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Fear and safety chemical cue data in crayfish

General Information
Data Package:
Local Identifier:edi.362.1
Title:Fear and safety chemical cue data in crayfish
Alternate Identifier:DOI PLACE HOLDER
Abstract:

In this data, you’ll find crayfish movement data (walking speeds and times spent in different sections of the test arena) as a response to differing concentrations and types of fear cues (predatory and alarm cues) as well as differing levels of safety cues. The organization of the fear cues and safety cues in the arena were orthogonal to each other which allows a user to examine movements along each of the different stimuli levels separately or in combination. The movement of the crayfish was digitized at 1 point per second and at a single location (center of the carapace) of the animal.

Publication Date:2021-07-02

Time Period
Begin:
2020-01-01
End:
2021-04-01

People and Organizations
Contact:Moore, Paul (Bowling Green State University, Professor) [  email ]
Creator:Moore, Paul (Bowling Green State University, Professor)
Associate:Tallant, Jason M. (University of Michigan, Data Manager, Data Manager)

Data Entities
Data Table Name:
Fear_safety_raw_data_public
Description:
The data were collected to analyze behavioral reactions of crayfish to different chemical stimuli representing predation events. These two stimuli were bass odor and alarm odor. In addition, a series of shelters were used in the test arena which created a safety gradient that ran orthogonal to the gradient created by chemical cues. The movement of crayfish and shelter use was analyzed.
Detailed Metadata

Data Entities

Data Table

Data:https://pasta-s.lternet.edu/package/data/eml/edi/362/1/a2c74e4856c5b8a1bbc05673e349b264
Name:Fear_safety_raw_data_public
Description:The data were collected to analyze behavioral reactions of crayfish to different chemical stimuli representing predation events. These two stimuli were bass odor and alarm odor. In addition, a series of shelters were used in the test arena which created a safety gradient that ran orthogonal to the gradient created by chemical cues. The movement of crayfish and shelter use was analyzed.
Number of Records:80
Number of Columns:19

Table Structure
Object Name:Fear_safety_raw_data_public.csv
Size:9135 byte
Authentication:a1ffa7793bd8739270b81552ed3800ef Calculated By MD5
Text Format:
Number of Header Lines:1
Record Delimiter:\r\n
Orientation:column
Simple Delimited:
Field Delimiter:,
Quote Character:"

Table Column Descriptions
 Trial_NumberPhaseOdorCOM_Fear_pixelCOM_Fear_Variance_pixelCOM_Safety_pixelCOM_Safety_Variance_pixelTime_Walking_sWalking_Speed_cm_per_sWalking_Speed_Fear_cm_per_sWalking_Speed_Safety_cm_per_sTime_fear_1_sTime_fear_2_sTime_fear_3_sTime_fear_4_sTime_safety_1_sTime_safety_2_sTime_safety_3_sTime_safety_4_s
Column Name:Trial_Number  
Phase  
Odor  
COM_Fear_pixel  
COM_Fear_Variance_pixel  
COM_Safety_pixel  
COM_Safety_Variance_pixel  
Time_Walking_s  
Walking_Speed_cm_per_s  
Walking_Speed_Fear_cm_per_s  
Walking_Speed_Safety_cm_per_s  
Time_fear_1_s  
Time_fear_2_s  
Time_fear_3_s  
Time_fear_4_s  
Time_safety_1_s  
Time_safety_2_s  
Time_safety_3_s  
Time_safety_4_s  
Definition:This is the number indicating the animal that was run. Each animal is run in a single trial, but in two fifteen minute segments. The first is exploratory and the second is with odorthe treatment consists of two conditions: Odor and exploratory. The exploratory phase is run first which is followed by the introduction of odor.The odor consists of two treatments, bass that have been fed crayfish or alarm odorCOM means the center of mass which is the average location for the animal over the whole trial. The Fear location is the average X component. Fear increases as x increasesThis is the variance for the center of mass along the x axisCOM means the center of mass which is the average location for the animal over the whole trial. The Safety location is the average y component. Safety increases as y increasesThis is the variance for the center of mass along the y axisThis is the time in seconds that the crayfish had a movement speed above 0.5 cm/s. This number is set as the lower level cut off.this is the average walking speed of the crayfish over the entire trial including non-moving time periodsthis is the average walking speed of the crayfish only in the x axis over the entire trial including non-moving time periodsthis is the average walking speed of the crayfish only in the y axis over the entire trial including non-moving time periodsThe testing arean is divided into a 16 square grid pattern that is 4 x 4. The four squares along the x axis represent increasing volumes of stimuli with 4 being the largest volume and 1 being the smallest volume. This is the fear axis and the variable here represents duration or time spent in this part of the arena (regardless of the y location) in seconds. It is a cumulative total over the whole trial.The testing arean is divided into a 16 square grid pattern that is 4 x 4. The four squares along the x axis represent increasing volumes of stimuli with 4 being the largest volume and 1 being the smallest volume. This is the fear axis and the variable here represents duration or time spent in this part of the arena (regardless of the y location) in seconds. It is a cumulative total over the whole trial.The testing arean is divided into a 16 square grid pattern that is 4 x 4. The four squares along the x axis represent increasing volumes of stimuli with 4 being the largest volume and 1 being the smallest volume. This is the fear axis and the variable here represents duration or time spent in this part of the arena (regardless of the y location) in seconds. It is a cumulative total over the whole trial.The testing arean is divided into a 16 square grid pattern that is 4 x 4. The four squares along the x axis represent increasing volumes of stimuli with 4 being the largest volume and 1 being the smallest volume. This is the fear axis and the variable here represents duration or time spent in this part of the arena (regardless of the y location) in seconds. It is a cumulative total over the whole trial.The testing arean is divided into a 16 square grid pattern that is 4 x 4. The four squares along the y axis represent increasing safety values of the shelters which is a reduced number of openings. The shelters with the highest safety values (4) have only 1 opening. The shelters with the greatest number of openings (4) have the lowest safety value (1). This is the safety axis and the variable here represents duration or time spent in this part of the arena (regardless of the x location) in seconds. It is a cumulative total over the whole trial.The testing arean is divided into a 16 square grid pattern that is 4 x 4. The four squares along the y axis represent increasing safety values of the shelters which is a reduced number of openings. The shelters with the highest safety values (4) have only 1 opening. The shelters with the greatest number of openings (4) have the lowest safety value (1). This is the safety axis and the variable here represents duration or time spent in this part of the arena (regardless of the x location) in seconds. It is a cumulative total over the whole trial.The testing arean is divided into a 16 square grid pattern that is 4 x 4. The four squares along the y axis represent increasing safety values of the shelters which is a reduced number of openings. The shelters with the highest safety values (4) have only 1 opening. The shelters with the greatest number of openings (4) have the lowest safety value (1). This is the safety axis and the variable here represents duration or time spent in this part of the arena (regardless of the x location) in seconds. It is a cumulative total over the whole trial.The testing area is divided into a 16 square grid pattern that is 4 x 4. The four squares along the y axis represent increasing safety values of the shelters which is a reduced number of openings. The shelters with the highest safety values (4) have only 1 opening. The shelters with the greatest number of openings (4) have the lowest safety value (1). This is the safety axis and the variable here represents duration or time spent in this part of the arena (regardless of the x location) in seconds. It is a cumulative total over the whole trial.
Storage Type:string  
string  
string  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
float  
Measurement Type:nominalnominalnominalratioratioratioratioratioratioratioratioratioratioratioratioratioratioratioratio
Measurement Values Domain:
DefinitionThis is the number indicating the animal that was run. Each animal is run in a single trial, but in two fifteen minute segments. The first is exploratory and the second is with odor
Allowed Values and Definitions
Enumerated Domain 
Code Definition
CodeExplore
Definitionthe control or time exploring arena before chemical added to water
Source
Code Definition
CodeOdor
DefinitionThe time when the chemical odor is added to the arena
Source
Allowed Values and Definitions
Enumerated Domain 
Code Definition
CodeBass
Definitionchemical odor derived from Bass
Source
Code Definition
CodeAlarm
Definitionchemical odor derived from macerating frozen crayfish and then filtering the slurry to produce a liquid
Source
Unitcenter of mass
Typereal
UnitVariance of center of mass
Typereal
Unitcenter of mass
Typereal
Unitvariance of center of mass
Typereal
Unitsecond
Typereal
UnitcentimeterPerSecond
Typereal
UnitcentimeterPerSecond
Typereal
UnitcentimeterPerSecond
Typereal
Unittime spent in fear area
Typeinteger
Unittime spent in fear area
Typeinteger
Unittime spent in fear area
Typeinteger
Unittime spent in fear area
Typeinteger
Unittime spent in safety area
Typeinteger
Unittime spent in safety area
Typeinteger
Unittime spent in safety area
Typeinteger
Unittime spent in safety area
Typeinteger
Missing Value Code:                                      
Accuracy Report:                                      
Accuracy Assessment:                                      
Coverage:                                      
Methods:                                      

Data Package Usage Rights

This information is released under the Creative Commons license - Attribution - CC BY (https://creativecommons.org/licenses/by/4.0/). The consumer of these data ("Data User" herein) is required to cite it appropriately in any publication that results from its use. The Data User should realize that these data may be actively used by others for ongoing research and that coordination may be necessary to prevent duplicate publication. The Data User is urged to contact the authors of these data if any questions about methodology or results occur. Where appropriate, the Data User is encouraged to consider collaboration or co-authorship with the authors. The Data User should realize that misinterpretation of data may occur if used out of context of the original study. While substantial efforts are made to ensure the accuracy of data and associated documentation, complete accuracy of data sets cannot be guaranteed. All data are made available "as is." The Data User should be aware, however, that data are updated periodically and it is the responsibility of the Data User to check for new versions of the data. The data authors and the repository where these data were obtained shall not be liable for damages resulting from any use or misinterpretation of the data. Thank you.

Keywords

By Thesaurus:
LTER Controlled Vocabularycrayfishes, predation
(No thesaurus)chemical signaling

Methods and Protocols

These methods, instrumentation and/or protocols apply to all data in this dataset:

Methods and protocols used in the collection of this data package
Description:

The testing arena was constructed with safety cues (shelters) first. During the exploratory phase, crayfish were allowed to move through the tank in absence of any chemical cues. After a 15 minute acclimation period, the crayfish was removed from the arena and different aliquots of predatory or alarm cues were added directly into the middle of each shelter. Immediately after the chemical cues were added, the crayfish was added back into the arena and allowed to move for 15 more minutes. All trials were video taped using high definition Sony camcorders. After the collection of the data, videos were digitized offline at a rate of 1 point per s using Xcitex Lite motion capture software. All videos were calibration to convert pixel coordinates to real world cm units. The subsequent x, y positions of the crayfish were used as the raw data for an r code to calculate movement parameters as well as duration spent in different parts of the arena.

Instrument(s):Sony HDR-CX405 cameras were used to record movement and were mounted on a tripod above the tank

People and Organizations

Publishers:
Organization:Environmental Data Initiative
Email Address:
info@environmentaldatainitiative.org
Web Address:
https://environmentaldatainitiative.org
Creators:
Individual:Dr. Paul Moore
Organization:Bowling Green State University
Position:Professor
Email Address:
pmoore@bgsu.edu
Web Address:
https://www.bgsu.edu/arts-and-sciences/neuroscience/nmb-people/faculty/paul-moore.html
Id:https://orcid.org/0000-0002-4555-1178
Contacts:
Individual:Dr Paul Moore
Organization:Bowling Green State University
Position:Professor
Email Address:
pmoorebgsu@gmail.com
Id:https://orcid.org/0000-0002-4555-1178
Associated Parties:
Individual: Jason M. Tallant
Organization:University of Michigan
Position:Data Manager
Email Address:
jtallant@umich.edu
Id:https://orcid.org/0000-0003-4790-8772
Role:Data Manager
Metadata Providers:
Individual:Dr. Paul Moore
Organization:Bowling Green State University
Position:Professor
Email Address:
pmoorebgsu@gmail.com
Id:https://orcid.org/0000-0002-4555-1178

Temporal, Geographic and Taxonomic Coverage

Temporal, Geographic and/or Taxonomic information that applies to all data in this dataset:

Time Period
Begin:
2020-01-01
End:
2021-04-01

Project

Other Metadata

Additional Metadata

additionalMetadata
        |___text '\n    '
        |___element 'metadata'
        |     |___text '\n      '
        |     |___element 'unitList'
        |     |     |___text '\n        '
        |     |     |___element 'unit'
        |     |     |     |  \___attribute 'id' = 'center of mass'
        |     |     |     |  \___attribute 'name' = 'center of mass'
        |     |     |     |___text '\n          '
        |     |     |     |___element 'description'
        |     |     |     |___text '\n        '
        |     |     |___text '\n        '
        |     |     |___element 'unit'
        |     |     |     |  \___attribute 'id' = 'time spent in safety area'
        |     |     |     |  \___attribute 'name' = 'time spent in safety area'
        |     |     |     |___text '\n          '
        |     |     |     |___element 'description'
        |     |     |     |___text '\n        '
        |     |     |___text '\n        '
        |     |     |___element 'unit'
        |     |     |     |  \___attribute 'id' = 'time spent in fear area'
        |     |     |     |  \___attribute 'name' = 'time spent in fear area'
        |     |     |     |___text '\n          '
        |     |     |     |___element 'description'
        |     |     |     |___text '\n        '
        |     |     |___text '\n        '
        |     |     |___element 'unit'
        |     |     |     |  \___attribute 'id' = 'Variance of center of mass'
        |     |     |     |  \___attribute 'name' = 'Variance of center of mass'
        |     |     |     |___text '\n          '
        |     |     |     |___element 'description'
        |     |     |     |___text '\n        '
        |     |     |___text '\n        '
        |     |     |___element 'unit'
        |     |     |     |  \___attribute 'id' = 'variance of center of mass'
        |     |     |     |  \___attribute 'name' = 'variance of center of mass'
        |     |     |     |___text '\n          '
        |     |     |     |___element 'description'
        |     |     |     |___text '\n        '
        |     |     |___text '\n      '
        |     |___text '\n    '
        |___text '\n  '

Additional Metadata

additionalMetadata
        |___text '\n    '
        |___element 'metadata'
        |     |___text '\n      '
        |     |___element 'emlEditor'
        |     |        \___attribute 'app' = 'ezEML'
        |     |        \___attribute 'release' = '2021.06.16'
        |     |___text '\n    '
        |___text '\n  '

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