Data Package Metadata View Summary

Extracellular enzyme activities in plots dominated by trees that associate with arbuscular mycorrhizal or ectomycorrhizal fungi in the nitrogen fertilized and reference watershed at the Bear Brook Watershed in Maine, USA during the final year of N fertilization (2016) and during the year after N fertilization ceased (2017).

General Information

Data Package:
Local Identifier:	edi.364.1
Title:	Extracellular enzyme activities in plots dominated by trees that associate with arbuscular mycorrhizal or ectomycorrhizal fungi in the nitrogen fertilized and reference watershed at the Bear Brook Watershed in Maine, USA during the final year of N fertilization (2016) and during the year after N fertilization ceased (2017).
Alternate Identifier:	DOI PLACE HOLDER
Abstract:	Our objective was to detect possible differences in N fertilization responses of extracellular enzymes in plots dominated by trees that associate with arbuscular mycorrhizal fungi (AM) or ectomycorrhizal fungi (ECM). To do this, we established a plot network of 6 AM and 6 ECM dominated (>65% diameter at breast height) 10 x 10 m plots in the lower elevation hardwood zone of both the reference and N fertilized watersheds (N=24 plots) at Bear Brook Watershed, in Maine USA. We assayed the potential activity of hydrolytic enzymes that release N (N-acetylglucosaminidase; NAG), phosphorus (acid phosphatase; AP), and simple carbon (ß-glucosidase; BG). The activities of these enzymes were measured separately in bulk mineral, rhizosphere, and organic horizon soils in the final year of N fertilization at Bear Brook in 2016 and during the year after N fertilization ceased in 2017.
Publication Date:	2021-07-21

Time Period

Begin:

2016-05-01

End:

2017-09-01

People and Organizations
Contact:	Carrara, Joseph E (West Virginia University) [ email ]
Creator:	Carrara, Joseph E (West Virginia University)
Creator:	Fernandez, Ivan J (University of Maine, School of Forest Resources and Climate Change Institute)
Creator:	Brzostek, Edward R (West Virginia University)

Data Entities
Data Table Name:	Bear Brook soil enzyme activities
Description:	Soil extracellular enzyme activities in AM and ECM dominated plots in the N fertilized and reference Watershed at Bear Brook Watershed in Maine USA as measured in 2016 and 2017.

Detailed Metadata

Data Entities

Data Table


Data:	https://pasta-s.lternet.edu/package/data/eml/edi/364/1/453e739c16e7226100a3b3b66c190d53
Name:	Bear Brook soil enzyme activities
Description:	Soil extracellular enzyme activities in AM and ECM dominated plots in the N fertilized and reference Watershed at Bear Brook Watershed in Maine USA as measured in 2016 and 2017.
Number of Records:	144
Number of Columns:	9

Table Structure

Object Name:

BBWM20162017enzymesopen.csv

Size:

11608 bytes

Authentication:

7ebfc395b042a1392a2f4ed7feec4ffb Calculated By MD5

Text Format:

Number of Header Lines:

Record Delimiter:

\r\n

Orientation:

column

Simple Delimited:

Field Delimiter:

Table Column Descriptions

Column Name:

Year

Watershed

Treatment

Mycorrhizae

Plot

SoilFraction

NAG

Definition:

Year the soil samples were taken

Name of the watershed the plot is in

N Fertilized or Reference watershed

Dominant tree mycorrhizal type within each plot

Plot number

Soil type wherein the enzymes were measured

Activity of the enzyme acid phosphatase (phosphorus acquiring enzyme), as micromol AP per gram dry soil per hour

Activity of the enzyme beta-glucosidase (simple carbon acquiring enzyme), as micromol BG per gram dry soil per hour

Activity of the enzyme n-acetylglucosaminidase (nitrogen acquiring enzyme), as micromol NAG per gram dry soil per hour

Storage Type:

date

string

float

Measurement Type:

dateTime

nominal

ratio

Measurement Values Domain:

Format	YYYY
Precision

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	EB
Definition	East Bear
Source

Code Definition

Code	WB
Definition	West Bear
Source

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	N
Definition	N fertilized
Source

Code Definition

Code	Reference
Definition	Reference
Source

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	arbuscular
Definition	arbuscular
Source

Code Definition

Code	ectomycorrhizal
Definition	ectomycorrhizal
Source

Definition

Plot number

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	MineralBulk
Definition	MineralBulk
Source

Code Definition

Code	OrganicHorizon
Definition	OrganicHorizon
Source

Code Definition

Code	Rhizosphere
Definition	Rhizosphere
Source

Unit	micromolePerGramPerHour
Type	real
Min	0.397348007
Max	6.67652212

Unit	micromolePerGramPerHour
Type	real
Min	0
Max	3.440835494

Unit	micromolePerGramPerHour
Type	real
Min	0
Max	3.030818266

Missing Value Code:

Accuracy Report:

Accuracy Assessment:

Coverage:

Methods:

Data Package Usage Rights

This data package is released to the "public domain" under Creative Commons CC0 1.0 "No Rights Reserved" (see: https://creativecommons.org/publicdomain/zero/1.0/). It is considered professional etiquette to provide attribution of the original work if this data package is shared in whole or by individual components. A generic citation is provided for this data package on the website https://portal.edirepository.org (herein "website") in the summary metadata page. Communication (and collaboration) with the creators of this data package is recommended to prevent duplicate research or publication. This data package (and its components) is made available "as is" and with no warranty of accuracy or fitness for use. The creators of this data package and the website shall not be liable for any damages resulting from misinterpretation or misuse of the data package or its components. Periodic updates of this data package may be available from the website. Thank you.

Keywords

By Thesaurus:
(No thesaurus)	nitrogen fertilization, arbuscular mycorrhizal fungi, ectomycorrhizal fungi, extracellular enzymes, Bear Brook Watershed Maine, nitrogen recovery

Methods and Protocols

These methods, instrumentation and/or protocols apply to all data in this dataset:

Methods and protocols used in the collection of this data package

Description:

In order to detect possible differences in N fertilization response of enzyme activities between mycorrhizal types, we established a plot network of 6 AM and 6 ECM dominated (>65% diameter at breast height) 10 x 10 m plots in the lower elevation hardwood zone of both the reference and fertilized watershed of Bear Brook Watershed, Maine in May 2016 (N=24 plots). Tree species were similar between watersheds with AM trees represented by red maple (Acer rubrum) and sugar maple (Acer saccharum) and ECM trees represented by American beech (Fagus grandifolia), grey birch (Betula populifolia), and yellow birch (Betula alleghaniensis).

Description:

To capture variability across the growing season, we sampled soils in each plot in May, July, and September of 2016 and in June, July, and September of 2017. In each plot we extracted three 10 x 10 cm organic horizon layers and homogenized them into a single sample defining this as the OH soil fraction. Next, we sampled four 5 cm diameter mineral soil cores to a depth of 15 cm beneath the OH layer and homogenized these by plot. All samples were kept on ice and transported to West Virginia University for further processing within 48-72 h. Upon return to the lab, we separated rhizosphere soil from mineral soil samples via the soil-adhesion method wherein the rhizosphere soil fraction was operationally defined as soil that remained clung to roots after modest shaking (Phillips & Fahey, 2005). After removal of roots, all soils were passed through a 2 mm sieve and stored at -80C until further analysis.

Description:

To determine the extent to which N fertilization impacts microbial allocation to extracellular enzymes, we assayed the potential activity of hydrolytic enzymes that release N (N-acetylglucosaminidase; NAG), phosphorus (acid phosphatase; AP), and simple carbon (ß-glucosidase; BG). Briefly, 1g of thawed soil was homogenized in 50mM sodium acetate buffer (pH 5.0). Next, hydrolytic activities were determined using a fluorometric microplate assay (Gemini XPS, Molecular Devices, USA). with methylumbelliferone-linked substrates according to Saiya-Cork et al., 2002.

Description:

Saiya-Cork KR, Sinsabaugh RL, Zak DR (2002) The effects of long term nitrogen deposition on extracellular enzyme activity in an Acer saccharum forest soil. Soil Biology and Biochemistry, 34, 1309–1315. DOI: 10.1016/S0038-0717(02)00074-3.

Description:

See protocol: https://allison.bio.uci.edu/protocols/fluorimetricenzymeprotocol.pdf

People and Organizations

Publishers:

Organization:

Environmental Data Initiative

Email Address:

info@environmentaldatainitiative.org

Web Address:

https://environmentaldatainitiative.org

Creators:

Individual:

Joseph E Carrara

Organization:

West Virginia University

Email Address:

jocarrara@mix.wvu.edu

Id:

https://orcid.org/0000-0003-0597-1175

Individual:

Ivan J Fernandez

Organization:

University of Maine, School of Forest Resources and Climate Change Institute

Email Address:

ivanjf@maine.edu

Individual:

Edward R Brzostek

Organization:

West Virginia University

Email Address:

erbrzostek@mix.wvu.edu

Contacts:

Individual:

Joseph E Carrara

Organization:

West Virginia University

Email Address:

jocarrara@mix.wvu.edu

Id:

https://orcid.org/0000-0003-0597-1175

Temporal, Geographic and Taxonomic Coverage

Temporal, Geographic and/or Taxonomic information that applies to all data in this dataset:

Time Period

Begin:

2016-05-01

End:

2017-09-01

Sampling Site:

Description:

Bear Brook Watershed in Maine

Site Coordinates:

Longitude (degree):

-68.106971

Latitude (degree):

44.861781

Project

Parent Project Information:

Title:

Long-Term Research in Environmental Biology (LTREB) program

Personnel:

Individual:

Ivan J Fernandez

Organization:

University of Maine, School of Forest Resources and Climate Change Institute

Email Address:

ivanjf@maine.edu

Role:

Principal Investigator

Funding:

NSF DEB-1119709

Related Project:

Title:

NSF Graduate Research Fellowship to Joseph Carrara

Personnel:

Individual:

Ivan J Fernandez

Organization:

University of Maine, School of Forest Resources and Climate Change Institute

Email Address:

ivanjf@maine.edu

Role:

Principal Investigator

Funding:

NSF DGE-1102

Maintenance

Maintenance:

Description:	completed
Frequency:

Other Metadata

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EDI is a collaboration between the University of New Mexico and the University of Wisconsin – Madison, Center for Limnology:

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Data Table

Data Package Usage Rights

Keywords

Methods and Protocols

These methods, instrumentation and/or protocols apply to all data in this dataset:

People and Organizations

Temporal, Geographic and Taxonomic Coverage

Project

Parent Project Information:

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