7  
X8  
X9  
X10  
X11  
X12  
X13  
X14  
treat  
Definition:Identity of each replicateDiversity treatment in the experimentWater stress treatment in the experimentNumber of survived seedlings at the end of the experimentRoot:shoot mass ratioBiomass per plantNumber of root tips per plantTotal root length per plantBranching frequency per millimeter of root lengthAverage root diameterMaximum root diameterRoot length per plant of diameter range 0-0.25 mmRoot length per plant of diameter range 0.25-0.5 mmRoot length per plant of diameter range 0.5-1 mmRoot length per plant of diameter range 1-2 mmRoot length per plant where diameter is greater than 2 mmRoot length ratio of very fine to the entire rootRoot length ratio of very fine to fine rootTreatment code (water treatment + diversity treatment)
Storage Type:string  
string  
string  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
string  
Measurement Type:nominalnominalnominalratioratioratioratioratioratioratioratioratioratioratioratioratioratiorationominal
Measurement Values Domain:
Allowed Values and Definitions
Enumerated Domain 
Code Definition
Code1
DefinitionFirst replicate
Source
Code Definition
Code2
DefinitionSecond replicate
Source
Code Definition
Code3
DefinitionThird replicate
Source
Code Definition
Code4
DefinitionFourth replicate
Source
Allowed Values and Definitions
Enumerated Domain 
Code Definition
CodeM
DefinitionMonoculture; single species by itself
Source
Code Definition
CodeP
DefinitionPolyculture; biculture of rye and crimson clover
Source
Allowed Values and Definitions
Enumerated Domain 
Code Definition
CodeC
DefinitionControl; maintained at 100% field capacity
Source
Code Definition
CodeD
DefinitionDrought; withheld water for one week
Source
Code Definition
CodeW
DefinitionWaterlogging; left flooded at 5 mm above the surface for one week
Source
Unitplant
Typeinteger
UnitN/A
Typereal
Unitgram
Typereal
UnitTip pixel
Typereal
Unitmillimeter
Typereal
UnitNumber of branch points by millimeter
Typereal
Unitmillimeter
Typereal
Unitmillimeter
Typereal
Unitmillimeter
Typereal
Unitmillimeter
Typereal
Unitmillimeter
Typereal
Unitmillimeter
Typereal
Unitmillimeter
Typereal
UnitN/A
Typereal
UnitN/A
Typereal
Allowed Values and Definitions
Enumerated Domain 
Code Definition
CodeC + M
DefinitionControl + Monoculture
Source
Code Definition
CodeC + P
DefinitionControl + Polyculture
Source
Code Definition
CodeD + M
DefinitionDrought + Monoculture
Source
Code Definition
CodeD + P
DefinitionDrought + Polyculture
Source
Code Definition
CodeW + M
DefinitionWaterlogged + Monoculture
Source
Code Definition
CodeW + P
DefinitionWaterlogged + Polyculture
Source
Missing Value Code:                                      
Accuracy Report:                                      
Accuracy Assessment:                                      
Coverage:                                      
Methods:                                      

Data Table

Data:https://pasta-s.lternet.edu/package/data/eml/edi/1703/1/469efea6d6082daf919ea17720ff0a3e
Name:Rye's normalized root scan outputs
Description:This table contains the outputs of Rye root scans from RhizoVision Explorer after normalization by the number of plants per pot. Variables are organized by water stress, species number, replicate, number of plants per pot.
Number of Records:24
Number of Columns:19

Table Structure
Object Name:r_scan_nor.csv
Size:4232 byte
Authentication:83d5ad8ce765ed0b6d81f63ec11d8cce Calculated By MD5
Text Format:
Number of Header Lines:1
Record Delimiter:\n
Orientation:column
Simple Delimited:
Field Delimiter:,
Quote Character:"

Table Column Descriptions
 repdiv_treatwater_treatnumX1X2X3X4X5X6X7X8X9X10X11X12X13X14treat
Column Name:rep  
div_treat  
water_treat  
num  
X1  
X2  
X3  
X4  
X5  
X6  
X7  
X8  
X9  
X10  
X11  
X12  
X13  
X14  
treat  
Definition:Identity of each replicateDiversity treatment in the experimentWater stress treatment in the experimentNumber of survived seedlings at the end of the experimentRoot:shoot mass ratioBiomass per plantNumber of root tips per plantTotal root length per plantBranching frequency per millimeter of root lengthAverage root diameterMaximum root diameterRoot length per plant of diameter range 0-0.25 mmRoot length per plant of diameter range 0.25-0.5 mmRoot length per plant of diameter range 0.5-1 mmRoot length per plant of diameter range 1-2 mmRoot length per plant where diameter is greater than 2 mmRoot length ratio of very fine to the entire rootRoot length ratio of very fine to fine rootTreatment code (water treatment + diversity treatment)
Storage Type:string  
string  
string  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
string  
Measurement Type:nominalnominalnominalratioratioratioratioratioratioratioratioratioratioratioratioratioratiorationominal
Measurement Values Domain:
Allowed Values and Definitions
Enumerated Domain 
Code Definition
Code1
DefinitionFirst replicate
Source
Code Definition
Code2
DefinitionSecond replicate
Source
Code Definition
Code3
DefinitionThird replicate
Source
Code Definition
Code4
DefinitionFourth replicate
Source
Allowed Values and Definitions
Enumerated Domain 
Code Definition
CodeM
DefinitionMonoculture; single species by itself
Source
Code Definition
CodeP
DefinitionPolyculture; biculture of rye and crimson clover
Source
Allowed Values and Definitions
Enumerated Domain 
Code Definition
CodeC
DefinitionControl; maintained at 100% field capacity
Source
Code Definition
CodeD
DefinitionDrought; withheld water for one week
Source
Code Definition
CodeW
DefinitionWaterlogging; left flooded at 5 mm above the surface for one week
Source
Unitplant
Typereal
UnitN/A
Typereal
Unitgram
Typereal
UnitTip pixel
Typereal
Unitmillimeter
Typereal
UnitNumber of branch points by millimeter
Typereal
Unitmillimeter
Typereal
Unitmillimeter
Typereal
Unitmillimeter
Typereal
Unitmillimeter
Typereal
Unitmillimeter
Typereal
Unitmillimeter
Typereal
Unitmillimeter
Typereal
UnitN/A
Typereal
UnitN/A
Typereal
Allowed Values and Definitions
Enumerated Domain 
Code Definition
CodeC + M
DefinitionControl + Monoculture
Source
Code Definition
CodeC + P
DefinitionControl + Polyculture
Source
Code Definition
CodeD + M
DefinitionDrought + Monoculture
Source
Code Definition
CodeD + P
DefinitionDrought + Polyculture
Source
Code Definition
CodeW + M
DefinitionWaterlogged + Monoculture
Source
Code Definition
CodeW + P
DefinitionWaterlogged + Polyculture
Source
Missing Value Code:                                      
Accuracy Report:                                      
Accuracy Assessment:                                      
Coverage:                                      
Methods:                                      

Data Table

Data:https://pasta-s.lternet.edu/package/data/eml/edi/1703/1/ba048e0b27d908649ac0cc9a37b0fed1
Name:Crimson Clover's raw root scan outputs
Description:This table contains the outputs of Crimson Clover root scans from RhizoVision Explorer. Variables are organized by water stress, species number, replicate, number of plants per pot.
Number of Records:24
Number of Columns:18

Table Structure
Object Name:cc_scan_num.csv
Size:3340 byte
Authentication:ec313498bb6fe97cd5c2b102dfdce254 Calculated By MD5
Text Format:
Number of Header Lines:1
Record Delimiter:\r\n
Orientation:column
Simple Delimited:
Field Delimiter:,
Quote Character:"

Table Column Descriptions
 repdiv_treatwater_treatnumX1X2X3X4X5X6X7X8X9X10X11X12X13X14
Column Name:rep  
div_treat  
water_treat  
num  
X1  
X2  
X3  
X4  
X5  
X6  
X7  
X8  
X9  
X10  
X11  
X12  
X13  
X14  
Definition:Identity of each replicateDiversity treatment in the experimentWater stress treatment in the experimentNumber of survived seedlings at the end of the experimentRoot:shoot mass ratioBiomassNumber of root tipsTotal root length per plantBranching frequency per millimeter of root lengthAverage root diameterMaximum root diameterRoot length of diameter range 0-0.25 mmRoot length of diameter range 0.25-0.5 mmRoot length of diameter range 0.5-1 mmRoot length of diameter range 1-2 mmRoot length where diameter is greater than 2 mmRoot length ratio of very fine to the entire rootRoot length ratio of very fine to fine root
Storage Type:string  
string  
string  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
Measurement Type:nominalnominalnominalratioratioratioratioratioratioratioratioratioratioratioratioratioratioratio
Measurement Values Domain:
Allowed Values and Definitions
Enumerated Domain 
Code Definition
Code1
DefinitionFirst replicate
Source
Code Definition
Code2
DefinitionSecond replicate
Source
Code Definition
Code3
DefinitionThird replicate
Source
Code Definition
Code4
DefinitionFourth replicate
Source
Allowed Values and Definitions
Enumerated Domain 
Code Definition
CodeM
DefinitionMonoculture; single species by itself
Source
Code Definition
CodeP
DefinitionPolyculture; biculture of rye and crimson clover
Source
Allowed Values and Definitions
Enumerated Domain 
Code Definition
CodeC
DefinitionControl; maintained at 100% field capacity
Source
Code Definition
CodeD
DefinitionDrought; withheld water for one week
Source
Code Definition
CodeW
DefinitionWaterlogging; left flooded at 5 mm above the surface for one week
Source
Unitplant
Typeinteger
UnitN/A
Typereal
Unitgram
Typereal
UnitTip pixel
Typereal
Unitmillimeter
Typereal
UnitNumber of branch points by millimeter
Typereal
Unitmillimeter
Typereal
Unitmillimeter
Typereal
Unitmillimeter
Typereal
Unitmillimeter
Typereal
Unitmillimeter
Typereal
Unitmillimeter
Typereal
Unitmillimeter
Typereal
UnitN/A
Typereal
UnitN/A
Typereal
Missing Value Code:                                    
Accuracy Report:                                    
Accuracy Assessment:                                    
Coverage:                                    
Methods:                                    

Data Table

Data:https://pasta-s.lternet.edu/package/data/eml/edi/1703/1/992f878daa1ec271a273dbb4be71ba10
Name:Rye's raw root scan outputs
Description:This table contains the outputs of Rye root scans from RhizoVision Explorer. Variables are organized by water stress, species number, replicate, number of plants per pot.
Number of Records:24
Number of Columns:18

Table Structure
Object Name:r_scan_num.csv
Size:3563 byte
Authentication:0a25ab0c29a2ac815321ef8302f33890 Calculated By MD5
Text Format:
Number of Header Lines:1
Record Delimiter:\r\n
Orientation:column
Simple Delimited:
Field Delimiter:,
Quote Character:"

Table Column Descriptions
 repdiv_treatwater_treatnumX1X2X3X4X5X6X7X8X9X10X11X12X13X14
Column Name:rep  
div_treat  
water_treat  
num  
X1  
X2  
X3  
X4  
X5  
X6  
X7  
X8  
X9  
X10  
X11  
X12  
X13  
X14  
Definition:Identity of each replicateDiversity treatment in the experimentWater stress treatment in the experimentNumber of survived seedlings at the end of the experimentRoot:shoot mass ratioBiomassNumber of root tipsTotal root lengthBranching frequency per millimeter of root lengthAverage root diameterMaximum root diameterRoot length of diameter range 0-0.25 mmRoot length of diameter range 0.25-0.5 mmRoot length of diameter range 0.5-1 mmRoot length of diameter range 1-2 mmRoot length where diameter is greater than 2 mmRoot length ratio of very fine to the entire rootRoot length ratio of very fine to fine root
Storage Type:string  
string  
string  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
Measurement Type:nominalnominalnominalratioratioratioratioratioratioratioratioratioratioratioratioratioratioratio
Measurement Values Domain:
Allowed Values and Definitions
Enumerated Domain 
Code Definition
Code1
DefinitionFirst replicate
Source
Code Definition
Code2
DefinitionSecond replicate
Source
Code Definition
Code3
DefinitionThird replicate
Source
Code Definition
Code4
DefinitionFourth replicate
Source
Allowed Values and Definitions
Enumerated Domain 
Code Definition
CodeM
DefinitionMonoculture; single species by itself
Source
Code Definition
CodeP
DefinitionPolyculture; biculture of rye and crimson clover
Source
Allowed Values and Definitions
Enumerated Domain 
Code Definition
CodeC
DefinitionControl; maintained at 100% field capacity
Source
Code Definition
CodeD
DefinitionDrought; withheld water for one week
Source
Code Definition
CodeW
DefinitionWaterlogging; left flooded at 5 mm above the surface for one week
Source
Unitplant
Typereal
UnitN/A
Typereal
Unitgram
Typereal
UnitTip pixel
Typereal
Unitmillimeter
Typereal
UnitNumber of branch points by millimeter
Typereal
Unitmillimeter
Typereal
Unitmillimeter
Typereal
Unitmillimeter
Typereal
Unitmillimeter
Typereal
Unitmillimeter
Typereal
Unitmillimeter
Typereal
Unitmillimeter
Typereal
UnitN/A
Typereal
UnitN/A
Typereal
Missing Value Code:                                    
Accuracy Report:                                    
Accuracy Assessment:                                    
Coverage:                                    
Methods:                                    

Non-Categorized Data Resource

Name:dryweight_cc_anova
Entity Type:.R
Description:Code to analyze the effect of water stress treatment, species number, and their interaction on per-plant dry biomass of crimson clover seedlings.
Physical Structure Description:
Object Name:dryweight_cc_anova.R
Size:3440 byte
Authentication:c00a873592d08eed7bc50b740d75b37d Calculated By MD5
Externally Defined Format:
Format Name:.R
Data:https://pasta-s.lternet.edu/package/data/eml/edi/1703/1/6a1b5b104cea8e2310c33c486aad07c5

Non-Categorized Data Resource

Name:dryweight_r_glm
Entity Type:.R
Description:Code to analyze the effect of water stress treatment, species number, and their interaction on dry biomass of rye seedlings.
Physical Structure Description:
Object Name:dryweight_r_glm.R
Size:3041 byte
Authentication:a13da9497071c6cb94baaac71a27aef4 Calculated By MD5
Externally Defined Format:
Format Name:.R
Data:https://pasta-s.lternet.edu/package/data/eml/edi/1703/1/4374ab715d214ee509afd85aa7005896

Non-Categorized Data Resource

Name:dryweight_totalbiomass
Entity Type:.R
Description:Code to analyze the effect of water stress treatment, species number, and their interaction on total biomass of crimson clover and rye grown separately or together.
Physical Structure Description:
Object Name:dryweight_totalbiomass.R
Size:3969 byte
Authentication:60ceb832106e5bd2a08879b26a352b34 Calculated By MD5
Externally Defined Format:
Format Name:.R
Data:https://pasta-s.lternet.edu/package/data/eml/edi/1703/1/efd05bc52f4dd11f4146e6eb772150e6

Non-Categorized Data Resource

Name:growth_rate
Entity Type:.R
Description:Code to analyze the effect of water stress treatment, species number, and their interaction on growth rate of crimson clover and rye.
Physical Structure Description:
Object Name:growth_rate.R
Size:9890 byte
Authentication:ac735131cd8710654f72718fb4a6b069 Calculated By MD5
Externally Defined Format:
Format Name:.R
Data:https://pasta-s.lternet.edu/package/data/eml/edi/1703/1/ad2c816f95af2d39094613011f36311c

Non-Categorized Data Resource

Name:root_PCA_v2
Entity Type:.R
Description:Code for a principal component analysis to understand the influence of water stress and species number on the root morphology of crimson clover and rye.
Physical Structure Description:
Object Name:root_PCA_v2.R
Size:12591 byte
Authentication:69383cfb57e00ab36ae99fc0aaf38beb Calculated By MD5
Externally Defined Format:
Format Name:.R
Data:https://pasta-s.lternet.edu/package/data/eml/edi/1703/1/4daa291959708325097c2618813774ab

Non-Categorized Data Resource

Name:root_analysis
Entity Type:.R
Description:Code to analyze the effect of water stress treatment, species number, and their interaction on key root traits in crimson clover and rye. Traits included total root length (both species), average root diameter (crimson clover), and maximum root diameter (rye).
Physical Structure Description:
Object Name:root_analysis.R
Size:10607 byte
Authentication:0ea8a248e4ec863a2c320092c2e3d333 Calculated By MD5
Externally Defined Format:
Format Name:.R
Data:https://pasta-s.lternet.edu/package/data/eml/edi/1703/1/29aafbdd8642bd987972fcc4e69e84fe

Non-Categorized Data Resource

Name:survival_rate
Entity Type:.R
Description:Code to analyze the effect of water stress treatment, species number, and their interaction on seedling survival of crimson clover and rye.
Physical Structure Description:
Object Name:survival_rate.R
Size:4700 byte
Authentication:4a9c8400f4decb0a3e9eb2c8bc7aeb81 Calculated By MD5
Externally Defined Format:
Format Name:.R
Data:https://pasta-s.lternet.edu/package/data/eml/edi/1703/1/69041c49cfc731ab332e5d5807fb5da5

Data Package Usage Rights

This information is released under the Creative Commons license - Attribution - CC BY (https://creativecommons.org/licenses/by/4.0/). The consumer of these data ("Data User" herein) is required to cite it appropriately in any publication that results from its use. The Data User should realize that these data may be actively used by others for ongoing research and that coordination may be necessary to prevent duplicate publication. The Data User is urged to contact the authors of these data if any questions about methodology or results occur. Where appropriate, the Data User is encouraged to consider collaboration or co-authorship with the authors. The Data User should realize that misinterpretation of data may occur if used out of context of the original study. While substantial efforts are made to ensure the accuracy of data and associated documentation, complete accuracy of data sets cannot be guaranteed. All data are made available "as is." The Data User should be aware, however, that data are updated periodically and it is the responsibility of the Data User to check for new versions of the data. The data authors and the repository where these data were obtained shall not be liable for damages resulting from any use or misinterpretation of the data. Thank you.

Keywords

By Thesaurus:
LTER Controlled Vocabularybiodiversity, climate change, agriculture, plant growth, species interactions, biomass, roots, precipitation

Methods and Protocols

These methods, instrumentation and/or protocols apply to all data in this dataset:

Methods and protocols used in the collection of this data package
Description:

For a full description of the methods used to generate these data, please see: Truong, N.Q., York, L.M., Decker, A. and Douglas, M.R. (2024) A mixture of grass-legume cover crop species may ameliorate water stress in a changing climate. AoB Plants.

People and Organizations

Publishers:
Organization:Environmental Data Initiative
Email Address:
info@edirepository.org
Web Address:
https://edirepository.org
Id:https://ror.org/0330j0z60
Creators:
Individual: Nhu Quynh Truong
Organization:Unravel Carbon Pte Ltd
Address:
Ho Chi Minh City, Vietnam
Email Address:
truongquynhnhu0803@gmail.com
Individual: Larry M. York
Organization:Oak Ridge National Laboratory
Email Address:
yorklm@ornl.gov
Id:https://orcid.org/0000-0002-1995-9479
Individual: Allyssa Decker
Organization:Dickinson College
Email Address:
deckeral@dickinson.edu
Individual: Margaret R. Douglas
Organization:Dickinson College
Email Address:
douglasm@dickinson.edu
Id:https://orcid.org/0000-0001-5160-4822
Contacts:
Individual: Nhu Truong
Organization:Unravel Carbon Pte Ltd
Address:
Ho Chi Minh City, Vietnam
Email Address:
truongquynhnhu0803@gmail.com
Individual: Margaret R. Douglas
Organization:Dickinson College
Email Address:
douglasm@dickinson.edu
Id:https://orcid.org/0000-0001-5160-4822

Temporal, Geographic and Taxonomic Coverage

Temporal, Geographic and/or Taxonomic information that applies to all data in this dataset:

Time Period
Begin:
2021-06-01
End:
2021-07-16
Sampling Site: 
Description:The data described here were generated in a greenhouse experiment at Dickinson College in Carlisle, PA, USA.
Site Coordinates:
Longitude (degree): -77.2008Latitude (degree): 40.2027
Taxonomic Range:
Classification:
Rank Name:Kingdom
Rank Value:Plantae
Common Name:plants
Identifer:https://www.itis.gov
ID: 202422
Classification:
Rank Name:Subkingdom
Rank Value:Viridiplantae
Common Name:green plants
Identifer:https://www.itis.gov
ID: 954898
Classification:
Rank Name:Infrakingdom
Rank Value:Streptophyta
Common Name:land plants
Identifer:https://www.itis.gov
ID: 846494
Classification:
Rank Name:Division
Rank Value:Tracheophyta
Common Name:vascular plants
Identifer:https://www.itis.gov
ID: 846496
Classification:
Rank Name:Subdivision
Rank Value:Spermatophytina
Common Name:spermatophytes
Identifer:https://www.itis.gov
ID: 846504
Classification:
Rank Name:Class
Rank Value:Magnoliopsida
Identifer:https://www.itis.gov
ID: 18063
Classification:
Rank Name:Superorder
Rank Value:Lilianae
Common Name:monocots
Identifer:https://www.itis.gov
ID: 846542
Classification:
Rank Name:Order
Rank Value:Poales
Identifer:https://www.itis.gov
ID: 846620
Classification:
Rank Name:Family
Rank Value:Poaceae
Identifer:https://www.itis.gov
ID: 40351
Classification:
Rank Name:Genus
Rank Value:Secale
Identifer:https://www.itis.gov
ID: 42089
Classification:
Rank Name:Species
Rank Value:Secale cereale
Common Name:cultivated annual rye
Identifer:https://www.itis.gov
ID: 42090
Taxonomic Range:
Classification:
Rank Name:Kingdom
Rank Value:Plantae
Common Name:plants
Identifer:https://www.itis.gov
ID: 202422
Classification:
Rank Name:Subkingdom
Rank Value:Viridiplantae
Common Name:green plants
Identifer:https://www.itis.gov
ID: 954898
Classification:
Rank Name:Infrakingdom
Rank Value:Streptophyta
Common Name:land plants
Identifer:https://www.itis.gov
ID: 846494
Classification:
Rank Name:Division
Rank Value:Tracheophyta
Common Name:vascular plants
Identifer:https://www.itis.gov
ID: 846496
Classification:
Rank Name:Subdivision
Rank Value:Spermatophytina
Common Name:spermatophytes
Identifer:https://www.itis.gov
ID: 846504
Classification:
Rank Name:Class
Rank Value:Magnoliopsida
Identifer:https://www.itis.gov
ID: 18063
Classification:
Rank Name:Superorder
Rank Value:Rosanae
Identifer:https://www.itis.gov
ID: 846548
Classification:
Rank Name:Order
Rank Value:Fabales
Identifer:https://www.itis.gov
ID: 500022
Classification:
Rank Name:Family
Rank Value:Fabaceae
Common Name:peas
Identifer:https://www.itis.gov
ID: 500059
Classification:
Rank Name:Genus
Rank Value:Trifolium
Common Name:clover
Identifer:https://www.itis.gov
ID: 26204
Classification:
Rank Name:Species
Rank Value:Trifolium incarnatum
Common Name:crimson clover
Identifer:https://www.itis.gov
ID: 26262

Project

Parent Project Information:

Title:A mixture of grass-legume cover crop species may ameliorate water stress in a changing climate
Personnel:
Individual: Nhu Q. Truong
Organization:Unravel Carbon Pte. Ltd.
Email Address:
truongquynhnhu0803@gmail.com
Role:Lead investigator
Individual: Larry M. York
Organization:Oak Ridge National Laboratory
Email Address:
yorklm@ornl.gov
Id:https://orcid.org/0000-0002-1995-9479
Role:Collaborator
Individual: Allyssa Decker
Organization:Dickinson College
Email Address:
deckeral@dickinson.edu
Role:Collaborator
Individual: Margaret R. Douglas
Organization:Dickinson College
Email Address:
douglasm@dickinson.edu
Id:https://orcid.org/0000-0001-5160-4822
Role:Senior investigator
Abstract:

Climate change models predict increasing precipitation variability in the mid-latitude regions of Earth, generating a need to reduce negative impacts of these changes on crop production. Despite considerable research on how cover crops support agriculture in a changing climate, understanding is limited of how climate change influences the growth of cover crops. We investigated the early development of two common cover crop species – crimson clover (Trifolium incarnatum L.) and rye (Secale cereale L.) – and hypothesized that growing them in mixture would ameliorate stress from drought or waterlogging. This hypothesis was tested in a 25-day greenhouse experiment, where the two factors (species number, water stress) were fully crossed in randomized blocks, and plant responses were quantified through survival, growth rate, biomass production, and root morphology. Water stress negatively influenced the early growth of these two species in contrasting ways: crimson clover was susceptible to drought while rye performed poorly under waterlogging. Per-plant biomass in rye was always greater in mixture than in monoculture, while per-plant biomass of crimson clover was greater in mixture under drought. Both species grew longer roots in mixture than in monoculture under drought, and total biomass of mixtures did not differ significantly from the more-productive monoculture (rye) in any water condition. In the face of increasingly variable precipitation, growing crimson clover and rye together has potential to ameliorate water stress, a possibility that should be further investigated in field experiments.

Additional Award Information:
Funder:Dickinson Research & Development Committee
Title:Robert Allan Jansen Memorial Student-Faculty Research Fund
Additional Award Information:
Funder:US Department of Energy (DOE)
Title:DE-AC05-00OR22725

Maintenance

Maintenance:
Description:

This dataset and code are associated with a published paper, so maintenance is not planned.

Frequency:notPlanned
Other Metadata

Additional Metadata

additionalMetadata
        |___text '\n      '
        |___element 'metadata'
        |     |___text '\n         '
        |     |___element 'unitList'
        |     |     |___text '\n            '
        |     |     |___element 'unit'
        |     |     |     |  \___attribute 'id' = 'proportion'
        |     |     |     |  \___attribute 'name' = 'proportion'
        |     |     |     |___text '\n               '
        |     |     |     |___element 'description'
        |     |     |     |___text '\n            '
        |     |     |___text '\n            '
        |     |     |___element 'unit'
        |     |     |     |  \___attribute 'id' = 'day'
        |     |     |     |  \___attribute 'name' = 'day'
        |     |     |     |___text '\n               '
        |     |     |     |___element 'description'
        |     |     |     |___text '\n            '
        |     |     |___text '\n            '
        |     |     |___element 'unit'
        |     |     |     |  \___attribute 'id' = 'points'
        |     |     |     |  \___attribute 'name' = 'points'
        |     |     |     |___text '\n               '
        |     |     |     |___element 'description'
        |     |     |     |___text '\n            '
        |     |     |___text '\n            '
        |     |     |___element 'unit'
        |     |     |     |  \___attribute 'id' = 'plant'
        |     |     |     |  \___attribute 'name' = 'plant'
        |     |     |     |___text '\n               '
        |     |     |     |___element 'description'
        |     |     |     |     |___text 'Number of plants in the pot by the end of the experiment'
        |     |     |     |___text '\n            '
        |     |     |___text '\n            '
        |     |     |___element 'unit'
        |     |     |     |  \___attribute 'id' = 'N/A'
        |     |     |     |  \___attribute 'name' = 'N/A'
        |     |     |     |___text '\n               '
        |     |     |     |___element 'description'
        |     |     |     |     |___text 'The variable was calculated by dividing the root length of very fine root to the total root length'
        |     |     |     |___text '\n            '
        |     |     |___text '\n            '
        |     |     |___element 'unit'
        |     |     |     |  \___attribute 'id' = 'Number of branch points by millimeter'
        |     |     |     |  \___attribute 'name' = 'Number of branch points by millimeter'
        |     |     |     |___text '\n               '
        |     |     |     |___element 'description'
        |     |     |     |     |___text 'The variable was calculated by dividing number of branch points by the total root length'
        |     |     |     |___text '\n            '
        |     |     |___text '\n            '
        |     |     |___element 'unit'
        |     |     |     |  \___attribute 'id' = 'Tip pixel'
        |     |     |     |  \___attribute 'name' = 'Tip pixel'
        |     |     |     |___text '\n               '
        |     |     |     |___element 'description'
        |     |     |     |     |___text 'The variable was computed by counting the total number of tip pixels in the skeletonized image with topology and dividing by number of plants in the replicate'
        |     |     |     |___text '\n            '
        |     |     |___text '\n         '
        |     |___text '\n      '
        |___text '\n   '

Additional Metadata

additionalMetadata
        |___text '\n      '
        |___element 'metadata'
        |     |___text '\n         '
        |     |___element 'emlEditor'
        |     |        \___attribute 'app' = 'ezEML'
        |     |        \___attribute 'release' = '2024.05.22'
        |     |___text '\n      '
        |___text '\n   '

EDI is a collaboration between the University of New Mexico and the University of Wisconsin – Madison, Center for Limnology:

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