Data Package Metadata View Summary

Phospholipid Fatty Acid Profiles of Bacteria and Fungi in Poor Fen Peat Exposed to Experimentally Increased N Deposition, 2015

General Information

Data Package:
Local Identifier:	edi.342.2
Title:	Phospholipid Fatty Acid Profiles of Bacteria and Fungi in Poor Fen Peat Exposed to Experimentally Increased N Deposition, 2015
Alternate Identifier:	DOI PLACE HOLDER
Abstract:	Development of the oil sands has led to increasing atmospheric N deposition, with values as high as 17 kg N ha-1 yr-1; regional background levels <2 kg N ha-1 yr-1. To examine responses to N deposition, over five years, we experimentally applied N (as NH4NO3) to a poor fen near Mariana Lake, Alberta, at rates of 0, 5, 10, 15, 20, and 25 kg N ha-1 yr-1, plus controls (no water or N addition). In July of 2015 we measured PLFA markers in two depths in each plot. Fungal abundance increased at N addition levels above 16.6 kg N ha-1 yr-1 and total bacterial abundance also increased at N addition levels above 17.1 kg N ha-1 yr-1, such that the fungal:bacterial ratio was not significantly affected by N addition. Total microbial, gram-negative bacterial, and actinomycete abundance also showed an apparent threshold responses to N addition at 16-17 kg N ha-1 yr-1 addition levels.It may be that more sensitive/targeted techniques, such as high-throughput pyrosequencing, 16s RNA clone library analysis and rRNA-targeted fluorescence in situ hybridization (FISH) or whole genome shotgun sequencing may be required to reveal detailed fen microbial community responses to N loading.
Publication Date:	2020-03-02

Time Period

Begin:

2015-07-01

End:

2015-07-31

People and Organizations
Contact:	Wieder, R Kelman (Villanova University) [ email ]
Creator:	Wieder, R Kelman (Villanova University)
Creator:	Vitt, Dale H (Southern Illinois University)
Creator:	Vile, Melanie A (Villanova University)
Creator:	Graham, Jeremy A (Southern Illinois University)
Creator:	Hartsock, Jeremy A (Southern Illinois University)
Creator:	Popma, Jacqueline M.A. (University of Michigan)
Creator:	Fillingim, Hope (Villanova University)
Creator:	House, Melissa (Southern Illinois University)
Creator:	Quinn, James C (Villanova University)
Creator:	Scott, Kimberli D (Villanova University)
Creator:	Petix, Meaghan (Southern Illinois University)
Creator:	McMillen, Kelly J (Villanova University)

Data Entities
Data Table Name:	Net N Mineralization
Description:	Net N Mineralization

Detailed Metadata

Data Entities

Data Table


Data:	https://pasta-s.lternet.edu/package/data/eml/edi/342/2/b6d42ba12535f3ecbbb312db58fcd7c1
Name:	Net N Mineralization
Description:	Net N Mineralization
Number of Records:	40
Number of Columns:	13

Table Structure

Object Name:

PLFA.csv

Size:

2470 bytes

Authentication:

e5852a682b09a6f083096db0e29129dd Calculated By MD5

Text Format:

Number of Header Lines:

Record Delimiter:

\r\n

Orientation:

column

Simple Delimited:

Field Delimiter:

Table Column Descriptions

Column Name:

ARM

NTRT

NDEP

PLOTID

DEPTH

TOTBIOM

TBACT

TGPOS

TGNEG

TFUNGI

TACTIN

FBRATIO

POSNEG

Definition:

Replicate groups of treatments were on a series of 3 Arms

Field experimental N treatments were made with 8 applications per plot per year between mid May and mid August of each year

Total N added to each of the N treatments including background N deposition (2 kg N/ha/yr in precipitation) and 1.1 kg N/ha/yr in stock water used for fertilization

Each plot of the experiment has a designated identification number

Depth in peat from which each sample was collected in centimeters

Amount of Phospholipid Fatty Acids of all microbes measured in dry peat and expressed as micrograms of PLFA per gram of dried peat

Amount of bacterial Phospholipid Fatty Acids measured in dry peat and expressed as micrograms of PLFA per gram of dried peat

Amount of gram-positive bacterial Phospholipid Fatty Acids measured in dry peat and expressed as micrograms of PLFA per gram of dried peat

Amount of gram-negative bacterial Phospholipid Fatty Acids measured in dry peat and expressed as micrograms of PLFA per gram of dried peat

Amount of fungal Phospholipid Fatty Acids measured in dry peat and expressed as micrograms of PLFA per gram of dried peat

Amount of Actinomycete Phospholipid Fatty Acids measured in dry peat and expressed as micrograms of PLFA per gram of dried peat

The ratio of Fungal to Bacterial Phospholipid Fatty Acids in a sample

The ratio of gram-positive to gram-negative bacterial Phospholipid Fatty Acids in a sample

Storage Type:

string

float

Measurement Type:

nominal

ratio

Measurement Values Domain:

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	1
Definition	Arm 1
Source

Code Definition

Code	2
Definition	Arm 2
Source

Code Definition

Code	3
Definition	Arm 3
Source

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	C
Definition	Control, no water, no N added
Source

Code Definition

Code	0
Definition	0 kg N/ha/yr added as NH4-NO3
Source

Code Definition

Code	5
Definition	5 kg N/ha/yr added as NH4-NO3
Source

Code Definition

Code	10
Definition	10 kg N/ha/yr added as NH4-NO3
Source

Code Definition

Code	15
Definition	15 kg N/ha/yr added as NH4-NO3
Source

Code Definition

Code	20
Definition	20 kg N/ha/yr added as NH4-NO3
Source

Code Definition

Code	25
Definition	25 kg N/ha/yr added as NH4-NO3
Source

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	2
Definition	2 kg N/ha/yr added as NH4-NO3
Source

Code Definition

Code	3.1
Definition	3.1 kg N/ha/yr added as NH4-NO3
Source

Code Definition

Code	8.1
Definition	8.1 kg N/ha/yr added as NH4-NO3
Source

Code Definition

Code	13.1
Definition	13.1 kg N/ha/yr added as NH4-NO3
Source

Code Definition

Code	18.1
Definition	18.1 kg N/ha/yr added as NH4-NO3
Source

Code Definition

Code	23.1
Definition	23.1 kg N/ha/yr added as NH4-NO3
Source

Code Definition

Code	28.1
Definition	28.1 kg N/ha/yr added as NH4-NO3
Source

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	1
Definition	Plot 1
Source

Code Definition

Code	2
Definition	Plot 2
Source

Code Definition

Code	3
Definition	Plot 3
Source

Code Definition

Code	4
Definition	Plot 4
Source

Code Definition

Code	5
Definition	Plot 5
Source

Code Definition

Code	6
Definition	Plot 6
Source

Code Definition

Code	7
Definition	Plot 7
Source

Code Definition

Code	8
Definition	Plot 8
Source

Code Definition

Code	9
Definition	Plot 9
Source

Code Definition

Code	10
Definition	Plot 10
Source

Code Definition

Code	12
Definition	Plot 12
Source

Code Definition

Code	13
Definition	Plot 13
Source

Code Definition

Code	14
Definition	Plot 14
Source

Code Definition

Code	15
Definition	Plot 15
Source

Code Definition

Code	16
Definition	Plot 16
Source

Code Definition

Code	17
Definition	Plot 17
Source

Code Definition

Code	19
Definition	Plot 18
Source

Code Definition

Code	20
Definition	Plot 20
Source

Code Definition

Code	21
Definition	Plot 21
Source

Code Definition

Code	11
Definition	Plot 11
Source

Code Definition

Code	18
Definition	Plot 18
Source

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	0-5
Definition	0-5 centimeters
Source

Code Definition

Code	5-10
Definition	5-10 centimeters
Source

Unit	nanogramsPerGram
Type	natural
Min	17985
Max	174086

Unit	nanogramsPerGram
Type	natural
Min	4017
Max	68590

Unit	nanogramsPerGram
Type	natural
Min	382
Max	8119

Unit	nanogramsPerGram
Type	natural
Min	1912
Max	63628

Unit	nanogramsPerGram
Type	natural
Min	831
Max	19377

Unit	nanogramsPerGram
Type	natural
Min	52
Max	1155

Unit	dimensionless
Type	real
Min	0.09
Max	1.19

Unit	dimensionless
Type	real
Min	0.03
Max	0.9

Missing Value Code:

Code	-99999
Expl	Missing Value

Code	-99999
Expl	Missing Value

Code	-99999
Expl	Missing Value

Code	-99999
Expl	Missing Value

Code	-99999
Expl	Missing Value

Code	-99999
Expl	Missing Value

Code	-99999
Expl	Missing Value

Code	-99999
Expl	Missing Value

Code	-99999
Expl	Missing Value

Code	-99999
Expl	Missing Value

Code	-99999
Expl	Missing Value

Code	-99999
Expl	Missing Value

Code	-99999
Expl	Missing Value

Accuracy Report:

Accuracy Assessment:

Coverage:

Methods:

Data Package Usage Rights

This data package is released to the "public domain" under Creative Commons CC0 1.0 "No Rights Reserved" (see: https://creativecommons.org/publicdomain/zero/1.0/). It is considered professional etiquette to provide attribution of the original work if this data package is shared in whole or by individual components. A generic citation is provided for this data package on the website https://portal.edirepository.org (herein "website") in the summary metadata page. Communication (and collaboration) with the creators of this data package is recommended to prevent duplicate research or publication. This data package (and its components) is made available "as is" and with no warranty of accuracy or fitness for use. The creators of this data package and the website shall not be liable for any damages resulting from misinterpretation or misuse of the data package or its components. Periodic updates of this data package may be available from the website. Thank you.

Keywords

By Thesaurus:
(No thesaurus)	Villanova Peatland Biogeochemistry Group, MLPF, PLFA
LTER Controlled Vocabulary	fens, peatland, nitrogen

Methods and Protocols

These methods, instrumentation and/or protocols apply to all data in this dataset:

Methods and protocols used in the collection of this data package

Description:

In July 2015, we collected a surface peat core (10 cm diam.) from each plot and divided it into 0-5 and 5-10 cm depth increments. Samples were frozen and shipped to Ward Laboratories, Kearney, Nebraska, for quantification of phospholipid fatty acid (PLFA) profiles (cf. Andersen et al. 2010). Bacterial PLFA biomarkers (i14:0, i15:0, a15:0, 15:0, i16:0, 16:1ω7c, 16:1ω9c, i17:0, a17:0, 17:0, 17:0cy, 18:1ω7c, 19:0 cy) followed Frostegård and Bååth (1996), Högberg et al. (2007), and Stromberger et al. (2012). Gram-negative bacterial biomarkers (16:1ω7c, 16:1ω9c, 18:1ω7c, 17:0cy, 19:0cy) and gram-positive biomarkers (i14:0, i15:0, a15:0, i16:0, i17:0, a17:0) followed Stromberger et al. (2012). Actinomycete biomarkers (10Me16:0, 10Me17:0, 10Me18:0) followed Frostegård et al. (1993). Fungal biomarkers (18:2ω6c, 18:2ω9c, 18:1ω9c) followed Högberg et al. (2007) and Cardinali et al. (2015).

People and Organizations

Creators:

Individual:

R Kelman Wieder

Organization:

Villanova University

Email Address:

kelman.wieder@villanova.edu

Id:

https://orcid.org/0000-0001-5856-8473

Individual:

Dale H Vitt

Organization:

Southern Illinois University

Email Address:

dvitt@siu.edu

Individual:

Melanie A Vile

Organization:

Villanova University

Email Address:

mvile@villanova.edu

Individual:

Jeremy A Graham

Organization:

Southern Illinois University

Email Address:

jeremyg@mtu.edu

Individual:

Jeremy A Hartsock

Organization:

Southern Illinois University

Email Address:

hartsock@siu.edu

Individual:

Jacqueline M.A. Popma

Organization:

University of Michigan

Email Address:

popmaj@umich.edu

Individual:

Hope Fillingim

Organization:

Villanova University

Email Address:

fillingimhope@gmail.com

Individual:

Melissa House

Organization:

Southern Illinois University

Email Address:

mhouse@siu.edu

Individual:

James C Quinn

Organization:

Villanova University

Email Address:

jcqui03@gmail.com

Individual:

Kimberli D Scott

Organization:

Villanova University

Email Address:

kscott@villanova.edu

Individual:

Meaghan Petix

Organization:

Southern Illinois University

Email Address:

meaghan.petix@wsu.edu

Individual:

Kelly J McMillen

Organization:

Villanova University

Email Address:

Kelly.Mcmillen@ttu.edu

Contacts:

Individual:

R Kelman Wieder

Organization:

Villanova University

Email Address:

kelman.wieder@villanova.edu

Id:

https://orcid.org/0000-0001-5856-8473

Temporal, Geographic and Taxonomic Coverage

Temporal, Geographic and/or Taxonomic information that applies to all data in this dataset:

Time Period

Begin:

2015-07-01

End:

2015-07-31

Sampling Site:

Description:

Alberta, Canada, 100 km south of Fort McMurray, Canada

Site Coordinates:

Longitude (degree):

-112.094

Latitude (degree):

55.897

Taxonomic Range:

Classification:

Rank Name:	kingdom
Rank Value:	Bacteria

Classification:

Rank Name:	kingdom
Rank Value:	Fungi

Project

Parent Project Information:

Title:

Nitrogen Addition Experiments in Boreal Ecosystems: Understanding the Fate of Atmospherically Deposited Nitrogen in order to Determine Nitrogen Critical Loads

Personnel:

Individual:

R Kelman Wieder

Organization:

Villanova University

Email Address:

kelman.wieder@villanova.edu

Id:

https://orcid.org/0000-0001-5856-8473

Role:

Principal Investigator

Funding:

Cumulative Environmental Management Association (CEMA): 2011-0003

Maintenance

Maintenance:

Description:	Complete
Frequency:

Other Metadata

Additional Metadata

additionalMetadata
        |___text '\n    '
        |___element 'metadata'
        |     |___text '\n      '
        |     |___element 'unitList'
        |     |     |___text '\n        '
        |     |     |___element 'unit'
        |     |     |     |  \___attribute 'id' = 'nanogramsPerGram'
        |     |     |     |  \___attribute 'multiplierToSI' = '1.00E-09'
        |     |     |     |  \___attribute 'name' = 'nanogramsPerGram'
        |     |     |     |  \___attribute 'parentSI' = 'kilogramsPerKilogram'
        |     |     |     |  \___attribute 'unitType' = 'massPerMass'
        |     |     |     |___text '\n          '
        |     |     |     |___element 'description'
        |     |     |     |     |___text 'nanogramsPerGram'
        |     |     |     |___text '\n        '
        |     |     |___text '\n      '
        |     |___text '\n    '
        |___text '\n  '

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Data Package Metadata View Summary

Phospholipid Fatty Acid Profiles of Bacteria and Fungi in Poor Fen Peat Exposed to Experimentally Increased N Deposition, 2015

Data Entities

Data Table

Data Package Usage Rights

Keywords

Methods and Protocols

These methods, instrumentation and/or protocols apply to all data in this dataset:

People and Organizations

Temporal, Geographic and Taxonomic Coverage

Project

Parent Project Information:

Maintenance

Additional Metadata

Recently Added

Recently Updated

Data Package Metadata View Summary

Phospholipid Fatty Acid Profiles of Bacteria and Fungi in Poor Fen Peat Exposed to Experimentally Increased N Deposition, 2015

+/- Data Entities

Data Table

+/- Data Package Usage Rights

+/- Keywords

+/- Methods and Protocols

These methods, instrumentation and/or protocols apply to all data in this dataset:

+/- People and Organizations

+/- Temporal, Geographic and Taxonomic Coverage

+/- Project

Parent Project Information:

+/- Maintenance

+/- Additional Metadata

Data Entities

Data Package Usage Rights

Keywords

Methods and Protocols

People and Organizations

Temporal, Geographic and Taxonomic Coverage

Project

Maintenance

Additional Metadata