Data Package Metadata View Summary

Phospholipid Fatty Acid Profiles of Bacteria and Fungi in Peat Exposed to Experimentally Increased N Deposition, 2015

General Information

Data Package:
Local Identifier:	edi.255.5
Title:	Phospholipid Fatty Acid Profiles of Bacteria and Fungi in Peat Exposed to Experimentally Increased N Deposition, 2015
Alternate Identifier:	DOI PLACE HOLDER
Abstract:	Development of the oil sands has led to increasing atmospheric N deposition, with values as high as 17 kg N ha^-1 yr^-1; regional background levels <2 kg N ha^-1 yr^-1. Bogs, being ombrotrophic, may be especially susceptible to increasing N deposition. To examine responses to N deposition, over five years, we experimentally applied N (as NH₄NO₃) to a bog near Mariana Lakes, Alberta, at rates of 0, 5, 10, 15, 20, and 25 kg N ha^-1 yr^-1, plus controls (no water or N addition). In July of 2015 we measured PLFA markers in two depths in each plot. For the most part, microbial group abundances were not affected by increasing N input (Fig. 16). However, actinomycete abundance decreased with increasing N deposition at rates that were similar in 0-5 and 5-10 cm peat. Gram-negative bacteria increased slightly with increasing N input and were more abundant in 0-5 cm than in 5-10 cm peat; correspondingly the Gram-positive to Gram-negative bacterial ratio decreased with increasing N input and was lower in 0-5 cm than in 5-10 cm peat. Total microbial abundance and total bacterial abundance were significantly higher in 0-5 cm peat than in 5-10 cm peat. It may be that more sensitive/targeted techniques, such as high-throughput pyrosequencing, 16s RNA clone library analysis and rRNA-targeted fluorescence in situ hybridization (FISH) or whole genome shotgun sequencing may be required to reveal bog microbial community responses to N loading.
Publication Date:	2019-04-10

Time Period

Begin:

2015-07-01

End:

2015-07-30

People and Organizations
Contact:	Wieder, R Kelman (Villanova University) [ email ]
Creator:	Wieder, R Kelman (Villanova University)
Creator:	Vitt, Dale H (Southern Illinois University)
Creator:	Vile, Melanie A (Villanova University)
Creator:	Graham, Jeremy A (Southern Illinois University)
Creator:	Hartsock, Jeremy A (Southern Illinois University)
Creator:	Fillingim, Hope (Villanova University)
Creator:	House, Melissa (Southern Illinois University)
Creator:	Quinn, James C (Villanova University)
Creator:	Scott, Kimberli D (Villanova University)
Creator:	Petix, Meaghan (Southern Illinois University)
Creator:	McMillen, Kelly J (Villanova University)

Data Entities
Data Table Name:	PLFA Data
Description:	PLFA Data

Detailed Metadata

Data Entities

Data Table


Data:	https://pasta-s.lternet.edu/package/data/eml/edi/255/5/e003edac138f1178ea1f7163adbb309f
Name:	PLFA Data
Description:	PLFA Data
Number of Records:	42
Number of Columns:	11

Table Structure

Object Name:

PLFA.csv

Size:

2386 byte

Authentication:

37d602cc65173ef8be7f098016c635ab Calculated By MD5

Text Format:

Number of Header Lines:

Record Delimiter:

\r\n

Orientation:

column

Simple Delimited:

Field Delimiter:

Table Column Descriptions

Column Name:

NTRT

ARM

DEPTH

TOTAL_MICROBIAL

TOTAL_BACTERIAL

GRAM_POSITIVE_BACTERIAL

GRAM_NEGATIVE_BACTERIAL

FUNGAL

ACTINOMYCETE

FUNGAL_BACTERIAL_RATIO

GRAM_POSITIVE_GRAM_NEGATIVE_RATIO

Definition:

Field experimental N treatments were made with 8 applications per plot per year between mid May and mid August of each year

Experimental plots were located along 3 arms; each arm had one replicate each of 8 N treatments

Depth in peat from which each sample was collected in centimeters

Amount of Phospholipid Fatty Acids of all microbes measured in dry peat and expressed as micrograms of PLFA per gram of dried peat

Amount of bacterial Phospholipid Fatty Acids measured in dry peat and expressed as micrograms of PLFA per gram of dried peat

Amount of gram-positive bacterial Phospholipid Fatty Acids measured in dry peat and expressed as micrograms of PLFA per gram of dried peat

Amount of gram-negative bacterial Phospholipid Fatty Acids measured in dry peat and expressed as micrograms of PLFA per gram of dried peat

Amount of fungal Phospholipid Fatty Acids measured in dry peat and expressed as micrograms of PLFA per gram of dried peat

Amount of Actinomycete Phospholipid Fatty Acids measured in dry peat and expressed as micrograms of PLFA per gram of dried peat

The ratio of Fungal to Bacterial Phospholipid Fatty Acids in a sample

The ratio of gram-positive to gram-negative bacterial Phospholipid Fatty Acids in a sample

Storage Type:

string

float

Measurement Type:

nominal

ratio

Measurement Values Domain:

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	C
Definition	Control, no water, no N added
Source

Code Definition

Code	0
Definition	0 kg N/ha/yr added as NH4NO3
Source

Code Definition

Code	5
Definition	5 kg N/ha/yr added as NH4NO3
Source

Code Definition

Code	10
Definition	10 kg N/ha/yr added as NH4NO3
Source

Code Definition

Code	15
Definition	15 kg N/ha/yr added as NH4NO3
Source

Code Definition

Code	20
Definition	20 kg N/ha/yr added as NH4NO3
Source

Code Definition

Code	25
Definition	25 kg N/ha/yr added as NH4NO3
Source

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	1
Definition	Arm 1
Source

Code Definition

Code	2
Definition	Arm 2
Source

Code Definition

Code	3
Definition	Arm 3
Source

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	0-5
Definition	0-5 cm
Source

Code Definition

Code	5-10
Definition	5-10 cm
Source

Unit	microgramsPerGram
Type	real
Min	10.77
Max	77.62

Unit	microgramsPerGram
Type	real
Min	5.31
Max	55.69

Unit	microgramsPerGram
Type	real
Min	1.33
Max	14.34

Unit	microgramsPerGram
Type	real
Min	3.45
Max	41.44

Unit	microgramsPerGram
Type	real
Min	1.81
Max	37.1

Unit	microgramsPerGram
Type	real
Min	0.33
Max	2.74

Unit	dimensionless
Type	real
Min	0.12
Max	1.57

Unit	dimensionless
Type	real
Min	0.07
Max	0.85

Missing Value Code:

Code	-99999
Expl	Missing value

Code	-99999
Expl	Missing value

Code	-99999
Expl	Missing value

Code	-99999
Expl	Missing value

Code	-99999
Expl	Missing value

Code	-99999
Expl	Missing value

Code	-99999
Expl	Missing value

Code	-99999
Expl	Missing value

Code	-99999
Expl	Missing value

Code	-99999
Expl	Missing value

Code	-99999
Expl	Missing value

Accuracy Report:

Accuracy Assessment:

Coverage:

Methods:

Data Package Usage Rights

This data package is released to the “public domain” under Creative Commons CC0 1.0 “No Rights Reserved” (see: https://creativecommons.org/publicdomain/zero/1.0/). It is considered professional etiquette to provide attribution of the original work if this data package is shared in whole or by individual components. A generic citation is provided for this data package on the website https://portal.edirepository.org (herein “website”) in the summary metadata page. Communication (and collaboration) with the creators of this data package is recommended to prevent duplicate research or publication. This data package (and its components) is made available “as is” and with no warranty of accuracy or fitness for use. The creators of this data package and the website shall not be liable for any damages resulting from misinterpretation or misuse of the data package or its components. Periodic updates of this data package may be available from the website. Thank you.

Keywords

By Thesaurus:
(No thesaurus)	Gram-negative bacteria, gram-positive bacteria, bacteria, fungi

Methods and Protocols

These methods, instrumentation and/or protocols apply to all data in this dataset:

Methods and protocols used in the collection of this data package

Description:

In July 2015, we collected a surface peat core (10 cm diam.) from each plot and divided it into 0-5 and 5-10 cm depth increments. Samples were frozen and shipped to Ward Laboratories, Kearney, Nebraska, for quantification of phospholipid fatty acid (PLFA) profiles (cf. Andersen et al. 2010). Bacterial PLFA biomarkers (i14:0, i15:0, a15:0, 15:0, i16:0, 16:1ω7c, 16:1ω9c, i17:0, a17:0, 17:0, 17:0cy, 18:1ω7c, 19:0 cy) followed Frostegard and Bååth (1996), Högberg et al. (2007), and Stromberger et al. (2012). Gram-negative bacterial biomarkers (16:1ω7c, 16:1ω9c, 18:1ω7c, 17:0cy, 19:0cy) and gram-positive biomarkers (i14:0, i15:0, a15:0, i16:0, i17:0, a17:0) followed Stromberger et al. (2012). Actinomycete biomarkers (10Me16:0, 10Me17:0, 10Me18:0) followed Frostegård et al. (1993). Fungal biomarkers (18:2ω6c, 18:2ω9c, 18:1ω9c) followed Högberg et al. (2007) and Cardinali et al. (2015).

People and Organizations

Creators:

Individual:

R Kelman Wieder

Organization:

Villanova University

Email Address:

kelman.wieder@villanova.edu

Id:

https://orcid.org/0000-0001-5856-8473

Individual:	Dale H Vitt
Organization:	Southern Illinois University

Individual:

Melanie A Vile

Organization:

Villanova University

Email Address:

mvile@villanova.edu

Individual:

Jeremy A Graham

Organization:

Southern Illinois University

Email Address:

jeremyg@mtu.edu

Individual:

Jeremy A Hartsock

Organization:

Southern Illinois University

Email Address:

hartsock@siu.edu

Individual:

Hope Fillingim

Organization:

Villanova University

Email Address:

fillingimhope@gmail.com

Individual:

Melissa House

Organization:

Southern Illinois University

Email Address:

mhouse@siu.edu

Individual:

James C Quinn

Organization:

Villanova University

Email Address:

jcqui03@gmail.com

Individual:

Kimberli D Scott

Organization:

Villanova University

Email Address:

kscott@villanova.edu

Individual:

Meaghan Petix

Organization:

Southern Illinois University

Email Address:

meaghan.petix@wsu.edu

Individual:

Kelly J McMillen

Organization:

Villanova University

Email Address:

Kelly.Mcmillen@ttu.edu

Contacts:

Individual:

R Kelman Wieder

Organization:

Villanova University

Email Address:

kelman.wieder@villanova.edu

Id:

https://orcid.org/0000-0001-5856-8473

Temporal, Geographic and Taxonomic Coverage

Temporal, Geographic and/or Taxonomic information that applies to all data in this dataset:

Time Period

Begin:

2015-07-01

End:

2015-07-30

Sampling Site:

Description:

Alberta, Canada, 100 km south of Fort McMurray, Canada

Site Coordinates:

Longitude (degree):

-112.094

Latitude (degree):

55.895

Project

Parent Project Information:

Title:

Nitrogen Addition Experiments in Boreal Ecosystems: Understanding the Fate of Atmospherically Deposited Nitrogen in order to Determine Nitrogen Critical Loads

Personnel:

Individual:

R Wieder

Email Address:

kelman.wieder@villanova.edu

Id:

https://orcid.org/0000-0001-5856-8473

Role:

Principal Investigator

Funding:

Cumulative Environmental Management Association (CEMA): 2011-0003

Maintenance

Maintenance:

Description:	completed
Frequency:

Other Metadata

Copyright 2024 Environmental Data Initiative. This material is based upon work supported by the National Science Foundation under grants #2223103 and #2223104. Any opinions, findings, conclusions, or recommendations expressed in the material are those of the author(s) and do not necessarily reflect the views of the National Science Foundation. Please contact us with questions, comments, or for technical assistance regarding this web site or the Environmental Data Initiative. Please read our privacy policy to know what information we collect about you and to understand your privacy rights.

EDI is a collaboration between the University of New Mexico and the University of Wisconsin – Madison, Center for Limnology:

Data Package Metadata View Summary

Phospholipid Fatty Acid Profiles of Bacteria and Fungi in Peat Exposed to Experimentally Increased N Deposition, 2015

Data Entities

Data Table

Data Package Usage Rights

Keywords

Methods and Protocols

These methods, instrumentation and/or protocols apply to all data in this dataset:

People and Organizations

Temporal, Geographic and Taxonomic Coverage

Project

Parent Project Information:

Maintenance

Recently Added

Recently Updated

Data Package Metadata View Summary

Phospholipid Fatty Acid Profiles of Bacteria and Fungi in Peat Exposed to Experimentally Increased N Deposition, 2015

+/- Data Entities

Data Table

+/- Data Package Usage Rights

+/- Keywords

+/- Methods and Protocols

These methods, instrumentation and/or protocols apply to all data in this dataset:

+/- People and Organizations

+/- Temporal, Geographic and Taxonomic Coverage

+/- Project

Parent Project Information:

+/- Maintenance

Data Entities

Data Package Usage Rights

Keywords

Methods and Protocols

People and Organizations

Temporal, Geographic and Taxonomic Coverage

Project

Maintenance