LOCATION DESCRIPTIONS
Fine Root samples were collected at 2 different locations.
Watershed 1 Sites:
These sites are located along the W1 trail at the elevations provided below. There are ten collectors per site arranged along two transects, with five 0.097 m2 collectors on each transect. Collections on W1 began in 1997.
Low, hardwood – elevation 520 m
Mid, hardwood – elevation 600 m
Upper, hardwood – elevation 710 m
High, spruce/fir – elevation 740 m
Bear Brook Sites:
West of W6: 0.097 m2 collectors are randomly situated along three 100 m long transects (325 degrees) which are approximately 25 m apart. Originally 20 collectors per elevation were set out; there are currently twelve per elevation at all but the high elevation where fifteen remain.
Low, hardwood - southwest of W6 weir, west of tower platform - elevation 525 m
Mid, hardwood - west of Throughfall collector sites - elevation 585 m
Upper, hardwood - west of tower site and seep - elevation 725 m
High, hardwood - west of rain gauge 9 - elevation 830 m
SAMPLING DESIGN
In August 1998 (prior to Ca treatment on WS1) soil cores were collected adjacent to
each of the 36 litterfall traps in WS1 and in the Bear Brook reference forest plots. A split PVC
corer of 5 cm diameter was hammered into the soil to a depth of 20 cm as
measured from the top of the mineral soil. Locations occupied by boulders and
root crowns were avoided. Soil cores were divided in the field into four layers:
forest floor organic horizon plus 0-5 cm mineral soil and 5-10, 10-15, and 15-20
cm mineral soil. The same field procedure was repeated in August
2013, fourteen years after treatment, with cores again paired with litterfall traps.
Soil samples were returned to the laboratory and stored frozen until processed
for root biomass. Roots were hand sorted from soil cores into two diameter
classes (< 1 mm, 1-2 mm); live roots were recognized by color and tensile strength and
retained for dry weight determination. Roots were dried to constant mass at 70
degrees celcius and weighed to +/- 0.001 g. The fine root biomass data were computed on a grams
per m2 basis (forest floor plus 0-20 cm mineral soil depth) after taking into account the
estimated area of each sampling plot that was occupied by boulders and root crowns and
hence was not sampled by root coring.
A line intercept method was applied to determine this fraction. (Fahey et al. 2005).