Samples were collected weekly at an established sampling location at each station. Sampling locations were chosen based on adequate concentration of flow, proximity to weir and staff gage, and low vulnerability to disturbance. Samples were collected and stored in polyethylene bottles.
Weekly analyses included nitrate, phosphate, total nitrogen, total phosphorus, chloride and sulfate.
Samples for anion and cation analysis were filtered (0.45 micron).
Every six weeks, samples were shipped to the Cary Institute of Ecosystem Studies (CIES) for anion, cation, TN, and TP analysis.
One blank from the laboratory distilled water source was prepared each week and stored along with the samples. Spike analysis was done as part of the routine long-term BES stream sampling and analysis. Samples were stored at 4 degrees C.
Concentrations of nitrate, chloride and sulfate were analyzed on a Dionex LC20 series ion chromatograph. Concentrations of phosphate were analyzed on a Lachat Quikchem 8000 flow injection analyzer.
Total nitrogen and phosphorus were analyzed by persulfate digestion followed by analysis of nitrate and phosphate on a Lachat Quikchem 8000 flow injection analyzer. If the value for total N was more than 0.10 mg N L-1 less than nitrate-N, the total N value for that sample was set to the nitrate value. If the value for total P was more than 0.01 mg P L-1 less than the phosphate P, the total P value for that sample was set to the phosphate value.