Samples were split into organic (composite of Oi, Oe, and Oa soil
horizons) and mineral (generally the top 6-10 cm of A soil horizon)
soils. Samples were collected prior to any icing treatment in
September 2015, and then for 2 years after the icing treatments were
initially applied (1st icing: 1/27/16; 2nd icing, only in MIDx2 plots:
1/14/17) in June 2016, August 2016, October 2016, May 2017, August
2017, and November 2017 (i.e. spring, summer, and fall of 2016 and
2017).
Samples were stored at 4°C between sampling and analysis (from less
than 1 week to up to 3 weeks). Soils were manually homogenized: all
large rocks, roots, and other non-decomposed organic material were
removed, and samples were removed, and samples were thoroughly mixed.
All samples were held at field moisture before analysis. Soil water
content was determined gravimetrically.
Following extraction, all samples were analyzed for inorganic N via
flow injection analysis. The respective isotopic ratios of NH4+ and
NO3- in the samples before and after incubation were quantified at the
U.C. Davis Isotope Lab from prepared diffused acidified disks (Stark
and Hart 1996). Blank solutions, as well as spiked solutions with
known enrichment and N mass, were diffused along with samples in order
to obtain an estimate of the quantity of N contamination, and
extraction efficiency, respectively, allowing a correction factor to
be applied to samples.
The equations of Kirkham and Bartholomew (1954) as presented in Hart
and others (1994), which use data on 14+15N and 15N in the initial and
incubated soil samples, and pool sizes of labeled pools, were used to
calculate gross rates of N mineralization (here ammonification),
nitrification, NH4+ immobilization, and NO3- immobilization. Gross N
immobilization was calculated as total inorganic N immobilization
(NH4+ + NO3-) minus gross nitrification.
All results are expressed on a per gram of dry soil basis. Values can
be converted to a “per area” basis using data on the mass of different
soil horizons found elsewhere on the data page of this website.