Data Package Metadata View Summary

Hubbard Brook Experimental Forest: microbial biomass and activity at climate gradient plots, 2010-2012

General Information

Data Package:
Local Identifier:	knb-lter-hbr.197.3
Title:	Hubbard Brook Experimental Forest: microbial biomass and activity at climate gradient plots, 2010-2012
Alternate Identifier:	knb-lter-hbr.197
Alternate Identifier:	DOI PLACE HOLDER
Abstract:	In October 2010, as part of a study of the relationships between snow depth, soil freezing and nutrient cycling (http://www.ecostudies.org/people_sci_groffman_snow_summary.html), we established 6 20 x 20 m plots (intensive plots) and 14 10 x 10-m plots (extensive plots) following an elevation gradient, with eight of the plots facing north and twelve facing south. Seasonal (spring, summer autumn) measurements of soil nitrate (NO₃-) and ammonium (NH₄+) concentrations, microbial biomass carbon (C) and nitrogen (N) content, microbial respiration, potential nitrification and N mineralization rates, and denitrification potential occurred from 2010 – 2012. These data were gathered as part of the Hubbard Brook Ecosystem Study (HBES). The HBES is a collaborative effort at the Hubbard Brook Experimental Forest, which is operated and maintained by the USDA Forest Service, Northern Research Station.
Short Name:	Microbial Biomass Climate Gradient
Publication Date:	2020-01-31
Language:	English

Time Period

Begin:

2010-11-19

End:

2012-08-07

People and Organizations
Contact:	Information Manager (Hubbard Brook Ecosystem Study) [ email ]
Creator:	Groffman, Peter M
Creator:	Martel, Lisa
Creator:	Duran, Jorge

Data Entities
Data Table Name:	Hubbard Brook Climate Gradient Microbial Biomass and Activity
Description:	Hubbard Brook Climate Gradient Microbial Biomass and Activity

Detailed Metadata

Data Entities

Data Table


Data:	https://pasta-s.lternet.edu/package/data/eml/knb-lter-hbr/197/3/f6a818339216aa8ad7174fe7bf5d370e
Name:	Hubbard Brook Climate Gradient Microbial Biomass and Activity
Description:	Hubbard Brook Climate Gradient Microbial Biomass and Activity
Number of Records:	300
Number of Columns:	17

Table Structure

Object Name:

HubbardBrook_ClimateGradient_MicrobialBiomass_2010-2012.csv

Size:

33313 byte

Authentication:

b8db0ae9a6e79de912786173e6a85329 Calculated By MD5

Text Format:

Number of Header Lines:

Record Delimiter:

\r\n

Orientation:

column

Simple Delimited:

Field Delimiter:	,
Quote Character:	"

Table Column Descriptions

Project

Date

Year

Season

Treatment

Elevation

Sample

Horizon

BIOC

RESPC

BION

NO3

NH4

NIT

MIN

DEA

H2O

Column Name:

Project

Date

Year

Season

Treatment

Elevation

Sample

Horizon

BIOC

RESPC

BION

NO3

NH4

NIT

MIN

DEA

H2O

Definition:

Project name

Sample date

Year of sample collection

Season

Treatment

Elevation

Sample location

Soil horizon

Microbial biomass

Soil respiration rate

Microbial biomass N

Soil nitrate

Soil ammonium

Potential net nitrification

Potential net N mineralization

Denitrification enzyme activity

Gravimetric water content gram water Per gram wet soil

Storage Type:

string

date

string

float

Measurement Type:

nominal

dateTime

nominal

ratio

Measurement Values Domain:

Definition

any text

Format	YYYY-MM-DD
Precision

Format	YYYY
Precision

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	Su
Definition	Summer
Source

Code Definition

Code	Sp
Definition	Spring
Source

Code Definition

Code	W
Definition	Winter
Source

Code Definition

Code	F
Definition	Fall
Source

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	NA
Definition	Treatment not applicable to gradient study
Source

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	NA
Definition	NA
Source

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	IH3
Definition	Intensive High Elevation Plot 3
Source

Code Definition

Code	IH2
Definition	Intensive High Elevation Plot 2
Source

Code Definition

Code	IH1
Definition	Intensive High Elevation Plot 1
Source

Code Definition

Code	IL3
Definition	Intensive Low Elevation Plot 3
Source

Code Definition

Code	IL2
Definition	Intensive Low Elevation Plot 2
Source

Code Definition

Code	IL1
Definition	Intensive Low Elevation Plot 1
Source

Code Definition

Code	E14
Definition	Extensive Plot 14
Source

Code Definition

Code	E13
Definition	Extensive Plot 13
Source

Code Definition

Code	E12
Definition	Extensive Plot 12
Source

Code Definition

Code	E11-B
Definition	Extensive Plot 11-B
Source

Code Definition

Code	E11
Definition	Extensive Plot 11
Source

Code Definition

Code	E10
Definition	Extensive Plot 10
Source

Code Definition

Code	E9
Definition	Extensive Plot 9
Source

Code Definition

Code	E8
Definition	Extensive Plot 8
Source

Code Definition

Code	E7
Definition	Extensive Plot 7
Source

Code Definition

Code	E6
Definition	Extensive Plot 6
Source

Code Definition

Code	E5
Definition	Extensive Plot 5
Source

Code Definition

Code	E4
Definition	Extensive Plot 4
Source

Code Definition

Code	E3
Definition	Extensive Plot 3
Source

Code Definition

Code	E2
Definition	Extensive Plot 2
Source

Code Definition

Code	E1
Definition	Extensive Plot 1
Source

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	O
Definition	he entire O horizon: Oi, Oe, and Oa combined
Source

Code Definition

Code	Min
Definition	The first 10 cm of mineral soil below the A horizon (E and/or B)
Source

Code Definition

Code	Oa/A
Definition	Oa and A horizons combined
Source

Code Definition

Code	Oi/Oe
Definition	Oi and Oe horizons combined
Source

Unit	milligramPerKilogram
Type	real

Unit	milligramPerKilogramPerDay
Type	real

Unit	milligramPerKilogram
Type	real

Unit	milligramPerKilogram
Type	real

Unit	milligramPerKilogram
Type	real

Unit	milligramPerKilogramPerDay
Type	real

Unit	milligramPerKilogramPerDay
Type	real

Unit	microgramPerKilogramPerHour
Type	real

Unit	gramPerGram
Type	real

Missing Value Code:

Code	-9999.99
Expl	Data missing or not taken at this time

Code	-9999.99
Expl	Data missing or not taken at this time

Code	-9999.99
Expl	Data missing or not taken at this time

Code	-9999.99
Expl	Data missing or not taken at this time

Code	-9999.99
Expl	Data missing or not taken at this time

Code	-9999.99
Expl	Data missing or not taken at this time

Code	-9999.99
Expl	Data missing or not taken at this time

Code	-9999.99
Expl	Data missing or not taken at this time

Code	-9999.99
Expl	Data missing or not taken at this time

Accuracy Report:

Accuracy Assessment:

Coverage:

Methods:

Data Package Usage Rights

This information is released under the Creative Commons license - Attribution - CC BY (https://creativecommons.org/licenses/by/4.0/). The consumer of these data ("Data User" herein) is required to cite it appropriately in any publication that results from its use. The Data User should realize that these data may be actively used by others for ongoing research and that coordination may be necessary to prevent duplicate publication. The Data User is urged to contact the authors of these data if any questions about methodology or results occur. Where appropriate, the Data User is encouraged to consider collaboration or co-authorship with the authors. The Data User should realize that misinterpretation of data may occur if used out of context of the original study.

While substantial efforts are made to ensure the accuracy of data and associated documentation, complete accuracy of data sets cannot be guaranteed. All data are made available "as is." The Data User should be aware, however, that data are updated periodically and it is the responsibility of the Data User to check for new versions of the data. The data authors and the repository where these data were obtained shall not be liable for damages resulting from any use or misinterpretation of the data. Thank you.

Keywords

By Thesaurus:
Hubbard Brook Ecosystem Study	climate gradient, Hubbard Brook LTER
LTER Network Controlled Vocabulary	ammonium, carbon, denitrification, microbes, microbial activity, microbial biomass, microbial nitrogen, mineralization, nitrate, nitrification, nitrogen, respiration, soil
LTER Network Core Research Areas	inorganic nutrients, primary production

Methods and Protocols

These methods, instrumentation and/or protocols apply to all data in this dataset:

Methods and protocols used in the collection of this data package

Description:

LOCATION DESCRIPTION

In October 2010, we selected 20 independent plots (separated by >300 m) 10 m diameter plots along an elevation gradient that spanned 375 – 775 m asl. This gradient encompasses a range in mean annual temperature of ~2.5 oC that is similar to the change projected to occur with climate change over the next 50-100 years in northeastern North America (Hayhoe et al., 2007). There was relatively little variation in soil types with all plots being located on well to moderately well-drained soils dominated by typic Haplorthods. The vegetation in the plots was characteristic of northern hardwood forests and was dominated by sugar maple (Acer saccharum) along the gradient (Schwarz et al., 2003).

SAMPLING DESIGN

Samples were collected in November 2010, May 2011, Aug 2011, March 2012 and August 2012. Samples were collected with a split- PVC corer. A split PVC corer consists of a piece of 2 inch (5 cm) PVC pipe, about 15-20 cm long, split lengthwise on both sides. The corer is actually in two pieces. We put the corer together along the cuts, and duct-tape one side -- the "hinge" side. Holding the corer firmly together, we hammer it 10-15cm into the ground. The corer is removed and then opened with the intact soil core inside. The soil is split into three layers (Oi/Oe, Oa/A, and mineral horizons). Each horizon is measured and placed into a sample bag. We typically collect 2-8 cores per site, compositing all cores by horizon.

DATA DESCRIPTION

Oi and Oe horizons were composited into one sample, as were Oa and A horizons. Mineral samples generally consist of the top 10 cm of mineral soil beginning below the A horizon.

LABORATORY PROCEDURES

Samples were stored at 4^oC between sampling and analysis (from less than 1 week to up to three weeks). Soils were manually homogenized: all large rocks, roots, and other non-decomposed organic material were removed, and samples were thoroughly mixed. No more than three minutes were spent homogenizing any sample. All samples were held at field moisture before analysis. Soil water content was determined gravimetrically.

Microbial biomass C and N content was measured using the chloroform fumigation-incubation method (Jenkinson and Powlson 1976). Soils were fumigated to kill and lyse microbial cells in the sample. The fumigated sample was inoculated with fresh soil and sealed in a jar, and microorganisms from the fresh soil grew vigorously using the killed cells as substrate. The flushes of carbon dioxide (CO₂) and 2 M KCl extractable inorganic N (NH₄+ and NO₃-) released by the actively growing cells during a 10-day incubation at field moisture content were assumed to be directly proportional to the amount of C and N in the microbial biomass of the original sample. A proportionality constant (0.41) was used to calculate biomass C from the CO₂ flush in the fumigated samples. Biomass N is the total inorganic N flush in the fumigated samples.

Inorganic N and CO₂ production were also measured in "control" samples. Control samples were prepared in the same fashion as those listed above, but were not fumigated. These incubations provided estimates of microbial respiration and potential net N mineralization and nitrification. Microbial respiration was quantified from the amount of CO₂ evolved over the 10-day incubation. Potential net N mineralization and nitrification were quantified from the accumulation of NH₄+ plus NO₃- and NO₃- alone during the 10-day incubation. We measured 2 M KCl extractable inorganic N in the fresh soil samples to determine the initial soil NO₃- and NH₄+ concentrations. Carbon dioxide was measured by thermal conductivity gas chromatography. Inorganic N was measured colorometerically using an autoanalyzer (Lachat Quikchem 8100).

Denitrification enzyme activity was measured using the short-term anaerobic assay described by Smith and Tiedje (1979). Sieved soils were amended with NO3- (100 mg N kg^-1), glucose (40 mg kg^-1), chloramphenicol (10 mg kg^-1) and acetylene (10 kPa) and were incubated under anaerobic conditions for 90 minutes. Gas samples were taken at 30 and 90 minutes, stored in evacuated glass tubes and analyzed for N₂O by electron capture gas chromatography. For more information on any of the methods described above, refer to Standard Soil Methods for Long-Term Ecological Research (1999).

CALCULATIONS

All results are expressed on a per gram of dry soil basis. Values can be converted to a “per area” basis using data on the mass of different soil horizons found elsewhere on the data page of this website.

REFERENCES

People and Organizations

Publishers:

Organization:

Hubbard Brook Ecosystem Study

Address:

234 Mirror Lake Road,

North Woodstock, NH 03262 United States

Creators:

Individual:

Peter M Groffman

Email Address:

GroffmanP@caryinstitute.org

Id:

https://orcid.org/0000-0001-8371-6255

Individual:

Lisa Martel

Email Address:

martell@caryinstitute.org

Id:

https://orcid.org/0000-0002-0156-4954

Individual:

Jorge Duran

Contacts:

Organization:

Hubbard Brook Ecosystem Study

Position:

Information Manager

Address:

234 Mirror Lake Road,

North Woodstock, NH 03262 United States

Email Address:

hbr-im@lternet.edu

Web Address:

https://hubbardbrook.org

Temporal, Geographic and Taxonomic Coverage

Temporal, Geographic and/or Taxonomic information that applies to all data in this dataset:

Time Period

Begin:

2010-11-19

End:

2012-08-07

Sampling Site:

Description:

IL1: Intensive Low Elevation Plot 1. Aspect: South. UTM: X= 282144, Y= 4869478

Site Coordinates:

Longitude (degree):

-71.71479424

Latitude (degree):

43.94617659

Sampling Site:

Description:

IL2: Intensive Low Elevation Plot 2. Aspect: South. UTM: X= 281685, Y= 4869704

Site Coordinates:

Longitude (degree):

-71.72060047

Latitude (degree):

43.94807308

Sampling Site:

Description:

IL3: Intensive Low Elevation Plot 3. Aspect: South. UTM: X= 280887, Y= 4869966

Site Coordinates:

Longitude (degree):

-71.73064174

Latitude (degree):

43.95019236

Sampling Site:

Description:

IH1: Intensive High Elevation Plot 1. Aspect: North. UTM: X= 277790, Y= 4868286

Site Coordinates:

Longitude (degree):

-71.7684914

Latitude (degree):

43.93415543

Sampling Site:

Description:

IH2: Intensive High Elevation Plot 2. Aspect: North. UTM: X= 277888, Y= 4868064

Site Coordinates:

Longitude (degree):

-71.76717913

Latitude (degree):

43.93218854

Sampling Site:

Description:

IH3: Intensive High Elevation Plot 3. Aspect: North. UTM: X= 278314, Y= 4867892

Site Coordinates:

Longitude (degree):

-71.76180633

Latitude (degree):

43.93077007

Sampling Site:

Description:

E1: Extensive Plot 1. Aspect: North. UTM: X=277410, Y=4867546

Site Coordinates:

Longitude (degree):

-71.77291084

Latitude (degree):

43.92738579

Sampling Site:

Description:

E2: Extensive Plot 2. Aspect: North. UTM: X= 277111, Y= 4867074

Site Coordinates:

Longitude (degree):

-71.77643367

Latitude (degree):

43.92305067

Sampling Site:

Description:

E3: Extensive Plot 3. Aspect: North. UTM: X= 276485, Y= 4850906

Site Coordinates:

Longitude (degree):

-71.77745584

Latitude (degree):

43.91985666

Sampling Site:

Description:

E4: Extensive Plot 4. Aspect: North. UTM: X= 277986, Y= 4867455

Site Coordinates:

Longitude (degree):

-71.76570567

Latitude (degree):

43.92674122

Sampling Site:

Description:

E5: Extensive Plot 5. Aspect: North. UTM: X= 278055, Y= 4866940

Site Coordinates:

Longitude (degree):

-71.76463237

Latitude (degree):

43.92213059

Sampling Site:

Description:

E6: Extensive Plot 6. Aspect: North. UTM: X= 281632, Y= 4870329

Site Coordinates:

Longitude (degree):

-71.72151804

Latitude (degree):

43.95368099

Sampling Site:

Description:

E7: Extensive Plot 7. Aspect: South. UTM: UTM: X= 281869, Y= 4870458

Site Coordinates:

Longitude (degree):

-71.71862125

Latitude (degree):

43.95490408

Sampling Site:

Description:

E8: Extensive Plot 8. Aspect: South. UTM: X= 281785, Y= 4870899

Site Coordinates:

Longitude (degree):

-71.71984434

Latitude (degree):

43.95885229

Sampling Site:

Description:

E9: Extensive Plot 9. Aspect: South. UTM: X= 281650, Y= 4871108

Site Coordinates:

Longitude (degree):

-71.72161276

Latitude (degree):

43.96068944

Sampling Site:

Description:

E10: Extensive Plot 10. Aspect: South. UTM: X= 281068, Y= 4870840

Site Coordinates:

Longitude (degree):

-71.72874928

Latitude (degree):

43.95810127

Sampling Site:

Description:

E11: Extensive Plot 11. Abandonded in Fall 2011 and replaced with 11-B. Aspect: South. UTM: X= 279820, Y= 4870325

Site Coordinates:

Longitude (degree):

-71.74407265

Latitude (degree):

43.95310239

Sampling Site:

Description:

E11-B: Extensive Plot 11-B. Replacement for E11 started in Fall 2011. Aspect: South. UTM: X= 279957, Y= 4870590

Site Coordinates:

Longitude (degree):

-71.74247222

Latitude (degree):

43.95552778

Sampling Site:

Description:

E12: Extensive Plot 12. Aspect: South. UTM: X= 290093, Y= 4870317

Site Coordinates:

Longitude (degree):

-71.74066667

Latitude (degree):

43.95311111

Sampling Site:

Description:

E13: Extensive Plot 13. Aspect: South. UTM: X= 280252, Y= 4870034

Site Coordinates:

Longitude (degree):

-71.73857446

Latitude (degree):

43.95061447

Sampling Site:

Description:

E14: Extensive Plot 14. Aspect: South. UTM: X= 281277, Y= 4870177

Site Coordinates:

Longitude (degree):

-71.72587194

Latitude (degree):

43.95220301

Project

Other Metadata

Additional Metadata

additionalMetadata
        |___text '\n    '
        |___element 'metadata'
        |     |___text '\n      '
        |     |___element 'unitList'
        |     |     |___text '\n        '
        |     |     |___element 'unit'
        |     |     |        \___attribute 'abbreviation' = 'g/g'
        |     |     |        \___attribute 'id' = 'gramPerGram'
        |     |     |        \___attribute 'name' = 'gramPerGram'
        |     |     |        \___attribute 'unitType' = 'massPerMass'
        |     |     |___text '\n        '
        |     |     |___element 'unit'
        |     |     |     |  \___attribute 'abbreviation' = 'ug/kg/hour'
        |     |     |     |  \___attribute 'id' = 'microgramPerKilogramPerHour'
        |     |     |     |  \___attribute 'multiplierToSI' = '0.0001'
        |     |     |     |  \___attribute 'name' = 'microgramPerKilogramPerHour'
        |     |     |     |  \___attribute 'parentSI' = 'gram'
        |     |     |     |  \___attribute 'unitType' = 'massPerMassRate'
        |     |     |     |___text '\n          '
        |     |     |     |___element 'description'
        |     |     |     |     |___text 'micrograms per kilogram per hour'
        |     |     |     |___text '\n        '
        |     |     |___text '\n        '
        |     |     |___element 'unit'
        |     |     |     |  \___attribute 'abbreviation' = 'mg/Kg'
        |     |     |     |  \___attribute 'id' = 'milligramPerKilogram'
        |     |     |     |  \___attribute 'multiplierToSI' = '0.0001'
        |     |     |     |  \___attribute 'name' = 'milligramPerKilogram'
        |     |     |     |  \___attribute 'parentSI' = 'gram'
        |     |     |     |  \___attribute 'unitType' = 'mass'
        |     |     |     |___text '\n          '
        |     |     |     |___element 'description'
        |     |     |     |     |___text 'amount of substance per mass'
        |     |     |     |___text '\n        '
        |     |     |___text '\n        '
        |     |     |___element 'unit'
        |     |     |     |  \___attribute 'abbreviation' = 'mg/kg/day'
        |     |     |     |  \___attribute 'id' = 'milligramPerKilogramPerDay'
        |     |     |     |  \___attribute 'multiplierToSI' = '0.0001'
        |     |     |     |  \___attribute 'name' = 'milligramPerKilogramPerDay'
        |     |     |     |  \___attribute 'parentSI' = 'gram'
        |     |     |     |  \___attribute 'unitType' = 'massPerMassRate'
        |     |     |     |___text '\n          '
        |     |     |     |___element 'description'
        |     |     |     |     |___text 'milligrams per kilogram per hour'
        |     |     |     |___text '\n        '
        |     |     |___text '\n      '
        |     |___text '\n    '
        |___text '\n  '

Copyright 2024 Environmental Data Initiative. This material is based upon work supported by the National Science Foundation under grants #2223103 and #2223104. Any opinions, findings, conclusions, or recommendations expressed in the material are those of the author(s) and do not necessarily reflect the views of the National Science Foundation. Please contact us with questions, comments, or for technical assistance regarding this web site or the Environmental Data Initiative. Please read our privacy policy to know what information we collect about you and to understand your privacy rights.

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Data Package Metadata View Summary

Hubbard Brook Experimental Forest: microbial biomass and activity at climate gradient plots, 2010-2012

Data Entities

Data Table

Data Package Usage Rights

Keywords

Methods and Protocols

These methods, instrumentation and/or protocols apply to all data in this dataset:

People and Organizations

Temporal, Geographic and Taxonomic Coverage

Project

Additional Metadata

Recently Added

Recently Updated

Data Package Metadata View Summary

Hubbard Brook Experimental Forest: microbial biomass and activity at climate gradient plots, 2010-2012

+/- Data Entities

Data Table

+/- Data Package Usage Rights

+/- Keywords

+/- Methods and Protocols

These methods, instrumentation and/or protocols apply to all data in this dataset:

+/- People and Organizations

+/- Temporal, Geographic and Taxonomic Coverage

+/- Project

+/- Additional Metadata

Data Entities

Data Package Usage Rights

Keywords

Methods and Protocols

People and Organizations

Temporal, Geographic and Taxonomic Coverage

Project

Additional Metadata