Data Package Metadata View Summary

Algal Nutrient Limitation Bioassays in Sycamore Creek, Arizona, USA (19xx-20xx)

General Information

Data Package:
Local Identifier:	knb-lter-cap.590.4
Title:	Algal Nutrient Limitation Bioassays in Sycamore Creek, Arizona, USA (19xx-20xx)
Alternate Identifier:	DOI PLACE HOLDER
Abstract:	The primary objective of this project is to understand how long-term climate variability and change influence the structure and function of desert streams via effects on hydrologic disturbance regimes. Climate and hydrology are intimately linked in arid landscapes; for this reason, desert streams are particularly well suited for both observing and understanding the consequences of climate variability and directional change. Researchers try to (1) determine how climate variability and change over multiple years influence stream biogeomorphic structure (i.e., prevalence and persistence of wetland and gravel-bed ecosystem states) via their influence on factors that control vegetation biomass, and (2) compare interannual variability in within-year successional patterns in ecosystem processes and community structure of primary producers and consumers of two contrasting reach types (wetland and gravel-bed stream reaches). This dataset addresses patterns of nutrient limitation by primary producers as indicated by in situ growth on artificial substrates.
Publication Date:	2020-08-23

Time Period

Begin:

2010-05-15

End:

2020-05-21

People and Organizations
Contact:	Information Manager (Central Arizona–Phoenix LTER) [ email ]
Creator:	Grimm, Nancy (Arizona State University)
Creator:	Sabo, John (Arizona State University)
Creator:	Bonjour, Sophia (Arizona State University)
Creator:	Dong, Xiaoli (University of California, Davis)
Creator:	Handler, Amalia (USEPA National Health and Environmental Effects)
Creator:	Kemmitt, Katherine (Wenck)
Creator:	Lauck, Marina (Arizona State University)
Creator:	Palta, Monica (Pace University)
Creator:	Pollard, Lindsey (Arizona State University)

Data Entities
Data Table Name:	590_nutrient_diffusing_substrata_fa70c9a52cdf9b19a09feb11ad21e93c.csv
Description:	nutrient diffusion assays to assess nutrient limitation of primary producers in wetland and gravel sites of Sycamore Creek, AZ

Detailed Metadata

Data Entities

Data Table


Data:	https://pasta-s.lternet.edu/package/data/eml/knb-lter-cap/590/4/dc9cc7f4055a9c97b0a98ad08c4d9136
Name:	590_nutrient_diffusing_substrata_fa70c9a52cdf9b19a09feb11ad21e93c.csv
Description:	nutrient diffusion assays to assess nutrient limitation of primary producers in wetland and gravel sites of Sycamore Creek, AZ
Number of Records:	360
Number of Columns:	14

Table Structure

Object Name:

590_nutrient_diffusing_substrata_fa70c9a52cdf9b19a09feb11ad21e93c.csv

Size:

39913 bytes

Authentication:

fa70c9a52cdf9b19a09feb11ad21e93c Calculated By MD5

Text Format:

Number of Header Lines:

Record Delimiter:

\r\n

Orientation:

column

Simple Delimited:

Field Delimiter:	,
Quote Character:	"

Table Column Descriptions

Column Name:

Date_retrieved

Site

Days_in_field

Treatment

Extract

Extract_vol_ml

ABS_750

ABS_666

ABS_480

ABS_750a

ABS_666a

Chl_mg/m2

Pheo_mg/m2

Flags

Definition:

Date cups were recovered from field incubations

Location of field incubation

Days of field incubation

Agar treatment used: Control- agar only, NO3- agar and sodium nitrate, PO4- agar and potasium phosphate monobasic, NO3+PO4- agar, sodium nitrate, and potasium phosphate monobasic. A cation study was done in 2015 and included sodium phosphate monobasic

Either ethanol or methanol was used

Volume of solvent (methanol) used in exctraction

Background corrected absorbance measurement on spectrophotometer at wavelength 750 nm. Negative values were changed to zero

Background corrected absorbance measurement on spectrophotometer at wavelength 666 nm, 665 nm was used for ethanol extractions

Background corrected absorbance measurement on spectrophotometer at wavelength 480 nm

Background corrected absorbance measurement on spectrophotometer at wavelength 750 nm after acidification. Negative values were changed to zero

Background corrected absorbance measurement on spectrophotometer at wavelength 666 nm after acidification, 665 nm was used for ethanol extractions

Concentration of chlorophyll a

Concentration of pheophytin a

notes or problems encountered

Storage Type:

date

string

float

string

float

string

Measurement Type:

dateTime

nominal

ratio

nominal

ratio

nominal

Measurement Values Domain:

Format	YYYY-MM-DD
Precision

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	SSG
Definition	DosS gravel reach
Source

Code Definition

Code	SSW
Definition	DosS wetland reach
Source

Unit	nominalDay
Type	natural
Min	13
Max	50

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	Control
Definition	agar only
Source

Code Definition

Code	NaP
Definition	agar and sodium phosphate monobasic
Source

Code Definition

Code	NO3
Definition	agar and sodium nitrate
Source

Code Definition

Code	NO3+NaP
Definition	agar sodium nitrate and sodium phosphaphate monobasic
Source

Code Definition

Code	NO3+PO4
Definition	agar sodium nitrate and potasium phosphate monobasic
Source

Code Definition

Code	PO4
Definition	agar and potasium phosphate monobasic
Source

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	Ethanol
Definition	Ethanol
Source

Code Definition

Code	Methanol
Definition	Methanol
Source

Unit	milliliter
Type	natural
Min	15
Max	200

Unit	nanometer
Type	real
Min	-0.009
Max	0.038

Unit	nanometer
Type	real
Min	-0.002
Max	0.876

Unit	nanometer
Type	real
Min	-0.001
Max	0.42

Unit	nanometer
Type	real
Min	-0.007
Max	0.11

Unit	nanometer
Type	real
Min	-0.003
Max	0.538

Unit	milligramsPerSquareMeter
Type	real
Min	-11.485359
Max	875.950083

Unit	milligramsPerSquareMeter
Type	real
Min	-161.713862
Max	411.635284

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	Negative Chloro
Definition	Negative Chloro
Source

Code Definition

Code	Negative phaeo
Definition	Negative phaeo
Source

Code Definition

Code	Weird
Definition	Weird
Source

Missing Value Code:

Code	NA
Expl	missing value

Code	NA
Expl	missing value

Code	NA
Expl	missing value

Accuracy Report:

Accuracy Assessment:

Coverage:

Methods:

Data Package Usage Rights

This data package is released to the "public domain" under Creative Commons CC0 1.0 "No Rights Reserved" (see: https://creativecommons.org/publicdomain/zero/1.0/). The consumer of these data ("Data User" herein) has an ethical obligation to cite it appropriately in any publication that results from its use. The Data User should realize that these data may be actively used by others for ongoing research and that coordination may be necessary to prevent duplicate publication. The Data User is urged to contact the authors of these data if any questions about methodology or results occur. Where appropriate, the Data User is encouraged to consider collaboration or coauthorship with the authors. The Data User should realize that misinterpretation of data may occur if used out of context of the original study. While substantial efforts are made to ensure the accuracy of data and associated documentation, complete accuracy of data sets cannot be guaranteed. All data are made available "as is". The Data User should be aware, however, that data are updated periodically and it is the responsibility of the Data User to check for new versions of the data. The data authors and the repository where these data were obtained shall not be liable for damages resulting from any use or misinterpretation of the data. Thank you.

Keywords

By Thesaurus:
LTER controlled vocabulary	phosphate, phosphorus, photosynthesis, nitrogen, nutrients, diatoms, phaeophytin, chlorophyll, chlorophyll a, algae, deserts, floods, aquatic ecosystems, disturbance, disturbance patterns, disturbance properties, disturbances, diversity, drainage, droughts, streams
LTER core areas	population studies, disturbance patterns, water and fluxes
Creator Defined Keyword Set	sycamore creek, sonoran desert, LTREB
CAPLTER Keyword Set List	cap lter, cap, caplter, central arizona phoenix long term ecological research, arizona, az, arid land

Methods and Protocols

These methods, instrumentation and/or protocols apply to all data in this dataset:

Methods and protocols used in the collection of this data package

Description:

# Procedure 1. See Tank et al. 2006 Nutrient Diffusing Substrata (NDS) method. 2. Four agar treatments (control, +NaNO3, KH2PO4, + NaNO3 & KH2PO4) were prepared and poured into labeled Polycon cups (Maden Plastic's) with pre-drilled lids. Fritted glass disks with diameter of 2.7 cm were placed on top of agar and lids securely shut. 3. Cups were attached randomly to L-bars using silicone caulk and different colored zip-ties. One color was designated for each treatment to ensure sample recognition after incubation. 4. L-bars were wrapped in plastic wrap and kept in the refrigerator for no more than 36 hours. 5. L-bars were secured to the stream bed parallel to streamflow using tent stakes. 6. Disks were allowed to accumulate biofilm in the stream. Incubation time is indicated by "Days_in_field" (at least 20 days if field is blank). 7. Chlorophyll a and pheophytin a were analyzed using a hot methanol extraction method (Tett et al. 1975, Tett et al. 1977). Absorbance values were measured using a Beckman DU® 530 Spectrophotometer. # Calculations - Negative 750 nm turb numbers were changed to zero - All absorbance values were background corrected - Chl a (mg/m2) = (219.2 \times v \times ((ABS_666-ABS_750)-(ABS_666a-ABS_750a)))/A - Pheophytin a(mg/m2) = (219.2 \times v ((ABS_666a-ABS_750a \times 3.8)-(ABS_666-ABS_750)))/A - 219.2 is the absorption coefficient for chlorophyll a in methanol \times 10 for conversion from ug/cm2 to mg/m2 - v is the extraction volume of solvent in mL - A is area of fritted glass disk, 5.7255 cm2 - 3.8 is the maximum ratio of (ABS_666-ABS_750): (ABS_666a-ABS_750a) in the absence of pheopigments # References - Tank, J., Bernot, M., & Rosi-Marshall, E. 2006. Nitrogen limitation and uptake. Pg 213-236 in Hauer, F. R. Lamberti, G. A. (Eds.) Methods in stream ecology. 2nd edition. Academic Press, San Diego, CA. - Tett, P., M. G. Kelly, and G. M. Hornberger. 1975. A method for the spectrophotometric measurement of chlorophyll and pheophytin a in benthic microalgae. Limnology and Ocreanography 20:887-896. - Tett, P., M. G. Kelly, and G. M. Hornberger. 1977. Estimation of chlorophyll a and pheophytin a in methanol. Limnology and Oceanography 22:579-580.

People and Organizations

Publishers:

Organization:

Central Arizona–Phoenix LTER

Address:

Arizona State University,

Global Institute of Sustainability,

Tempe, AZ 85287-5402 USA

Creators:

Individual:

Nancy Grimm

Organization:

Arizona State University

Email Address:

nbgrimm@asu.edu

Id:

https://orcid.org/0000-0001-9374-660X

Individual:

John Sabo

Organization:

Arizona State University

Email Address:

John.L.Sabo@asu.edu

Id:

https://orcid.org/0000-0001-5259-0709

Individual:

Sophia Bonjour

Organization:

Arizona State University

Email Address:

sbonjour@asu.edu

Id:

https://orcid.org/0000-0003-3614-7023

Individual:

Xiaoli Dong

Organization:

University of California, Davis

Email Address:

xldong@ucdavis.edu

Id:

https://orcid.org/0000-0003-3303-0735

Individual:

Amalia Handler

Organization:

USEPA National Health and Environmental Effects

Email Address:

Handler.amalia@Epa.gov

Id:

https://orcid.org/0000-0001-8372-6488

Individual:

Katherine Kemmitt

Organization:

Wenck

Email Address:

kkemmitt@wenck.com

Id:

https://orcid.org/0000-0002-3794-8941

Individual:

Marina Lauck

Organization:

Arizona State University

Email Address:

marina.lauck@asu.edu

Id:

https://orcid.org/0000-0002-9032-0782

Individual:

Monica Palta

Organization:

Pace University

Email Address:

mpalta@pace.edu

Individual:

Lindsey Pollard

Organization:

Arizona State University

Email Address:

ldpollar@gmail.com

Contacts:

Organization:

Central Arizona–Phoenix LTER

Position:

Information Manager

Address:

Arizona State University,

Global Institute of Sustainability,

Tempe, AZ 85287-5402 USA

Email Address:

caplter.data@asu.edu

Web Address:

https://sustainability.asu.edu/caplter/

Metadata Providers:

Individual:

Nancy Grimm

Organization:

Arizona State University

Email Address:

nbgrimm@asu.edu

Id:

https://orcid.org/0000-0001-9374-660X

Individual:

Lindsey Pollard

Organization:

Arizona State University

Email Address:

ldpollar@gmail.com

Individual:

Sophia Bonjour

Organization:

Arizona State University

Email Address:

sbonjour@asu.edu

Id:

https://orcid.org/0000-0003-3614-7023

Temporal, Geographic and Taxonomic Coverage

Temporal, Geographic and/or Taxonomic information that applies to all data in this dataset:

Time Period

Begin:

2010-05-15

End:

2020-05-21

Geographic Region:

Description:

gravel reach of the Dos S (SS) sampling site of Sycamore Creek in central Arizona, USA

Bounding Coordinates:

Northern:	33.750482	Southern:	33.749021
Western:	-111.507645	Eastern:	-111.506849

Geographic Region:

Description:

wetland reach of the Dos S (SS) sampling site of Sycamore Creek in central Arizona, USA

Bounding Coordinates:

Northern:	33.748620	Southern:	33.74835
Western:	-111.510883	Eastern:	-111.50779

Project

Parent Project Information:

Title:

Central Arizona–Phoenix Long-Term Ecological Research Project

Personnel:

Individual:

Daniel Childers

Organization:

Arizona State University

Email Address:

dan.childers@asu.edu

Id:

https://orcid.org/0000-0003-3904-0803

Role:

Principal Investigator

Individual:

Nancy Grimm

Organization:

Arizona State University

Email Address:

nbgrimm@asu.edu

Id:

https://orcid.org/0000-0001-9374-660X

Role:

Co-principal Investigator

Individual:

Sharon Hall

Organization:

Arizona State University

Email Address:

sharonjhall@asu.edu

Id:

https://orcid.org/0000-0002-8859-6691

Role:

Co-principal Investigator

Individual:

Billie Turner II

Organization:

Arizona State University

Email Address:

Billie.L.Turner@asu.edu

Id:

https://orcid.org/0000-0002-6507-521X

Role:

Co-principal Investigator

Individual:

Abigail York

Organization:

Arizona State University

Email Address:

Abigail.York@asu.edu

Id:

https://orcid.org/0000-0002-2313-9262

Role:

Co-principal Investigator

Abstract:

Phase IV of the Central Arizona-Phoenix LTER (CAP) continues to focus on the question: How do the ecosystem services provided by urban ecological infrastructure (UEI) affect human outcomes and behavior, and how do human actions affect patterns of urban ecosystem structure and function and, ultimately, urban sustainability and resilience? The overarching goal is to foster social-ecological urban research aimed at understanding these complex systems using a holistic, ecology of cities perspective while contributing to an ecology for cities that enhances urban sustainability and resilience. This goal is being met through four broad programmatic objectives: (1) use long-term observations and datasets to articulate and answer new questions requiring a long-term perspective; (2) develop and use predictive models and future-looking scenarios to help answer research questions; (3) employ existing urban ecological theory while articulating new theory; and (4) build transdisciplinary partnerships to foster resilience and enhance sustainability in urban ecosystems while educating urban dwellers of all ages and experiences. CAP IV research is organized around eight interdisciplinary questions and ten long-term datasets and experiments, and researchers are organized into eight Interdisciplinary Research Themes to pursue these long-term research questions.

Funding:

NSF Awards: CAP I: DEB-9714833, CAP II: DEB-0423704, CAP III: DEB-1026865, CAP IV: DEB-1832016

Additional Award Information:

Funder:

National Science Foundation

Funder ID:

https://ror.org/021nxhr62

Number:

1832016

Title:

LTER: CAP IV - Investigating urban ecology and sustainability through the lens of Urban Ecological Infrastructure

URL:

https://nsf.gov/awardsearch/showAward?AWD_ID=1832016&HistoricalAwards=false

Related Project:

Title:

Multiscale effects of climate variability and change on hydrologic regimes, ecosystem function, and community structure in a desert stream and its catchment

Personnel:

Individual:

Nancy Grimm

Organization:

Arizona State University

Email Address:

nbgrimm@asu.edu

Id:

https://orcid.org/0000-0001-9374-660X

Role:

Principal Investigator

Individual:

John Sabo

Organization:

Arizona State University

Email Address:

john.l.sabo@asu.edu

Id:

https://orcid.org/0000-0001-5259-0709

Role:

Co-Principal Investigator

Abstract:

The primary objective of this project is to understand how long-term climate variability and change, as expressed through hydrologic regime shifts, influence the structure and function of desert streams. Desert streams are well suited for observing the consequences of climate variability because they experience high hydrologic variability at multiple scales. Researchers will determine how regime shifts influence 1) large-scale stream biogeomorphic structure (i.e., prevalence and persistence of wetlands) over multiple years via their influence on factors that control vegetation biomass, and 2) within-year successional patterns in ecosystem processes and community structure of primary producers and consumers of reaches along a gradient of wetland presence and stability. Arid regions are characterized by high interannual variation in precipitation, and these climate patterns drive the overall disturbance regime (in terms of flooding and drying), which influences the geomorphic structure and nutrient status of desert stream ecosystems. Embedded within the multi-annual hydrologic regime, flash floods scour stream channels and initiate a series of rapid successional changes by benthic algae, aquatic and wetland plants, and macroinvertebrates at short time scales (i.e., within a year); patterns of succession are hypothesized to vary among years within the long-term hydrologic regime. The research will use new techniques developed by the team to analyze the relationships between hydrologic variability, hydrologic regime shifts, and ecosystem and community properties.

Funding:

- NSF SUCCESSION-I 1977-1979 DEB-7724478 (Fisher) - NSF SUCCESSION-II 1980-1983 DEB-8004145 (Fisher) - NSF SUCCESSION-III 1984-1987 BSR-8406891 (Fisher) - NSF Postdoc 1987-1989 BSR 87-00122 (Grimm) - NSF STABILITY - 1989-1992 BSR-8818612 (Fisher and Grimm) - NSF TROPHIC STRUCTURE 1990-1992 BSR-9008114 (Fisher, Grimm, and Dudley) - NSF LTREB I - 1991-1996 DEB-9108362 (Grimm and Fisher) - NSF HETEROGENEITY - 1993-1998 DEB-9306909 (Fisher and Grimm) - EPA HYPORHEIC - 1994-1996 #R821250-01-0 (Fisher and Grimm) - NSF LINX I - 1996-1999 - DEB-9628860 (subaward to Grimm, Marti, and Fisher) - NSF LTREB II - 1996-2001 DEB-9615358 (Grimm and Fisher) - NSF LINX II - 2001-2006 DEB-0111410 (subaward to Grimm and Dahm) - NSF LTREB III - 2009-2015 DEB-0918262 (Grimm and Sabo) - NSF LTREB IV - 2015-2020 DEB-1457227 (Grimm and Sabo) - NSF LINKAGES - 1998-2000 DEB-9727311 (Fisher and Grimm) - NSF DDIG - 1998-1999 DEB-9800912 (Dent and Grimm)

Additional Award Information:

Funder:

National Science Foundation

Funder ID:

https://ror.org/021nxhr62

Number:

1457227

Title:

LTREB Renewal: Multiscale effects of climate variability and change on hydrologic regimes, ecosystem function, and community structure in a desert stream and its catchment

URL:

https://nsf.gov/awardsearch/showAward?AWD_ID=1457227&HistoricalAwards=false

Other Metadata

Copyright 2024 Environmental Data Initiative. This material is based upon work supported by the National Science Foundation under grants #2223103 and #2223104. Any opinions, findings, conclusions, or recommendations expressed in the material are those of the author(s) and do not necessarily reflect the views of the National Science Foundation. Please contact us with questions, comments, or for technical assistance regarding this web site or the Environmental Data Initiative. Please read our privacy policy to know what information we collect about you and to understand your privacy rights.

EDI is a collaboration between the University of New Mexico and the University of Wisconsin – Madison, Center for Limnology:

Data Package Metadata View Summary

Algal Nutrient Limitation Bioassays in Sycamore Creek, Arizona, USA (19xx-20xx)

Data Entities

Data Table

Data Package Usage Rights

Keywords

Methods and Protocols

These methods, instrumentation and/or protocols apply to all data in this dataset:

People and Organizations

Temporal, Geographic and Taxonomic Coverage

Project

Parent Project Information:

Recently Added

Recently Updated

Data Package Metadata View Summary

Algal Nutrient Limitation Bioassays in Sycamore Creek, Arizona, USA (19xx-20xx)

+/- Data Entities

Data Table

+/- Data Package Usage Rights

+/- Keywords

+/- Methods and Protocols

These methods, instrumentation and/or protocols apply to all data in this dataset:

+/- People and Organizations

+/- Temporal, Geographic and Taxonomic Coverage

+/- Project

Parent Project Information:

Data Entities

Data Package Usage Rights

Keywords

Methods and Protocols

People and Organizations

Temporal, Geographic and Taxonomic Coverage

Project