Data Package Metadata View Summary

Invasive grass litter suppresses a native grass species and promotes disease

General Information

Data Package:
Local Identifier:	edi.189.3
Title:	Invasive grass litter suppresses a native grass species and promotes disease
Alternate Identifier:	DOI PLACE HOLDER
Abstract:	Plant litter can alter ecosystems and promote plant invasions by altering resource availability, depositing phytotoxins, and transmitting microorganisms to living plants. Transmission of microorganisms from invasive plant litter to live plants may gain importance as invasive plants, which often escape pathogens upon introduction to a new range, acquire new pathogens over time. It is unclear, however, if invasive plant litter affects native plant communities by promoting disease. Microstegium vimineum is an invasive grass that suppresses native populations, in part through litter production, and has acquired new fungal leaf spot diseases since its introduction to the United States. In a greenhouse experiment, we evaluated how M. vimineum litter and its pathogens mediated competition with the native grass Elymus virginicus. Microstegium vimineum litter promoted disease on E. virginicus and suppressed establishment and biomass of both species. Litter had stronger negative effects on E. virginicus than M. vimineum, increasing the relative biomass of M. vimineum. Live plant competition reduced biomass of both species and live M. vimineum increased disease incidence on E. virginicus. Altogether, invasive grass litter suppressed both species, ultimately favoring the invasive species in competition, and increased disease incidence on the native species.
Publication Date:	2021-08-24

Time Period

Begin:

2018-05-09

End:

2018-11-04

People and Organizations
Contact:	Kendig, Amy E (University of Florida) [ email ]
Creator:	Benitez, Liliana (New College of Florida)
Creator:	Kendig, Amy E (University of Florida)
Creator:	Adhikari, Ashish (University of Florida)
Creator:	Clay, Keith (Tulane University)
Creator:	Harmon, Philip F (University of Florida)
Creator:	Holt, Robert D (University of Florida)
Creator:	Goss, Erica M (University of Florida)
Creator:	Flory, S Luke (University of Florida)

Data Entities
Data Table Name:	App. S3 height data
Description:	heights of plants in Appendix S3 experiment
Data Table Name:	App. S3 lesion data
Description:	foliar lesions on plants in Appendix S3 experiment
Data Table Name:	App. S3 leaf wetness data
Description:	leaf wetness of plants in Appendix S3 experiment
Data Table Name:	biomass data
Description:	biomass of M. vimineum and E. virginicus
Data Table Name:	establishment and lesion data
Description:	establishment and foliar lesions of M. vimineum and E. virginicus
Other Name:	App. S3 code
Description:	code for analyses and figures in Appendix S3
Other Name:	Biomass analysis
Description:	code to analyze biomass data
Other Name:	Data processing
Description:	code to process data for establishment and disease incidence analyses
Other Name:	Establishment and disease incidence analysis
Description:	code to analyze establishment and disease incidence
Other Name:	Figures and values
Description:	code for figures and values in main text
Other Name:	Residuals analysis
Description:	code to analyze model residuals
Other Name:	App. S3 experiment description
Description:	description of experiment in Appendix S3

Detailed Metadata

Data Entities

Data Table


Data:	https://pasta-s.lternet.edu/package/data/eml/edi/189/3/3ccdb9d42853e01d3b8deb5591454c0e
Name:	App. S3 height data
Description:	heights of plants in Appendix S3 experiment
Number of Records:	65
Number of Columns:	10

Table Structure

Object Name:

AppS3_heights_073018.csv

Size:

2421 bytes

Authentication:

52fe784ec628adaba07e4202093b439a Calculated By MD5

Text Format:

Number of Header Lines:

Record Delimiter:

\r\n

Orientation:

column

Simple Delimited:

Field Delimiter:	,
Quote Character:	"

Table Column Descriptions

Column Name:

Date

PotID

Species

Litter

Dry

H1_cm

H2_cm

H3_cm

H4_cm

H5_cm

Definition:

date that data were collected

ID of pot (do not indicate blocking)

species planted in pot

litter treatment applied to pot

moisture treatment applied to pot

height of plant 1 (haphazardly chosen in pot)

height of plant 2 (haphazardly chosen in pot)

height of plant 3 (haphazardly chosen in pot)

height of plant 4 (haphazardly chosen in pot)

height of plant 5 (haphazardly chosen in pot)

Storage Type:

date

float

string

float

Measurement Type:

dateTime

ratio

nominal

ratio

Measurement Values Domain:

Format	MM/DD/YY
Precision

Unit	dimensionless
Type	natural
Min	1
Max	5

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	Mv
Definition	Microstegium vimineum
Source

Code Definition

Code	Ev
Definition	Elymus virginicus
Source

Code Definition

Code	Et
Definition	Eragrostis trichodes
Source

Code Definition

Code	Pc
Definition	Dichanthelium (Panicum) clandestinum
Source

Code Definition

Code	Cc
Definition	Calamagrostis canadensis
Source

Code Definition

Code	Gs
Definition	Glyceria striata
Source

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	Y
Definition	Yes (litter was added to pot)
Source

Code Definition

Code	N
Definition	No (litter was not added to pot)
Source

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	Y
Definition	Yes (pot received dry/low moisture treatment)
Source

Code Definition

Code	N
Definition	No (pot received high moisture treatment)
Source

Unit	centimeter
Type	real
Min	2
Max	17.5

Unit	centimeter
Type	real
Min	1
Max	19.9

Unit	centimeter
Type	real
Min	1.5
Max	24.7

Unit	centimeter
Type	real
Min	0.9
Max	22

Unit	centimeter
Type	real
Min	1.1
Max	18.4

Missing Value Code:

Accuracy Report:

Accuracy Assessment:

Coverage:

Methods:

Data Table


Data:	https://pasta-s.lternet.edu/package/data/eml/edi/189/3/a1e148f854d6edbf6a310552573a9c7c
Name:	App. S3 lesion data
Description:	foliar lesions on plants in Appendix S3 experiment
Number of Records:	65
Number of Columns:	9

Table Structure

Object Name:

AppS3_Infection_091318.csv

Size:

1976 bytes

Authentication:

eb9aa5de6b9f69a4c054bbe74507c72b Calculated By MD5

Text Format:

Number of Header Lines:

Record Delimiter:

\r\n

Orientation:

column

Simple Delimited:

Field Delimiter:	,
Quote Character:	"

Table Column Descriptions

Column Name:

Date

PotID

Species

Litter

Dry

MvInf

MvNotes

NativeInf

NativeNotes

Definition:

date that data were collected

ID of pot (do not indicate blocking)

species planted in pot

litter treatment applied to pot

moisture treatment applied to pot

binary variable indicating whether at least two brown foliar lesions were observed on Microstegium vimineum plants in pot

notes concerning MvInf observations

binary variable indicating whether at least two brown foliar lesions were observed on native plants in pot

notes concerning NativeInf observations

Storage Type:

date

float

string

float

string

float

string

Measurement Type:

dateTime

ratio

nominal

ratio

nominal

ratio

nominal

Measurement Values Domain:

Format	YYYYMMDD
Precision

Unit	dimensionless
Type	natural
Min	1
Max	5

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	Mv
Definition	Microstegium vimineum
Source

Code Definition

Code	Ev
Definition	Elymus virginicus
Source

Code Definition

Code	Et
Definition	Eragrostis trichodes
Source

Code Definition

Code	Pc
Definition	Dichanthelium (Panicum) clandestinum
Source

Code Definition

Code	Cc
Definition	Calamagrostis canadensis
Source

Code Definition

Code	Gs
Definition	Glyceria striata
Source

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	Y
Definition	Yes (litter was added to pot)
Source

Code Definition

Code	N
Definition	No (litter was not added to pot)
Source

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	Y
Definition	Yes (pot received dry/low moisture treatment)
Source

Code Definition

Code	N
Definition	No (pot received high moisture treatment)
Source

Unit	dimensionless
Type	whole
Min	0
Max	1

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	black spots
Definition	black spots were observed on leaves
Source

Code Definition

Code	low
Definition	low number of brown lesions observed on leaves
Source

Code Definition

Code	high
Definition	high number of brown lesions observed on leaves
Source

Code Definition

Code	light, also black spots
Definition	light brown spots and black spots observed on leaves
Source

Code Definition

Code	low, black spots
Definition	low number of brown lesions and black spots observed on leaves
Source

Code Definition

Code	specks
Definition	lesions appeared as small specks rather than defined Bipolaris-like lesions
Source

Unit	dimensionless
Type	whole
Min	0
Max	1

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	low
Definition	low number of brown lesions observed on leaves
Source

Code Definition

Code	black spots
Definition	black spots were observed on leaves
Source

Code Definition

Code	low, black spots
Definition	low number of brown lesions and black spots observed on leaves
Source

Missing Value Code:

Code	NA
Expl	not applicable

Code	blank
Expl	no additional observations

Code	NA
Expl	not applicable

Code	blank
Expl	no additional observations

Accuracy Report:

Accuracy Assessment:

Coverage:

Methods:

Data Table


Data:	https://pasta-s.lternet.edu/package/data/eml/edi/189/3/e7f980b2e4aac63848562c1bf5744df6
Name:	App. S3 leaf wetness data
Description:	leaf wetness of plants in Appendix S3 experiment
Number of Records:	350
Number of Columns:	7

Table Structure

Object Name:

AppS3_leaf_moisture_080118.csv

Size:

13433 bytes

Authentication:

c25c42ed4b9d50e6ac38122a8e8f4534 Calculated By MD5

Text Format:

Number of Header Lines:

Record Delimiter:

\r\n

Orientation:

column

Simple Delimited:

Field Delimiter:	,
Quote Character:	"

Table Column Descriptions

Column Name:

Date

Time

PotId

Species

Treatment

LeafMoisture

Notes

Definition:

date that data were collected

time that data were collected

ID of pot (do not indicate blocking)

species planted in pot

moisture treatment applied to pot

indicator variable for whether water droplets were observed on leaf surfaces

notes about LeafMoisture observations

Storage Type:

date

float

string

Measurement Type:

dateTime

ratio

nominal

Measurement Values Domain:

Format	MM/DD/YY
Precision

Format	hh:mm
Precision

Unit	dimensionless
Type	natural
Min	1
Max	5

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	Cc
Definition	Calamagrostis canadensis
Source

Code Definition

Code	Gs
Definition	Glyceria striata
Source

Code Definition

Code	Et
Definition	Eragrostis trichodes
Source

Code Definition

Code	Ev
Definition	Elymus virginicus
Source

Code Definition

Code	Mv
Definition	Microstegium vimineum
Source

Code Definition

Code	Pc
Definition	Dichanthelium (Panicum) clandestinum
Source

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	high moisture
Definition	pot received high moisture treatment
Source

Code Definition

Code	Dry
Definition	pot received dry/low moisture treatment
Source

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	Y
Definition	Yes (water droplets were observed on leaves)
Source

Code Definition

Code	N
Definition	No (water droplets were not observed on leaves)
Source

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	Covered by litter
Definition	Water droplets could not be observed because leaves were covered by litter
Source

Code Definition

Code	But not much
Definition	Few water droples
Source

Code Definition

Code	started counting only those that have more than 2 leaves with water droplets
Definition	at 1:35 PM on the second day, only pots with at least three leaves with water droplets were assigned a Y for LeafMoisture
Source

Missing Value Code:

Code	NA
Expl	not available

Code	blank
Expl	no additional observations

Accuracy Report:

Accuracy Assessment:

Coverage:

Methods:

Data Table


Data:	https://pasta-s.lternet.edu/package/data/eml/edi/189/3/6d0a5d0cde04e35d01caad87e4b851c4
Name:	biomass data
Description:	biomass of M. vimineum and E. virginicus
Number of Records:	102
Number of Columns:	10

Table Structure

Object Name:

MvEv_Biomass_110918.csv

Size:

4761 bytes

Authentication:

54a614cce0ed763a2b4d361559dc8c78 Calculated By MD5

Text Format:

Number of Header Lines:

Record Delimiter:

\r\n

Orientation:

column

Simple Delimited:

Field Delimiter:	,
Quote Character:	"

Table Column Descriptions

Column Name:

PotID

Treatment

SpPresent

WeighedSp

Weight_g

DateWeighed

InitialsWeighed

DateSorted

InitialsSorted

Notes

Definition:

ID of pot (do not indicate blocking)

litter treatment applied to pot

species planted in pot

species biomass that was weighed

biomass weight

date that biomass was weighed

initials of person who weighed plants

date that leaves were evaluated for foliar fungal lesions

initials of person who evaluated leaves for foliar fungal lesions

notes of observations while plants were evaluated and weighed

Storage Type:

float

string

float

date

string

date

string

Measurement Type:

ratio

nominal

ratio

dateTime

nominal

dateTime

nominal

Measurement Values Domain:

Unit	dimensionless
Type	natural
Min	1
Max	6

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	None
Definition	no litter was added to the pot
Source

Code Definition

Code	Low
Definition	low litter was added to the pot (0.91 g/pot; 50 g/m2)
Source

Code Definition

Code	Med
Definition	medium litter was added to the pot (1.82 g/pot; 100 g/m2)
Source

Code Definition

Code	High
Definition	high litter was added to the pot (3.64 g/pot; 200 g/m2)
Source

Code Definition

Code	Shade
Definition	medium litter was added to the pot (1.82 g/pot; 100 g/m2) and the pot was placed under an additional shade tent. This treatment was omitted from the manuscript.
Source

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	Mv
Definition	fifty seeds of Microstegium vimineum were planted in the pot.
Source

Code Definition

Code	Ev
Definition	fifty seeds of Elymus virginicus were planted in the pot.
Source

Code Definition

Code	Ev+Mv
Definition	fifty seeds each of Microstegium vimineum and Elymus virginicus were planted in the pot.
Source

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	Mv
Definition	Weight_g corresponds to Microstegium vimineum
Source

Code Definition

Code	Ev
Definition	Weight_g corresponds to Elymus virginicus
Source

Unit	gram
Type	real
Min	0.19
Max	47.24

Format	YYYYMMDD
Precision

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	AK
Definition	Amy Kendig
Source

Format	YYYYMMDD
Precision

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	CS
Definition	Callie San Antonio
Source

Code Definition

Code	PR
Definition	Penny Reif
Source

Code Definition

Code	DN
Definition	David Notman
Source

Code Definition

Code	ZC
Definition	Sobia Chanda
Source

Code Definition

Code	AK
Definition	Amy Kendig
Source

Code Definition

Code	PR+DN
Definition	Penny Reif and David Notman
Source

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	has some mold
Definition	plants had some mold when removed from 4 degrees C for evaluating foliar lesions
Source

Code Definition

Code	intentionally not measured
Definition	biomass was not measured in this pot because it was accidentally dropped
Source

Missing Value Code:

Code	NA
Expl	not available

Code	NA
Expl	not available

Code	NA
Expl	not available

Code	NA
Expl	not available

Code	NA
Expl	not available

Code	blank
Expl	no additional observations

Accuracy Report:

Accuracy Assessment:

Coverage:

Methods:

Data Table


Data:	https://pasta-s.lternet.edu/package/data/eml/edi/189/3/7b7de07d1a58296fa8ee3a8735805644
Name:	establishment and lesion data
Description:	establishment and foliar lesions of M. vimineum and E. virginicus
Number of Records:	594
Number of Columns:	9

Table Structure

Object Name:

MvEv_GerminationInfection_PostHarvest.csv

Size:

23164 bytes

Authentication:

6e5a36e91299a222da0d08eee41b6a20 Calculated By MD5

Text Format:

Number of Header Lines:

Record Delimiter:

\r\n

Orientation:

column

Simple Delimited:

Field Delimiter:	,
Quote Character:	"

Table Column Descriptions

Column Name:

Date

PotID

Treatment

SpPresent

GermMv

GermEv

InfectedMv

InfectedEv

Notes

Definition:

date that data were collected

ID of pot (do not indicate blocking)

litter treatment applied to pot

species planted in pot

number of Microstegium vimineum plants observed in pot

number of Elymus virginicus plants observed in pot

number of Microstegium vimineum plants with at least two foliar lesions

number of Elymus virginicus plants with at least two foliar lesions

notes about observations made

Storage Type:

date

float

string

float

string

Measurement Type:

dateTime

ratio

nominal

ratio

nominal

Measurement Values Domain:

Format	YYYYMMDD
Precision

Unit	dimensionless
Type	natural
Min	1
Max	6

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	None
Definition	no litter was added to the pot
Source

Code Definition

Code	Low
Definition	low litter was added to the pot (0.91 g/pot; 50 g/m2)
Source

Code Definition

Code	Med
Definition	medium litter was added to the pot (1.82 g/pot; 100 g/m2)
Source

Code Definition

Code	High
Definition	high litter was added to the pot (3.64 g/pot; 200 g/m2)
Source

Code Definition

Code	Shade
Definition	medium litter was added to the pot (1.82 g/pot; 100 g/m2) and the pot was placed under an additional shade tent. This treatment was omitted from the manuscript.
Source

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	Mv
Definition	fifty seeds of Microstegium vimineum were planted in the pot.
Source

Code Definition

Code	Ev
Definition	fifty seeds of Elymus virginicus were planted in the pot.
Source

Code Definition

Code	Ev+Mv
Definition	fifty seeds each of Microstegium vimineum and Elymus virginicus were planted in the pot.
Source

Unit	dimensionless
Type	whole
Min	0
Max	55

Unit	dimensionless
Type	whole
Min	0
Max	132

Unit	dimensionless
Type	whole
Min	0
Max	24

Unit	dimensionless
Type	whole
Min	0
Max	31

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	not to be used for germination data
Definition	for all notes, Mv = Microstegium vimineum, Ev = Elymus virginicus, germination = establishment, infection = at least two foliar lesions
Source

Code Definition

Code	1 mv with insect damage
Definition	for all notes, Mv = Microstegium vimineum, Ev = Elymus virginicus, germination = establishment, infection = at least two foliar lesions
Source

Code Definition

Code	could be dead leaf
Definition	for all notes, Mv = Microstegium vimineum, Ev = Elymus virginicus, germination = establishment, infection = at least two foliar lesions
Source

Code Definition

Code	thinned Mv
Definition	for all notes, Mv = Microstegium vimineum, Ev = Elymus virginicus, germination = establishment, infection = at least two foliar lesions
Source

Code Definition

Code	thinned Mv 7/6
Definition	for all notes, Mv = Microstegium vimineum, Ev = Elymus virginicus, germination = establishment, infection = at least two foliar lesions
Source

Code Definition

Code	herbivory
Definition	for all notes, Mv = Microstegium vimineum, Ev = Elymus virginicus, germination = establishment, infection = at least two foliar lesions
Source

Code Definition

Code	3 could be herbivory not infection
Definition	for all notes, Mv = Microstegium vimineum, Ev = Elymus virginicus, germination = establishment, infection = at least two foliar lesions
Source

Code Definition

Code	hard to tell if infection or something else
Definition	for all notes, Mv = Microstegium vimineum, Ev = Elymus virginicus, germination = establishment, infection = at least two foliar lesions
Source

Code Definition

Code	duct tape damage to a couple of plants
Definition	for all notes, Mv = Microstegium vimineum, Ev = Elymus virginicus, germination = establishment, infection = at least two foliar lesions
Source

Code Definition

Code	other damage due to herbivory and sun? as well
Definition	for all notes, Mv = Microstegium vimineum, Ev = Elymus virginicus, germination = establishment, infection = at least two foliar lesions
Source

Code Definition

Code	mushrooms growing. One Mv may have a had 1 spot
Definition	for all notes, Mv = Microstegium vimineum, Ev = Elymus virginicus, germination = establishment, infection = at least two foliar lesions
Source

Code Definition

Code	accidentally pulled 1 Ev (included in total), 1 lesion/1 leaf/1 plant
Definition	for all notes, Mv = Microstegium vimineum, Ev = Elymus virginicus, germination = establishment, infection = at least two foliar lesions
Source

Code Definition

Code	1 lesion/1 leaf/1 plant
Definition	for all notes, Mv = Microstegium vimineum, Ev = Elymus virginicus, germination = establishment, infection = at least two foliar lesions
Source

Code Definition

Code	some Ev have lesions, but the leaves are small and crowded, so it was hard to know if it is infection and count
Definition	for all notes, Mv = Microstegium vimineum, Ev = Elymus virginicus, germination = establishment, infection = at least two foliar lesions
Source

Code Definition

Code	1-3 lesions/1 leaf/1 plant
Definition	for all notes, Mv = Microstegium vimineum, Ev = Elymus virginicus, germination = establishment, infection = at least two foliar lesions
Source

Code Definition

Code	clicker may have gotten stuck (i.e. number may be one or two higher than this)
Definition	for all notes, Mv = Microstegium vimineum, Ev = Elymus virginicus, germination = establishment, infection = at least two foliar lesions
Source

Code Definition

Code	accidentally pulled out 1 Mv and 2 Ev (included in total)
Definition	for all notes, Mv = Microstegium vimineum, Ev = Elymus virginicus, germination = establishment, infection = at least two foliar lesions
Source

Code Definition

Code	accidentally pulled out 1 Ev (included in total)
Definition	for all notes, Mv = Microstegium vimineum, Ev = Elymus virginicus, germination = establishment, infection = at least two foliar lesions
Source

Code Definition

Code	lots of herbivore scratching
Definition	for all notes, Mv = Microstegium vimineum, Ev = Elymus virginicus, germination = establishment, infection = at least two foliar lesions
Source

Code Definition

Code	1 lesion/1 leaf/1 plant, lots of herbivore scratching
Definition	for all notes, Mv = Microstegium vimineum, Ev = Elymus virginicus, germination = establishment, infection = at least two foliar lesions
Source

Code Definition

Code	accidentally pulled out 2 Evs (included in the total)
Definition	for all notes, Mv = Microstegium vimineum, Ev = Elymus virginicus, germination = establishment, infection = at least two foliar lesions
Source

Code Definition

Code	accidentally pulled 1 Ev, 1-3 lesions/1 leaf/1 plant
Definition	for all notes, Mv = Microstegium vimineum, Ev = Elymus virginicus, germination = establishment, infection = at least two foliar lesions
Source

Code Definition

Code	1-2 lesions/1-2 leaves, Ev infection on small leaves, so it is hard to know if it is infection
Definition	for all notes, Mv = Microstegium vimineum, Ev = Elymus virginicus, germination = establishment, infection = at least two foliar lesions
Source

Code Definition

Code	not to be used for germination data, clicker may have gotten stuck (i.e. number may be 1 or 2 higher)
Definition	for all notes, Mv = Microstegium vimineum, Ev = Elymus virginicus, germination = establishment, infection = at least two foliar lesions
Source

Code Definition

Code	1-3 lesions/1 leaf/ 1plant
Definition	for all notes, Mv = Microstegium vimineum, Ev = Elymus virginicus, germination = establishment, infection = at least two foliar lesions
Source

Code Definition

Code	accidentally pulled 1 Ev (included in total), 1 lesion/1 leaf/1 plant, Ev small leaves (hard to ID infection)
Definition	for all notes, Mv = Microstegium vimineum, Ev = Elymus virginicus, germination = establishment, infection = at least two foliar lesions
Source

Code Definition

Code	1-4 lesions/1-2 leaves/1 plant
Definition	for all notes, Mv = Microstegium vimineum, Ev = Elymus virginicus, germination = establishment, infection = at least two foliar lesions
Source

Code Definition

Code	accidentally pulled out 1 Ev and re-planted it (included in total)
Definition	for all notes, Mv = Microstegium vimineum, Ev = Elymus virginicus, germination = establishment, infection = at least two foliar lesions
Source

Code Definition

Code	infection may be herbivory
Definition	for all notes, Mv = Microstegium vimineum, Ev = Elymus virginicus, germination = establishment, infection = at least two foliar lesions
Source

Code Definition

Code	brown speckles on lower leaves
Definition	for all notes, Mv = Microstegium vimineum, Ev = Elymus virginicus, germination = establishment, infection = at least two foliar lesions
Source

Code Definition

Code	2-3 lesions/1-2 leaves/1 plant
Definition	for all notes, Mv = Microstegium vimineum, Ev = Elymus virginicus, germination = establishment, infection = at least two foliar lesions
Source

Code Definition

Code	1 lesion/leaf with 1-2 leaves/plant, mostly bottom leaves
Definition	for all notes, Mv = Microstegium vimineum, Ev = Elymus virginicus, germination = establishment, infection = at least two foliar lesions
Source

Code Definition

Code	Might be other infection coming from soil
Definition	for all notes, Mv = Microstegium vimineum, Ev = Elymus virginicus, germination = establishment, infection = at least two foliar lesions
Source

Code Definition

Code	Pulled out 1
Definition	for all notes, Mv = Microstegium vimineum, Ev = Elymus virginicus, germination = establishment, infection = at least two foliar lesions
Source

Code Definition

Code	Thinned
Definition	for all notes, Mv = Microstegium vimineum, Ev = Elymus virginicus, germination = establishment, infection = at least two foliar lesions
Source

Code Definition

Code	Lots of spots/senescence which could be other infection or stress- didn't count
Definition	for all notes, Mv = Microstegium vimineum, Ev = Elymus virginicus, germination = establishment, infection = at least two foliar lesions
Source

Code Definition

Code	Especially hard to count, very tangled. Pulled out 1 Mv
Definition	for all notes, Mv = Microstegium vimineum, Ev = Elymus virginicus, germination = establishment, infection = at least two foliar lesions
Source

Code Definition

Code	most of the infected were not grouped on same plants
Definition	for all notes, Mv = Microstegium vimineum, Ev = Elymus virginicus, germination = establishment, infection = at least two foliar lesions
Source

Code Definition

Code	thorough search for infection
Definition	for all notes, Mv = Microstegium vimineum, Ev = Elymus virginicus, germination = establishment, infection = at least two foliar lesions
Source

Code Definition

Code	small plants or lower leaves infected, thorough search for infection
Definition	for all notes, Mv = Microstegium vimineum, Ev = Elymus virginicus, germination = establishment, infection = at least two foliar lesions
Source

Code Definition

Code	nothing entered for Infected Mv, but there were no samples in the plastic bag
Definition	for all notes, Mv = Microstegium vimineum, Ev = Elymus virginicus, germination = establishment, infection = at least two foliar lesions
Source

Code Definition

Code	infection on edge, thorough search for infection
Definition	for all notes, Mv = Microstegium vimineum, Ev = Elymus virginicus, germination = establishment, infection = at least two foliar lesions
Source

Code Definition

Code	3 infections occurred on higher leaves with a lesion on the edge (didn't include because other searches were restricted to lower leaves), one was on a low/small plant, thorough search for infection
Definition	for all notes, Mv = Microstegium vimineum, Ev = Elymus virginicus, germination = establishment, infection = at least two foliar lesions
Source

Code Definition

Code	Mv infection on the edge (didn't record whether these were high or low leaves, so they are left in), thorough search for infection
Definition	for all notes, Mv = Microstegium vimineum, Ev = Elymus virginicus, germination = establishment, infection = at least two foliar lesions
Source

Code Definition

Code	2 infections on lower leaves, 1 is higher and on the edge (didn't include because other searches were restricted to lower leaves), thorough search for infection
Definition	for all notes, Mv = Microstegium vimineum, Ev = Elymus virginicus, germination = establishment, infection = at least two foliar lesions
Source

Code Definition

Code	Mv infection on small plant, thorough search for infection
Definition	for all notes, Mv = Microstegium vimineum, Ev = Elymus virginicus, germination = establishment, infection = at least two foliar lesions
Source

Code Definition

Code	the Ev numbers were entered in 5 High Mv, but I think they belong here because these were missing, thorough search for infection
Definition	for all notes, Mv = Microstegium vimineum, Ev = Elymus virginicus, germination = establishment, infection = at least two foliar lesions
Source

Code Definition

Code	1 infection low, 1 high and on edge (didn't include because other searches were restricted to lower leaves), thorough search for infection
Definition	for all notes, Mv = Microstegium vimineum, Ev = Elymus virginicus, germination = establishment, infection = at least two foliar lesions
Source

Missing Value Code:

Code	NA
Expl	not applicable

Code	NA
Expl	not applicable

Code	NA
Expl	not applicable

Code	NA
Expl	not applicable

Code	NA
Expl	not applicable

Code	blank
Expl	no additional observations

Accuracy Report:

Accuracy Assessment:

Coverage:

Methods:

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code for analyses and figures in Appendix S3

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Biomass analysis

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code to analyze biomass data

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code to process data for establishment and disease incidence analyses

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Establishment and disease incidence analysis

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code to analyze establishment and disease incidence

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establishment_infection_analysis.R

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Figures and values

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code for figures and values in main text

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code to analyze model residuals

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App. S3 experiment description

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description of experiment in Appendix S3

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Data Package Usage Rights

This information is released under the Creative Commons license - Attribution - CC BY (https://creativecommons.org/licenses/by/4.0/). The consumer of these data (\"Data User\" herein) is required to cite it appropriately in any publication that results from its use. The Data User should realize that these data may be actively used by others for ongoing research and that coordination may be necessary to prevent duplicate publication. The Data User is urged to contact the authors of these data if any questions about methodology or results occur. Where appropriate, the Data User is encouraged to consider collaboration or co-authorship with the authors. The Data User should realize that misinterpretation of data may occur if used out of context of the original study. While substantial efforts are made to ensure the accuracy of data and associated documentation, complete accuracy of data sets cannot be guaranteed. All data are made available \"as is.\" The Data User should be aware, however, that data are updated periodically and it is the responsibility of the Data User to check for new versions of the data. The data authors and the repository where these data were obtained shall not be liable for damages resulting from any use or misinterpretation of the data. Thank you.

Keywords

By Thesaurus:
LTER	litter, competition, invasive species, disease

Methods and Protocols

These methods, instrumentation and/or protocols apply to all data in this dataset:

Methods and protocols used in the collection of this data package

Description:

In May 2018, we collected litter from Big Oaks National Wildlife Refuge in Madison, IN, USA (38.965630, -85.364500) and transported it to the University of Florida in Gainesville, FL, USA. Microstegium vimineum has a mixed mating system of cleistogamous (obligately selfed, within leaf sheaths) and chasmogamous (potentially outcrossed, open) seeds (Baker and Dyer 2011). To minimize germination of cleistogamous seeds, we hand-picked them along with any non-M. vimineum material from the litter. We created three litter treatments within the range of litter observed at BONWR (Appendix S1): 0.91 g/pot (50 g/m2, “low”), 1.82 g/pot (100 g/m2, “medium”), 3.64 g/pot (200 g/m2, “high”), and a control treatment without litter (“none”). To enhance sporulation of pathogenic fungi in the litter, we incubated the litter for 56 hours at room temperature in one-gallon plastic bags with a paper towel saturated with deionized water. We collected M. vimineum seeds from BONWR in fall 2015 and purchased E. virginicus seeds from Prairie Moon Nursery (Winona, MN, USA) in spring 2018. On June 15, 2018, we planted three treatments: 50 seeds of E. virginicus, 50 seeds of M. vimineum (both “alone”; i.e., intraspecific competition), and 50 seeds of each (“in competition”; i.e., inter- and intraspecific competition), into 1 L (15.2 cm diameter) plastic pots filled with Metromix 930 growing medium (Sungro Horticulture, Agawam, MA, USA) saturated with tap water. We added the litter treatments to pots, creating twelve treatments (four litter treatments crossed with three planting treatments) with six replicates each. The following day, we topped each pot with a clear plastic sheet (50.8 cm width, 17.8 cm height; 0.005 Grafix Dura-Lar® film, Maple Heights, OH) formed into a cone to increase humidity and potentially enhance fungal sporulation. Cones were opened to cylinders as plants outgrew them. We placed pots under shade tents in the greenhouse to reduce heat stress (photosynthetically active radiation: 224 µMolm-2s-1 +/- 18 µMol m-2s-1), haphazardly rearranged them across two greenhouse benches weekly, and watered them daily. To measure establishment, we counted the number of plants per pot, and to measure disease incidence, we counted the proportion of plants per pot with at least two foliar lesions. We measured establishment and disease incidence weekly for six weeks. Some M. vimineum seedlings grew in E. virginicus-only pots, likely from cleistogamous seeds left in the litter despite our attempts to remove them. We counted and removed these seedlings. After 10 weeks, we cut plant stems at the soil surface and stored each species from each pot in a separate paper bag at 4°C. We then counted the number of M. vimineum plants and E. virginicus tillers (individuals were not identifiable) per pot, and the proportion with two or more foliar lesions. Because M. vimineum had few lesions, we searched for a maximum of ten minutes per M. vimineum per pot. Plants were then oven-dried at 60°C to a constant mass and weighed.

Data files and code referring to AppS3 correspond to a second experiment that is described in the PDF file AppS3_experiment.pdf. Results from this experiment are presented in Appendix S3 of the manuscript.

People and Organizations

Publishers:

Organization:

Environmental Data Initiative

Email Address:

info@environmentaldatainitiative.org

Web Address:

https://environmentaldatainitiative.org

Creators:

Individual:

Liliana Benitez

Organization:

New College of Florida

Email Address:

liliana.benitez16@ncf.edu

Id:

https://orcid.org/0000-0003-2648-6515

Individual:

Amy E Kendig

Organization:

University of Florida

Email Address:

aekendig@gmail.com

Id:

https://orcid.org/0000-0002-2774-1795

Individual:

Ashish Adhikari

Organization:

University of Florida

Email Address:

adhikariashish@ufl.edu

Id:

https://orcid.org/0000-0003-1016-0617

Individual:

Keith Clay

Organization:

Tulane University

Email Address:

clay@tulane.edu

Id:

https://orcid.org/0000-0002-3956-0887

Individual:

Philip F Harmon

Organization:

University of Florida

Email Address:

pfharmon@ufl.edu

Id:

https://orcid.org/0000-0003-2288-5148

Individual:

Robert D Holt

Organization:

University of Florida

Email Address:

rdholt@ufl.edu

Individual:

Erica M Goss

Organization:

University of Florida

Email Address:

emgoss@ufl.edu

Id:

https://orcid.org/0000-0003-3512-2107

Individual:

S Luke Flory

Organization:

University of Florida

Email Address:

flory@ufl.edu

Id:

https://orcid.org/0000-0003-3336-8613

Contacts:

Individual:

Amy E Kendig

Organization:

University of Florida

Email Address:

aekendig@gmail.com

Id:

https://orcid.org/0000-0002-2774-1795

Temporal, Geographic and Taxonomic Coverage

Temporal, Geographic and/or Taxonomic information that applies to all data in this dataset:

Time Period

Begin:

2018-05-09

End:

2018-11-04

Sampling Site:

Description:

Gainesville, FL, USA

Site Coordinates:

Longitude (degree):

-82.36

Latitude (degree):

29.64

Project

Parent Project Information:

Title:

No project title to report

Personnel:

Individual:

Keith Clay

Organization:

Tulane University

Email Address:

clay@tulane.edu

Id:

https://orcid.org/0000-0002-3956-0887

Role:

Principal Investigator

Funding:

USDA 2017-67013-26870

Related Project:

Title:

No project title to report

Personnel:

Individual:

Philip F Harmon

Organization:

University of Florida

Email Address:

pfharmon@ufl.edu

Id:

https://orcid.org/0000-0003-2288-5148

Role:

Principal Investigator

Funding:

USDA 2017-67013-26870

Related Project:

Title:

No project title to report

Personnel:

Individual:

Robert D Holt

Organization:

University of Florida

Email Address:

rdholt@ufl.edu

Role:

Principal Investigator

Funding:

USDA 2017-67013-26870

Related Project:

Title:

No project title to report

Personnel:

Individual:

Erica M Goss

Organization:

University of Florida

Email Address:

emgoss@ufl.edu

Id:

https://orcid.org/0000-0003-3512-2107

Role:

Principal Investigator

Funding:

USDA 2017-67013-26870

Related Project:

Title:

No project title to report

Personnel:

Individual:

S Luke Flory

Organization:

University of Florida

Email Address:

flory@ufl.edu

Id:

https://orcid.org/0000-0003-3336-8613

Role:

Principal Investigator

Funding:

USDA 2017-67013-26870

Maintenance

Maintenance:

Description:	completed
Frequency:

Additional Info

Additional Information:

To run analyses, create an RStudio project with the following folders: code, data, intermediate-data, output. The code (files ending in .R) and data (files ending in .csv) from this archive should be placed in their respective folders. Run 'establishment_data_processing.R' first. Either 'biomass_analysis.R' or 'establishment_infection_analysis.R' can be run next to generate statistical models for biomass, establishment, and disease incidence. Run 'residual_infection_analysis.R' to generate statistical models for relationships between disease incidence and establishment or biomass. Run 'figures_values.R' to generate figures in manuscript. Run 'appendixS3.R' to generate figures in Appendix S3 of manuscript.

More information about this dataset can be found in: Benitez, L., A. E. Kendig, A. Adhikari, K. Clay, R. D. Holt, E. Goss, S. Luke Flory. 2020. Invasive grass litter suppresses a native grass species and promotes disease. bioRxiv. https://doi.org/10.1101/2021.04.07.437244

Other Metadata

Copyright 2024 Environmental Data Initiative. This material is based upon work supported by the National Science Foundation under grants #2223103 and #2223104. Any opinions, findings, conclusions, or recommendations expressed in the material are those of the author(s) and do not necessarily reflect the views of the National Science Foundation. Please contact us with questions, comments, or for technical assistance regarding this web site or the Environmental Data Initiative. Please read our privacy policy to know what information we collect about you and to understand your privacy rights.

EDI is a collaboration between the University of New Mexico and the University of Wisconsin – Madison, Center for Limnology:

Data Package Metadata View Summary

Invasive grass litter suppresses a native grass species and promotes disease

Data Entities

Data Table

Data Table

Data Table

Data Table

Data Table

Non-Categorized Data Resource

Non-Categorized Data Resource

Non-Categorized Data Resource

Non-Categorized Data Resource

Non-Categorized Data Resource

Non-Categorized Data Resource

Non-Categorized Data Resource

Data Package Usage Rights

Keywords

Methods and Protocols

These methods, instrumentation and/or protocols apply to all data in this dataset:

People and Organizations

Temporal, Geographic and Taxonomic Coverage

Project

Parent Project Information:

Maintenance

Additional Info

Recently Added

Recently Updated

Data Package Metadata View Summary

Invasive grass litter suppresses a native grass species and promotes disease

+/- Data Entities

Data Table

Data Table

Data Table

Data Table

Data Table

Non-Categorized Data Resource

Non-Categorized Data Resource

Non-Categorized Data Resource

Non-Categorized Data Resource

Non-Categorized Data Resource

Non-Categorized Data Resource

Non-Categorized Data Resource

+/- Data Package Usage Rights

+/- Keywords

+/- Methods and Protocols

These methods, instrumentation and/or protocols apply to all data in this dataset:

+/- People and Organizations

+/- Temporal, Geographic and Taxonomic Coverage

+/- Project

Parent Project Information:

+/- Maintenance

+/- Additional Info

Data Entities

Data Package Usage Rights

Keywords

Methods and Protocols

People and Organizations

Temporal, Geographic and Taxonomic Coverage

Project

Maintenance

Additional Info