# I. Field Collection Methods
## Water Quality
Water quality and environmental parameters are recorded at the start of each sampling event. Water temperature (degrees Celsius), electrical conductivity (microSiemens/cm), specific conductance (microSiemens/cm), pH, dissolved oxygen (mg/L), and turbidity (FNU) are sampled with a YSI ProDSS handheld meter. Turbidity values are averaged over three readings. Secchi depth is measured in the shade. Tide, condition of sampling (condition code), Microcystis level, vegetation rank, direction of flow, and weather are also recorded with water quality parameters.
## Screw Trap
A single rotary screw trap is deployed at levee mile 14.5 near the base of the Yolo Bypass Toe Drain and is typically operated from January 1st through the end of June. If the first flush of the system occurs earlier, the screw trap will be deployed in December to capture out-migrating fishes. The trap site has been selected based on the following criteria for installation, operation, and maintenance: (1) suitable depth: greater than six feet at minimum flow and low tide; (2) suitable velocity: greater than two feet per second (fps) at minimum flow on an ebb tide (though during extremely low flow conditions, the trap may only make a handful of revolutions in a 24-hour period); (3) suitable anchoring points; and, (4) limited public access.
The trap is reached by truck via the Sacramento Deep Water Ship Channel/East Yolo Bypass levee. The trap is accessed daily using a small boat with an outboard motor during high flow or paddles during lower flow periods. During high flows and periods of high debris, the trap may be set intermittently through-out the day, or not set if the high flow becomes a safety concern. The screw trap operates using downstream flow as water strikes the angled baffles on the inside of the trapping cone. This force causes the cone to rotate and fish enter the upstream end of the rotating trapping cone, becoming trapped inside the trapping cone, and then fish are carried rearward into the livebox. Upon checking or pulling the trap fish are then collected using dipnets and transferred to separate buckets. Once all fish have been removed from the livebox, fish are measured to fork length on a wetted measuring board, and then released back into the Toe Drain. Chinook salmon, Smelt species, and other native species are sampled first followed by non-native species. Each fish is identified and counted, and then fork length to the nearest millimeter will be measured for up to 20-50 each species.
The screw trap cone is 8 feet in diameter and designed by EG Solutions (Corvallis, Oregon) (see Figure 1). The trap configuration is as follows: Length: 22.5 ft, Width: 12 ft, Weight: 1300 lbs, Cone: 8 ft diameter and 9 ft length with 2 ft on each end for shaft (total of 13 ft), Livebox: 4 ft by 5 ft with a 25 ft3 volume, Pontoons: 22.5 ft length and 20 in. width, with 9 ft between pontoons. During operation the trap is attached to a ¾” overhead cable originally installed December 2003 (replaced in 2011 after damage during a flood) with two ½” cables attached to each pontoon to align the trap in the center of the channel. The overhead cable is strung between two large trees, each with two ¼” guy cables connected to concrete T anchors. The trap has a rear anchor to maintain the traps position during low flow periods and strong flood tides. A revolution counter was installed on the main shaft of the screw trap in March 2013.
See **DWR-6-SOP-018_v1.1_RotaryScrewTrapSampling** for more information.
## Fyke Trap Sample Collection
The fyke trap is ten feet in diameter and twenty-four feet long with a modified design based on fyke traps operated by the California Department of Fish and Wildlife (CDFW) office in Stockton (see Figure 1). It is constructed out of square stainless steel tubing with galvanized chain link fencing for the main structural netting and black 0.5” square polypropylene fencing lining the terminal compartment to aid in retention and fish safety. The fyke trap is installed with one warning float attached to the downstream end of the trap and one float rigged to the upstream anchoring (nose) cable, trap guide and anchor cables are anchored to t-posts on the levee shoreline, and two guide ropes are also set to help guide the trap when it is rolled up the bank during the retrieval process. The fyke trap doesn’t cover the entire channel as wings are not used, and should not be viewed as a traditional fyke net.
The fyke trap is typically deployed at the beginning of the week, checked daily, and pulled out at the end of the week. Operations are halted briefly in the summer (approximately July-September), when fish catches are low, and are reinitiated in the fall when adult salmon begin migrating up the Toe Drain. When the fyke trap is deployed and operational, fish enter the downstream opening of the trap (10 ft. diameter), pass through the first funnel (3 ft. diameter), and enter a central chamber, then move further through the second funnel (1 ft. 8 in. diameter), and become trapped inside the upstream (terminal) compartment. Captured fish are netted out of the compartment, identified to species, counted, and fork length is measured to the nearest millimeter for up to 20-50 individuals of each species, except for native species of special concern, for which all fish are counted and processed.
See **DWR-6-SOP-017_v1.1_FykeTrapSampling** for more information.
## Beach Seine Sample Collection
Currently, beach seine sites are sampled every-other week with a single haul from a 25 ft by 4 ft (8 x 1.2 meters) pole seine (1/8” sq. mesh). These are modified beach seine hauls; because the levee banks are generally steep, the seine is towed parallel to the shoreline as opposed to netting straight toward the shoreline (see Figure 1).
The seine sites include one perennial pond (YB), nine sites along the Toe Drain (AL# [above Lisbon Weir] and BL# [below Lisbon Weir]) and four high flow sites to capture floodplain inundation periods (RD22, FW1, LIHF, and YB180; only sampled during floodplain inundation).
Generally, during flood events, sampling begins in the northern Bypass, gradually moving southward as the basin drains. Captured fish are identified to species, counted, and fork length is measured to the nearest millimeter for up to 20-50 individuals of each species, except for native species of special concern, for which all fish are counted and processed.
See **DWR-6-SOP-016_v1.1_BeachSeineSampling** for more information.
## Fish Handling of Species of Special Concern
### Salmon:
The handling and sub-sampling protocol for special species caught in the rotary screw trap and beach seines follows California Department of Fish and Wildlife (CDFW) (1997) to minimize impacts to salmon, particularly endangered winter-run Chinook. Juvenile Chinook salmon are visually sorted between winter-run and juveniles of other races based on the Delta Model of daily length-at-date criteria of salvage facility catches (USFWS 1994; modified from Fisher 1992).
Any winter or spring-run sized salmon with a present adipose fin is immediately transferred to a separate bucket and processed for length, weight, and a fin-clip then released. Fall-run sized salmon with a present adipose fin are processed after winter-and-spring run. Any adipose fin-clipped salmon is euthanized and bagged individually (whirl-pak bags) and marked with sampling location, date, gear type, fork length, and time. Fish that are taken either directly or indirectly are then kept on ice in a cooler until transferred to the West Sacramento, CA office to be weighed, processed, and frozen at -20°C.
Captured salmonids are inspected for characters such as presence of yolk sac, parr marks, silvery appearance, and deciduous scales to determine life stage and/or degree of smolting. A simple life stage designation (F, P, or S) is determined for each fish measured:
- Fry (F) = any fish still retaining a yolk sac or not yet fully buttoned-up,
- Parr (P) = anything between a fry and a smolt. The presence of prominent parr marks across their lateral line,
- Smolt (S) = fish starting to become/already are silver in color and shedding scales. Note, smolting fish may still have faded parr marks.
### Osmerids:
During periods of Delta Smelt presence, all Delta Smelt are prioritized and carefully identified to differentiate between Delta (Hypomesus transpacificus) and Wakasagi Smelt (Hypomesus nipponensis). Wakasagi are collected, while Longfin Smelt and Delta Smelt are released.
Identification is made based upon:
- Chromatophores: Chromatophores refer to the dot pigmentation on the isthmus. Wakasagi will have two or more, Delta Smelt will have one or none. Juvenile Wakasagi, however, may be missing chromatophores.
- Caudal peduncle pigmentation: This refers to pigmentation near the leading, anterior edge of the caudal fin. On Wakasagi, this area will consist of scattered spots whereas Delta Smelt will have a smaller number of spots that are clustered around the caudal fin in a V-shape.
### Native Species:
Native Cyprinids and Trout are scanned for PIT tags using a PIT tag reader. If the reader detects a tag, the tag number is written down and information is submitted to relevant IEP Project Work Teams.
Other fishes
Following the processing of special species (salmon and smelt) all other fish >25mm in fork length are identified and counted: fork length is measured to the nearest millimeter on a wetted measuring board for up to 20-50 of each species. Occasionally, whole samples or fin clips from other fish species are collected for special studies or genetic identification. Any juvenile fish that cannot be field identified are preserved on ice with respective labels for individual identification and examined at the West Sacramento, CA office.
See **DWR-6-SOP-008_v1.3_ListedSpeciesHandling** for more information.
# II. Sample Processing and Tracking
### Post-Processing
- All genetic fin clips are stored in 95% ethanol and sent to contractors for genetic identification.
- CWT tags of ad-clipped salmon are extracted and read by YBMFP staff and reported in the online Regional Mark Information System (RMIS). See DWR-6-SOP-003_v1.1_CodedWireTagRecovery for more information.
- Diet samples, which are occasionally sampled for special studies, are prepared by YBFMP staff (removal of stomach, fixed in formalin) and sent to contractors for identification of contents.
- Whole fish heads (Otoliths) are kept for special studies.
- Otoliths can be stored a few recommended ways. If the otoliths are clean and completely free of fish parts, then they can be stored dry. Otoliths can be frozen (limiting the freeze/thaw action) or preserved in EtOH greater than 70%
- Osmerids collected are examined under a microscope for laboratory identification, photographed, and a fin clip is collected and sent to contractors for genetic ID. See **DWR-6-SOP-008_v1.3_ListedSpeciesHandling** for more information.
### Sample Tracking
Samples are tracked on a spreadsheet both for permitting reasons and to maintain a list of genetic samples. A chain of custody (COC) listing sample number, date, time, location, type, and study/project is sent to contractors, who check that all samples are accounted for. Signatures are required of both the person responsible for sending the sample package, and the person receiving it. Once the sample is sent, the contractor is notified of approximate date of delivery.
### Contractor
Genetic identification of fishes is conducted by the Genomic Variation Laboratory at the University of California Davis. One contract covers Chinook salmon run-type, while the other covers genetic identification of Osmerids and other fish species (varies depending on special studies).
Genetic identification of Chinook salmon run-type:
- Location: 2403 Meyer Hall, Department of Animal Science, UC Davis. One Shields Avenue, Davis, CA 95616
- Lab phone: 530-752-6351
- PI contact: Mariah Meek mhmeek@msu.edu
- Lab Director: Andrea D. Schreier amdrauch@ucdavis.edu
- Main technician contact: Emily Funk (ecfunk@ucdavis.edu)
Genetic identification of Osmerids and other fishes:
- Location: 2403 Meyer Hall, Department of Animal Science, UC Davis. One Shields Avenue, Davis, CA 95616
- Lab phone: 530-752-6351
- PI contact: Amanda Finger ajfinger@ucdavis.edu
- Main technician contact: Emily Funk (ecfunk@ucdavis.edu)
- Additional technician contact: Mary Badger (mebadger@ucdavis.edu)
Diet contents associated with historical fish studies were identified and enumerated by the Wetland Ecology Team at the University of Washington.
- Location: 1122 N.E. Boat Street, Seattle, WA, 98195
- Phone: 206-543-7532
- Contact: Jeff Cordell jcordell@uw.edu
## III. Quality Assurance and Control
Four levels of quality control are conducted on data:
1. Field data are checked by someone other than the data recorder prior to leaving each field site,
2. Datasheets are checked while being entered into the Microsoft Access database, which has customized error-checking and data validation checks,
3. A separate DWR staff member compares data from original field sheets to data entered into the database,
4. Finally, data are sorted and/or summarized to highlight erroneous outliers. In addition to data sorting, qualitative analyses are conducted on the water quality data by creating multi-layered visualizations of data in R-studio including; boxplots, histograms, and regressions of correlated water quality parameters from sampling sites across time. Outlier tests were also run on water quality data and used to identify suspicious data. See **Fish_Publication_QAQC_Workflow_v1.0** for more details.
*Notes on Data Quality and Usage:*
Dataset Structure:
- YBFMP is now publishing in the tidy format (separate tables similar to the way they are organized in the relational database) in order to follow best data practices of reducing duplication of data in any given table. This reduces errors and file size and helps with clarity for how data are sampled and organized. See Fish_Workflow_Tables.pdf for the organizational structure published here, and the keys used to join tables. More information and resources can be provided by the IEP Data Utilization Workgroup (see https://iep.ca.gov/Data/Data-Utilization-Working-Group).
- We have included a flat file of environmental/water quality and catch data for those who are not familiar with joining tables, or using R, but have also included instructions in integrate_fish_data.Rmd for customizing the dataset (e.g. for seeing length data with water quality data, seeing salmon data only, smelt data only) using different joins.
Water Quality:
- We have flagged some of the water quality data based on visual inspection of outliers, and outlier tests. See Flag_WQ and Comment_WQ columns for flags. To remove any flagged data, see line 86 in integrate_fish_data.Rmd.
Rotary Screw Trap:
- When ESA listed fish are caught, some operational flexibility is required, for example shorter day-time only sets are fished when we near the lethal take limit for Delta Smelt, winter-run Chinook salmon, and Steelhead/Rainbow Trout (Oncorhynchus mykiss). High debris loads can also mandate shorter sets, which have been as short as one hour in some instances. Volume cannot be measured because there has not always been a flow-meter in place; effort is generally reported in trap operation hours. However, “set”, “check” and “pull” dates and times were not always accurately reported, and therefore operation hours must be calculated as an estimate using the fish capture events in some years. Due to less consistent tracking and inconsistencies of sampling effort prior to 2010, best estimated hours of sampling are summarized on a monthly scale prior to 2010. Daily estimates are provided starting in 2010.
- The screw trap cone only spins when water is flowing downstream. This means that the trap does not samples as efficiently when net flow is reverse of the cone during flood tides or downstream flow is slower. Using hours for catch-per-unit effort may be useful for long term trends but is hard to compare on the daily scale due to these flow differences. The revolution counter data, starting in 2013, helps account for this flow effect.
Fyke Trap:
- Effort is generally reported in trap operation hours, which is calculated based on fyke “set”, “check” and “pull” dates and times. While these were relatively well reported in comparison with the rotary screw trap, there may be instances where these were not correctly reported.
Rotary Screw Trap and Beach Seine:
- Based on a subset of samples that were genetically identified, species identification of minnows and basses <30mm may not be reliable to the species level.
We began to identify all killifish as “Killifish” starting August 9, 2021 because this was the first date we genetically identified some of our killifish as Bluefin Killifish. After comparing field photos with genetic IDs, we decided we could not accurately speciate between Rainwater and Bluefin Killifish in the field, and thus combined the two species into “Killifish.” When using the data, please note that there is a good chance a portion of Rainwater Killifish prior to this date are also Bluefin Killifish, but we do not have confirmation and have thus left these as Rainwater Killifish.
## IV. Archiving
### Sample Archive
All salmon, smelt, and fish associated with special studies are kept in the YBMFP lab freezer, sorted by year, species, and run (if applicable). There is no defined storage period but samples are typically archived until no longer usable. Fish brought back from the field for identification are often disposed of after their identification is confirmed. More information is available in Sample Archiving Guide.
### Data Management and Archiving
Field data are collected and recorded on paper datasheets by DWR personnel, scanned weekly, then entered into a Microsoft Access database. The monitoring program is currently transitioning to a new database called WISKI, which will replace Microsoft Access by the end of 2023. Paper datasheets are archived in binders that are stored at the West Sacramento DWR office, and electronic copies are archived on DWR/AEU Network drives.
Genetic results are received from the contractor via email in an Excel spreadsheet. Data are printed and entered into the Access database by DWR personnel. Hard copies of the data are stored in binders at the West Sacramento DWR office. Electronic copies of results for taxonomic analyses are archived on DWR/AEU Network drives. Salmon genetic results are also entered into a separate Excel spreadsheet.
## V. Calculations and Analysis
### Catch Per Unit Effort (CPUE) Calculations
**Rotary Screw Trap and Fyke Trap:**
CPUE = Count/Time
-or-
CPUE = Count/Revolutions
where Time can be months, days, or hours.
Revolutions are only available for screw trap data from 2013-current.
See “Notes on Data Quality” for more information.
**Beach Seine:**
CPUE = Count/Volume
Volume = length * width * height
Note: height is calculated by averaging the water height at each end of the beach seine net.
## VI. Historical Changes
### Equipment
**Physical Water Quality:** Prior to 2011, YSI 63, and possibly others. Between 2011-October 2016, a YSI 556 was used. Starting October 2016, a YSI ProDSS was used.
**Turbidity:** February 2012-October 2016: Glass vials of water were collected and analyzed with Hach 2100Q portable turbidimeter. Starting in October 2016, the YSI ProDSS was used.
**Screw Trap:** 1998: a 5 ft rotary screw trap (RTSR5) was used for one less than month in January and February during high flows after which the 8 ft screw trap (RSTR8) was used.
**Fyke Trap:** (1) in 1998 and 1999 there were two traps fishing four months a year and seven days a week, (2) in 2001 a second trap was deployed, (3) in 2002 there was a design change in the fyke trap and three fyke traps were deployed, (4) between 2003 and 2006 the trap was check every-other day instead of daily, and (5) in 2014 and 2015 clogging by water hyacinth rendered the trap likely ineffective for some period and the trap was out of operation and then relocated (from October to December).
**Beach Seine:** Before October of 2012, a 50ft by 4ft beach seine was used to conduct sampling. From October 2012 to present, a 25ft by 4ft beach seine has been used.
### Field Sampling
#### Rotary Screw trap
Rotary screw trap (RSTR8) operation began in 1998, deployment generally occurs from December/January through June, and that period of months has been sampled relatively consistently every year of the monitoring program. Throughout the history of the program there have sometimes been two rotary screw traps in place (1998 and 1999), the screw trap has been installed one month early to capture winter flooding events (December 2012), and operational challenges have augmented its sampling frequency (see below for specific changes).
2000-2009: the rotary screw trap was often set over weekends and checked every 1-2 days
2010: the operation of the trap was changed to daily checks and only set on weekends during some flooding events
2011: Damage due to high debris loads resulted in several weeks of no operation in late March and April
2012: High catches of ESA listed species resulted in shorter daytime only sets intermittently from January through March
2016: High catches of ESA listed species resulted in shorter daytime only sets intermittently for one month (March 29th through April 26th)
2017: Short day-time only sets intermittently from January to May due to high flows
2019: Short day-time only sets intermittently from February to May due to high flows
2020-2021: Not sampled from 3/18/20 through 3/7/2021 due to COVID-19 pandemic.
#### Fyke trap:
1998: During the first year of the program and a high flow year, a Fyke net was deployed intermittently off the Yolo Bypass Causeway bridge from January to February. This method was removed from the program after 1998, after which only the Fyke trap was used, 1999-2018
2000 – 2009: the high number of sampling hours were due to the fyke trap often being fished over weekends and checked every 1-2 days
2002: Conductivity starts being collected
May 2008: Specific Conductivity, pH, Dissolved Oxygen start being collected
2010: operation of the fyke trap changed to daily checks with no weekend fishing in 2010
February 2012: Turbidity starts being collected
2014: clogging by water hyacinth likely reduced trap efficiency for some period and the trap was out of operation and then temporarily relocated to a downstream location (Alt_Fyke). Trap was not operated October 10th to November 13th
2015: clogging by water hyacinth likely reduced trap efficiency for some period and the trap was out of operation and then temporarily relocated to a downstream location (Alt_Fyke). Trap not operated from December 1st to 10th.
2016: trap was not operated in November for bank reconstruction
Late October 2016-present: Turbidity readings taken by YSI instead of Hach turbidimeter.
2017: trap was operated intermittently during high flood times.
2020: Not sampled from 3/18/20–10/14/2020 due to COVID-19 pandemic.
2021: Not sampled from 12/7/2020-3/2/2021 due to COVID-19 pandemic. Not sampled 10/25/2021-10/29/2021 due to historic rainfall causing large amounts of vegetation and flow that made it unsafe to deploy Fyke Trap.
#### Beach Seine:
1998-1999: additional seining efforts during a high flood year were conducted in addition to the 50 ft seine (SEINE50), such as SEIN30 (30 ft), SEINE100 (100 ft), SEINCOVE (beach seine cove set), SEINENCL (beach seine enclosure), and PSEIN100 (100 ft purse seine).
1998-2006: additional sampling was conducted during floodplain inundation and drainage events.
2002: seining effort was reduced to what it is today in order to help provide a long-term database on fish use of the basin. Conductivity starts being collected.
2007: additional sites were sampled in the Cache Slough complex.
2008: additional sampling was conducted during floodplain inundation and drainage events, not including sites in the Cache Slough complex.
May 2008: Specific Conductivity, pH, Dissolved Oxygen start being collected.
2010: the additional above Lisbon Weir (AL 1-4) and below Lisbon (BL 1-5) were added as year-round sampling to provide better spatial and temporal data on fish assemblages within the Yolo Bypass Toe Drain.
2011-2012: additional sampling was conducted during floodplain inundation and drainage events.
February 2012: Turbidity starts being collected.
2015: Site BL6 was added because of difficulty sampling BL5 in 2012, 2013, and 2014 due to clogging from water hyacinth (e.g. complete site coverage).
Late October 2016-present: Turbidity readings taken by YSI instead of Hach turbidimeter.
2018: AL-2 was removed based on analyses of AL sites showing homogenous fish catches across sites during both wet and dry years (report available upon request), and AL1 was moved 30 m upstream due to erosion during the 2017 flood.
2019: Added vegetation rank to field data sheets.
Sept. 2019: Changed the name of the Cache Creek Sinks (CCS1-7) sites to the Toe Drain at Road 22 (RD22) for the high flow beach seine site.
2020: Not sampled from 3/18/20 - 4/19/21 due to COVID-19 pandemic.
8/9/2021: Beach seine length standardized to 30m for all sites.
1998-present:
In 1998, 1999, 2000, 2001, 2002, 2003, 2004, 2005, 2006, 2008, 2011, 2012, 2013, 2015, 2016, 2017, 2018, and 2019 additional sampling was conducted during floodplain inundation and drainage events.
Throughout the history of the program there have been three perennial pond sites:
Yolo Basin Wetlands “study pond”, located next the tree grove at I-80 Causeway (YB),
a Fremont Weir wetlands pond, located approximately 1 mile south of the weir along the east levee (FW pond), and
Sacramento Bypass (scour pond and large earthen pond at south levee) (SW). Historically, depending on pond size, 1-3 three standard "U.S. Fish and Wildlife-style" (e.g. perpendicular) beach seine hauls would be performed at random coordinates around the perimeter of each pond site.
#### All:
2019: added vegetation rank to field data sheets.
10/25/2021: added SampleAltered, FlowDirection variables to data sheets.
#### Genetic Sampling
2016: Sampling fin clips for genetic analysis began to help identify the different runs of salmon present in the Yolo Bypass and allow for more accurate federal and state take reporting. Genetics on YBFMP salmon were also analyzed from 2012-2016, though this data was collected for and belongs to the Ecological Restoration Program (CDFW).
2011: Genetic sampling (via fin clip or swab) of smelt began.
2018-2019: Genetic sampling (via fin clip) of Hitch was conducted for a special study.
2018: Genetic sampling (via fin clip) of Sacramento Blackfish began.
2019: Genetic sampling (via fin clip) of Lamprey began.
2021: Genetic subsampling (via fin clip) of Killifish began in August to differentiate between Rainwater and Bluefin Killifish.
See DWR-6-MET-007_Genetics_v1.2 for additional information.
#### Coded Wire Tag Sampling
2016: CWTs began to be processed and read by YBFMP staff. Prior to this they were sent out externally for processing.
## VII. Review Processes
QA/QC processes are described in Methods. No other review is conducted.
## VIII. Methods References
|Reference Location or DOI| Reference Title |
| ----------- | ----------- |
|https://github.com/AEU-DISE/publish_fish/tree/main/metadata/methods_references|DWR-6-SOP-003_v1.1_CodedWireTagRecovery|
|https://github.com/AEU-DISE/publish_fish/tree/main/metadata/methods_references|DWR-6-SOP-006_v1.1_LaboratoryDissectionofFish|
|https://github.com/AEU-DISE/publish_fish/tree/main/metadata/methods_references|DWR-6-SOP-008_v1.3_ListedSpeciesHandling|
|https://github.com/AEU-DISE/publish_fish/tree/main/metadata/methods_references|DWR-6-SOP-016_v1.1_BeachSeineSampling|
|https://github.com/AEU-DISE/publish_fish/tree/main/metadata/methods_references|DWR-6-SOP-017_v1.1_FykeTrapSampling|
|https://github.com/AEU-DISE/publish_fish/tree/main/metadata/methods_references|DWR-6-SOP-018_v1.1_RotaryScrewTrapSampling|
|https://github.com/AEU-DISE/publish_fish/tree/main/metadata/methods_references|DWR-6-MET-007_Genetics_v1.2|
|https://github.com/AEU-DISE/publish_fish/tree/main/metadata/methods_references|Fish_Publication_QAQC_Workflow_v1.0|
