Diffusive fluxes for methane and carbon dioxide were collected using the floating chamber method (Galfalk et al. 2013; McClure et al. 2020). Following McClure et al. 2020, the flux chamber was constructed using an opaque bucket fitted with foam and wrapped with reflective aluminum tape to prevent internal heating. The volume of the bucket was 0.02 meters cubed with an area of 0.15 meters squared. The fitted foam allowed the chamber to sit approximately 3 cm below the water surface and sealed the inside of the chamber from the surrounding air. The chamber was connected to a Los Gatos ultraportable GHG analyzer (UGGA: Los Gatos Research Inc., San Jose, CA, USA) through two air-tight gas ports which were fitted to the top of the chamber and connected to two separate three-meter sections of 0.635-cm Tygon PVC tubing before being connected to the inlet and waste valves on the UGGA. Air was circulated through the chamber at approximately 405 mL per minute. The moisture corrected methane and carbon dioxide (ppm) were recorded every 10 seconds. The chamber was vented until atmospheric concentrations were reached before being lowered to the water’s surface where 5 minutes of data were collected. This was repeated twice at each site for two replicate measurements per sampling occurence.
Diffusive fluxes (methane, carbon dioxide) were calculated from the collected UGGA concentrations using the FluxCalR package (Zhao, 2019). Briefly, peaks for methane concentration were visually identified for each day and each replicate and served as the end time point for slope calculation (CO2 and CH4, respectively). The slope of carbon dioxide/methane concentration was then calculated for 4 minutes prior to the identified end time point. The slope was converted to diffusive flux assuming 1 atmosphere of pressure and averaging the ambient temperature over the entire measurement period.
References:
Galfalk, M., Bastviken, D., Fredriksson, S., & Arneborg, L. (2013). Determination of the piston velocity for water‐air interfaces using flux chambers, acoustic Doppler velocimetry, and IR imaging of the water surface. J. Geophys. Res. Biogeosci., 118(2), 770-782. https://doi.org/10.1002/jgrg.20064
McClure, R.P., M.E. Lofton, S. Chen, K.M. Krueger, J.C. Little, and C.C. Carey. 2020. Methane ebullition and diffusion rates, turbulence, water temperature, and water depth data from Falling Creek Reservoir (Virginia, USA) in the ice-free period during 2016-2019 ver 2. Environmental Data Initiative. https://doi.org/10.6073/pasta/c6b1a54e356f5ab093d86dcdb89177ba
Zhao, J (2019). FluxCalR: a R package for calculating CO2 and CH4 fluxes from static chambers. Journal of Open Source Software, 4(43), 1751, https://doi.org/10.21105/joss.01751
Additional notes:
From 2018-2022, multiple whole-ecosystem oxygen manipulations were conducted at Falling Creek Reservoir. For a detailed description of the hypolimnetic oxygenation engineered system, see Gerling et al. (2014). These systems were operated over time following the SSS inflow file in Carey et al. (2021).
Carey, C. C., R. Q. Thomas, R. P. McClure, A. G. Hounshell, W. M. Woelmer, H. L. Wander, & A. S. L. Lewis. (2021). CareyLabVT/FCR-GLM: FCR GLM-AED model, data, and code for Carey et al. manuscript (v1.0). Zenodo. https://doi.org/10.5281/zenodo.5528865
Gerling, A.B., Browne, R.G., Gantzer, P.A., Mobley, M.H., Little, J.C., and C.C. Carey. 2014. First report of the successful operation of a side stream supersaturation hypolimnetic oxygenation system in a eutrophic, shallow reservoir. Water Research. 67: 129-143. doi: 10.1016/j.watres.2014.09.002