Data Package Metadata View Summary

Fate and Toxicity of Engineered Nanomaterials in the Environment: a Meta-analysis

General Information

Data Package:
Local Identifier:	edi.363.1
Title:	Fate and Toxicity of Engineered Nanomaterials in the Environment: a Meta-analysis
Alternate Identifier:	DOI PLACE HOLDER
Abstract:	Engineered nanomaterials (ENMs, particles less than 100 nanometers) are being manufactured at increasing levels for a variety of reasons including cosmetics, food packaging and preservation, fertilizers, and medical technology. Thousands of metric tons of ENMs are released to soils, water bodies, air, and landfills each year. These particles have distinct properties owing to their small size and relatively large surface area to volume ratio. These characteristics can result in these materials having higher reactivity and toxicity in biological systems - especially because ENMs are small enough to enter cells. However, studies on environmental and ecological effects of ENMs have shown mixed results. The goal of this project is to address three primary research questions using meta-analysis of existing literature: 1) Which ENMs have been studied and in what context? 2) How do particle identity, size, concentration, and study duration influence toxicity (as estimated by response ratios, lethal concentration (LC50), and effective concentration (EC50)) in different organisms related to the role they play in the environment? 3) What are bioaccumulation, biomagnification, and bioconcentration factors of ENMs as a function of ecosystem role and trophic level in different organisms? We collected data from 191 published scientific papers and extracted 2102 unique observations that are used to address the questions outlined above. We calculated response ratios related to biological responses of biomass, diversity, growth, metabolism, and survival to evaluate how these are influenced by ENM exposure. Values of LC50 (concentration at which 50% of test organisms died) and EC50 (concentration at which 50% of test organisms showed an effect) were collected to estimate concentrations at which toxicity occurs. Bioaccumulation (BAF), bioconcentration (BCF), and biomagnification (BMF) factors were recorded to estimate environmental accumulation and trophic transfer. This work is a result of a graduate-level course focused on environmental problems that was offered by Dr. Walter Dodds in the Division of Biology at Kansas State University during August 2019 - December 2019.
Publication Date:	2021-07-08

Time Period

Begin:

2019-09-01

End:

2019-12-20

People and Organizations
Contact:	Guinnip, James P (Kansas State University) [ email ]
Creator:	Guinnip, James P (Kansas State University)
Creator:	Dodds, Walter K (Kansas State University)
Creator:	Schechner, Anne E (Kansas State University)
Creator:	Pfaff, Peter J (Kansas State University)
Creator:	Smith, Dylan J (Kansas State University)
Creator:	Dea, Hannah I (Kansas State University)
Creator:	Hedden, Crosby K (Kansas State University)
Creator:	Keen, Rachel M (Kansas State University)
Creator:	Wiens, Benjamin J (Kansas State University)

Data Entities
Data Table Name:	ENMsInTheEnvt
Description:	Meta-analysis data regarding environmental and ecological effects of engineered nanomaterials. Literature search conducted on 01 Nov 2019 in the Web of Science Core Collection.

Detailed Metadata

Data Entities

Data Table


Data:	https://pasta-s.lternet.edu/package/data/eml/edi/363/1/a2ffbaa042eaf7826a97ac8e9d04df23
Name:	ENMsInTheEnvt
Description:	Meta-analysis data regarding environmental and ecological effects of engineered nanomaterials. Literature search conducted on 01 Nov 2019 in the Web of Science Core Collection.
Number of Records:	2102
Number of Columns:	73

Table Structure

Object Name:

alldata formatted for EDI.csv

Size:

1469959 byte

Authentication:

51d21261011d8932f2407fcbe0428955 Calculated By MD5

Text Format:

Number of Header Lines:

Record Delimiter:

\r\n

Orientation:

column

Simple Delimited:

Field Delimiter:	,
Quote Character:	"

Table Column Descriptions

author

year

title

doi

relevance

NMspecific

coating

coatingtype

NMsize

NMsizeunits

NMconc

NMconcunits

applicationsite

presspulse

kingdom

phylum

class

order

family

genus

species

generalorganism

organism

trophiclevel

ecosystemgeneral

ecosystemspecific

settinggeneral

settingspecific

duration_days

responsegeneral

responsespecific

responseunits

expn

expmean

expsd

conn

conmean

consd

RRsignif_yn

RRp_modifier

RRp_value

response_nonRR

responseunits_nonRR

responsesd_nonRR

n_nonRR

MPcomparison_yn

MPsignif_yn

MPeffectdirection

MPp_modifier

MPp_value

IONcomparison_yn

IONsignif_yn

IONeffectdirection

IONp_modifier

IONp_value

expgeary

congeary

responseratio

RRvar

RRadj

RRadjvar

newcat

newtrophic

NMconcfixed

response_nonRRfixed

NMconcfixedunits

response_nonRRfixedunits

NMconcfixedmmol

response_nonRRfixedmmol

NMconcfixedmmolunits

response_nonRRfixedmmolunits

Column Name:

author

year

title

doi

relevance

NMspecific

coating

coatingtype

NMsize

NMsizeunits

NMconc

NMconcunits

applicationsite

presspulse

kingdom

phylum

class

order

family

genus

species

generalorganism

organism

trophiclevel

ecosystemgeneral

ecosystemspecific

settinggeneral

settingspecific

duration_days

responsegeneral

responsespecific

responseunits

expn

expmean

expsd

conn

conmean

consd

RRsignif_yn

RRp_modifier

RRp_value

response_nonRR

responseunits_nonRR

responsesd_nonRR

n_nonRR

MPcomparison_yn

MPsignif_yn

MPeffectdirection

MPp_modifier

MPp_value

IONcomparison_yn

IONsignif_yn

IONeffectdirection

IONp_modifier

IONp_value

expgeary

congeary

responseratio

RRvar

RRadj

RRadjvar

newcat

newtrophic

NMconcfixed

response_nonRRfixed

NMconcfixedunits

response_nonRRfixedunits

NMconcfixedmmol

response_nonRRfixedmmol

NMconcfixedmmolunits

response_nonRRfixedmmolunits

Definition:

First author of paper only (last name)

Year of publication

Full title of the paper

Link for accessing original papers

Group of search terms the paper was returned under in Web of Science

Relevance number assigned when all papers are downloaded from Web of Science

general target element tested in the study

specific type of nanomaterial tested in the study

yes or no entry for whether or not the nanomaterials used had a coating

specific type of coating used for nanomaterials

size of nanomaterial used in the study

units for the NMsize column

concentration of nanomaterials used in the study

concentration units for NMconc column

method of nanomaterial application

nanomaterials applied once (pulse) or maintained at a certain concentration (press)

taxonomic information

general group of organism

scientific name or specific organism information

trophic level of study organism

general ecosystem type of study organism

specific ecosystem type of study organism

general setting of experiment

specific setting of experiment

length of experiment in days

general response type in study

specific response type in study

units for expn, expmean, conn, and conmean

sample size of experiment group in the study

mean for the experiment group in the study

standard deviation for the experiment group in the study

sample size of control group in the study

mean for the control group in the study

standard deviation for the control group in the study

yes or no for whether the authors found the experimental response statistically significant or not

modifier for whether the p value from the authors' statistical test is greater than, less than, or equal to RRp_value

Actual p-value from statistical test if it's provided or significance threshold used by the authors

response value for LC50, EC50, BAF, BCF, or BMF

units for response_nonRR

standard deviation associated with response_nonRR

sample size associated with response_nonRR

yes or no for whether the authors conducted trials with a macroscale form of the element or not

yes or no for whether the test on the macroscale form of the element was significantly different than nanomaterial or not

was the effect of the nanomaterial greater, smaller, or equivalent relative to the macroscale form

modifier for whether the p value from the authors' statistical test is greater than, less than, or equal to MPp_value

Actual p-value (from statistical test with macroscale form) if it's provided or significance threshold used by the authors

yes or no for whether the authors conducted trials with an ionic form of the element or not

yes or no for whether the test on the ionic form of the element was significantly different than nanomaterial or not

was the effect of the nanomaterial greater, smaller, or equivalent relative to the ionic form

modifier for whether the p value from the authors' statistical test is greater than, less than, or equal to IONp_value

Actual p-value (from statistical test with ionic form) if it's provided or significance threshold used by the authors

Geary’s test index with small-sample correction for expmean (Eq. 13, Lajeunesse 2015, Ecology)

Geary’s test index with small-sample correction for conmean (Eq. 13, Lajeunesse 2015, Ecology)

Log response ratio (as defined by Hedges et al., 1999, Ecology)

Variance of the log response ratio (Eq. 1, Hedges et al., 1999, Ecology)

Log response ratio adjusted for small-sample bias (Eq. 8, Lajeunesse 2015, Ecology)

Variance of the log response ratio adjusted for small-sample bias (Eq. 9, Lajeunesse 2015, Ecology)

further generalization of responsegeneral

further generalization of trophiclevel

values from NMconc converted to mg/L or mg/kg

values from response_nonRR converted to mg/L or mg/kg (note: this does not apply to BAF, BMF, or BCF values)

units for NMconcfixed

units for response_nonRRfixed (note: this does not apply to BAF, BMF, or BCF values)

values from NMconcfixed column converted from mg to mmol

values from response_nonRRfixed converted from mg to mmol (note: this does not apply to BAF, BMF, or BCF values)

units for NMconcfixedmmol

units for response_nonRRfixedmmol (note: does not apply to BAF, BMF, or BCF)

Storage Type:

string

float

string

float

string

float

string

float

string

float

string

float

string

float

string

float

string

float

string

float

string

float

string

float

string

Measurement Type:

nominal

ratio

nominal

ratio

nominal

ratio

nominal

ratio

nominal

ratio

nominal

ratio

nominal

ratio

nominal

ratio

nominal

ratio

nominal

ratio

nominal

ratio

nominal

ratio

nominal

Measurement Values Domain:

Definition

First author of paper only (last name)

Unit	year
Type	integer

Definition

Full title of the paper

Definition

Link for accessing original papers

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	Aq. Producers
Definition	returned from Aquatic Producers set of search terms
Source

Code Definition

Code	Consumers
Definition	returned from Consumers set of search terms
Source

Code Definition

Code	detritivore/microbe
Definition	returned from Microbes set of search terms
Source

Code Definition

Code	extra calculations
Definition	refers to additional metrics calculated for trophic transfer factors (see Method Step "Rules for data inclusion")
Source

Code Definition

Code	Terr. Producers
Definition	returned from Terrestrial Producers set of search terms
Source

Code Definition

Code	Trophic Transfer
Definition	returned from Bioaccumulation/Biomagnification set of search terms
Source

Unit	unitless
Type	integer

Definition

general target element tested in the study

Definition

specific type of nanomaterial tested in the study

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	no
Definition	nanomaterials did not have a coating
Source

Code Definition

Code	yes
Definition	nanomaterials had a coating
Source

Definition

specific type of coating used for nanomaterials

Unit	nanometer
Type	real

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	nm
Definition	nanometers
Source

Unit	variable
Type	real

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	nan
Definition	not applicable
Source

Code Definition

Code	mg/g
Definition	milligrams per gram
Source

Code Definition

Code	mg/kg
Definition	milligrams per kilogram
Source

Code Definition

Code	mg/Kg
Definition	milligrams per kilogram
Source

Code Definition

Code	mg/L
Definition	milligrams per liter
Source

Code Definition

Code	mg/mL
Definition	milligrams per milliliter
Source

Code Definition

Code	ug/g
Definition	micrograms per gram
Source

Code Definition

Code	ug/L
Definition	micrograms per liter
Source

Code Definition

Code	ug/mL
Definition	micrograms per milliliter
Source

Code Definition

Code	ul/L
Definition	microliters per liter
Source

Code Definition

Code	uM
Definition	micromolar
Source

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	both
Definition	nanomaterials applied in the environment and through diet
Source

Code Definition

Code	diet
Definition	nanomaterials applied through diet
Source

Code Definition

Code	environment
Definition	nanomaterials applied in the environment
Source

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	press
Definition	nanomaterials maintained at a certain concentration
Source

Code Definition

Code	pulse
Definition	nanomaterials applied once
Source

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	nan
Definition	unclear or unstable taxonomy
Source

Code Definition

Code	Animalia
Definition	organism from Kingdom Animalia
Source

Code Definition

Code	Bacteria
Definition	organism from Kingdom Bacteria
Source

Code Definition

Code	Fungi
Definition	organism from Kingdom Fungi
Source

Code Definition

Code	Plantae
Definition	organism from Kingdom Plantae
Source

Definition

taxonomic information

Definition

taxonomic information

Definition

taxonomic information

Definition

taxonomic information

Definition

taxonomic information

Definition

taxonomic information

Definition

general group of organism

Definition

scientific name or specific organism information

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	Detritivore
Definition	organism feeds on dead organic matter
Source

Code Definition

Code	Microbe
Definition	microorganism
Source

Code Definition

Code	Omnivore
Definition	organism feeds on plant and animal material
Source

Code Definition

Code	Primary consumer
Definition	organism feeds on plant material
Source

Code Definition

Code	Producer
Definition	organism produces its own energy
Source

Code Definition

Code	Secondary or higher consumer
Definition	organsim feeds mainly on other consumers
Source

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	Aquatic
Definition	organism from an aquatic environment
Source

Code Definition

Code	Terrestrial
Definition	organism from a terrestrial environment
Source

Definition

specific ecosystem type of study organism

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	Field/Natural
Definition	experiment conducted in a natural ecosystem in the field
Source

Code Definition

Code	Lab
Definition	experiment conducted in a laboratory
Source

Code Definition

Code	Mesocosm
Definition	experiment conducted in a mesocosm
Source

Code Definition

Code	Microcosm
Definition	experiment conducted in a microcosm
Source

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	Greenhouse
Definition	experiment conducted in a greenhouse
Source

Code Definition

Code	Indoor
Definition	experiment conducted indoors
Source

Code Definition

Code	Outdoor
Definition	experiment conducted outdoors
Source

Unit	days
Type	real

Definition

general response type in study

Definition

specific response type in study

Definition

units for expn, expmean, conn, and conmean

Unit	unitless
Type	real

Unit	variable
Type	real

Unit	variable
Type	real

Unit	unitless
Type	real

Unit	variable
Type	real

Unit	variable
Type	real

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	nan
Definition	not applicable
Source

Code Definition

Code	no
Definition	not statistically significant
Source

Code Definition

Code	yes
Definition	statistically significant
Source

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	nan
Definition	not applicable
Source

Code Definition

Code	<
Definition	less than
Source

Code Definition

Code	=
Definition	equal to
Source

Code Definition

Code	>
Definition	greater than
Source

Unit	unitless
Type	real

Unit	variable
Type	real

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	nan
Definition	not applicable
Source

Code Definition

Code	mg Ag/kg dry soil
Definition	milligrams silver per kilogram of dry soil
Source

Code Definition

Code	mg/kg
Definition	milligrams per kilogram
Source

Code Definition

Code	mg/L
Definition	milligrams per liter
Source

Code Definition

Code	mg/l
Definition	milligrams per liter
Source

Code Definition

Code	mM
Definition	millimolar
Source

Code Definition

Code	ug/L
Definition	micrograms per liter
Source

Code Definition

Code	ug/mL
Definition	micrograms per milliliter
Source

Code Definition

Code	uM
Definition	micromolar
Source

Unit	variable
Type	real

Unit	unitless
Type	real

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	nan
Definition	not applicable
Source

Code Definition

Code	no
Definition	no comparison with macroscale form
Source

Code Definition

Code	yes
Definition	comparison with macroscale form
Source

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	nan
Definition	not applicable
Source

Code Definition

Code	no
Definition	not statistically significant
Source

Code Definition

Code	not clear
Definition	statistical significance unclear
Source

Code Definition

Code	yes
Definition	statistically significant
Source

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	nan
Definition	not applicable
Source

Code Definition

Code	greater
Definition	greater than
Source

Code Definition

Code	neither
Definition	equal to
Source

Code Definition

Code	smaller
Definition	less than
Source

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	nan
Definition	not applicable
Source

Code Definition

Code	<
Definition	less than
Source

Code Definition

Code	>
Definition	greater than
Source

Unit	unitless
Type	real

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	nan
Definition	not applicable
Source

Code Definition

Code	no
Definition	no comparison with ionic form
Source

Code Definition

Code	yes
Definition	comparison with ionic form
Source

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	nan
Definition	not applicable
Source

Code Definition

Code	no
Definition	not statistically significant
Source

Code Definition

Code	not clear
Definition	statistical significance unclear
Source

Code Definition

Code	yes
Definition	statistically significant
Source

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	nan
Definition	not applicable
Source

Code Definition

Code	greater
Definition	greater than
Source

Code Definition

Code	neither
Definition	equal to
Source

Code Definition

Code	smaller
Definition	less than
Source

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	nan
Definition	not applicable
Source

Code Definition

Code	<
Definition	less than
Source

Code Definition

Code	=
Definition	equal to
Source

Code Definition

Code	>
Definition	greater than
Source

Unit	unitless
Type	real

Unit	unitless
Type	real

Unit	unitless
Type	real

Unit	unitless
Type	real

Unit	unitless
Type	real

Unit	unitless
Type	real

Unit	unitless
Type	real

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	BAF
Definition	bioaccumulation factor
Source

Code Definition

Code	BCF
Definition	bioconcentration factor
Source

Code Definition

Code	Biomass
Definition	tissue biomass
Source

Code Definition

Code	BMF
Definition	biomagnification factor
Source

Code Definition

Code	Concentration
Definition	tissue concentration of nanomaterials originating from the environment or food
Source

Code Definition

Code	Diversity
Definition	indices of biological diversity
Source

Code Definition

Code	EC50
Definition	concentration at which 50% of the study organisms showed some type of response
Source

Code Definition

Code	Growth
Definition	growth rates of organisms
Source

Code Definition

Code	LC50
Definition	concentration at which 50% of the study organisms showed a lethal response
Source

Code Definition

Code	Metabolism
Definition	metabolic rates of organisms
Source

Code Definition

Code	NOEC
Definition	concentration below which an effect is unlikely to be observed
Source

Code Definition

Code	Survival
Definition	percent survival of a group of study organisms
Source

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	Animal
Definition	study involves primary consumer, secondary or high consumer, omnivore, or detritivore
Source

Code Definition

Code	Aquatic producer
Definition	study involves algae or aquatic macrophyte
Source

Code Definition

Code	Heterotrophic microbe
Definition	study involves heterotrophic microbes
Source

Code Definition

Code	Terrestrial producer
Definition	study involves land plants or other terrestrial producers
Source

Unit	variable
Type	real

Unit	variable
Type	real

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	nan
Definition	not applicable
Source

Code Definition

Code	mg/kg
Definition	milligrams per kilogram
Source

Code Definition

Code	mg/L
Definition	milligrams per liter
Source

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	nan
Definition	not applicable
Source

Code Definition

Code	mg/kg
Definition	milligrams per kilogram
Source

Code Definition

Code	mg/L
Definition	milligrams per liter
Source

Unit	variable
Type	real

Unit	variable
Type	real

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	nan
Definition	not applicable
Source

Code Definition

Code	mmol/kg
Definition	millimoles per kilogram
Source

Code Definition

Code	mmol/L
Definition	millimoles per liter
Source

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	nan
Definition	not applicable
Source

Code Definition

Code	mmol/kg
Definition	millimoles per kilogram
Source

Code Definition

Code	mmol/L
Definition	millimoles per liter
Source

Missing Value Code:

Code	NA
Expl	data missing

Code	99
Expl	data missing

Code	NA
Expl	data missing

Code	NA
Expl	data missing

Code	NA
Expl	data missing

Code	NA
Expl	data missing

Code	NA
Expl	data missing

Code	NA
Expl	data missing

Code	NA
Expl	data missing

Code	NA
Expl	data missing

Code	NA
Expl	data missing

Code	NA
Expl	data missing

Code	NA
Expl	data missing

Code	NA
Expl	data missing

Code	NA
Expl	data missing

Code	NA
Expl	data missing

Code	NA
Expl	data missing

Code	NA
Expl	data missing

Code	NA
Expl	data missing

Code	NA
Expl	data missing

Code	NA
Expl	data missing

Code	NA
Expl	data missing

Code	NA
Expl	data missing

Code	NA
Expl	data missing

Code	NA
Expl	data missing

Code	NA
Expl	data missing

Code	NA
Expl	data missing

Code	NA
Expl	data missing

Code	NA
Expl	data missing

Code	NA
Expl	data missing

Code	NA
Expl	data missing

Code	NA
Expl	data missing

Code	NA
Expl	data missing

Code	NA
Expl	data missing

Code	NA
Expl	data missing

Code	NA
Expl	data missing

Code	NA
Expl	data missing

Code	NA
Expl	data missing

Code	NA
Expl	data missing

Code	NA
Expl	data missing

Code	NA
Expl	data missing

Code	NA
Expl	data missing

Code	NA
Expl	data missing

Code	NA
Expl	data missing

Code	NA
Expl	data missing

Code	NA
Expl	data missing

Code	NA
Expl	data missing

Code	NA
Expl	data missing

Code	NA
Expl	data missing

Code	NA
Expl	data missing

Code	NA
Expl	data missing

Accuracy Report:

Accuracy Assessment:

Coverage:

Methods:

Data Package Usage Rights

This information is released under the Creative Commons license - Attribution - CC BY (https://creativecommons.org/licenses/by/4.0/). The consumer of these data ("Data User" herein) is required to cite it appropriately in any publication that results from its use. The Data User should realize that these data may be actively used by others for ongoing research and that coordination may be necessary to prevent duplicate publication. The Data User is urged to contact the authors of these data if any questions about methodology or results occur. Where appropriate, the Data User is encouraged to consider collaboration or co-authorship with the authors. The Data User should realize that misinterpretation of data may occur if used out of context of the original study. While substantial efforts are made to ensure the accuracy of data and associated documentation, complete accuracy of data sets cannot be guaranteed. All data are made available "as is." The Data User should be aware, however, that data are updated periodically and it is the responsibility of the Data User to check for new versions of the data. The data authors and the repository where these data were obtained shall not be liable for damages resulting from any use or misinterpretation of the data. Thank you.

Keywords

By Thesaurus:
(No thesaurus)	nanomaterials, response ratio, lethal dose, effective dose, biomagnification, bioaccumulation

Methods and Protocols

These methods, instrumentation and/or protocols apply to all data in this dataset:

Methods and protocols used in the collection of this data package

Description:

General workflow:

Our general workflow consisted of multiple steps of identifying studies, assessing their relevance to our aims, and harvesting the data. We mined the peer-reviewed literature on 1 Nov 2019 using the Web of Science Core Collection Database and extracted papers by search relevance (details below and in other method steps). We searched with terms including “nanomat*” or “nanopart*” (*indicating a search wildcard) in combination with qualifiers such as “terrestrial” or “aquatic”, and we further narrowed our search to focus on microbes, aquatic or terrestrial producers, or consumers, and specific response terms (e.g., photosynthesis, growth). Each line for response ratios had at least one comparison of experimental treatment with control, and we collected ancillary information relevant to the study for all recorded experimental results. Method Step "Detailed search terms" has detailed search terms and Method Step "Rules for data inclusion" has a list of rules for recording data and exclusion of papers.

We collected variables to be used for response ratios, EC50, ED50, LC50, LD50 (50% concentration or dose for an effect or lethality), biomagnification factors (BMF, concentration in predator versus prey, also referred to as trophic transfer factor), bioaccumulation factors (BAF, tissue concentration/environment concentration), bioconcentration factors (BCF, same as BAF but excludes dietary exposure), ENM type, size, concentration, and other experimental details. Data were reported such that negative response ratios represented harm (e.g., mortality rates were converted to survival so a decrease in survival would translate to a negative response ratio), and units were converted when needed to facilitate direct comparison.

We created a detailed list of rules to exclude non-relevant results (see Method Step "Detailed search terms"). The top 80 papers for each grouping of search terms (sorted by Web of Science relevance scores) were assigned without bias to each of nine reviewers for data extraction. We identified an additional selection of 44 papers from the remaining initial search lists using targeted if-then statements to highlight papers in areas in which we were lacking data in an effort to identify papers most likely to increase sample sizes enough to ensure rigorous analyses. Thus, we examined 444 papers closely. We rejected many of these immediately because they were reviews, or while they discussed ENMs, they did not have actual experiments on ENMs.

We recorded experimental data directly from the paper when authors made results available in a table or through in-text discussion of results. When data were only available in the form of a graph, we extracted numeric data from images using WebPlotDigitizer (Rohatgi, 2019).

Description:

Detailed search terms:

This a list of search terms we used in Web of Science to gather peer-reviewed literature for the meta-analysis, and the total number of results for each search on 01 Nov 2019:

Microbes - 1621 results: (nanomat* OR nanopart*) AND (soil* OR terrestrial* OR aquatic* OR freshwater* OR marine* OR ocean* OR saltwater* OR estuar*) AND (microb* OR bacter* OR fung* OR microorg*) AND (*toxic* OR lc50 OR ld50 OR ec50 OR ed50 OR *divers* OR biomass OR abund* OR growth OR reprod* OR bioaccum* OR biomagnif* OR photosynth* OR respir* OR metabol*)

Aquatic producers - 922 results: (nanopart* OR nanomat* )AND(aquatic* OR freshwater* OR marine* OR ocean* OR saltwater* OR estuar*) AND ( plant OR vegetat* OR macrophyte OR alga*) AND (*toxic* OR lc50 OR ld50 OR ec50 OR ed50 OR *divers* OR biomass OR abund* OR growth OR reprod* OR bioaccum* OR biomag* OR photosynth* OR respir* OR metabol* OR germinat* OR phenology germinat* OR phenolog*)

Terrestrial producers - 991 results: (nanomat* OR nanopart*) AND (plant* OR vegetat* OR crop*) AND (terrestr* OR soil*) AND (*toxic* OR EC50 OR ED50 OR LC50 OR LD50 OR *divers* OR biomass OR abund* OR growth OR photosynth* OR respir* OR metabol* OR germinat* OR phenolog* OR bioaccum*)

Consumers - 1024 results: (nanomat* OR nanopart*) AND (soil* OR terrestrial* OR aquatic* OR freshwater* OR marine* OR ocean* OR saltwater* OR estuar*) AND (animal* OR vertebrat* OR fish* OR consume*) AND (*toxic* OR LC50 OR LD50 OR EC50 OR ED50 OR *divers* OR biomass OR abund* OR reprod* OR growth OR bioaccum* OR biomagnif* OR photosynth* OR respir* OR metabol*)

Bioaccumulation, bioconcentration, biomagnification - 725 results: (nanopart* OR nanomat*) AND (soil* OR terrestrial* OR aquatic* OR freshwater* OR marine* OR ocean* OR saltwater* OR estuar*) AND (trophic transfer* OR dietary exposure* OR bioaccum* OR biomagnif*)

Description:

Rules for data inclusion:

This is a list of rules we used for excluding particular literature from the analysis and definitions for how additional bioaccumulation/biomagnification metrics were derived from nanomaterial concentration data (in the environment and/or tissues) when those data were available in a paper but the authors did not provide these metrics directly.

-Excluding data:

*If sample size, mean, or standard deviation is not provided then do not record any response ratio data. If no other useful data (e.g. LC50, EC50, BMF, etc) are available, mark paper as excluded.

*If no NM size reported, don’t record the data

*If no control group (i.e. no NM exposure) is present, do not record any response ratio data. If no other useful data (e.g. LC50, EC50, BMF, etc) are available, mark paper as excluded.

*If data are reported as boxplots (with quantile stats instead of mean) don’t record

*If there are treatments with NM exposure in environmental and dietary pathways simultaneously, exclude them from data records

-Rules for calculating trophic transfer factors (formulas from Bour et al., 2015, Journal of Hazardous Materials):

*Bioaccumulation (includes dietary and environmental exposure) = (Concentration in tissue) divided by (Concentration in environment)

*Bioconcentration (environmental exposure only) = (Concentration in tissue) divided by (Concentration in environment)

*Biomagnification (dietary exposure only) = (Concentration in predator) / (Concentration in prey)

People and Organizations

Publishers:

Organization:

Environmental Data Initiative

Email Address:

info@environmentaldatainitiative.org

Web Address:

https://environmentaldatainitiative.org

Creators:

Individual:

James P Guinnip

Organization:

Kansas State University

Email Address:

jguinnip@ksu.edu

Id:

https://orcid.org/0000-0001-8773-6106

Individual:	Walter K Dodds
Organization:	Kansas State University
Id:	https://orcid.org/0000-0002-6666-8930

Individual:	Anne E Schechner
Organization:	Kansas State University
Id:	https://orcid.org/0000-0002-0509-0223

Individual:	Peter J Pfaff
Organization:	Kansas State University
Id:	https://orcid.org/0000-0002-0495-6865

Individual:	Dylan J Smith
Organization:	Kansas State University
Id:	https://orcid.org/0000-0003-0076-0379

Individual:	Hannah I Dea
Organization:	Kansas State University

Individual:	Crosby K Hedden
Organization:	Kansas State University

Individual:	Rachel M Keen
Organization:	Kansas State University

Individual:	Benjamin J Wiens
Organization:	Kansas State University

Contacts:

Individual:

James P Guinnip

Organization:

Kansas State University

Email Address:

jguinnip@ksu.edu

Id:

https://orcid.org/0000-0001-8773-6106

Temporal, Geographic and Taxonomic Coverage

Temporal, Geographic and/or Taxonomic information that applies to all data in this dataset:

Time Period

Begin:

2019-09-01

End:

2019-12-20

Project

Parent Project Information:

Title:

Fate and Toxicity of Engineered Nanomaterials in the Environment: a Meta-analysis

Personnel:

Individual:	James P Guinnip
Id:	https://orcid.org/0000-0001-8773-6106
Role:	Coauthor and data manager

Maintenance

Maintenance:

Description:	There is no ongoing data collection for this project. Maintenance will only occur if users contact the creators about errors.
Frequency:

Other Metadata

Additional Metadata

additionalMetadata
        |___text '\n    '
        |___element 'metadata'
        |     |___text '\n      '
        |     |___element 'unitList'
        |     |     |___text '\n        '
        |     |     |___element 'unit'
        |     |     |     |  \___attribute 'id' = 'year'
        |     |     |     |  \___attribute 'name' = 'year'
        |     |     |     |___text '\n          '
        |     |     |     |___element 'description'
        |     |     |     |___text '\n        '
        |     |     |___text '\n        '
        |     |     |___element 'unit'
        |     |     |     |  \___attribute 'id' = 'unitless'
        |     |     |     |  \___attribute 'name' = 'unitless'
        |     |     |     |___text '\n          '
        |     |     |     |___element 'description'
        |     |     |     |___text '\n        '
        |     |     |___text '\n        '
        |     |     |___element 'unit'
        |     |     |     |  \___attribute 'id' = 'variable'
        |     |     |     |  \___attribute 'name' = 'variable'
        |     |     |     |___text '\n          '
        |     |     |     |___element 'description'
        |     |     |     |     |___text 'refer to NMconcunits column'
        |     |     |     |___text '\n        '
        |     |     |___text '\n        '
        |     |     |___element 'unit'
        |     |     |     |  \___attribute 'id' = 'days'
        |     |     |     |  \___attribute 'name' = 'days'
        |     |     |     |___text '\n          '
        |     |     |     |___element 'description'
        |     |     |     |___text '\n        '
        |     |     |___text '\n      '
        |     |___text '\n    '
        |___text '\n  '

Additional Metadata

additionalMetadata
        |___text '\n    '
        |___element 'metadata'
        |     |___text '\n      '
        |     |___element 'emlEditor'
        |     |        \___attribute 'app' = 'ezEML'
        |     |        \___attribute 'release' = '2021.07.07'
        |     |___text '\n    '
        |___text '\n  '

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Data Package Metadata View Summary

Fate and Toxicity of Engineered Nanomaterials in the Environment: a Meta-analysis

Data Entities

Data Table

Data Package Usage Rights

Keywords

Methods and Protocols

These methods, instrumentation and/or protocols apply to all data in this dataset:

People and Organizations

Temporal, Geographic and Taxonomic Coverage

Project

Parent Project Information:

Maintenance

Additional Metadata

Additional Metadata

Recently Added

Recently Updated

Data Package Metadata View Summary

Fate and Toxicity of Engineered Nanomaterials in the Environment: a Meta-analysis

+/- Data Entities

Data Table

+/- Data Package Usage Rights

+/- Keywords

+/- Methods and Protocols

These methods, instrumentation and/or protocols apply to all data in this dataset:

+/- People and Organizations

+/- Temporal, Geographic and Taxonomic Coverage

+/- Project

Parent Project Information:

+/- Maintenance

+/- Additional Metadata

+/- Additional Metadata

Data Entities

Data Package Usage Rights

Keywords

Methods and Protocols

People and Organizations

Temporal, Geographic and Taxonomic Coverage

Project

Maintenance

Additional Metadata

Additional Metadata