These methods, instrumentation and/or protocols apply to all data in this dataset:Methods and protocols used in the collection of this data package |
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Description: |
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Experiment Design
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The BAC experiment uses a subset of 38 plots from the Biodiversity II (e120) experiment which began in 1994. See: Tilman et al. (2001) Diversity and productivity in a long-term grassland experiment. Science 294, 843?845. doi:10.1126/science.1060391 Biodiversity II is contained within a block of 342 plots laid out in a grid. Each plot was established as a 13 m x 13 m square, but only the central 9 m x 9 m is actively maintained to contain the specified species and level of plant diversity.
BAC plots consist of fourteen monoculture plots, nine plots planted to 4 species, nine plots planted to 16 species and six plots planted to 32 species. Plant species are from 4 different functional groups (4 species, each, of C3 grasses, C4 grasses, legumes and non-legume forbs) that are likely to respond differently to changes in temperature and water availability. The diversity by warming treatments are a fully factorial split-plot design, with each of high, low, and control heat treatment subplots nested within each of 1, 4, 16 and 32 species diversity treatment whole-plots.
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Warming Treatments
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Warming treatments are applied to 2.5 x 3 m subplots via infrared lamps (Kalglo Electronics,
Bethlehem, PA, USA). The three warming treatments are control (metal shade above plot to simulate shading effects of lamps), low heat (600 W lamp), and high heat (1200 W lamp). Pre-experiment bare-ground trials of the infrared lamps indicated that the low heat treatment would approximately warm the soil by +1.5C while the high heat treatment would exhibit an approximate warming of +3C. Lamps are turned on from March to November.
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Heat Lamps
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Kalglo Electronics, Bethlehem, PA, USA
High Intensity: Model MRM-2412 240V w/ mod ref 1200W 5.0A 50/60Hz 1Ph 30’ cord
Ser.#: 20071771
Low Intensity: Model MRM-2406 240V w/ mod ref 600W 2.5A 50/60Hz 1Ph 50’ cord
Ser.#: 20071651
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Diversity Treatments
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The diversity levels are maintained via hand weeding multiple times each growing season from scaffolding above the plots to avoid trampling. Plots are burned annually to mimic the natural fire regime of the prairie. Because of this, fall biomass harvests are good indicators of seasonal net primary productivity.
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Abstract
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Climate changes forecast for our region by GCM???s and shifts in biodiversity and composition each have the potential to alter ecosystem functioning; their interactive effects are unknown. The "BAC" experiment is designed to determine the direct and interactive effects of plant species numbers, plant community composition, temperature, and precipitation on 11 productivity, C and N dynamics, stability, and plant, microbe, and insect species abundances in CDR grassland ecosystems.
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