Data Package Metadata View Summary

Protist Dispersal Detection: University of Michigan Biological Station, July 2024

General Information

Data Package:

Local Identifier:

edi.1800.1

Title:

Protist Dispersal Detection: University of Michigan Biological Station, July 2024

Alternate Identifier:

DOI PLACE HOLDER

Abstract:

This dataset contains the results of a field dispersal array assembled in Gates Bog, Pellston, Michigan. The data were collected by a graduate student, and consist of measurements of protist presence or absence in 1mL fluid samples taken from pitcher plants and centrifuge tubes in the array. The dataset contains both initial protist detection from the fluid samples, as well as detection after a 24 hour incubation period. The dataset also contains the positions of each plant and tube used for sample collection and their distances from the established source population at the center of the array. We used the purple pitcher plant, Sarracenia purpurea, as a model system to explore questions of specialist protist dispersal. Newly opened pitchers are sterile, providing virgin habitat open to community assembly of highly specialized protist species (Peterson 2008). The placement of a known community of protists at the center of an uncolonized array of habitat patches allows us to identify both sources and destinations of dispersing microbes in the array. The purpose of this study is to measure dispersal rates for a subset of pitcher plant protist species.

Publication Date:

2024-11-14

For more information:
Visit:	DOI PLACE HOLDER

Time Period

Begin:

2024-07-21

End:

2024-07-28

People and Organizations
Contact:	Burroughs, Nicole (North Carolina State University, PhD Student) [ email ]
Contact:	Petry, WIll (North Carolina State University, PI) [ email ]
Creator:	Burroughs, Nicole (North Carolina State University, PhD Student)
Associate:	Tallant, Jason M (University of Michigan Biological Station, Data Manager, Data Manager)
Associate:	Habicht, Helen (University of Michigan Biological Station, Research Coordinator and Lab Manager, Data Submission Review)

Data Entities
Data Table Name:	protist _detection_array - Sheet1
Description:	2024 Field array sampling points, distances, and protist invasion detection
Other Name:	Dispersal Array Field Setup
Description:	Visual layout of field experiment after adjustments to accommodate unforeseen restrictions of field site and plant health

Detailed Metadata

Data Entities

Data Table


Data:	https://pasta-s.lternet.edu/package/data/eml/edi/1800/1/7b39b5d28b93b9144fd8e99a225af568
Name:	protist _detection_array - Sheet1
Description:	2024 Field array sampling points, distances, and protist invasion detection
Number of Records:	733
Number of Columns:	12

Table Structure

Object Name:

protist _detection_array - Sheet1.csv

Size:

45866 byte

Authentication:

be2aa998d24b98c9a7022f5c66d845c4 Calculated By MD5

Text Format:

Number of Header Lines:

Record Delimiter:

\r\n

Orientation:

column

Simple Delimited:

Field Delimiter:	,
Quote Character:	"

Table Column Descriptions

Date

Measurement

Time

distance_m

Plant

Pitcher_Tube

initial_pres

initial_reset

24h_pres

24h_reset

Activity

Notes

Column Name:

date

measurement

time

distance_m

plant

pitcher_tube

initial_pres

initial_reset

24h_pres

24h_reset

activity

Notes

Definition:

date of initial sampling period

order of measurements

time of day when sampling began

distance from the center of the array

short hand code for each individual pitcher and tube, see position codes table for sampling point locations

distinguishes between pitchers on a single plant and plant or tube data

indicator of whether protists were detected at the time of sampling

indicator of whether the pitcher or tube was 'reset' following experimental sterilization protocol immediately after sampling

indicator of whether protists were detected 24 hours after the initial collection of the sample

indicator of whether the pitcher or tube was 'reset' following experimental sterilization protocol 24 hours after sampling

indicates whether the pitcher or tube was being sampled at the time of measurement, also indicates whether the pitcher or tube was usable as a sampling location

additional notes

Storage Type:

dateTime

float

string

float

string

Measurement Type:

dateTime

ratio

nominal

ratio

nominal

Measurement Values Domain:

Format	YYYY-MM-DD
Precision

Unit	measurement number
Type	real

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	17:55:00
Definition	time of day
Source

Code Definition

Code	18:06:00
Definition	time of day
Source

Code Definition

Code	18:10:00
Definition	time of day
Source

Code Definition

Code	18:18:00
Definition	time of day
Source

Code Definition

Code	18:36:00
Definition	time of day
Source

Code Definition

Code	18:40:00
Definition	time of day
Source

Code Definition

Code	19:08:00
Definition	time of day
Source

Code Definition

Code	8:42:00
Definition	time of day
Source

Code Definition

Code	8:56:00
Definition	time of day
Source

Code Definition

Code	8:58:00
Definition	time of day
Source

Code Definition

Code	9:04:00
Definition	time of day
Source

Code Definition

Code	9:05:00
Definition	time of day
Source

Code Definition

Code	9:13:00
Definition	time of day
Source

Unit	meter
Precision	.01
Type	real

Definition

text

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	A
Definition	sample from the larger or only pitcher on a plant
Source

Code Definition

Code	B
Definition	sample from a smaller (secondary) pitcher on a single plant
Source

Code Definition

Code	T
Definition	sample was taken from a tube
Source

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	FALSE
Definition	protists not detected
Source

Code Definition

Code	TRUE
Definition	protists detected
Source

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	FALSE
Definition	pitcher/tube was not sterilized and refilled with media and bacteria
Source

Code Definition

Code	TRUE
Definition	pitcher/tube sterilized and refilled with media and bacteria
Source

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	FALSE
Definition	protists not detected
Source

Code Definition

Code	TRUE
Definition	protists detected
Source

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	FALSE
Definition	pitcher/tube was not sterilized and refilled with media and bacteria
Source

Code Definition

Code	TRUE
Definition	pitcher/tube sterilized and refilled with media and bacteria
Source

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	Active
Definition	usable as a sampling location, sampled
Source

Code Definition

Code	Active_Not_Sampled
Definition	usable as a sampling location, not sampled
Source

Code Definition

Code	Inactive
Definition	not usable as a sampling location
Source

Definition

text

Missing Value Code:

Code	nan
Expl	end of datasheet

Accuracy Report:

Accuracy Assessment:

Coverage:

Methods:

Non-Categorized Data Resource

Name:

Dispersal Array Field Setup

Entity Type:

image/png

Description:

Visual layout of field experiment after adjustments to accommodate unforeseen restrictions of field site and plant health

Physical Structure Description:

Object Name:

Dispersal Array Field Setup.png

Size:

105158 byte

Authentication:

3fdc8fd8d9665baf4173227b4aceb93c Calculated By MD5

Externally Defined Format:

Format Name:

image/png

Data:

https://pasta-s.lternet.edu/package/data/eml/edi/1800/1/fcdeafaa6fe042b3fa507f815ea3c93d

Data Package Usage Rights

This information is released under the Creative Commons license - Attribution - CC BY (https://creativecommons.org/licenses/by/4.0/). The consumer of these data ("Data User" herein) is required to cite it appropriately in any publication that results from its use. The Data User should realize that these data may be actively used by others for ongoing research and that coordination may be necessary to prevent duplicate publication. The Data User is urged to contact the authors of these data if any questions about methodology or results occur. Where appropriate, the Data User is encouraged to consider collaboration or co-authorship with the authors. The Data User should realize that misinterpretation of data may occur if used out of context of the original study. While substantial efforts are made to ensure the accuracy of data and associated documentation, complete accuracy of data sets cannot be guaranteed. All data are made available "as is." The Data User should be aware, however, that data are updated periodically and it is the responsibility of the Data User to check for new versions of the data. The data authors and the repository where these data were obtained shall not be liable for damages resulting from any use or misinterpretation of the data. Thank you.

Keywords

By Thesaurus:
(No thesaurus)	University of Michigan Biologial Station, sarracenia, bog, UMBS
LTER Controlled Vocabulary	biodiversity, colonization, community dynamics, community composition, dispersal, ecology, protozoa

Methods and Protocols

These methods, instrumentation and/or protocols apply to all data in this dataset:

Methods and protocols used in the collection of this data package

Description:

We were able to construct the array in the proposed field location (Gates bog- Lat: 45.591739, Lon: -84.671767) using 25 plants and 29, 50mL centrifuge tubes mimicking the fluid retention and size of the pitcher environment. The final array was 18m in diameter at its widest points (N, S, E, W) and 14.5m on the minor axes (NE, NW, SE, SW). A diagram of the array setup is available in the 'Other Entities' section of this record. We inoculated 3 central plants with our focal protist species following a randomized block design on the morning of Monday, July 22nd and, and monitored for invasion success in the surrounding protist-free plants. Our design requires rapid detection of newly arriving protists, however new arrivals will inherently be at very low concentration which may be below the detection threshold for up to 24 hours. We used the following two-step screening process to ensure the detection of all protist arrivals.

We visited the array twice daily, sampling from one to two flagged pitchers per plant, as well as 50mL tubes. During each visit, we drew a 1mL sample, replaced the lost volume with fresh media, and returned to the lab to screen samples for protists. In the lab we examined each sample under a microscope. If we detected protists, we mounted 100uL on an acrylic slide and preserved the remainder of the sample for qPCR species determination. To prevent dispersal from non-source pitchers/tubes, we returned to the field as quickly as possible to sterilize (using >60C media) and refill the pitcher or tube with protist-free media. If no protists were detected in the initial screening, the sample was incubated for 24h in the laboratory at 21°C and re-screened. We followed the same pitcher-sterilization procedure as before if the incubated samples contained protists, and recorded 'no dispersal events' if no protists were detected. This sampling regime concluded Sunday evening: July 28th.

Description:

References:

Peterson, C. N., Day, S., Wolfe, B. E., Ellison, A. M., Kolter, R., & Pringle, A. (2008). A keystone predator controls bacterial diversity in the pitcher‐plant (sarracenia purpurea) microecosystem. Environmental Microbiology, 10(9), 2257–2266. https://doi.org/10.1111/j.1462-2920.2008.01648.x

People and Organizations

Publishers:

Organization:

Environmental Data Initiative

Email Address:

info@edirepository.org

Web Address:

https://edirepository.org

Id:

https://ror.org/0330j0z60

Creators:

Individual:

Nicole Burroughs

Organization:

North Carolina State University

Position:

PhD Student

Address:

126 Marianna Pl,

Garner, NC 27529 United States

Phone:

9194484990 (voice)

Email Address:

nlesch@ncsu.edu

Contacts:

Individual:

Nicole Burroughs

Organization:

North Carolina State University

Position:

PhD Student

Address:

126 Marianna Pl,

Garner, NC 27529 United States

Phone:

9194484990 (voice)

Email Address:

nlesch@ncsu.edu

Individual:

WIll Petry

Organization:

North Carolina State University

Position:

Email Address:

wpetry@ncsu.edu

Associated Parties:

Individual:

Jason M Tallant

Organization:

University of Michigan Biological Station

Position:

Data Manager

Address:

9133 Biological Road,

Pellston, MI 49769 United States

Phone:

734-615-5005 (voice)

Email Address:

umbsdata@umich.edu

Web Address:

https://lsa.umich.edu/umbs/people/staff/jtallant.html

Id:

https://orcid.org/0000-0003-4790-8772

Id:

https://ror.org/02hhndj92

Role:

Data Manager

Individual:

Helen Habicht

Organization:

University of Michigan Biological Station

Position:

Research Coordinator and Lab Manager

Address:

9133 Biological Road,

Pellston, MI 49769 United States

Email Address:

habicht@umich.edu

Web Address:

https://lsa.umich.edu/umbs

Id:

https://orcid.org/0000-0002-2006-9422

Id:

https://ror.org/02hhndj92

Role:

Data Submission Review

Temporal, Geographic and Taxonomic Coverage

Temporal, Geographic and/or Taxonomic information that applies to all data in this dataset:

Time Period

Begin:

2024-07-21

End:

2024-07-28

Geographic Region:

Description:

Gates Bog, Pellston, Michigan

Bounding Coordinates:

Northern:	45.596119469217925	Southern:	45.5933042389691
Western:	-84.68230684485133	Eastern:	-84.67923839779161

Project

Parent Project Information:

Title:

Testing the effect of species dispersal differences on microbial community assembly

Personnel:

Individual:

Nicole Burroughs

Organization:

North Carolina State University

Address:

126 Marianna Pl,

Garner, NC 27529 United States

Phone:

9194484990 (voice)

Email Address:

nlesch@ncsu.edu

Role:

Project Lead

Individual:

Will Petry

Organization:

North Carolina State University

Email Address:

wpetry@ncsu.edu

Role:

Additional Award Information:

Funder:	UMBS
Title:	UMBS Graduate Research Fellowship

Additional Award Information:

Funder:	Michigan Biological Foundation
Title:	Michigan Biological Foundation Research Grant
URL:	https://michiganbotanicalsociety.org/mbf-grant-guidelines

Additional Award Information:

Funder:	Florence Hanes Trust
Title:	Hanes Fund
URL:	https://hanesmemorialfund.org/

Additional Award Information:

Funder:	North Carolina Native Plant Society
Title:	Tom and Bruce Shinn Grant Award
URL:	https://ncwildflower.org/shinn-fund-recipients/

Maintenance

Maintenance:

Description:	All data collected on paper datasheets were scanned at the end of each day. All raw digital files are stored on the Petry Lab’s research cloud provided by North Carolina State University on Google Drive. A copy of the transcribed spreadsheet uploaded in this report will be stored with the raw digital files as well. Assuming continued funds are available for a second field season, this dataset will be updated in Sept. 2025 with a second week of array measurements in a similar format.
Frequency:

Other Metadata

Additional Metadata

additionalMetadata
        |___text '\n      '
        |___element 'metadata'
        |     |___text '\n         '
        |     |___element 'importedFromXML'
        |     |        \___attribute 'dateImported' = '2023-11-10'
        |     |        \___attribute 'filename' = 'UMBS_ Data Package Template.xml'
        |     |        \___attribute 'taxonomicCoverageExempt' = 'True'
        |     |___text '\n      '
        |___text '\n   '

Additional Metadata

additionalMetadata
        |___text '\n      '
        |___element 'metadata'
        |     |___text '\n         '
        |     |___element 'unitList' in ns 'http://www.xml-cml.org/schema/stmml-1.2' ('stmml:unitList')
        |     |     |___text '\n            '
        |     |     |___element 'unit' in ns 'http://www.xml-cml.org/schema/stmml-1.2' ('stmml:unit')
        |     |     |     |  \___attribute 'id' = 'measurement number'
        |     |     |     |  \___attribute 'name' = 'measurement number'
        |     |     |     |___text '\n               '
        |     |     |     |___element 'description' in ns 'http://www.xml-cml.org/schema/stmml-1.2' ('stmml:description')
        |     |     |     |     |___text 'order number of measurements taken during course of the experiment'
        |     |     |     |___text '\n            '
        |     |     |___text '\n         '
        |     |___text '\n      '
        |___text '\n   '

Additional Metadata

additionalMetadata
        |___text '\n      '
        |___element 'metadata'
        |     |___text '\n         '
        |     |___element 'emlEditor'
        |     |        \___attribute 'app' = 'ezEML'
        |     |        \___attribute 'release' = '2024.10.30'
        |     |___text '\n      '
        |___text '\n   '

Copyright 2024 Environmental Data Initiative. This material is based upon work supported by the National Science Foundation under grants #2223103 and #2223104. Any opinions, findings, conclusions, or recommendations expressed in the material are those of the author(s) and do not necessarily reflect the views of the National Science Foundation. Please contact us with questions, comments, or for technical assistance regarding this web site or the Environmental Data Initiative. Please read our privacy policy to know what information we collect about you and to understand your privacy rights.

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Data Package Metadata View Summary

Protist Dispersal Detection: University of Michigan Biological Station, July 2024

Data Entities

Data Table

Non-Categorized Data Resource

Data Package Usage Rights

Keywords

Methods and Protocols

These methods, instrumentation and/or protocols apply to all data in this dataset:

People and Organizations

Temporal, Geographic and Taxonomic Coverage

Project

Parent Project Information:

Maintenance

Additional Metadata

Additional Metadata

Additional Metadata

Recently Added

Recently Updated

Data Package Metadata View Summary

Protist Dispersal Detection: University of Michigan Biological Station, July 2024

+/- Data Entities

Data Table

Non-Categorized Data Resource

+/- Data Package Usage Rights

+/- Keywords

+/- Methods and Protocols

These methods, instrumentation and/or protocols apply to all data in this dataset:

+/- People and Organizations

+/- Temporal, Geographic and Taxonomic Coverage

+/- Project

Parent Project Information:

+/- Maintenance

+/- Additional Metadata

+/- Additional Metadata

+/- Additional Metadata

Data Entities

Data Package Usage Rights

Keywords

Methods and Protocols

People and Organizations

Temporal, Geographic and Taxonomic Coverage

Project

Maintenance

Additional Metadata

Additional Metadata

Additional Metadata